当前位置： SCI文献检索 > SCANDINAVIAN JOURNAL OF IMMUNOLOGY期刊下所有文献 > Interferon-gamma production in antigen specific T cell response: quantitation of specific mRNA and secreted protein.

Interferon-gamma production in antigen specific T cell response: quantitation of specific mRNA and secreted protein.

Abstract：

:Interferon gamma (IFN-gamma) production as a measure of cellular sensitization was studied by detection of the cytokine in culture supernatant by enzyme immunoassay (EIA) and by measuring cellular mRNA using the reverse transcriptase polymerase chain reaction (RT-PCR) method. These assays were compared to the standard lymphocyte proliferation assay as a marker of T cell responsiveness to foreign antigens. When blood donors seropositive for herpes simplex virus (HSV) were compared to seronegative donors, all measurements of cellular sensitization separated the groups without overlap. There were significant correlations between the IFN-gamma mRNA titre and the secreted IFN-gamma (r = 0.57, P = 0.03), and the proliferative response and the secreted IFN-gamma (r = 0.78, P = 0.001), as well as between the IFN-gamma mRNA titre and the proliferative response (r = 0.78, P < 0.001). When tetanus toxoid (TT) responses were studied in immunized subjects, a wide range of responsiveness could be seen and correlation between various measurements was poor. However, constant individual levels of the cytokine production were demonstrated. Six people who had received their last TT booster vaccination more than 5 years ago were revaccinated and repeatedly studied. An increase in the levels of produced IFN-gamma could be seen in all subjects and two who lacked a lymphocyte proliferation response developed it after revaccination.

journal_name

Scand J Immunol

journal_title

Scandinavian journal of immunology

authors

Halminen M,Klemetti P,Vaarala O,Hurme M,Ilonen J

doi

10.1046/j.1365-3083.1997.d01-144.x

subject

Has Abstract

pub_date

1997-10-01 00:00:00

pages

388-92

issue

4

eissn

0300-9475

issn

1365-3083

journal_volume

46

pub_type

杂志文章

相关文献

SCANDINAVIAN JOURNAL OF IMMUNOLOGY文献大全
  • Interferon-mediated enhancement of thyroid major histocompatibility complex antigen expression. A flow cytometric analysis.

    abstract：:Epithelial expression of class II antigens encoded by the major histocompatibility complex (MHC) has been proposed as a means by which autoimmune thyroid disease may be initiated and maintained. We studied a rat thyroid epithelial cell line (FRTL-5), which constitutively expresses class I (OX18) but not class II (OX6 ...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1987.tb01088.x

    authors： Rayner DC,Lydyard PM,de Assis-Paiva HJ,Bidey S,van der Meide P,Varey AM,Cooke A

    更新日期：1987-06-01 00:00:00

  • Isolation and partial characterization of a murine cell surface glycoprotein with affinity for exogenously added beta 2-microglubulin.

    abstract：:Exogenously added beta 2-microglobulin (beta 2m) binds to a variety of murine cell types. The 'receptor' for beta 2m has been isolated. The purified 'receptor' comprised a 48,000-dalton chain and occasionally a 25,000-dalton component. Direct crosslinking of beta 2m to the receptor on intact cells gave rise to a singl...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1980.tb00014.x

    authors： Sege K,Peterson PA

    更新日期：1980-01-01 00:00:00

  • Xylaria hypoxylon Lectin as Adjuvant Elicited Tfh Cell Responses.

    abstract：:Foot-and-mouth disease (FMD) caused by FMD virus (FMDV) is a major health and economic problem in the farming industry. Vaccination of livestock against this highly infectious viral disease is crucial, and inactivated FMD vaccine has been effective at controlling infection. However, accumulated data show that the inac...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/sji.12349

    authors： Kang J,Zuo Y,Guo Q,Wang H,Liu Q,Liu Q,Xia G,Kang Y

    更新日期：2015-11-01 00:00:00

  • Amplification of T-cell and antibody responses in DNA-based immunization with HIV-1 Nef by co-injection with a GM-CSF expression vector.

