Abstract：
:In the present study we describe the production and characterization of a panel of monoclonal antibodies (MoAbs) directed against cruzipain (Crz), the major cysteine proteinase from Trypanosoma cruzi. The five MoAbs, BD6, BF2, CG2, CH8, and DC10 were analysed with respect to affinity and specificity. None of the MoAbs cross-reacted with papain, which has regions of high homology with Crz. Treatment of the antigen with periodate did not affect the binding of the MoAbs, suggesting that they bind to the polypeptide moiety of Crz. CH8 recognized a continuous epitope located at the C-terminal extension of the proteinase that appeared to be highly immunogenic. Although the rest of the MoAbs recognized epitopes located in the catalytic domain, the enzymatic activity of Crz was not impaired by the binding of the MoAbs. Characterization of the antibody-binding sites revealed the presence of at least four separate epitopes.
journal_name
Scand J Immunoljournal_title
Scandinavian journal of immunologyauthors
Gonzalez G,Orn A,Cazzulo JJ,Grönvik KOdoi
10.1111/j.1365-3083.1994.tb03479.xsubject
Has Abstractpub_date
1994-10-01 00:00:00pages
389-94issue
4eissn
0300-9475issn
1365-3083journal_volume
40pub_type
杂志文章abstract：:T cell-mediated autoimmune skin diseases develop as a result of the aberrant immune response to the skin cells with T cells playing a central role. These chronic inflammatory skin diseases encompass various types including psoriasis, lichen planus and vitiligo. These diseases show similarities in their immune-pathophy...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章,评审
doi：10.1111/sji.13000
更新日期：2020-11-15 00:00:00
abstract：:We have sequenced the variable heavy chain regions of a number of VH4-21 encoded monoclonal IgM anti-Rh(D) antibodies produced in response to deliberate immunization. These were compared with the sequences of similarly encoded IgM anti-I cold agglutinins (CA) derived from patients with lympho-proliferative diseases. T...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1995.tb03630.x
更新日期：1995-07-01 00:00:00
abstract：:The immunoglobulin (Ig) plasma levels are known to be, at least partially, genetically regulated, but all the genes involved are not known. Interleukin-1 (IL-1) is a potent proinflammatory cytokine able to serve as an adjuvant for immune responses. IL-1alpha gene is polymorphic, and at least one of the polymorphisms h...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.2003.01231.x
更新日期：2003-03-01 00:00:00
abstract：:Several enveloped viruses, particularly some RNA viruses, have high rates of mutation or replication, which can make them virulent pathogens in humans and other mammals. A proposed treatment could use synthesized proteins to mask pathogenic viral surface proteins to quickly induce an immune attack on specific envelope...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/sji.12928
更新日期：2020-09-01 00:00:00
abstract：:Coexistence of amyloid fibril protein AA and homogeneous immunoglobulin light-chain fragments was found in the isolated amyloid fibrils of two patients with amyloidosis secondary to rheumatoid arthritis. The light-chain amyloid fibril protein showed antigenic identity with a light-chain amyloid from a patient with pri...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1976.tb02989.x
更新日期：1976-01-01 00:00:00
abstract：:Human blood lymphocytes were tested for their proliferative response to purified protein derivative (PPD) in vitro. Purified T cells showed a lower response than the orginial purified lymphocyte suspension. Purified B cells were unresponsive to PPD. Mixtures of preparations of T and B cells were stimulated to a greate...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1975.tb02655.x
更新日期：1975-09-01 00:00:00
abstract：:In this review, we summarize and discuss some key findings from the study of naturally occurring autoantibodies. The B-cell compartment of the immune system appears to recognize almost all endogenous and environmental antigens. This ability is accomplished principally through autopolyreactive humoral and cellular immu...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章,评审
doi：10.1111/sji.12414
更新日期：2016-04-01 00:00:00
abstract：:The build-up of long-term immunological memory in the gut mucosal immune system may explain long-lasting protection against new attacks of cholera in convalescents from natural disease. We have looked for gut mucosal antitoxin immunological memory and memory cells in mice after oral immunization with cholera toxin. Ou...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1986.tb01995.x
更新日期：1986-05-01 00:00:00
