Effect of interferon on cell proliferation and generation of cytotoxic potential in mixed autologous and allogeneic lymphocyte cultures.

Abstract:

:Autologous and allogeneic mixed lymphocyte cultures (AMC and MLC) were assayed for blastogenesis, generation of cytotoxic potential, and the effect of interferon (IFN-alpha) on these features. The cells of the mixed cultures lysed K562, Daudi, and autologous and allogeneic phytohaemagglutinin blasts. Stimulator-specific cytotoxicity was observed only in MLC. B blasts induced with Staphylococcus aureus were only affected in a stimulator-specific manner. Short-term IFN treatment of the MLC-derived effectors before the lytic assay enhanced the nonspecific component of cytotoxicity. Cell proliferation was considerably lower in AMC than in MLC. This was decreased when IFN-alpha was added at the initiation of the cultures. The presence of IFN influenced the generation of lytic potential. Comparison of the lysis of the different targets exerted by MLC-activated cells suggested that the specific component was more substantially elevated than the nonspecific one. It is likely that the IFN induced such modifications in the culture conditions that favoured the proliferation of the specific clone. Re-exposure of lymphocytes cultured in the presence of IFN to another dose of IFN before the assay had no influence on their lytic potential.

journal_name

Scand J Immunol

authors

Argov S,Klein E

doi

10.1111/j.1365-3083.1983.tb00784.x

subject

Has Abstract

pub_date

1983-03-01 00:00:00

pages

211-23

issue

3

eissn

0300-9475

issn

1365-3083

journal_volume

17

pub_type

杂志文章
  • Study of the mechanism for in vitro activation of mouse NK cells by interferon.

    abstract::The enhancement of the lytic capacity of mouse splenic 'natural killer' (NK) cells by interferon has been studied in vitro as a model for NK cell differentiation from inactive immediate precursors. We show that the increased cytotoxicity is a function of interferon concentration, and that two stages in the NK cell dif...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-3083.1980.tb00040.x

    authors: Senik A,Kolb JP,Orn A,Gidlund M

    更新日期:1980-01-01 00:00:00

  • The MHC class I associated beta 2-microglobulin (beta 2m) light chain is expressed in a molar excess over HLA-ABC and CD1 on the membrane of leukaemic B cells but not leukaemic T cells: evidence for further beta 2m-associated molecules.

    abstract::Beta 2-microglobulin (beta 2m) constitutes the common light chain of both the MHC-encoded HLA-ABC molecules and a group of structurally related glycoproteins recognized by antibodies of the first cluster of differentiation (CD1a, CD1b and CD1c). These CD1 antigens appear similar to murine T1 and Qa molecules in terms ...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-3083.1991.tb01520.x

    authors: Jones RA,Child JA,Master PS,Scott CS

    更新日期:1991-07-01 00:00:00

  • Induction of cellular immune reactions by A36, an antigen complex of Mycobacterium paratuberculosis: comparison of A36 and johnin components.

    abstract::Paratuberculosis (Johne's disease) is a chronic enteritis syndrome of ruminants, which is due to infection by Mycobacterium paratuberculosis. Cutaneous testing with proteins extracted from a mycobacterial culture fluid (johnin-PPD) is currently used to evaluate the cellular immune status. We have compared the componen...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-3083.1992.tb03143.x

    authors: Gilot P,De Kesel M,Coene M,Cocito C

    更新日期:1992-12-01 00:00:00

  • Downregulation of CD94/NKG2A inhibitory receptor on decreased γδ T cells in patients with systemic lupus erythematosus.

    abstract::γδ T cells are characterized by recognizing conserved endogenous and stress-induced antigens without antigen presentation. It has been show that γδ T cells play an important role in anti-tumour/microbe responses, but their function in autoimmune diseases is yet not clear. Here, we reported the quantity and phenotype o...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-3083.2012.02705.x

    authors: Wang L,Kang N,Zhou J,Guo Y,Zhang X,Cui L,Ba D,He W

    更新日期:2012-07-01 00:00:00

  • Human anti-F(ab')2 antibodies and pepsin agglutinators react with Fv determinants.

