Abstract：
:CD25 monoclonal antibody binding to the alpha-chain of the Interleukin-2 (IL-2) receptor, blocks high-affinity IL-2 binding, thereby preventing complete T-cell activation and being of ample importance in transplantation medicine and potentially the treatment of autoimmune disease. However, CD25 antibodies do not only block T-cell activation but also prevent activation-induced cell death (AICD) attributing a dual function to IL-2. In this study, the modulation of the genomic expression profile of human peripheral blood mononuclear cells (PBMC) with therapeutic concentrations of humanized anti-CD25 mAb was investigated. PBMC were stimulated with CD3 antibody OKT-3 together with recombinant IL-2 in the absence or presence of anti-CD25 mAb. RNA was extracted and subjected to microarray analysis on U133A microarrays (Affymetrix). Anti-CD25 treatment inhibited several genes typically expressed during T-cell activation including granzyme B, signalling lymphocyte activation molecule, family member 1 (SLAMF1), CD40-Ligand (CD40-L), IL-9 and interferon (IFN)-gamma. Interestingly, anti-CD25 mAb also blocked the expression of several genes important for susceptibility to apoptosis, such as death receptor 6 (DR6) or reversed IL-2-mediated repression of anti-apoptotic genes, such as Fas apoptotic inhibitory molecule 3 (FAIM3)/TOSO. Functional significance of DR6 and TOSO expression in IL-2-dependent T-cell activation was subsequently evaluated by RNA interference in AICD: While siRNA specifically directed against DR6 did not modulate FAS-L-mediated apoptosis induction in primary T cells, down-regulation of TOSO significantly increased susceptibility to apoptosis, emphasizing an important role for TOSO in IL-2-mediated AICD.
journal_name
Scand J Immunoljournal_title
Scandinavian journal of immunologyauthors
Richter GH,Mollweide A,Hanewinkel K,Zobywalski C,Burdach Sdoi
10.1111/j.1365-3083.2009.02281.xsubject
Has Abstractpub_date
2009-09-01 00:00:00pages
206-15issue
3eissn
0300-9475issn
1365-3083pii
SJI2281journal_volume
70pub_type
杂志文章abstract：:Rat AgB transplantation antigens were isolated after papain digestion of spleens from the inbred strain Hooded Lister. Both subunits of the AgB antigens were present in the purified material. Some physical characteristics of the antigens have been determined. An antiserum, raised in a rabbit, against the purified mate...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1980.tb00209.x
更新日期：1980-01-01 00:00:00
abstract：:The cellular and humoral natural immune response induced by hepatitis C virus (HCV) is commonly unable to eradicate the virus. HCV is a highly mutable, hepatotropic RNA virus that causes acute and chronic hepatitis, an infection that involves the production of various cytokines. The aim of the study is to analyse the ...
journal_title：Scandinavian journal of immunology
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doi：10.1111/j.1365-3083.2006.001761.x
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更新日期：2009-11-01 00:00:00
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章,评审
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2012.02685.x
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/sji.12574
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journal_title：Scandinavian journal of immunology
pub_type： 历史文章,杂志文章
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1991.tb01557.x
更新日期：1991-09-01 00:00:00
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-3083.2010.02501.x
更新日期：2011-05-01 00:00:00
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.2003.01227.x
更新日期：2003-04-01 00:00:00
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/sji.12399
更新日期：2016-02-01 00:00:00
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2006.01774.x
更新日期：2006-07-01 00:00:00
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1988.tb02327.x
更新日期：1988-01-01 00:00:00
abstract：:A method of isolation has been developed to purify mast cells from human lung tissue. The purification steps are: (1) dispersion of human lung tissue in single-cell suspensions by enzymatic digestion, (2) partial purification by counterflow centrifugal elutriation, (3) Percoll gradient centrifugation, and (4) enrichme...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1988.tb02316.x
更新日期：1988-01-01 00:00:00
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-3083.2007.01949.x
更新日期：2007-07-01 00:00:00
abstract：:We report here the molecular characterization of the T-cell receptor (TCR) expressed by a human HLA-A2 specific cytotoxic T-cell clone named CTL 49. Flow cytometry analysis with a panel of anti-TCR antibodies revealed an OKT3+, WT31+, A13+, BB3-, TCR delta-, delta TCS1-, TCR gamma/delta 1-, OKT4-, and OKT8+ phenotype,...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1992.tb02883.x