    abstract：:Intradermal inoculation of mice with naked plasmid DNA encoding the regulatory HIV-1 Nef protein was shown to induce Nef-specific T and B cell responses. Co-inoculation with an expression vector encoding murine granulocyte-macrophage colony-stimulating factor (GM-CSF), a cytokine known to facilitate the induction of p...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-3083.1997.d01-130.x

    authors： Svanholm C,Löwenadler B,Wigzell H

    更新日期：1997-09-01 00:00:00

  • Recombinant Mycobacterium smegmatis expressing an ESAT6-CFP10 fusion protein induces anti-mycobacterial immune responses and protects against Mycobacterium tuberculosis challenge in mice.

    abstract：:The currently used vaccine against tuberculosis, Bacille Calmette-Guérin (BCG), has variable efficacy, so new vaccine development is crucial. In this study, we evaluated a recombinant vaccine prepared from non-pathogenic Mycobacterium smegmatis (rMS) that expresses a fusion of early secreted antigenic target 6-kDa ant...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.2010.02448.x

    authors： Zhang H,Peng P,Miao S,Zhao Y,Mao F,Wang L,Bai Y,Xu Z,Wei S,Shi C

    更新日期：2010-10-01 00:00:00

  • Measurement of IgE on rat mast cells: relation to serum IgE and allergen-induced histamine release.

    abstract：:IgE antibodies against trinitrophenylated ovalbumin (TNP-OVA) were raised in Brown Norway rats by a highly reproducible immunization procedure. With these rats, the kinetics of the IgE response was studied, both for free circulating and cell-bound IgE. Cell-bound IgE was dissociated from peritoneal cell suspensions at...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1983.tb00760.x

    authors： van Toorenenbergen AW,van Swieten P,Aalberse RC

    更新日期：1983-01-01 00:00:00

  • Cellular interactions between synovial T-cell clones from a patient with rheumatoid arthritis.

    abstract：:The aim of this study was to investigate T-T cell interactions that may be involved in the pathogenesis of chronic synovitis. Two CD4+ T-cell clones, termed N16 and N18, sharing similar cytokine profiles were isolated from the synovial tissue of a patient with rheumatoid arthritis. Activated N16 cells induced prolifer...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1993.tb01678.x

    authors： Van Laar JM,Miltenburg AM,De Kuiper P,Daha MR,De Vries RR,Breedveld FC

    更新日期：1993-06-01 00:00:00

  • Detection by three cellular immunological techniques of the antigenic determinants of the Ly-Li system, expressed on human B lymphocytes.

    abstract：:A clear correlation was observed between the presence of an Ia-like antigenic B-cell system Ly-Li, detected serologically, and three cellular immunological techniques: [1] mixed lymphocyte reaction (MLR) inhibition by an anti-Li antiserum; [2] level of restimulation of anti-Ly-Li in-vitro-primed lymphocytes; and [3] d...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1977.tb02112.x

    authors： Sasportes M,Mawas C,Crosier P,Charmot D,Legrand L,Dausset J

    更新日期：1977-01-01 00:00:00

  • Induction of cellular immune reactions by A36, an antigen complex of Mycobacterium paratuberculosis: comparison of A36 and johnin components.

    abstract：:Paratuberculosis (Johne's disease) is a chronic enteritis syndrome of ruminants, which is due to infection by Mycobacterium paratuberculosis. Cutaneous testing with proteins extracted from a mycobacterial culture fluid (johnin-PPD) is currently used to evaluate the cellular immune status. We have compared the componen...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1992.tb03143.x

    authors： Gilot P,De Kesel M,Coene M,Cocito C

    更新日期：1992-12-01 00:00:00

  • Down-regulation of L-selectin surface expression by various leukocyte isolation procedures.

    abstract：:L-selectin, a cell surface glycoprotein expressed on lymphocytes, granulocytes, and monocytes, has been implicated in lymphocyte homing and extravasation of phagocytic leukocytes into areas of inflammation. Considerable differences of L-selectin expression among various individuals has been reported, with clinical cor...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：

    authors： Stibenz D,Bührer C

    更新日期：1994-01-01 00:00:00

  • Stress and substance P but not the substance P-metabolite SP5-11 trigger murine abortion by augmenting TNF-alpha levels at the feto-maternal interface.