abstract：:The objective of this study was to evaluate whether major abdominal surgery leads to complement activation and interleukin response and whether the kind of anaesthesia influence complement activation and the release of inflammatory interleukins. The study design was prospective and randomised. Fifty patients undergoin...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章,随机对照试验
doi：10.1111/j.1365-3083.2012.02672.x
更新日期：2012-05-01 00:00:00
abstract：:The T-cell regulatory function has been evaluated in lines of mice genetically selected according to the High (H) and Low (L) antibody responsiveness to heterologous erythrocytes. The inhibition of antibody production following CD4+ subset depletion was stronger in L than in H mice. The dose of CD4-specific monoclonal...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1995.tb03567.x
更新日期：1995-03-01 00:00:00
abstract：:Spleen cells from mice exposed to 500 rad sublethal whole-body irradiation before immunization with 100 x 10(6) allogeneic spleen cells exhibited an enhanced cytolytic T-lymphocyte (CTL) activity. In contrast, this treatment completely abrogated the humoral alloantibody response, as measured by complement-mediated lys...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1979.tb01384.x
更新日期：1979-01-01 00:00:00
abstract：:In this article we present methods for the purification and fractionation of human blood lymphocytes, which have been used in our laboratory to characterize antibody-dependent cytotoxic effector cells (K cells). The assay system consists of highly purified lymphocytes, 51Cr-labelled chicken erythrocytes (Ec) and IgG r...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1976.tb03856.x
更新日期：1976-06-01 00:00:00
abstract：:Human T lymphocytes, upon phytohaemagglutinin stimulation, are able to form colonies in semisolid media. Peripheral T cell bearing Fc receptors (TG) were studied for their possible regulatory activity on lymphoid colony development. Although no substantial differences were observed between the cloning efficiency of un...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1980.tb00099.x
更新日期：1980-01-01 00:00:00
abstract：:Flow cytometric analysis of splenocytes from mice infected with lymphocytic choriomeningitis virus revealed marked and long-standing up-regulation of LFA-1 expression on CD8+, but not on CD4+ T cells. Appearance of CD8+ T cells with a changed expression of adhesion molecules reflected polyclonal activation and expansi...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1995.tb03633.x
更新日期：1995-07-01 00:00:00
abstract：:TNF-alpha production has a central role in the development and progression of Pseudomonas aeruginosa septic shock. We have previously shown that P. aeruginosa slime-glycolipoprotein (slime-GLP) is the most potent stimulant compared to P. aeruginosa lipopolysaccharide (LPS), for TNF-alpha production and NF-kB activatio...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2007.02053.x
更新日期：2008-02-01 00:00:00
abstract：:Systemic lupus erythematosus is a systemic autoimmune disorder of unknown aetiology but is most likely caused by an interaction between several genetic factors and the environment. In a previously published genome scan we presented linkage to a marker on chromosome 4p13 in Icelandic families. Fine mapping of the regio...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2006.01810.x
更新日期：2006-09-01 00:00:00
abstract：:Antibodies against IL-1 alpha were detected in sera of apparently healthy individuals. The immunoglobulins belonged to the IgG class, particularly IgG1, IgG2, and IgG4. [125I]rIL-1 alpha bound to Fab fragments of IgG, and IgG immune complexes of molecular weights from 160 to 700 kDa were formed in the sera by [125I]rI...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1990.tb03212.x
更新日期：1990-12-01 00:00:00
abstract：:The activation of complement and the release of TNF-alpha, IL-6 and IL-8 are important pathogenic factors behind organ dysfunction in sepsis. The aim of this study was to determine whether infusion of anti-TNF antibodies alters complement activation and plasma concentrations of pro-inflammatory cytokines at high doses...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.1998.00433.x
更新日期：1998-11-01 00:00:00
abstract：:To delineate accurately the IgG subclass distribution of thyroid auto-antibodies, sera from nine patients with Hashimoto's thyroiditis were fractionated into IgG subclasses by complete depletion of the other IgG subclasses on affinity columns. All IgG subclass fractions contained thyroglobulin and microsomal (or thyro...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1989.tb01190.x
更新日期：1989-07-01 00:00:00
