当前位置： SCI文献检索 > SCANDINAVIAN JOURNAL OF IMMUNOLOGY期刊下所有文献 > C-reactive protein triggers calcium signalling in human neutrophilic granulocytes via FcγRIIa in an allele-specific way.

C-reactive protein triggers calcium signalling in human neutrophilic granulocytes via FcγRIIa in an allele-specific way.

Abstract：

:C-reactive protein (CRP) binds to Fcγ-receptors, FcγRIIa (CD32) with high affinity and to FcγRIa (CD64) with low affinity. The binding to CD32 has been shown to be allele specific, that is, it binds to R/R131 but not to H/H131. Little is known about the cooperation of CRP and neutrophilic granulocytes (PMNs) in inflammatory reactions. The purpose of the present study was to examine CRP signalling in human PMNs, and whether this signalling is also allele specific. Cytosolic calcium of PMN was measured in a single-cell digital imaging system. Receptor expression and polymorphism were studied by real-time RT-PCR, flow cytometry and standard PCR. C-reactive protein induced cytosolic calcium signals in PMNs from homozygote R/R131 donors, but not in PMNs from heterozygote R/H131 donors. However, after the heterozygote PMNs had been incubated with IFN-γ (100 U/ml) for 2 h, both the proportion of cells responding and the size of the CRP-induced calcium signals increased. IFN-γ increased mRNA expression of CD64 about fivefold and surface protein expression of CD64 about fourfold. The calcium signal elicited by CRP was augmented by PMN adhesion to fibronectin, but almost totally abrogated by sphingosine kinase inhibitors. The signals were partly dependent on calcium influx. In conclusion, calcium signalling instigated by CRP in human PMN is FcγRIIa allele specific, as R/R131 responded to CRP, whereas R/H131 did not. However, increased expression of FcγRIa (CD64), stimulated by IFN-γ, can augment calcium signalling by CRP in low-responders. This suggests that the state of the PMNs, as well as the genetic origin, affect sensitivity for CRP.

journal_name

Scand J Immunol

journal_title

Scandinavian journal of immunology

authors

Aas V,Sand KL,Asheim HC,Benestad HB,Iversen JG

doi

10.1111/sji.12049

subject

Has Abstract

pub_date

2013-06-01 00:00:00

pages

442-51

issue

6

eissn

0300-9475

issn

1365-3083

journal_volume

77

pub_type

杂志文章

相关文献

SCANDINAVIAN JOURNAL OF IMMUNOLOGY文献大全
  • Acute phase serum amyloid A protein increases high density lipoprotein binding to human peripheral blood mononuclear cells and an endothelial cell line.

    abstract：:Serum Amyloid A (SAA) is an acute-phase protein secreted mainly by hepatocytes and is largely associated with high-density lipoprotein (HDL) in plasma. It has been suggested that SAA alters HDL binding to the cell surface and that this in turn changes HDL-mediated cholesterol delivery to cells. Incorporation of SAA in...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-3083.2000.00661.x

    authors： Hayat S,Raynes JG

    更新日期：2000-02-01 00:00:00

  • Mononuclear cell factors that inhibit fibroblast collagen synthesis. II. Properties of the factors.

    abstract：:Supernatants harvested from peripheral blood mononuclear cells (PBMC) incubated either with the non-specific mitogen phytohaemagglutinin-P (PHA) or with the specific antigen tuberculin purified protein derivative for 72 h decreased collagen synthesis by dermal fibroblasts. PHA-induced mononuclear cell factors (PHA-MCF...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1983.tb00835.x

    authors： Hart PH,Powell LW,Cooksley WG,Halliday JW

    更新日期：1983-07-01 00:00:00

  • Defective Candida opsonin and procidin function in patients with recurrent pyogenic infection: a common immunodeficiency.

