当前位置： SCI文献检索 > SCANDINAVIAN JOURNAL OF IMMUNOLOGY期刊下所有文献 > Human umbilical vein endothelial cells synthesize functional C3, C5, C6, C8 and C9 in vitro.

Human umbilical vein endothelial cells synthesize functional C3, C5, C6, C8 and C9 in vitro.

Abstract：

:Human endothelial cells (EC), cultured serum-free, synthesize de novo protein which increasingly bind to agarose beads (an alternative pathway activator), until a plateau phase is reached after 24-48 h. EC synthesize functional C3, C5, C6, C8 and C9, which were detected on co-cultured agarose beads, using relevant polyclonal anti-complement antibodies. Two monoclonal anti-C9 neoepitope antibodies (aE11, poly C9-MA) bound to the co-cultured beads, showing that the terminal complement complex (TCC) (C5b-9) was assembled on the beads. This also suggests that C7 is synthesized. There seems to be a positive correlation between the amount of agarose-bound labelled protein and agarose-bound complement. The results indicate that EC produce and secrete the components for the functional alternative and terminal pathways of complement.

journal_name

Scand J Immunol

journal_title

Scandinavian journal of immunology

authors

Johnson E,Hetland G

doi

10.1111/j.1365-3083.1991.tb02539.x

subject

Has Abstract

pub_date

1991-06-01 00:00:00

pages

667-71

issue

6

eissn

0300-9475

issn

1365-3083

journal_volume

33

pub_type

杂志文章

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