Abstract:
:Interleukin-1 (IL-1) production by the human monocyte-like cloned cell line CM-SM has been investigated as a function of the state of cell differentiation. CM-SM cells were induced to differentiate along the monocyte-macrophage lineage by bacterial lipopolysaccharides (LPS) or by 12-O-tetradecanoyl-phorbol-13-acetate (TPA). Cell differentiation was studied by various morphological, functional, cytochemical, and immunological variables, whereas IL-1 activity in the supernatants was measured by the lectin-primed thymocyte proliferation assay. Unstimulated CM-SM cells constitutively produced small amounts of IL-1, and most of the cells appeared relatively undifferentiated. LPS induced cell differentiation, but the effect was reversible, and the cells, in general, did not acquire a capacity for phagocytosis. IL-1 levels were increased about 10-fold over the controls. TPA induced further cell differentiation to macrophage-like cells capable of phagocytosis. IL-1 activity could not be measured directly in the supernatants owing to the synergistic effect of TPA in the assay system. Unequivocal removal of the phorbol was not achieved, but the data indicated that the 'real' levels of IL-1 in the TPA-induced cultures were not significantly higher than those from LPS-induced cultures.
journal_name
Scand J Immunoljournal_title
Scandinavian journal of immunologyauthors
Revoltella RP,Butler RH,Musiani P,Piantelli M,Ranelletti FOdoi
10.1111/j.1365-3083.1985.tb01397.xsubject
Has Abstractpub_date
1985-01-01 00:00:00pages
11-20issue
1eissn
0300-9475issn
1365-3083journal_volume
21pub_type
杂志文章abstract::Multifactorial involvement in the pathogenesis of autoimmune NZB/W F1 mice has been well documented. To further elucidate the role of cytokines in the disease development of murine lupus, single spleen cells isolated from NZB/W F1 and non-autoimmune C57BL/6 mice were stimulated with T cell mitogens or anti-CD3 antibod...
journal_title:Scandinavian journal of immunology
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journal_title:Scandinavian journal of immunology
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journal_title:Scandinavian journal of immunology
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journal_title:Scandinavian journal of immunology
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1983.tb00778.x
更新日期:1983-02-01 00:00:00
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journal_title:Scandinavian journal of immunology
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journal_title:Scandinavian journal of immunology
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journal_title:Scandinavian journal of immunology
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
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更新日期:1987-10-01 00:00:00
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journal_title:Scandinavian journal of immunology
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更新日期:1987-06-01 00:00:00
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:
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journal_title:Scandinavian journal of immunology
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更新日期:2020-12-15 00:00:00
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journal_title:Scandinavian journal of immunology
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journal_title:Scandinavian journal of immunology
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journal_title:Scandinavian journal of immunology
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更新日期:1980-01-01 00:00:00
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
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更新日期:1997-01-01 00:00:00
abstract::Allergic airway disorders such as asthma and allergic rhinitis are mainly caused by inhaled allergen-induced improper activation and responses of immune and non-immune cells. One important response is the production of IL-27 by macrophages and dendritic cells (DCs) during the early stage of airway allergies. IL-27 exe...
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journal_title:Scandinavian journal of immunology
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
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更新日期:2011-12-01 00:00:00
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章,评审
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更新日期:2000-01-01 00:00:00
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1978.tb00514.x
更新日期:1978-01-01 00:00:00
abstract::The possible immunological relationship between the pattern of Th1/Th2 cytokine production and tuberculin reactivity was assessed in patients with active Mycobacterium tuberculosis infection. The production of the intracellular cytokines interferon (IFN)-gamma and interleukin-4 (IL-4) was measured in CD4(+) and CD8(+)...
journal_title:Scandinavian journal of immunology
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更新日期:2007-03-01 00:00:00
abstract::Our data on peripheral blood T cells from Motor neuron disease (MND) patients indicate major immunological disturbances linked to this disease. Both CD4+ and CD8+ T-cell subsets display an increased fraction of cells showing activation markers compared to controls, indicating an unusually high level of activity in bot...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3083.2001.00923.x
更新日期:2001-07-01 00:00:00