Conformational transitions induced by γ-amino butyrate binding in GabR, a bacterial transcriptional regulator.

Abstract:

:GabR from Bacillus subtilis is a transcriptional regulator of the MocR subfamily of GntR regulators. The MocR architecture is characterized by the presence of an N-terminal winged-Helix-Turn-Helix domain and a C-terminal domain folded as the pyridoxal 5'-phosphate (PLP) dependent aspartate aminotransferase (AAT). The two domains are linked by a peptide bridge. GabR activates transcription of genes involved in γ-amino butyrate (GABA) degradation upon binding of PLP and GABA. This work is aimed at contributing to the understanding of the molecular mechanism underlying the GabR transcription activation upon GABA binding. To this purpose, the structure of the entire GabR dimer with GABA external aldimine (holo-GABA) has been reconstructed using available crystallographic data. The structure of the apo (without any ligand) and holo (with PLP) GabR forms have been derived from the holo-GABA. An extensive 1 μs comparative molecular dynamics (MD) has been applied to the three forms. Results showed that the presence of GABA external aldimine stiffens the GabR, stabilizes the AAT domain in the closed form and couples the AAT and HTH domains dynamics. Apo and holo GabR appear more flexible especially at the level of the HTH and linker portions and small AAT subdomain.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Frezzini M,Guidoni L,Pascarella S

doi

10.1038/s41598-019-55581-1

subject

Has Abstract

pub_date

2019-12-17 00:00:00

pages

19319

issue

1

issn

2045-2322

pii

10.1038/s41598-019-55581-1

journal_volume

9

pub_type

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