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The use of fluorometric assays to assess the immune response to DNA in murine systemic lupus erythematosus.

Abstract：

:Antibodies to DNA (anti-DNA) play an important role in the pathogenesis of systemic lupus erythematosus (SLE). In blood, these antibodies may exist in a free, unbound state or as part of complexes with DNA. Furthermore, circulating DNA may be either complexed or free. Because of the central role of these immunoreactants (anti-DNA and DNA) in the disease, monitoring of their levels could provide valuable information for both clinical and investigative purposes. In these studies, we have explored the use of a DNA-binding dye, PicoGreen, for the detection of circulating DNA, either total or immune complex bound. In addition, we have used this dye for Farr-type antibody assays. Using autoimmune MRL/lpr mice as a model, we have shown that, while the levels of free DNA in the plasma of these mice were comparable with those of normal BALB/c mice, the amounts in complexes precipitable by ammonium sulfate were significantly greater. Furthermore, we showed that Farr assays using PicoGreen reliably detect levels of free anti-DNA, with values correlated with anti-DNA levels by an enzyme-linked immunosorbent assay. Together, our results suggest that a fluorometric dye can accurately monitor DNA and anti-DNA antibody levels in SLE and may provide important information on immunopathogenesis.

journal_name

Scand J Immunol

journal_title

Scandinavian journal of immunology

authors

Björkman L,Reich CF,Pisetsky DS

doi

10.1046/j.1365-3083.2003.01261.x

keywords:

subject

Has Abstract

pub_date

2003-06-01 00:00:00

pages

525-33

issue

6

eissn

0300-9475

issn

1365-3083

pii

1261

journal_volume

57

pub_type

杂志文章

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