Abstract:
:The occurrence of D-D recombination during the embryonic differentiation of chicken B cells was studied. Ig heavy (H) chains were amplified by polymerase chain reaction from day-12 bursal cDNA, and 30 random V-D-J regions were analysed by DNA sequencing. No gene conversion events were observed in any of the V regions, indicating that diversification of the H chains by gene conversion is not yet activated at this stage of embryonic B-cell development. In contrast, the V-D-J joint regions were extremely heterogeneous. Most of the sequenced V-D-J joints were formed by direct joining of the single-germline V mu 1 gene, one of the multiple-germline D elements, and the single J gene. However, three V-D-J regions were clearly longer in size, and their D-region structure indicated recombination between two or three different germline D elements. Thus, the present data suggest that D-D recombination may have a role in diversification of the Ig H-chain repertoire.
journal_name
Scand J Immunoljournal_title
Scandinavian journal of immunologyauthors
Mansikka A,Toivanen Pdoi
10.1111/j.1365-3083.1991.tb02524.xsubject
Has Abstractpub_date
1991-05-01 00:00:00pages
543-8issue
5eissn
0300-9475issn
1365-3083journal_volume
33pub_type
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