    abstract：:Polymorphonuclear cell function tests were performed in 340 patients with recurrent unexplained infections. Serum-dependent defects in phagocytosis and intracellular killing of Candida albicans (Candida opsonin and procidin defects) were specifically associated with susceptibility to recurrent pyogenic infections, Can...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1991.tb02495.x

    authors： Parkin JM,Bridges J,Sloper KS,Warner JO,Pinching AJ

    更新日期：1991-01-01 00:00:00

  • Over-estimation of the number of complement receptor type 1 (CR1) on erythrocytes.

    abstract：:The number of CR1 on erythrocytes, as measured by monoclonal antibodies, remains undefined because of the repetitive structure of CR1 and the presence of different types of structural CR1 alleles. We studied the number of CR1 per erythrocyte using two monoclonal antibodies, E11 and 3D9, which recognize different sites...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1992.tb02948.x

    authors： Quadri RA,Schifferli JA

    更新日期：1992-07-01 00:00:00

  • Peritoneal B cells differentiate without proliferation into autoantibody secretors under the influence of factors released by other cells.

    abstract：:After 3-5 days of in vitro culture, peritoneal cells from untreated C3H mice produce autoantibodies specific for autoantigens in the membranes of mouse erythrocytes. The autoantigens are exposed in vitro by treating mouse erythrocytes with the proteolytic enzyme bromelain. Limiting-dilution cell culture techniques wer...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1984.tb01035.x

    authors： Cox KO,Daenke S,Samcewicz B

    更新日期：1984-12-01 00:00:00

  • Immunological effects of D-penicillamine during experimental induced inflammation in rats.

    abstract：:Administration of D-penicillamine (50 mg/kg/day orally) to rats with adjuvant arthritis for up to 42 days significantly modified the incorporation of 3H-thymidine (3H-TdR) in concanavalin A (Con A)-stimulated lymph node cells. Treatment with D-penicillamine abolished the ability of macrophages from arthritic rats to i...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1980.tb00064.x

    authors： Binderup L,Bramm E,Arrigoni-Martelli E

    更新日期：1980-01-01 00:00:00

  • Ex vivo responses for interferon-gamma and proinflammatory cytokine secretion to low-molecular-weight antigen MTB12 of Mycobacterium tuberculosis during human tuberculosis.

    abstract：:MTB12 protein, also called CFP-2, is a major and early secreted component of Mycobacterium tuberculosis. However, its role during mycobacterial infection has been poorly characterized. In this study, we purified the native MTB12 protein and investigated the profile of MTB12-induced cytokines [interferon (IFN)-gamma, t...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.2006.01784.x

    authors： Lee JS,Son JW,Jung SB,Kwon YM,Yang CS,Oh JH,Song CH,Kim HJ,Park JK,Paik TH,Jo EK

    更新日期：2006-08-01 00:00:00

  • D-D recombination diversifies the CDR 3 region of chicken immunoglobulin heavy chains.

    abstract：:The occurrence of D-D recombination during the embryonic differentiation of chicken B cells was studied. Ig heavy (H) chains were amplified by polymerase chain reaction from day-12 bursal cDNA, and 30 random V-D-J regions were analysed by DNA sequencing. No gene conversion events were observed in any of the V regions,...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1991.tb02524.x

    authors： Mansikka A,Toivanen P

    更新日期：1991-05-01 00:00:00

  • Generation of anti-tumour effector T cells from naïve T cells by stimulation with dendritic/tumour fusion cells.

    abstract：:Tumour-draining lymph node T cells are an excellent source of effector T cells that can be used in adoptive tumour immunotherapy because they have already been sensitized to tumour-associated antigens in vivo. However, such tumour-specific immune cells are not readily obtained from the host due to poor immunogenicity ...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.2007.02012.x

    authors： Ishida A,Tanaka H,Hiura T,Miura S,Watanabe S,Matsuyama K,Kuriyama H,Tanaka J,Kagamu H,Gejyo F,Yoshizawa H

    更新日期：2007-11-01 00:00:00

  • Long-lasting immune response induced by recombinant bacillus Calmette-Guérin (BCG) secretion system.

