Abstract:
:Cells produced in murine T-cell colonies grown in methylcellulose have been studied to evaluate their proliferation, phenotypic differentiation, and ability to give rise to cytotoxic cells. Colony-forming cells from presumably cortical or medullary thymocytes, separated by peanut agglutination, or from lymph nodes, showed rapid proliferation regardless of their origin, although cortical thymic colony cells showed significantly slower proliferation from day 10 to day 12 of culture. Essentially all the colony cells maintained the Lyt-1+2+ phenotype of the colony-forming cells, and no L3T4+ cells were found. The colony cells were characterized as rather mature cells by being strongly H-2-positive, and, except for a few cells, by lacking the TL antigen. Colony cells maintained the Lyt-1+2+ phenotype after expansion in liquid culture, and both cortically and medullary derived colony cells showed lectin-dependent cytotoxic activity against P-815 tumour cells. We conclude that our assay for growing thymus-derived T-cell colonies results in the proliferation of a phenotypically and functionally relatively mature cytotoxic T-lymphocyte progeny.
journal_name
Scand J Immunoljournal_title
Scandinavian journal of immunologyauthors
Röpke C,Claësson MHdoi
10.1111/j.1365-3083.1986.tb01963.xsubject
Has Abstractpub_date
1986-02-01 00:00:00pages
243-50issue
2eissn
0300-9475issn
1365-3083journal_volume
23pub_type
杂志文章abstract::Guinea pig alpha 1-microglobulin (alpha 1-m) was found to exert mitogenic effects on guinea pig lymphocytes, i.e. peritoneal exudate lymphocytes (PEL) or T lymphocytes from regional or mesenteric lymph nodes (LNL). On the other hand, spleen T lymphocytes did not respond to alpha 1-m. Stimulation of LNL required the pr...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1986.tb02173.x
更新日期:1986-11-01 00:00:00
abstract::Sea star factor (SSF) harvested from the echinoderm Asterias forbesi celomocytes is a potent inhibitor of the primary immune response to T-dependent antigens and also suppresses concanavalin-A-induced mitogenesis. We have isolated and purified a basic protein from this source which has a molecular weight of approximat...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1976.tb03037.x
更新日期:1976-01-01 00:00:00
abstract::We have recently shown that a heat treatment of a murine target cell line, WEHI 164, induces resistance to lysis mediated by tumour necrosis factors alpha (TNF-alpha) and beta (TNF-beta). In the present study the effect of the heat shock of target cells on cytotoxicity mediated by natural killer cells (NK cells), lymp...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1990.tb02757.x
更新日期:1990-02-01 00:00:00
abstract::Here we analyse the ability of soluble immune complexes (IC) prepared with cationized antibodies to induce cytotoxic responses mediated by neutrophils. While cationized IC induced high levels of cytotoxicity, control IC induced very low levels of response. Inhibition of cytotoxicity by catalase but not by three haemen...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1993.tb01755.x
更新日期:1993-02-01 00:00:00
abstract::T cell-mediated autoimmune skin diseases develop as a result of the aberrant immune response to the skin cells with T cells playing a central role. These chronic inflammatory skin diseases encompass various types including psoriasis, lichen planus and vitiligo. These diseases show similarities in their immune-pathophy...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章,评审
doi:10.1111/sji.13000
更新日期:2020-11-15 00:00:00
abstract::Interactions between homing receptors on circulating leucocytes and endothelial addressins regulate tissue-specific cellular extravasation. Although integrin alpha 4 beta 7 appears to be the main receptor for gut-homing T lymphocytes, less is known about molecules mediating mucosal B cell homing. Expression of integri...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1995.tb03709.x
更新日期:1995-12-01 00:00:00
abstract::TNF-alpha production has a central role in the development and progression of Pseudomonas aeruginosa septic shock. We have previously shown that P. aeruginosa slime-glycolipoprotein (slime-GLP) is the most potent stimulant compared to P. aeruginosa lipopolysaccharide (LPS), for TNF-alpha production and NF-kB activatio...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.2007.02053.x
更新日期:2008-02-01 00:00:00
abstract::Susceptibility to autologous and heterologous neutralization of primary human immunodeficiency virus (HIV)-1 isolates belonging to subtype B, to the B"-variant of subtype B or to subtype F from infected individuals residing in Rio de Janeiro was assayed. A lower infectivity of the B"- and F isolates when compared to t...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3083.1998.00345.x