    abstract::Affinity-purified human IgG anti-tetanus antibody was subjected to papain and then pepsin digestion, and residual fragments retaining antibody activity were re-isolated by adsorption and elution from Sepharose-tetanus toxoid columns. Both Fab' and Fv fragments isolated by gel filtration showed strong reactivity with a...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-3083.1986.tb03082.x

    authors: Silvestris F,Williams RC Jr,Searles RP

    更新日期:1986-04-01 00:00:00

  • Roles of group IIA phospholipase A2 and complement in killing of bacteria by acute phase serum.

    abstract::The complement system is regarded as an important component of the innate defence system against invading bacteria. However, synergistic actions between the complement and the other components of innate immunity are incompletely known. Human group IIA phospholipase A(2) (hGIIA PLA(2)) is an effective antibacterial enz...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-3083.2005.01678.x

    authors: Grönroos JO,Salonen JH,Viander M,Nevalainen TJ,Laine VJ

    更新日期:2005-10-01 00:00:00

  • B-cell activation in duodenal mucosa after oral cholera vaccination in IgA deficient subjects with or without IgG subclass deficiency.

    abstract::Alterations in duodenal Ig-producing cells induced by two oral cholera vaccinations were studied by two-colour immunofluorescence in mucosal tissue sections from adults with selective IgA deficiency (IgAD), either with (n = 7) or without (n = 9) frequent infections, infection-prone patients with combined IgAD and IgG ...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-3083.1993.tb01714.x

    authors: Nilssen DE,Friman V,Theman K,Björkander J,Kilander A,Holmgren J,Hanson LA,Brandtzaeg P

    更新日期:1993-08-01 00:00:00

  • Monoclonal antibodies against measles virus haemagglutinin react with synthetic peptides.

    abstract::The ability of 17 monoclonal antibodies (MoAb) against measles virus haemagglutinin (MV-H) to bind to 10 selected MV-H-specific synthetic peptides was tested in an enzyme immunoassay (EIA). Three peptides representing residues 126-135 (close to the NH2 terminus), 309-318 (middle), and 587-596 (C-terminal) reacted with...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-3083.1989.tb01205.x

    authors: Mäkelä MJ,Salmi AA,Norrby E,Wild TF

    更新日期:1989-08-01 00:00:00

  • Lymphoid stem cells in the intraembryonic mesenchyme of the chicken.

    abstract::Chromosomally marked cells from the 7-day intraembryonic mesenchyme were transplanted into 14-day-old irradiated chick embryos. At the age of 6 weeks donor-derived T and B lymphocytes were shown to be present in the thymus, spleen and bone marrow, indicating that cells in the 7-day intraembryonic mesenchyme are capabl...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-3083.1980.tb00011.x

    authors: Lassila O,Eskola J,Toivanen P,Dieterlen-Lièvre F

    更新日期:1980-01-01 00:00:00

  • Properties of cell subpopulations of starfish axial organ: in vitro effect of pokeweed mitogen and evidence of lymphokine-like substances.

    abstract::The in vitro effects of pokeweed mitogen (PWM) on axial organ (AO) cells of the echinoderm starfish Asteria rubens have been studied. PWM stimulates 3H-thymidine incorporation by the whole population of AO cells (index of stimulation 4-5). On the basis of surface adherence to nylon wool the AO cell population can be f...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-3083.1981.tb00565.x

    authors: Leclerc M,Brillouet C,Luquet G,Agogue P,Binaghi RA

    更新日期:1981-09-01 00:00:00

  • The potential role of mannan-binding lectin in the clearance of self-components including immune complexes.