    abstract：:In a well-established murine abortion model, stress is thought to trigger fetal rejection by inducing a proinflammatory immune response via substance P (SP), being tumour necrosis factor (TNF)-alpha-producing CD8+ T cells involved. Interestingly, the SP metabolite SP5-11 also binds to SP receptors and mediates SP-like...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.2005.001711.x

    authors： Fest S,Zenclussen AC,Joachim R,Hagen E,Demuth HU,Hoffmann T

    更新日期：2006-01-01 00:00:00

  • Effect of pentoxifylline on levels of pro-inflammatory cytokines during chronic hepatitis C.

    abstract：:The cellular and humoral natural immune response induced by hepatitis C virus (HCV) is commonly unable to eradicate the virus. HCV is a highly mutable, hepatotropic RNA virus that causes acute and chronic hepatitis, an infection that involves the production of various cytokines. The aim of the study is to analyse the ...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.2006.001761.x

    authors： Gutierrez-Reyes G,Lopez-Ortal P,Sixtos S,Cruz S,Ramirez-Iglesias MT,Gutierrez-Ruiz MC,Sanchez-Avila F,Roldan E,Vargas-Vorackova F,Kershenobich D

    更新日期：2006-06-01 00:00:00

  • Transcriptional regulation of serum amyloid A1 gene expression in human aortic smooth muscle cells involves CCAAT/enhancer binding proteins (C/EBP) and is distinct from HepG2 cells.

    abstract：:Regulation of acute-phase serum amyloid A (A-SAA) synthesis by proinflammatory cytokines and steroid hormones in human aortic smooth muscle cells (HASMCs) is distinct from that in HepG2 cells. To study the cis- and trans-activating promoter element involved in the SAA1 gene expression by HASMCs and HepG2 cells, we con...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-3083.2002.01169.x

    authors： Kumon Y,Suehiro T,Faulkes DJ,Hosakawa T,Ikeda Y,Woo P,Sipe JD,Hashimoto K

    更新日期：2002-11-01 00:00:00

  • Role of Eosinophil Granulocytes in Allergic Airway Inflammation Endotypes.

    abstract：:Eosinophil granulocytes are intriguing members of the innate immunity system that have been considered important defenders during parasitic diseases as well as culprits during allergy-associated inflammatory diseases. Novel studies have, however, found new homoeostasis-maintaining roles for the cell. Recent clinical t...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章,评审

    doi：10.1111/sji.12448

    authors： Amin K,Janson C,Bystrom J

    更新日期：2016-08-01 00:00:00

  • Adhesive interactions between human NK cells and porcine endothelial cells.

    abstract：:Human natural killer (NK) cells are able to adhere to xenogeneic porcine endothelial cells (EC) and evidence from in vitro studies as well as animal models suggests a potential role for NK cells in the cellular recognition and damage of porcine xenogeneic tissues. One possible explanation for the observed NK cell-medi...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章,评审

    doi：10.1046/j.1365-3083.2001.00966.x

    authors： Schneider MK,Forte P,Seebach JD

    更新日期：2001-07-01 00:00:00

  • Peripheral circulating activated b-cell populations are associated with nephritis and disease activity in patients with systemic lupus erythematosus.

    abstract：:B-cell-dependent autoantibody production is a hallmark of systemic lupus erythematosus (SLE) which requires costimulatory molecules. The aim of the study was to analyse the expression of costimulatory molecules on B cells in patients with SLE. Twenty-six patients with SLE (four male, 22 female, mean age 46 +/- 15 year...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.2007.02008.x

    authors： Dolff S,Wilde B,Patschan S,Dürig J,Specker C,Philipp T,Kribben A,Witzke O

    更新日期：2007-11-01 00:00:00

  • Different glycosylation pattern of human IgG1 and IgG3 antibodies isolated from transiently as well as permanently transfected cell lines.