更新日期:1998-06-01 00:00:00
abstract::With the use of the fluorescent Ca++ indicator Quin-2, we have measured changes in intracellular calcium levels in human B cells in response to anti-Ig antibodies, to Staphylococcus aureus (Staph) or to protein A. Cells of an Epstein-Barr virus-transformed mu lambda-carrying B-cell line, AZU-1, increased free cytosoli...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1985.tb01915.x
更新日期:1985-11-01 00:00:00
abstract::Heterogeneity in antigen recognition of an alloreactive helper T-cell line was studied by repeated limiting dilution cloning. Analysis of the fine specificities of these clones showed that the gain of reactivity to new antigens by the T-cell line included the generation of non-cross-reactive T cells specific to new ma...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1988.tb02398.x
更新日期:1988-06-01 00:00:00
abstract::The aim of this study was to evaluate possible changes in the circulating levels of interferon (IFN)-gamma, interleukin (IL)-4 and transforming growth factor (TGF)-beta in association with the autoimmune process leading to type 1 diabetes. Expression levels of mRNAs specific for each cytokine were determined in periph...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3083.2001.00904.x
更新日期:2001-05-01 00:00:00
abstract::Deficiency of complement components within the classical pathway is associated with increased risk for immune complex disease. However, C2-deficient individuals often have a mild disease and about 50% are healthy. To study the importance of the different components for immune complex clearance, bovine serum albumin (B...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3083.2000.00752.x
更新日期:2000-07-01 00:00:00
abstract::The effects of cytoplasmic anti-neutrophil cytoplasm autoantibodies (C-ANCA) and perinuclear ANCA (P-ANCA) immunoglobulin G (IgG) on tissue factor (TF) activity using HL-60 cells in vitro were compared with those of medium, lipopolysaccharide (LPS) and control IgG. Cells were also incubated with both ANCA IgG and cont...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3083.2003.01184.x
更新日期:2003-01-01 00:00:00
abstract::Around 20 susceptibility loci for type 1 diabetes mellitus (T1DM) have been mapped. One of these loci, IDDM10, was found on chromosome 10p11-q11. Here, we investigated whether the IDDM10 locus contributes in the susceptibility to T1DM in a Russian family dataset. One hundred and fourteen simplex Russian families, each...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.0300-9475.2004.01480.x
更新日期:2004-09-01 00:00:00
abstract::The reactivity of rabbit anti-HLA-DR antigen antibodies with cells in normal and rheumatoid synovial tissue was investigated by indirect immunofluorescence on frozen sections of tissue. The antibodies reacted with a significant proportion of the synovial lining cells of both normal and rheumatoid synovial tissue, with...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1981.tb00198.x
更新日期:1981-08-01 00:00:00
abstract::The effect of trypsin on mouse spleen cells and enriched B cells, added alone or together with lipopolysaccharide (LPS), was investigated. With trypsin, proliferation in serum free spleen cell cultures was 2-6 times greater than the background using cells from LPS responder strains, and 2-4 times the background with c...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1989.tb01110.x
更新日期:1989-02-01 00:00:00
abstract::The thymus is the major primary immune tissue for the production of functional T lymphocytes in vertebrates. However, its evolutionary origin is unknown. It has recently been shown that the generation of local T cells also occurs in gut-associated lymphoid tissues (GALT). This suggests that the thymus and GALT have si...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章,评审
doi:10.1046/j.1365-3083.2001.00854.x
更新日期:2001-01-01 00:00:00
abstract::As part of an ongoing project aimed at the characterization of the MHC system in bony fishes, we have attempted to identify the class III region in the zebrafish (a teleost), a region that in higher vertebrates contains genes coding for complement proteins C2, Bf and C4. We obtained several genomic PAC clones by hybri...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3083.1998.00457.x
更新日期:1998-12-01 00:00:00
abstract::Mast-cell degranulation is triggered by the bridging of Fc receptor-bound antigen-specific immunoglobulin IgE on the cell surface. In vitro experiments suggest that antibody affinity and nonspecific IgE may affect the mast-cell function, however, their importance in vivo is unclear. Investigations of the effects of th...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3083.2002.01012.x