更新日期：1992-04-01 00:00:00
abstract：:World Health Organisation recommends the practice of BCG vaccination at birth in countries which have a high incidence of tuberculosis and/or high leprosy burden. The BCG vaccination is considered safe for a competent immune system. However, in children with weakened immune systems cause of which can be primary or sec...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/sji.13010
更新日期：2020-12-16 00:00:00
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1993.tb01708.x
更新日期：1993-08-01 00:00:00
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journal_title：Scandinavian journal of immunology
pub_type： 历史文章,杂志文章,评审
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更新日期：2001-04-01 00:00:00
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
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更新日期：1999-12-01 00:00:00
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1989.tb02456.x
更新日期：1989-10-01 00:00:00
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.2000.00690.x
更新日期：2000-04-01 00:00:00
abstract：:ESAT-6 is a protein produced by virulent Mycobacterium tuberculosis but absent in Mycobacterium bovis bacillus Calmette-Guerin (BCG) and is considered as a promising vaccine subunit against tuberculosis. The protective efficacy of a new strategy based on the combination of DNA vaccine encoding ESAT-6 (DNA-E6) and BCG ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2007.02006.x
更新日期：2007-11-01 00:00:00
abstract：:T cells from immune guinea-pigs produce clusters in vitro with macrophages pulsed with soluble protein antigens. The formation of clusters is antigen-specific. Time-lapse cinephotomicrography was used to study the sequence of events leading to cluster formation and the kinetics of already formed clusters. The central ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1979.tb01380.x
更新日期：1979-01-01 00:00:00
abstract：:Lewis rats develop arthritis after immunization with heterologous but not homologous rat type II collagen (CII). We have observed that if the rat CII is prepared by pepsin digestion without subsequent extensive purification, it is arthritogenic in Lewis rats. To address whether pepsin in the CII preparations contribut...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1993.tb02562.x
更新日期：1993-03-01 00:00:00
abstract：:Human peripheral lymphocytes were cultivated in soft agar or agarose, containing 3H-thymidine or bromodeoxyuridine (BrdU), which is an analogue of thymidine readily incorporated into deoxyribonucleic acid by lymphocytes. Colonies were isolated and analysed by autoradiography or stained with the fluorescence-plus-Giems...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1982.tb00680.x
更新日期：1981-05-01 00:00:00
abstract：:We have studied the interaction of mitogenic lectins such as phytohaemagglutinin (PHA) and concanavalin A (Con A) with both surface molecules which, by the use of monoclonal antibodies, are known to trigger T-cell mitogenesis. Monoclonal antibodies recognizing the T-lymphocyte receptor for antigen (Ti) and/or its asso...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1986.tb01985.x
更新日期：1986-05-01 00:00:00
abstract：:Paracoccidioidomycosis (PCM) is a systemic mycosis caused by Paracoccidiodes brasiliensis that presents a wide spectrum of clinical manifestations. Because of the great number of neutrophils polymorphonuclear neutrophils (PMN) found in the P. brasiliensis granuloma, studies have been done to evaluate the role of these...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
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更新日期：2009-02-01 00:00:00
abstract：:Multifactorial involvement in the pathogenesis of autoimmune NZB/W F1 mice has been well documented. To further elucidate the role of cytokines in the disease development of murine lupus, single spleen cells isolated from NZB/W F1 and non-autoimmune C57BL/6 mice were stimulated with T cell mitogens or anti-CD3 antibod...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1995.tb03681.x
更新日期：1995-10-01 00:00:00
abstract：:This paper briefly reviews commonly used procedures for separation of mononuclear cells (MNC) and granulocytes from human blood with X-ray contrast media as gradient material, and also presents new and modified procedures for leucocyte preparation. Standard techniques for human blood do not always yield satisfactory r...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1991.tb01594.x
更新日期：1991-12-01 00:00:00