    abstract：:The effector functions of IgG depend on the presence of carbohydrates attached to asparagine 297 in the Fc-portion. In this report, glycosylation profiles of recombinant wild-type and mutant IgG1 and IgG3 antibodies produced from three cell lines were analysed using LC-ESI-Orbitrap. Clear differences were detected bet...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/sji.12046

    authors： Vestrheim AC,Moen A,Egge-Jacobsen W,Bratlie DB,Michaelsen TE

    更新日期：2013-05-01 00:00:00

  • Human schistosomiasis: deficiency of large granular lymphocytes and indomethacin-sensitive suppression of natural killing.

    abstract：:Twenty-eight children infected with Schistosoma haematobium and S. mansoni were tested for natural killer (NK) cell activity in vitro using the myeloid/erythroid cell line K562 as target. In addition, the frequency of large granular lymphocytes (LGL) and the number of HNK-1+ lymphocytes were examined in peripheral blo...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1986.tb01973.x

    authors： Gastl GA,Feldmeier H,Kortmann C,Daffalla AA,Peter HH

    更新日期：1986-03-01 00:00:00

  • Substrate specificity of the human lymphokine leucocyte migration-inhibitory factor (LIF): radioenzymic assay and inhibition by cGMP.

    abstract：:The human lymphokine, leucocyte migration-inhibitory factor (LIF), appears to be a serine esterase and protease by virtue of its susceptibility to the irreversible enzyme inhibitor, phenylmethylsulfonyl fluoride (PMSF), and by the ability of arginine esters and amides to protect LIF against PMSF-induced inactivation. ...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1979.tb01335.x

    authors： Bendtzen K

    更新日期：1979-01-01 00:00:00

  • Ligands of surface Ig raise cytoplasmic free Ca++ in human B cells.

    abstract：:With the use of the fluorescent Ca++ indicator Quin-2, we have measured changes in intracellular calcium levels in human B cells in response to anti-Ig antibodies, to Staphylococcus aureus (Staph) or to protein A. Cells of an Epstein-Barr virus-transformed mu lambda-carrying B-cell line, AZU-1, increased free cytosoli...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1985.tb01915.x

    authors： Clevers HC,Bloem AC,Gmelig-Meyling F,Ballieux RE

    更新日期：1985-11-01 00:00:00

  • Immunoglobulin G complexes without sialic acids enhance osteoclastogenesis but do not affect arthritis-mediated bone loss.

    abstract：:Immunoglobulin G (IgG) is important in clearance and recognition of previously presented antigens and after activation, IgGs can interact with the Fc gamma receptors (FcγRs) on haematopoietic cells, including bone-resorbing osteoclasts. The pathogenicity of IgG, that is the ability to elicit stimulatory effects via Fc...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/sji.13009

    authors： Sehic E,Westerlund A,Lagerquist MK,Lerner UH,Carlsten H,Henning P,Engdahl C

    更新日期：2020-12-15 00:00:00

  • CD25 blockade protects T cells from activation-induced cell death (AICD) via maintenance of TOSO expression.

    abstract：:CD25 monoclonal antibody binding to the alpha-chain of the Interleukin-2 (IL-2) receptor, blocks high-affinity IL-2 binding, thereby preventing complete T-cell activation and being of ample importance in transplantation medicine and potentially the treatment of autoimmune disease. However, CD25 antibodies do not only ...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.2009.02281.x

    authors： Richter GH,Mollweide A,Hanewinkel K,Zobywalski C,Burdach S

    更新日期：2009-09-01 00:00:00

  • Irregular cytokine pattern of CD4+ T lymphocytes in response to Staphylococcus aureus in patients with Wegener's granulomatosis.

    abstract：:The initial stage of Wegener's Granulomatosis (WG) is often marked by symptoms of infection and it has been postulated that a bacterial infection could be the aetiologic factor of this disease. The objective of our work was to investigate T-cell-mediated immunity in WG by testing proliferative responses on bacterial a...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-3083.1999.00544.x

    authors： Mayet WJ,Märker-Hermann E,Schlaak J,Meyer Zum Büschenfelde KH

    更新日期：1999-06-01 00:00:00

  • Identity of HML-1 antigen on intestinal intraepithelial T cells and of B-ly7 antigen on hairy cell leukaemia.