更新日期:2002-01-01 00:00:00
abstract::Phagocytosis of Staphylococcus aureus by human polymorphonuclear leucocytes (PMN) on the surface of endothelial cells is accompanied by adherence of the PMN to the endothelial surface and detachment of the endothelial cells from the culture monolayer. We studied the role of the leucocyte adherence-related glycoprotein...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1989.tb01192.x
更新日期:1989-07-01 00:00:00
abstract::We have studied the effect of interferon in vivo (induced by polyinosinic-polycytidylic acid (pIC] on the natural killer (NK) cell lytic activity and on the numbers of large granular lymphocytes (LGL) and target-binding cells (TBC) in different lymphoid compartments. One day after the pIC induction the spleen containe...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1984.tb01016.x
更新日期:1984-10-01 00:00:00
abstract::KIR2DL2 and KIR2DL3 are important inhibitory receptors that recognize a subset of HLA-C allelic products carrying Ser77 and Asn80. In this study, we have determined KIR2DL2 and KIR2DL3 diversity in the Chinese Han population by a PCR sequence-based typing. Based on sequencing, the coding regions of 166 Chinese Han ind...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/sji.12277
更新日期:2015-04-01 00:00:00
abstract::To study the role of Natural Killer (NK) cells in Leishmania infection, peritoneal macrophages from BALB/c mice were infected with Leishmania (Leishmania) amazonensis promastigotes and incubated with interleukin-2 (IL-2)-activated NK (A-NK) cells at different ratios of A-NK cells to infected macrophages (5:1, 1:1, 0.2...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.2005.01681.x
更新日期:2005-10-01 00:00:00
abstract::This paper describes the generation, detection and characterization of cytotoxic t lymphocyte activity in vitro and the application of this assay system to various immunological problems. ...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1976.tb03864.x
更新日期:1976-06-01 00:00:00
abstract::After intravenous injection of 10(5) purified, lymph node (LN)-derived dm2 (H-2d/Ld-) CD4+ T cells into young C.B-17 scid/scid (severe combined immunodeficiency, SCID) mice (H-2d/Ld+), the transplanted Ld-T cells show a selective pattern of engraftment: they repopulate the spleen, the lamina propria of the small intes...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1993.tb01694.x
更新日期:1993-07-01 00:00:00
abstract::The mixed lymphocyte culture (MLC) response of lymphocytes from CBA mice against C3H cells was studied after injection of spleen cells from C3H mice or C3H hybrids. Intravenous infusion of C3H cells resulted in a strongly suppressed specific MLC response, but this was not the case when cells from H-2-incompatible hybr...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1975.tb02619.x
更新日期:1975-01-01 00:00:00
abstract::Cutaneous lupus erythematosus (CLE) is an autoimmune disease with a broad range of cutaneous manifestations. In skin lesions of CLE, keratinocytes primarily undergo apoptosis. Interferon-κ(IFN-κ) is belonged to type I interferons (type I IFNs) and is selectively produced by keratinocytes. Recently, keratinocytes selec...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章,评审
doi:10.1111/sji.12933
更新日期:2021-01-01 00:00:00
abstract::Direct contact between lymphokine-activated killer (LAK) cells and tumour cells is believed to be imperative for initiating tumour cell lysis in vitro as well as in vivo. In order to optimize adoptive immunotherapy (AIT) it is therefore desirable to identify the LAK cell subtype, which ensures maximal infiltration of ...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3083.1998.00339.x
更新日期:1998-06-01 00:00:00
abstract::Prior studies of classical 24 h responses in TNP-Cl (picryl chloride) allergic contact sensitivity (CS), showed mediation by Th1 cells in CBA mice, and established that 24 h elicitation of responses requires an early 2 h CS-initiating component dependent on iNKT cells, IL-4 and B-1 B cells. Here, we studied the other ...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.2011.02522.x
更新日期:2011-05-01 00:00:00
abstract::The relationship between the so-called natural interferon-alpha (IFN-alpha) producing cell (IPC), stimulated to produce IFN-alpha by herpes simplex virus type 1 (HSV), and of dendritic cells (DC) in peripheral blood leucocytes was investigated. The simultaneous expression of cell surface antigens and intracellular IFN...
journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3083.1996.d01-289.x
更新日期:1996-08-01 00:00:00