    abstract：:The recombinant bacillus Calmette-Guérin (rBCG) secretion system utilizing an extracellular alpha antigen of Mycobacterium kansasii (alpha-K) was characterized biochemically and immunologically. The human immunodeficiency virus type 1 (HIV-1) p17gag B cell epitope fused to alpha-K was secreted in extremely large amoun...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-3083.1996.d01-28.x

    authors： Wada N,Ohara N,Kameoka M,Nishino Y,Matsumoto S,Nishiyama T,Naito M,Yukitake H,Okada Y,Ikuta K,Yamada T

    更新日期：1996-02-01 00:00:00

  • Medial histocompatibility antigens.

    abstract：:Medial histocompatibility (H) antigens are weak H antigens, recognized by unrestricted T cells; they differ thus from both major and minor H antigens. An example, Qed-1, is described in detail, and other known medial H antigens of the mouse are reviewed. The structural and genetic relationships of major and medial H a...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章,评审

    doi：10.1111/j.1365-3083.1981.tb00607.x

    authors： Lindahl KF,Langhorne J

    更新日期：1981-12-01 00:00:00

  • The complex role of vitamin D in autoimmune diseases.

    abstract：:Vitamin D, besides having well-known control functions of calcium and phosphorus metabolism, bone formation and mineralization, also has a role in the maintenance of immune-homeostasis. The immune-regulatory role of vitamin D affects both the innate and adaptive immune system contributing to the immune-tolerance of se...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章,评审

    doi：10.1111/j.1365-3083.2008.02127.x

    authors： Szodoray P,Nakken B,Gaal J,Jonsson R,Szegedi A,Zold E,Szegedi G,Brun JG,Gesztelyi R,Zeher M,Bodolay E

    更新日期：2008-09-01 00:00:00

  • Ligands of surface Ig raise cytoplasmic free Ca++ in human B cells.

    abstract：:With the use of the fluorescent Ca++ indicator Quin-2, we have measured changes in intracellular calcium levels in human B cells in response to anti-Ig antibodies, to Staphylococcus aureus (Staph) or to protein A. Cells of an Epstein-Barr virus-transformed mu lambda-carrying B-cell line, AZU-1, increased free cytosoli...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1985.tb01915.x

    authors： Clevers HC,Bloem AC,Gmelig-Meyling F,Ballieux RE

    更新日期：1985-11-01 00:00:00

  • Enhancement of cytotoxic activity of natural killer cells by interleukin 2, and antagonism between interleukin 2 and adenosine cyclic monophosphate.

    abstract：:Two cytokines, interferon (IFN) and interleukin 2 (IL-2), activate murine natural killer (NK) cells in vitro. Together both factors synergize considerably. Antibody against IFN eliminates the response of NK cells to IFN as well as to IL-2, whereas antibody against IL-2 blocks the effect of IL-2 but not of IFN. These f...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1985.tb01895.x

    authors： Chun M,Krim M,Granelli-Piperno A,Hirst JA,Hoffmann MK

    更新日期：1985-10-01 00:00:00

  • The relation between cutaneous blood flow and cell content in the tuberculin reaction.

    abstract：:The relationship of velocity of blood flow to density and microanatomical distribution of inflammatory cells in the dermis was studied in 20 human tuberculin tests. Most positive reactions showed maximal blood flow velocities (measured as red blood cell (RBC) flux) at the centre of the reaction, but the two most inten...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1989.tb01096.x

    authors： Swanson Beck J,Gibbs JH,Potts RC,Jawad ES,Kardjito T,Spence VA,Grange JM

    更新日期：1989-01-01 00:00:00

  • Prethymic progenitors from the avian para-aortic mesoderm express GATA-3 and distinct chTcf isoforms but still lack T-cell receptor-gamma rearrangements.

    abstract：:Haematopoietic precursors first colonizing the avian embryonic thymus are derived from the intraembryonic sites located around the dorsal aortae. These intraembryonic precursors have previously been demonstrated to include cells that harbour T-cell progenitor capacity and express the Ikaros transcription factor, known...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-3083.2000.00807.x

    authors： Liippo J,Koskela K,Lassila O

    更新日期：2000-11-01 00:00:00

  • CD45 engagement induces L-selectin down-regulation.