    abstract：:Immunoprecipitation of radioiodinated hairy cell leukaemia (HCL) cell lysates with monoclonal antibody (MoAb) HML-1, originally reported to recognize intraepithelial T cells, and with MoAb B-ly7, originally reported to react with HCL, led to identical biochemical characteristics. In SDS-PAGE under reducing conditions,...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1990.tb02896.x

    authors： Moldenhauer G,Mielke B,Dörken B,Schwartz-Albiez R,Möller P

    更新日期：1990-08-01 00:00:00

  • Immune response in asymptomatic paraproteinemia. I. Primary and secondary antibody response to Helix pomatia hemocyanin.

    abstract：:In a group of 20 patients with asymptomatic paraproteinemia, as judged after at least 3 years of follow-up, the primary and secondary antibody response to Helix pomatia hemocyanin (HPH) was defective as compared with the response in controls. The class of antibody was assessed by mercaptoethanol (ME) treatment of seru...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：

    authors： Weits J,de Gast GC,The TH,Marrink J,Mandema E

    更新日期：1976-01-01 00:00:00

  • Immunoglobulin A and CD8 T-cell mucosal immune defenses protect against intranasal infection with Chlamydia pneumoniae.

    abstract：:Chlamydia pneumoniae initiates infection in humans via the mucosal epithelia of the respiratory tract; therefore, immunity at this mucosal site is believed to be important to control infection with this pathogen. We compared the protective capacity of immunization in mice with two C. pneumoniae antigens, namely the ma...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.2006.01725.x

    authors： Rodríguez A,Rottenberg M,Tjärnlund A,Fernández C

    更新日期：2006-03-01 00:00:00

  • Development of tuberculosis vaccines in clinical trials: Current status.

    abstract：:Tuberculosis (TB) is an important infectious disease worldwide. Currently, Bacillus Calmette-Guérin (BCG) remains the only TB vaccine licensed for human use. This TB vaccine is effective in protecting children against severe military TB but offers variable protective efficacy in adults. Therefore, new vaccines against...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章,评审

    doi：10.1111/sji.12710

    authors： Méndez-Samperio P

    更新日期：2018-10-01 00:00:00

  • Increased N-linked glycosylation leading to oversialylation of monomeric immunoglobulin A1 from patients with Sjögren's syndrome.

    abstract：:Increased serum immunoglobulin A (IgA) level is a common finding in primary Sjögren's syndrome (pSS). IgA might not be properly eliminated because of an abnormal glycosylation. We reported previously that IgA1 from patients with pSS was oversialylated. We extend this finding by showing that monomeric IgA1 contains mor...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-3083.2000.00685.x

    authors： Basset C,Durand V,Jamin C,Clément J,Pennec Y,Youinou P,Dueymes M,Roitt IM

    更新日期：2000-03-01 00:00:00

  • Adhesion molecules involved in protein kinase A- and C-dependent lymphocyte adherence to microvascular endothelial cells.

    abstract：:A twofold increase in lymphocyte adherence to rat microvascular endothelial cells (EC) was achieved by incubating EC for 4 h with IL-1 alpha or dibutyryl-cAMP (stimulators of protein kinase A, PKA) and PMA (stimulator of protein kinase C, PKC). Monoclonal antibodies anti-CD11a, anti-CD18 (LFA-1) and anti-CD49d (VLA-4 ...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-3083.1993.tb02555.x

    authors： Turunen JP,Ustinov J,Renkonen R

    更新日期：1993-03-01 00:00:00

  • Peyer's patches in murine AIDS: dissociation between lymphoproliferation and anergy.

    abstract：:RadLV-Rs infection induces a murine immunodeficiency syndrome associated with a dramatic enlargement of spleen and lymph nodes. Surprisingly, the lymphoproliferation excludes thymus and Peyer's patches (PP). To understand the cellular interactions underlying lymphoproliferation further, the authors investigated the fa...

    journal_title：Scandinavian journal of immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-3083.1997.d01-390.x

    authors： Colombi S,Moutschen M,de Leval L,Demoitié MA,Trebak M,Martin MT,Boniver J

    更新日期：1997-02-01 00:00:00