Abstract:
:The present study was designed to identify a possible new class of pathogen-recognition proteins that bind single-base oligodeoxynucleotide (ODN) ligands. Binding by the teleost natural killer cell equivalent [referred to as nonspecific cytotoxic cells (NCC)] was compared with mammalian cells (mouse RAW264.7 cells and human THP-1 cells). The ODN analysed were composed of 20-mers of guanosine (dG20), adenosine (dA20), thymidine (dT20) or cytosine (dC20). Binding studies first determined the 50% saturation levels for NCC (1.25 microg/ml), RAW264.7 (0.2 microg/ml) and THP-1 (0.8 microg/ml). Binding by dG20 to all the three cell types was saturable. Ligand blots of NCC membrane lysates with biotinylated dG20 revealed two different major molecular weight species (16-18 and 29 kDa) of binding proteins. The 29-kDa protein was identified with the help of Western blot analysis using a polyclonal antibody specific to an NCC antimicrobial protein (ncamp-1). The membrane expression of the 29-kDa ncamp-1 was determined by the binding of surface-biotinylated NCC membrane proteins with digoxigenin dG20 followed by immunoprecipitation using anti-digoxigenin agarose beads. The 29 and 14-18 kDa NCC membrane proteins were cross-reactive using Western blot examination with a polyclonal anti-histone 1 antibody. Function studies revealed that dG20 activated a twofold upregulation of membrane binding by homologous dG20-biotin. dG20 also stimulated NCC-increased membrane expression of NCC receptor protein 1. Additional experiments were performed to determine the DNase sensitivity of the different ODN. dG20 appeared to be more resistant to DNase treatment, compared to dC20, dA20 and dT20. The single-base ODN-binding proteins may represent a new class of pattern-recognition receptors that are involved in innate anti-bacterial resistance mediated by NCC.
journal_name
Scand J Immunoljournal_title
Scandinavian journal of immunologyauthors
Kaur H,Jaso-Friedmann L,Leary JH 3rd,Evans DLdoi
10.1111/j.0300-9475.2004.01455.xkeywords:
subject
Has Abstractpub_date
2004-09-01 00:00:00pages
238-48issue
3eissn
0300-9475issn
1365-3083pii
SJI1455journal_volume
60pub_type
杂志文章abstract::The immune system of a healthy individual responds vigorously to foreign microbial antigens. However, all potentially immunogenic regions (determinants) within an antigen are not functionally of equal relevance in mediating host immunity against the pathogen. Moreover, some of these antigenic determinants are well pro...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.2006.01891.x
更新日期:2007-02-01 00:00:00
abstract::Induced expression of the intercellular adhesion molecule 1 (ICAM-1) and of the major histocompatibility complex (MHC) class II antigens has been simultaneously observed on keratinocytes and epithelial cells in the thyroid and kidney, suggesting that ICAM-1 and HLA-DR expression might be under common regulation. We ha...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1991.tb03755.x
更新日期:1991-02-01 00:00:00
abstract::G-protein-coupled receptors play a major role in the activation of the innate immune system, such as polymorphonuclear neutrophils. Members of the formyl peptide receptor family recognize chemotactic peptides as well the amyloïd-beta peptide and fragments of the human immunodeficiency virus envelope and may thus be im...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.2005.01651.x
更新日期:2005-08-01 00:00:00
abstract::B-Lymphocyte-activating factor (BAFF/BLyS) is a survival factor for B cells, belonging to the tumor necrosis ligand super family. Serum BAFF levels have been found to be elevated in patients with systemic lupus erythematosus (SLE). Neutralization of BAFF activity was suggested as an additional therapeutic approach in ...