    abstract::Mannan-binding lectin (MBL) is a pattern recognition receptor in the innate immune system. It recognizes certain sugar residues arranged in a pattern that enables MBL to bind with sufficient strength. Such sugar patterns are common on the surface of many microorganisms, and MBL has therefore been considered to be an a...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章,评审

    doi:10.1111/j.0300-9475.2004.01437.x

    authors: Saevarsdottir S,Vikingsdottir T,Valdimarsson H

    更新日期:2004-07-01 00:00:00

  • Reactivity patterns of synovial T-cell lines derived from a patient with rheumatoid arthritis. I. Reactions with defined antigens and auto-antigens suggest the existence of multireactive T-cell clones.

    abstract::The functional T-cell repertoire in the inflamed joint of a patient with rheumatoid arthritis was analysed at the clonal level. Using limiting dilution techniques and selecting for growth of in vivo activated and/or autoreactive T cells, 149 T-cell lines were established. They were tested in a proliferation assay for ...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1046/j.1365-3083.1997.d01-105.x

    authors: Melchers I,Jooss-Rüdiger J,Peter HH

    更新日期:1997-08-01 00:00:00

  • Rabbit anti-human beta 2 microglobulin antibodies: a T-cell mitogen.

    abstract::Rabbit anti-beta 2 microglobulin antibodies have been described as a potent mitogen for human B lymphocytes. However, when fractionated after activation, only the T-cell enriched population is actively dividing. The induction of proliferation in purified T cells requires the presence of non-T cells. Daudi cells (which...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-3083.1985.tb01930.x

    authors: Hammarström L,Smith CI

    更新日期:1985-12-01 00:00:00

  • The Plasma Level of Proprotein Convertase FURIN in Patients with Suspected Infection in the Emergency Room: A Prospective Cohort Study.

    abstract::There is an increasing need for novel biomarkers that enable better diagnostic and prognostic stratification of patients with suspected infection. A proprotein convertase enzyme FURIN is upregulated upon immune cell activation, and it promotes infectivity by cleaving and activating pathogens. In this study, we determi...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/sji.12386

    authors: Ranta N,Turpeinen H,Oksanen A,Hämäläinen S,Huttunen R,Uusitalo-Seppälä R,Rintala E,Aittoniemi J,Pesu M

    更新日期:2015-12-01 00:00:00

  • Isolation and partial characterization of rat lymphoid cell surface histocompatibility antigens and immunoglobulins.

    abstract::Cell surface molecules of rat normal lymphoid cells were selectively labelled by lactoperoxidase catalysed iodination or by a galactose oxidase tritiated sodium borohydride technique, subsequently detergent solubilized, isolated by indirect immunoprecipitation and analysed on SDS-polyacrylamide gel electrophoresis. Fo...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-3083.1978.tb00458.x

    authors: Nilsson F,Wigzell H

    更新日期:1978-04-01 00:00:00

  • Inhibitory effect of haptoglobin on granulocyte chemotaxis, phagocytosis and bactericidal activity.

    abstract::Human haptoglobin (Hp) is synthesized at hepatic and extrahepatic sites as an acute-phase reactant protein (APP). We investigated the effects of Hp on granulocyte function. The chemotaxis of freshly isolated human granulocytes and differentiated HL-60 cells in response to the bacterial tripeptide, f-met-leu-phe, was i...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1046/j.1365-3083.1999.00609.x

    authors: Rossbacher J,Wagner L,Pasternack MS

    更新日期:1999-10-01 00:00:00

  • NcoI restriction fragment length polymorphism (RFLP) of the tumour necrosis factor (TNF alpha) region in primary biliary cirrhosis and in healthy Danes.

    abstract::The restriction fragment length polymorphism of the human tumour necrosis factor (TNF alpha) region was investigated by means of 20 different restriction enzymes and a human TNF alpha cDNA probe. Only one of the enzymes, NcoI, revealed a polymorphic pattern consisting of fragments of 10.5 and 5.5 kb, which behaved as ...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-3083.1989.tb01200.x

    authors: Fugger L,Morling N,Ryder LP,Platz P,Georgsen J,Jakobsen BK,Svejgaard A,Dalhoff K,Ranek L

    更新日期:1989-08-01 00:00:00

  • DC-SIGN-specific liposomal targeting and selective intracellular compound delivery to human myeloid dendritic cells: implications for HIV disease.

