Abstract:
:The human lymphokine, leucocyte migration-inhibitory factor (LIF), appears to be a serine esterase and protease by virtue of its susceptibility to the irreversible enzyme inhibitor, phenylmethylsulfonyl fluoride (PMSF), and by the ability of arginine esters and amides to protect LIF against PMSF-induced inactivation. In this paper, three methods are described by which putative substrates for LIF may be investigated. Thus, molecules satisfying the substrate specificities of this lymphokine should (1) protect LIF against inactivation by PMSF, (2) reduce LIF activity in vitro on polymorphonuclear leucocytes, and (3) reduce the esterolytic activity of purified LIF-rich supernatants. The first two reactions were tested by means of the leucocyte migration agarose technique; the third reaction was tested by a sensitive enzyme assay using tritiated tosyl arginine methyl ester as substrate. Guanosine 3',5'-cyclic monophosphoric acid, which is capable of protecting LIF against PMSF-induced inhibition, also inhibited the esterolytic activity of the purified LIF preparation. Four synthetic oligopeptide substrates for trypsin, thombin and plasmin were investigated. Only one, the thrombin- and trypsin-specific benzoyl-phenylalanyl-valyl-agarine-p-nitroanilide, possessed high affinity for the LIF molecule and may therefore prove to be a potent substrate for this lymphokine.
journal_name
Scand J Immunoljournal_title
Scandinavian journal of immunologyauthors
Bendtzen Kdoi
10.1111/j.1365-3083.1979.tb01335.xsubject
Has Abstractpub_date
1979-01-01 00:00:00pages
61-7issue
1eissn
0300-9475issn
1365-3083journal_volume
10pub_type
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journal_title:Scandinavian journal of immunology
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journal_title:Scandinavian journal of immunology
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journal_title:Scandinavian journal of immunology
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journal_title:Scandinavian journal of immunology
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
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更新日期:1990-12-01 00:00:00
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
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更新日期:2007-11-01 00:00:00
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
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更新日期:1978-01-01 00:00:00
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
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更新日期:2008-10-01 00:00:00
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章,评审
doi:10.1111/sji.12897
更新日期:2020-09-01 00:00:00
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1993.tb02571.x
更新日期:1993-05-01 00:00:00
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journal_title:Scandinavian journal of immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3083.1993.tb01708.x
更新日期:1993-08-01 00:00:00