Abstract：
:The TMPRSS2:ERG gene fusion is one of a series of highly promising prostate cancer (PCa) biomarker alternatives to the controversial serum PSA. Current methods for detecting TMPRSS2:ERG are limited in terms of long processing time, high cost and the need for specialized equipment. Thus, there is an unmet need for less complex, faster, and cheaper methods to enable gene fusion detection in the clinic. We describe herein a simple, rapid and inexpensive assay which combines robust isothermal amplification technique with a novel visualization method for evaluating urinary TMPRSS2:ERG status at less than USD 5 and with minimal equipment. The assay is sensitive, and rapidly detects as low as 10(5) copies of TMPRSS2:ERG transcripts while maintaining high levels of specificity.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Koo KM,Wee EJ,Mainwaring PN,Trau Mdoi
10.1038/srep30722subject
Has Abstractpub_date
2016-07-29 00:00:00pages
30722issn
2045-2322pii
srep30722journal_volume
6pub_type
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