    abstract：:The CD45 glycoprotein isoforms exhibit a receptor-like composition and display intracellular protein tyrosine phosphatase (PTPase) activity. The present study links CD45 to the regulation of L-selectin (CD62L), a leucocyte glycoprotein important for extravasation and homotypic aggregation. Monoclonal antibodies (MoAbs...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-3083.1996.d01-282.x

    authors： Stibenz D,Bührer C,Laufer D,Obladen M

    更新日期：1996-07-01 00:00:00

  • Chronic granulamatous disease: Two decades of experience from a paediatric immunology unit in a country with high rate of consangineous marriages.

    abstract：:Chronic granulomatous disease (CGD) is a primary immunodeficiency characterized by susceptibility to bacterial and fungal infections resulting from the inadequacy of phagocytic leucocytes to produce reactive oxygen radicals. CGD is a genetically heterogeneous disease with an X-linked recessive (XR-CGD) form caused by ...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/sji.12737

    authors： Kutukculer N,Aykut A,Karaca NE,Durmaz A,Aksu G,Genel F,Pariltay E,Cogulu Ö,Azarsız E

    更新日期：2019-02-01 00:00:00

  • Acidic protein in macrophages.

    abstract：:An antiserum to unstimulated rat peritoneal macrophages was produced in rabbits. The antibodies were directed against an acidic protein with a molecular weight of 35,000 and with an isoelectric point at 4.6. The macrophage acidic protein (MAP) was purified by gel filtration of rat lung soluble proteins, followed by pr...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1981.tb00576.x

    authors： Persson LI,Hansson E,Rönnbäck L

    更新日期：1981-10-01 00:00:00

  • An alternative perspective to the immune response in autoimmune exocrinopathy: induction of functional quiescence rather than destructive autoaggression.

    abstract：:Sjögren's syndrome is characterized by dryness of the eyes and the mouth due to mononuclear cell infiltration of the lacrimal and salivary glands. The aetiology is unknown but autoimmunity is considered to play a significant role in the pathogenesis. Recent studies have focused on the fact that tear and salivary flow ...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章,评审

    doi：10.1046/j.1365-3083.1999.00490.x

    authors： Humphreys-Beher MG,Brayer J,Yamachika S,Peck AB,Jonsson R

    更新日期：1999-01-01 00:00:00

  • B- and T-cell-specific alloantigens in man.

    abstract：:Screening for B-cell-specific antibodies in unabsorbed pregnancy sera preselected for weak reactivity in regular HLA-A, -B, and -C screening yielded a relatively high proportion (35/81) of B-cell-specific antibodies. Most B-cell-specific antibodies react broadly, showing inclusion phenomena suggesting 'cross-reactivit...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1977.tb02099.x

    authors： Albert ED,Andreas A,McNicholas A,Scholtz S,Kuntz B

    更新日期：1977-01-01 00:00:00

  • C1-inhibitor reduces the ischaemia-reperfusion injury of skeletal muscles in mice after aortic cross-clamping.

    abstract：BACKGROUND:Both C1-inhibitor (C1-INH) and antibodies against the CD18 adhesion molecule have been shown to reduce ischaemia-reperfusion injuries. The objective of this study was to investigate the effect of increased ischaemia times and to determine whether inhibiting C1 or blocking the CD18 function was protective in ...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-3083.2002.01173.x

    authors： Nielsen EW,Mollnes TE,Harlan JM,Winn RK

    更新日期：2002-12-01 00:00:00

  • Comparison between the specificity of primary and secondary killer cells against alloantigens and hapten-modified syngeneic lymphoid cells.

    abstract：:Cytotoxic responses of lymphoid cells from different mouse strains against syngeneic cells modified with the haptens fluorescein isothiocyanate and trinitrophenyl were investigated. Mic of the H-2k strain demonstrated strong primary in vitro hapten-specific cytotoxicity reactions, which were H-2 restricted and involve...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1981.tb00119.x

    authors： Möller E,Ramos T

    更新日期：1981-01-01 00:00:00

  • Idiotype-specific, major histocompatibility complex restricted T cells are of both Th1 and Th2 type.

    abstract：:The lymphokine secretion patterns of seven independent 91-101.lambda 2(315)/I-Ed specific, CD4+ T-cell clones have been investigated. Six of the clones are of the Th1 type as they secrete IL2 and IFN gamma, but not IL4. Some of these six Th1 clones produce TNF alpha/beta, and some produce minor amounts of IL5 and IL6....