    abstract::Myeloid dendritic cells (MyDCs), prime stimulators of antigen-specific immunity, can serve as one of the major reservoirs for human immunodeficiency virus type-1 (HIV-1). Utilizing mature monocyte-derived MyDCs generated with granulocyte/macrophage colony-stimulating factor, interleukin-4, and tumour necrosis factor-a...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/j.0300-9475.2004.01431.x

    authors: Gieseler RK,Marquitan G,Hahn MJ,Perdon LA,Driessen WH,Sullivan SM,Scolaro MJ

    更新日期:2004-05-01 00:00:00

  • Variation in gut-homing CD27-negative lymphocytes in inflammatory colon disease.

    abstract::Prolonged antigenic stimulation results in lymphocyte shedding of CD27, a member of the tumour necrosis factor receptor (TNFR) family, and transformation to a stable phenotype capable of synthesizing interleukin-4 (IL-4). Co-expression of alpha4beta7 identifies those cells with gut-homing potential. We have investigat...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1046/j.1365-3083.1998.00387.x

    authors: Meenan J,Spaans J,Grool TA,Lammers K,Pals S,Tytgat GN,van Deventer SJ

    更新日期:1998-09-01 00:00:00

  • Measurement of IgE on rat mast cells: relation to serum IgE and allergen-induced histamine release.

    abstract::IgE antibodies against trinitrophenylated ovalbumin (TNP-OVA) were raised in Brown Norway rats by a highly reproducible immunization procedure. With these rats, the kinetics of the IgE response was studied, both for free circulating and cell-bound IgE. Cell-bound IgE was dissociated from peritoneal cell suspensions at...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-3083.1983.tb00760.x

    authors: van Toorenenbergen AW,van Swieten P,Aalberse RC

    更新日期:1983-01-01 00:00:00

  • The role of superoxide anion and lysosomal enzymes in anti-listerial activity of elicited peritoneal macrophages.

    abstract::The in vitro effect of superoxide anion and lysosomal enzyme activity on the killing of Listeria monocytogenes EGD (listeria) by peritoneal macrophages (PM) was investigated. Generation of superoxide anion by PM stimulated with phorbol myristate acetate (PMA) was significantly increased by intraperitoneal injection of...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-3083.1986.tb02131.x

    authors: Hashimoto S,Nomoto K,Yokokura T

    更新日期:1986-10-01 00:00:00

  • Peripheral blood neutrophils of germ-free rats modified by in vivo granulocyte-colony-stimulating factor and exposure to natural environment.

    abstract::Peripheral blood neutrophils from germ-free (GF), specific-pathogen-free (SPF) and conventional (CV) rats were compared. Besides neutropenia and impaired superoxide anion generation as previously reported, it was found that GF rats had lower phagocytic function (70%) and generated less nitric oxide than the other rats...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1046/j.1365-3083.1999.00456.x

    authors: Ohkubo T,Tsuda M,Suzuki S,El Borai N,Yamamura M

    更新日期:1999-01-01 00:00:00

  • Proteasomal targeting and minigene repetition improve cell-surface presentation of a transfected, modified melanoma tumour antigen.

    abstract::Melanoma antigen recognized by T cell 1 (MART-1) is regarded as a candidate peptide for vaccination against malignant melanoma, and it is of importance to develop strategies to improve the vaccine-elicited T-cell activation towards MART-1. T-cell activation is, among other determinants, dependent on the density of spe...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/j.0300-9475.2004.01374.x

    authors: Rasmussen AB,Zocca MB,Bonefeld CM,von Essen M,Lauritsen JP,Tomra S,Ødum N,Geisler C

    更新日期:2004-02-01 00:00:00

  • Interleukin-1 production by a cloned line of human monocyte-like cells (CM-SM). Correlation with state of differentiation.

