Abstract：
:Aortic stenosis (AS) is the most common form of valve disease. Once symptoms develop, there is an inexorable deterioration with a poor prognosis; currently there are no therapies capable of modifying disease progression, and aortic valve replacement is the only available treatment. Our goal is to study the progression of calcification by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) and get new insights at molecular level that could help in the understanding of this disease. In this work, we analyzed consecutive slices from aortic valve tissue by MALDI-IMS, to establish the spatial distribution of proteins and peptides directly from the surface of the histological sections. The analysis showed different structures corresponding to regions observed in conventional histology, including large calcification areas and zones rich in collagen and elastic fibers. Peptide extraction from the tissue, followed by liquid chromatography mass spectrometry analysis, provided the identification of collagen VI α-3 and NDRG2 proteins which correlated with the masses obtained by MALDI-IMS and were confirmed by immunohistochemistry. These results highlighted the molecular mechanism implied in AS using MALDI-IMS, a novel technique never used before in this pathology. In addition, we can define specific regions proving a complementary resolution of the molecular histology.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Mourino-Alvarez L,Iloro I,de la Cuesta F,Azkargorta M,Sastre-Oliva T,Escobes I,Lopez-Almodovar LF,Sanchez PL,Urreta H,Fernandez-Aviles F,Pinto A,Padial LR,Akerström F,Elortza F,Barderas MGdoi
10.1038/srep27106subject
Has Abstractpub_date
2016-06-03 00:00:00pages
27106issn
2045-2322pii
srep27106journal_volume
6pub_type
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