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1991.tb02537.x

    authors： Lauritzsen GF,Bogen B

    更新日期：1991-06-01 00:00:00

  • IL-23 Activated γδ T Cells Affect Th17 Cells and Regulatory T Cells by Secreting IL-21 in Children with Primary Nephrotic Syndrome.

    abstract：:This study (1) analysed the percentage of γδ T cells, γδ T cell subsets, Th17 cells and regulatory T cells (Treg cells) and (2) determined the role of IL-23 in primary nephrotic syndrome (PNS) patients with active disease and in remission. Eighty-four patients with PNS and 51 healthy age-matched controls were included...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/sji.12629

    authors： Zhang L,Yan J,Yang B,Zhang G,Wang M,Dong S,Liu W,Yang H,Li Q

    更新日期：2018-01-01 00:00:00

  • Immune balance in Hepatitis B Infection: Present and Future Therapies.

    abstract：:Chronic hepatitis B virus (HBV) infection affects millions of people worldwide and about half a million people die every year. India represents the second largest pool of chronic HBV infections with an estimated 40 million chronically infected patients. Persistence or clearance of HBV infection mainly depends upon hos...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章,评审

    doi：10.1111/sji.12553

    authors： Vyas AK,Jindal A,Hissar S,Ramakrishna G,Trehanpati N

    更新日期：2017-07-01 00:00:00

  • Extensive CDR3H length heterogeneity exists in bovine foetal VDJ rearrangements.

    abstract：:Analysis of seven variable-diversity-joining (VDJ) gene rearrangements in B splenocytes from a 125-day-old bovine foetus revealed an extensive heavy-chain complementarity-determining region 3 (CDR3H) length variation (9-56 codons). Indeed, the global CDR3H size spectratyping of foetal VDJ rearrangements substantiated ...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-3083.2002.01028.x

    authors： Saini SS,Kaushik A

    更新日期：2002-02-01 00:00:00

  • Contribution of stabilizing agents present in intravenous immunoglobulin preparations to modulation of mononuclear cell proliferation in vitro.

    abstract：:Intravenous immunoglobulin (IVIG) preparations have been shown to suppress lymphocyte proliferation in vitro. This study aimed to investigate the effects of an IVIG produced by fractionation/DEAE-Sephadex adsorption on lymphocyte proliferation in vitro, with particular reference to contributions of the stabilizing age...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-3083.1996.d01-350.x

    authors： Alder LB,Morgan LA,Spickett GP

    更新日期：1996-12-01 00:00:00

  • Histamine secretion from human leucocytes stimulated by basophil-derived platelet-activating factor.

    abstract：:Crude preparations of platelet-activating factors (PAF) derived by methanolic extraction of supernatants from antigen-challenged basophils generally stimulated the secretion of histamine from human peripheral blood leucocytes when co-incubated with these cells in the absence of an additional stimulator. When these cru...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1981.tb00168.x

    authors： O'Donnell MC,Fiedel BA

    更新日期：1981-01-01 00:00:00

  • Upregulated microRNA-132 in T helper 17 cells activates hepatic stellate cells to promote hepatocellular carcinoma cell migration in vitro.

    abstract：:MicroRNAs play an important role in the modulation of the immune system. T helper 17 (Th17) cells are involved in the modulation of the tumour microenvironment. However, the function of miRNA in Th17 cells in the tumour microenvironment is unclear. In this study, we analysed miR-132 expression in Th17 cells and assess...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/sji.13007

    authors： Feng R,Cui Z,Liu Z,Zhang Y

    更新日期：2020-12-02 00:00:00