abstract：:Conditions for the cloning of alloactivated human lymphocytes by limiting dilution in the presence of interleukin 2 (IL 2) and filler cells have been investigated. Cloning efficiencies were extremely high (at least 40%) when IL 2 derived from pooled peripheral blood mononuclear cells (PBM), stimulated with phytohaemag...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：
更新日期：1981-11-01 00:00:00
abstract：:alpha 2-macroglobulin (alpha 2M) preparations purified from the plasma of healthy persons and gastric carcinoma patients were further analysed by immunochemical techniques. It was found that only alpha 2M from the plasma of gastric carcinoma patients gave a positive reaction with E-receptor sheep antiserum in rocket i...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1991.tb02543.x
更新日期：1991-06-01 00:00:00
abstract：:Peripheral blood mononuclear cells (PBM) from four patients with IgG myeloma and four patients with benign monoclonal gammopathy (BMG) were stimulated with pokeweed mitogen (PWM), and the in vitro immunoglobulin production over 7 days was measured with an enzyme-linked immunosorbent assay. All myeloma patients were su...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1984.tb00911.x
更新日期：1984-02-01 00:00:00
abstract：:Acanthamoeba polyphaga (AP) is ubiquitous in nature and frequently infects humans. AP has some features, such as persistence, which makes it an attractive candidate in studies of a possible infectious aetiology in rheumatoid arthritis (RA). In this study the occurrence of AP-specific antibodies was compared between RA...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.2001.00926.x
更新日期：2001-06-01 00:00:00
abstract：:Six synthetic peptides of the MHC class I molecule corresponding to individual H-2Kb participants in amino acid sequences of domains alpha 1 (peptide 1 and 2) and alpha 2 (peptides 3, 4, 5, 6) were selected. Kb-specific suppressor T cells (Ts) were induced in vivo in mice, then pretreated with a set of peptides and as...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1993.tb01674.x
更新日期：1993-06-01 00:00:00
abstract：:B-cell-dependent autoantibody production is a hallmark of systemic lupus erythematosus (SLE) which requires costimulatory molecules. The aim of the study was to analyse the expression of costimulatory molecules on B cells in patients with SLE. Twenty-six patients with SLE (four male, 22 female, mean age 46 +/- 15 year...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2007.02008.x
更新日期：2007-11-01 00:00:00
abstract：:Phosphorothioate oligodeoxynucleotides (sODNs) can induce T-cell-independent polyclonal activation of human B cells by a mechanism that depends on both sequence and back-bone structure. Because matrix-bound as well as soluble sODNs are mitogenic, this stimulation may result from the engagement of surface receptor(s). ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.2001.01004.x
更新日期：2001-12-01 00:00:00
abstract：:The monoclonal antibodies A and B specific for the HN molecule of Sendai virus were used to induce polyclonal and monoclonal anti-idiotypic antibodies. No response was observed in allogeneic Lewis rats, low responses in syngeneic LOU rats, and high responses in allogeneic BN rats and xenogeneic Balb/c mice. The monocl...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1986.tb02103.x
更新日期：1986-09-01 00:00:00
abstract：:Mononuclear cells (MNC) eluted from rheumatoid synovial tissue of 16 patients with rheumatoid arthritis were examined for immunoglobulin-secreting cells (ISC). Immediately after elution and separation synovial tissue MNC contained considerable numbers of ISC. IgG and IgA ISC were more abundant than IgM ISC. At the sam...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1982.tb00741.x
更新日期：1982-11-01 00:00:00
abstract：:(C3H/TifxDBA/2)F1 mice, immunized with viable BALB/c plasmacytoma MOPC315 cells, produce antibodies directed against a cell-surface antigen. The strain and tissue distribution of this antigen was identical to that of the plasma cell differentiation alloantigen PC.1. The antigen is absent in the mouse strain C3H/Tif bu...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1979.tb03176.x
更新日期：1979-01-01 00:00:00
abstract：:The T-cell repertoire is shaped by the positive and negative selection of immature CD4(+) CD8(+) double positive (DP) thymocytes. Positive selection of DP T cells to the CD4(+) CD8(-) and CD4(-) CD8(+) simple positive (SP) lineages is a multistep process which involves cellular interactions between thymocytes and stro...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.2000.00818.x
更新日期：2000-12-01 00:00:00