journal_title:Scandinavian journal of immunology
pub_type: 临床试验,杂志文章
doi:10.1111/j.1365-3083.2006.01737.x
更新日期:2006-04-01 00:00:00
abstract::Saliva was collected from 57 infants aged 6 weeks to 2.5 years and the molecular form of IgA was studied by centrifugation on sucrose density gradients. Two distinct populations were identified. Seventy-two per cent of the children had secretory IgA in their salivary secretions, while 28% had a molecular form correspo...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1989.tb01130.x
更新日期:1989-03-01 00:00:00
abstract::Peripheral blood lymphoid subsets expressing either CD45RA or CD29 antigens, were quantified in 30 children and 59 adults with Down Syndrome and appropriate age-matched controls, by dual immunofluorescence and flow cytometry. Down's patients, both adults and children, displayed a significant decrease of CD4+CD45RA+ ce...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1993.tb01733.x
更新日期:1993-10-01 00:00:00
abstract::BALB/c mice were injected with an affinity-chromatography-purified placental transferrin receptor preparation. Spleen cells were fused with NS-1 myeloma cells. Sixteen hybrids producing monoclonal antibodies specific for the transferrin receptor and two hybrids specific for transferrin were identified by radioimmunoas...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1984.tb01023.x
更新日期:1984-11-01 00:00:00
abstract::Mouse macrophages were cultured on chemically modified plastic dishes. On dishes covered with immobilized glycans, the macrophages were stimulated as judged by increased 14C-glucosamine incorporation, increased cytostatic and cytolytic capacities and by morphology as seen by scanning electron microscopy. The correspon...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1984.tb01013.x
更新日期:1984-10-01 00:00:00
abstract::Central events in the host defence system and immune-mediated damage are tightly regulated by cell adhesion molecules. Sera from 28 human immunodeficiency virus (HIV)-1 infected children divided into groups according to disease severity, six seroreverting (SR) children and 25 healthy controls were studied to detect th...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3083.2000.00820.x
更新日期:2000-12-01 00:00:00
abstract::The in vitro effect of superoxide anion and lysosomal enzyme activity on the killing of Listeria monocytogenes EGD (listeria) by peritoneal macrophages (PM) was investigated. Generation of superoxide anion by PM stimulated with phorbol myristate acetate (PMA) was significantly increased by intraperitoneal injection of...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1986.tb02131.x
更新日期:1986-10-01 00:00:00
abstract::Denaturing high-performance liquid chromatography (DHPLC) was evaluated as a tool for diagnostic screening of polymorphisms in the tumour necrosis factor receptor superfamily member 6 (TNFRSF6) also known as CD95, Apo-1 or Fas gene. Exons 1-9 of the TNFRSF6 gene were amplified from genomic DNA of 38 individuals, of wh...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.0300-9475.2004.01422.x
更新日期:2004-05-01 00:00:00
abstract::Cutaneous leishmaniasis (CL) is a self-healing skin disease which rarely for unknown reason(s) the lesion develops to a non-healing form. It seems that the initial contact of Leishmania parasites with the host innate immune system is an important step in the outcome of the disease. Recent studies suggested that toll-l...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/sji.12105
更新日期:2013-11-01 00:00:00
abstract::The effects of cytoplasmic anti-neutrophil cytoplasm autoantibodies (C-ANCA) and perinuclear ANCA (P-ANCA) immunoglobulin G (IgG) on tissue factor (TF) activity using HL-60 cells in vitro were compared with those of medium, lipopolysaccharide (LPS) and control IgG. Cells were also incubated with both ANCA IgG and cont...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3083.2003.01184.x
更新日期:2003-01-01 00:00:00
abstract::World Health Organisation recommends the practice of BCG vaccination at birth in countries which have a high incidence of tuberculosis and/or high leprosy burden. The BCG vaccination is considered safe for a competent immune system. However, in children with weakened immune systems cause of which can be primary or sec...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/sji.13010
更新日期:2020-12-16 00:00:00
abstract::The major rheumatoid factor cross-reactive idiotype, which was defined by prototypic monoclonal IgM rheumatoid factors from Waldenstrom's macroglobulinaemia patient Wa, is present on 60% of all monoclonal IgM RF paraproteins. One-third of patients with juvenile rheumatoid arthritis (JRA), who are seronegative for clas...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1991.tb01568.x
更新日期:1991-10-01 00:00:00
abstract::We have characterized a monoclonal antibody (AT389), produced by immunization of mice with 4-day-old avian Concanavalin A blasts, which recognizes a 38-40 kDa homodimer present on the surface of all chicken T cells. AT389 increased the spontaneous and IL-2-driven proliferation of 3-day-old T-cell blasts. This antibody...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1993.tb01707.x
更新日期:1993-08-01 00:00:00
abstract::The effect of low-dose methotrexate (MTX) treatment on the CD26 density on circulating monocytes and CD4(+) T lymphocytes or levels of soluble CD26 (sCD26) has not yet been described in rheumatoid arthritis (RA). While CD26 in T lymphocytes is involved in the activation and proliferation of T lymphocytes, little is kn...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.2007.01966.x