    abstract::Interleukin-1 (IL-1) production by the human monocyte-like cloned cell line CM-SM has been investigated as a function of the state of cell differentiation. CM-SM cells were induced to differentiate along the monocyte-macrophage lineage by bacterial lipopolysaccharides (LPS) or by 12-O-tetradecanoyl-phorbol-13-acetate ...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-3083.1985.tb01397.x

    authors: Revoltella RP,Butler RH,Musiani P,Piantelli M,Ranelletti FO

    更新日期:1985-01-01 00:00:00

  • Blockade of the B7-CD28 pathway by CTLA4-Ig counteracts rejection and prolongs survival in small bowel transplantation.

    abstract::Allograft rejection involves T-cell activation, requiring T-cell receptor interactions with major histocompatibility complex (MHC) molecules and costimulatory signals delivered through the B7-CD28 pathway. We evaluated the effect of blocking this pathway on graft rejection and survival, in a rat experimental model of ...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1046/j.1365-3083.2000.00725.x

    authors: Kurlberg G,Haglind E,Schön K,Törnqvist H,Lycke N

    更新日期:2000-03-01 00:00:00

  • Highly increased natural killer cell number and lytic activity in the murine peripheral blood and lungs after interferon induction in vivo.

    abstract::We have studied the effect of interferon in vivo (induced by polyinosinic-polycytidylic acid (pIC] on the natural killer (NK) cell lytic activity and on the numbers of large granular lymphocytes (LGL) and target-binding cells (TBC) in different lymphoid compartments. One day after the pIC induction the spleen containe...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-3083.1984.tb01016.x

    authors: Hurme M,Silvennoinen O,Renkonen R

    更新日期:1984-10-01 00:00:00

  • Upregulation of the expression of Toll-like receptor 9 in basophils in patients with allergic rhinitis: An enhanced expression by allergens.

    abstract::It was reported that the expression of Toll-like receptor (TLR) 9 may be related to Th2-type allergic inflammation including allergic rhinitis (AR). However, little is known about the expression of TLR9 in the basophils in AR. In the present study, the expression of TLR9 was examined by flow cytometry analysis, and th...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/sji.13003

    authors: Zhong Q,Zhan M,Wang L,Chen D,Zhao N,Wang J,Huang Y,Zhang X,He S,Zhang H

    更新日期:2020-11-28 00:00:00

  • Immune balance in Hepatitis B Infection: Present and Future Therapies.

    abstract::Chronic hepatitis B virus (HBV) infection affects millions of people worldwide and about half a million people die every year. India represents the second largest pool of chronic HBV infections with an estimated 40 million chronically infected patients. Persistence or clearance of HBV infection mainly depends upon hos...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章,评审

    doi:10.1111/sji.12553

    authors: Vyas AK,Jindal A,Hissar S,Ramakrishna G,Trehanpati N

    更新日期:2017-07-01 00:00:00

  • Idiotypic multireactivity of 'natural' antibodies. 'Natural' anti-idiotypes also inhibit helper cells with cross-reactive clonotypes.

    abstract::One hundred and twenty IgM-secreting hybridomas derived from unmanipulated 6-day-old BALB/c mice were screened for reactivity with the prototype idiotypic and anti-idiotypic monoclonal antibodies, defining three well established systems, namely TEPC 15:10/13-15, J558:CD3.2, and MOPC 460:F 6(51). Up to 25% of all IgM a...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-3083.1987.tb02221.x

    authors: Araujo PM,Holmberg D,Martinez-A C,Coutinho A

    更新日期:1987-05-01 00:00:00

  • A solid-phase radioimmunoassay for C1q-binding immune complexes. I. Delta IgG as indicator molecule.

    abstract::A solid-phase radioimmunoassay for detection of circulating C1q-binding immune complexes (IC) or heat-aggregated IgG (delta IgG) is described. Purified human C1q protein was adsorbed to a fixed area of polystyrene tube surface for 1 hr at 22 degrees C, pH 7.3, mu 0.15. Binding of delta IgG or IC to solid-phase C1q at ...

    journal_title:Scandinavian journal of immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-3083.1975.tb02677.x

    authors: Svehag SE

    更新日期:1975-01-01 00:00:00