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A solid-phase radioimmunoassay for C1q-binding immune complexes. I. Delta IgG as indicator molecule.

Abstract：

:A solid-phase radioimmunoassay for detection of circulating C1q-binding immune complexes (IC) or heat-aggregated IgG (delta IgG) is described. Purified human C1q protein was adsorbed to a fixed area of polystyrene tube surface for 1 hr at 22 degrees C, pH 7.3, mu 0.15. Binding of delta IgG or IC to solid-phase C1q at 22 degrees C progressed over several hours and was enhanced at low mu (0.05). Heating of C1q (56 degrees C for 30 min) reduced the binding by 85%-90%. Binding of IC or delta IgG was retained after several weeks' storage of solid-phase C1q at 4 degrees C. Detection of 2-5 ng delta IgG and less than 50 ng IC (in antibody excess) was achieved in competitive binding or inhibition tests with [125I]delta IgG. Preliminary testing of 48 human sera indicated the usefulness of the assay for detection of IC in patient sera.

journal_name

Scand J Immunol

journal_title

Scandinavian journal of immunology

authors

Svehag SE

doi

10.1111/j.1365-3083.1975.tb02677.x

keywords:

subject

Has Abstract

pub_date

1975-01-01 00:00:00

pages

687-97

issue

7

eissn

0300-9475

issn

1365-3083

journal_volume

4

pub_type

杂志文章

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