更新日期:2007-10-01 00:00:00
abstract::The initial stage of Wegener's Granulomatosis (WG) is often marked by symptoms of infection and it has been postulated that a bacterial infection could be the aetiologic factor of this disease. The objective of our work was to investigate T-cell-mediated immunity in WG by testing proliferative responses on bacterial a...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3083.1999.00544.x
更新日期:1999-06-01 00:00:00
abstract::Immature monocytes residing in the spleens of newborn mice less than 6-7 days of age are known to suppress T-dependent and T-independent immune responses in vitro and in vivo. Suppression is mediated in part through products secreted by the newborn monocytes. In the present study, we have identified 3 monokines secret...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1986.tb01940.x
更新日期:1986-01-01 00:00:00
abstract::Osteoclasts are responsible for bone destruction in rheumatoid arthritis (RA), and adipose-derived mesenchymal stromal cells (ADSCs) can inhibit experimental collagen-induced arthritis model. This study aims to determine whether ADSCs also suppresses osteoclastogenesis and bone erosion in collagen-induced arthritis (C...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/sji.12877
更新日期:2020-08-01 00:00:00
abstract::The in vitro effects of pokeweed mitogen (PWM) on axial organ (AO) cells of the echinoderm starfish Asteria rubens have been studied. PWM stimulates 3H-thymidine incorporation by the whole population of AO cells (index of stimulation 4-5). On the basis of surface adherence to nylon wool the AO cell population can be f...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1981.tb00565.x
更新日期:1981-09-01 00:00:00
abstract::The interferon-alpha and -beta (IFN-alpha/beta) producing ability of the two murine dendritic cell (DC) lines D2SC/1 and FSDC was studied. The D2SC/1 cells produced IFN-alpha and -beta when stimulated by herpes simplex virus (HSV), Sendai virus (SV) or by the bacteria Escherichia coli or Staphylococcus aureus Cowan I....
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3083.1997.d01-120.x
更新日期:1997-09-01 00:00:00
abstract::Adaptive immunity is a complex system that appeared twice in vertebrates (in gnathostomes and in jawless fish) although it is not required for invertebrate defence. The adaptive immune system is tightly associated with self-non-self discrimination, and it is now clear that this interplay is not limited to the preventi...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章,评审
doi:10.1111/sji.12348
更新日期:2015-11-01 00:00:00
abstract::Exogenously added beta 2-microglobulin (beta 2m) binds to a variety of murine cell types. The 'receptor' for beta 2m has been isolated. The purified 'receptor' comprised a 48,000-dalton chain and occasionally a 25,000-dalton component. Direct crosslinking of beta 2m to the receptor on intact cells gave rise to a singl...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1980.tb00014.x
更新日期:1980-01-01 00:00:00
abstract::The present investigation was designed to determine whether the heavy or the light immunoglobulin chain is involved in the non-immune binding of IgG F(ab')2 fragments to specific surface receptors on human group C and G streptococci. Purified human polyclonal IgG was mildly reduced with dithiothreitol and alkylated wi...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1985.tb01414.x
更新日期:1985-02-01 00:00:00
abstract::Galectin-3 has been suggested as a pro-inflammatory mediator in animal arthritis and rheumatoid arthritis (RA). We aimed to study the serum level of galectin-3 in patients with newly diagnosed RA and associations with disease profile, Magnetic resonance imaging (MRI) findings and seromarkers of synovial matrix inflamm...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/sji.12619
更新日期:2017-12-01 00:00:00
abstract::There are genetically determined differences in susceptibility to arthritis among inbred rat strains. The aim of the present study was to elucidate phenotypical differences, by determining expression of TNF and IL-1beta, two pivotal mediators of arthritis, in the highly arthritis-prone Dark Agouti (DA) rat compared to...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.2008.02210.x
更新日期:2009-03-01 00:00:00
abstract::Human endothelial cells (EC), cultured serum-free, synthesize de novo protein which increasingly bind to agarose beads (an alternative pathway activator), until a plateau phase is reached after 24-48 h. EC synthesize functional C3, C5, C6, C8 and C9, which were detected on co-cultured agarose beads, using relevant pol...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1991.tb02539.x
更新日期:1991-06-01 00:00:00
abstract::Human immunodeficiency virus (HIV) infection is a risk factor for thrombotic microangiopathy (TMA). We sought whether a severe deficiency in ADAMTS13, the enzyme specifically involved in the cleavage of von Willebrand factor, was associated with specific presenting features and outcome in HIV-associated TMA. In this p...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章,多中心研究
doi:10.1111/j.1365-3083.2008.02143.x
更新日期:2008-09-01 00:00:00
abstract::Propagermanium is an organic germanium compound with immunopotentiating activity. We examined the hepatoprotective effect of propagermanium and its mechanism in an experimental animal model of acute liver injury induced with Corynebacterium parvum (C. parvum) and lipopolysaccharide (LPS) injection. Oral pretreatment w...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3083.1998.00356.x
更新日期:1998-08-01 00:00:00