Mammary fibroblasts reduce apoptosis and speed estrogen-induced hyperplasia in an organotypic MCF7-derived duct model.

Abstract:

:The estrogen receptor (ER) regulates the survival and growth of breast cancer cells, but it is less clear how components of the tissue microenvironment affect ER-mediated responses. We set out to test how human mammary fibroblasts (HMFs) modulate ER signaling and downstream cellular responses. We exposed an organotypic mammary model consisting of a collagen-embedded duct structure lined with MCF7 cells to 17-β estradiol (E2), with and without HMFs in the surrounding matrix. MCF7 cells grown as ductal structures were polarized and proliferated at rates comparable to in vivo breast tissue. In both culture platforms, exposure to E2 increased ER transactivation, increased proliferation, and induced ductal hyperplasia. When the surrounding matrix contained HMFs, the onset and severity of E2-induced ductal hyperplasia was increased due to decreased apoptosis. The reduced apoptosis may be due to fibroblasts modulating ER signaling in MCF7 cells, as suggested by the increased ER transactivation and reduced ER protein in MCF7 cells grown in co-culture. These findings demonstrate the utility of organotypic platforms when studying stromal:epithelial interactions, and add to existing literature that implicate the mammary microenvironment in ER + breast cancer progression.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Morgan MM,Livingston MK,Warrick JW,Stanek EM,Alarid ET,Beebe DJ,Johnson BP

doi

10.1038/s41598-018-25461-1

subject

Has Abstract

pub_date

2018-05-08 00:00:00

pages

7139

issue

1

issn

2045-2322

pii

10.1038/s41598-018-25461-1

journal_volume

8

pub_type

杂志文章
  • Large diameter scleral lens benefits for Asians with intractable ocular surface diseases: a prospective, single-arm clinical trial.

    abstract::To report the efficacy and safety of large diameter scleral lenses and determine their suitability in Asian subjects with intractable ocular surface diseases. This prospective study enrolled intractable ocular surface diseases subjects with uncorrected visual acuity > counting finger but ≥ 0.3 logMAR and best-correcte...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-021-82010-z

    authors: Moon J,Lee SM,Hyon JY,Kim MK,Oh JY,Choi HJ

    更新日期:2021-01-27 00:00:00

  • Tmc2 expression partially restores auditory function in a mouse model of DFNB7/B11 deafness caused by loss of Tmc1 function.

    abstract::Mouse Tmc1 and Tmc2 are required for sensory transduction in cochlear and vestibular hair cells. Homozygous Tmc1∆/∆ mice are deaf, Tmc2∆/∆ mice have normal hearing, and double homozygous Tmc1∆/∆; Tmc2∆/∆ mice have deafness and profound vestibular dysfunction. These phenotypes are consistent with their different spatio...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-29709-8

    authors: Nakanishi H,Kurima K,Pan B,Wangemann P,Fitzgerald TS,Géléoc GS,Holt JR,Griffith AJ

    更新日期:2018-08-14 00:00:00

  • A unique clade of light-driven proton-pumping rhodopsins evolved in the cyanobacterial lineage.

    abstract::Microbial rhodopsin is a photoreceptor protein found in various bacteria and archaea, and it is considered to be a light-utilization device unique to heterotrophs. Recent studies have shown that several cyanobacterial genomes also include genes that encode rhodopsins, indicating that these auxiliary light-utilizing pr...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-73606-y

    authors: Hasegawa M,Hosaka T,Kojima K,Nishimura Y,Nakajima Y,Kimura-Someya T,Shirouzu M,Sudo Y,Yoshizawa S

    更新日期:2020-10-07 00:00:00

  • Synthesis and characterization of CaSr-Metal Organic Frameworks for biodegradable orthopedic applications.

    abstract::Metal-organic frameworks (MOFs) formed from metals and organic ligands, are crystalline materials that are degradable in aqueous medium, and capable of releasing Ca and Sr ions. In this manuscript, the ability of MOFs to degrade and release osteogenic Ca and Sr ions was investigated. MOFs were generated by choosing os...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-49536-9

    authors: Joseph N,Lawson HD,Overholt KJ,Damodaran K,Gottardi R,Acharya AP,Little SR

    更新日期:2019-09-10 00:00:00

  • Novel common variants and susceptible haplotype for exfoliation glaucoma specific to Asian population.

    abstract::The common variants in lysyl oxidase-like 1 gene (LOXL1) are associated with exfoliation glaucoma (XFG) patients developed through exfoliation syndrome (XFS). However, the risk allele of a variant in LOXL1 has been found to be inverted between Asian and Caucasian populations. Therefore, we newly performed a genome-wid...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep05340

    authors: Nakano M,Ikeda Y,Tokuda Y,Fuwa M,Ueno M,Imai K,Sato R,Omi N,Adachi H,Kageyama M,Mori K,Kinoshita S,Tashiro K

    更新日期:2014-06-18 00:00:00

  • Rating knockdown of flour beetles after exposure to two insecticides as an indicator of mortality.

    abstract::Knockdown and mortality of adults of the red flour beetle, Tribolium castaneum (Herbst) and the confused flour beetle, Tribolium confusum Jacquelin du Val, were assessed after exposure to two contact insecticides, chlorfenapyr and cyfluthrin, on a concrete surface. Individuals were rated on a scale for knockdown of ex...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-78982-z

    authors: Athanassiou CG,Kavallieratos NG,Arthur FH,Nakas CT

    更新日期:2021-01-13 00:00:00

  • Generation of light with controllable spatial patterns via the sum frequency in quasi-phase matching crystals.

    abstract::Light beams with extraordinary spatial structures, such as the Airy beam (AB), the Bessel-Gaussian beam (BGB) and the Laguerre-Gaussian beam (LGB), are widely studied and applied in many optical scenarios. We report on preparation of light beams with controllable spatial structures through sum frequency generation (SF...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep05650

    authors: Zhou ZY,Li Y,Ding DS,Jiang YK,Zhang W,Shi S,Shi BS,Guo GC

    更新日期:2014-07-10 00:00:00

  • Evaluating the performance of raw and epoch non-wear algorithms using multiple accelerometers and electrocardiogram recordings.

    abstract::Accurate detection of accelerometer non-wear time is crucial for calculating physical activity summary statistics. In this study, we evaluated three epoch-based non-wear algorithms (Hecht, Troiano, and Choi) and one raw-based algorithm (Hees). In addition, we performed a sensitivity analysis to provide insight into th...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-62821-2

    authors: Syed S,Morseth B,Hopstock LA,Horsch A

    更新日期:2020-04-03 00:00:00

  • Retaining information from multidimensional correlation MRI using a spectral regions of interest generator.

    abstract::Multidimensional correlation magnetic resonance imaging (MRI) is an emerging imaging modality that is capable of disentangling highly heterogeneous and opaque systems according to chemical and physical interactions of water within them. Using this approach, the conventional three dimensional MR scalar images are repla...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-60092-5

    authors: Pas K,Komlosh ME,Perl DP,Basser PJ,Benjamini D

    更新日期:2020-02-24 00:00:00

  • A Joint Modeling Approach for Childhood Meat, Fish and Egg Consumption and the Risk of Advanced Islet Autoimmunity.

    abstract::Several dietary factors have been suspected to play a role in the development of advanced islet autoimmunity (IA) and/or type 1 diabetes (T1D), but the evidence is fragmentary. A prospective population-based cohort of 6081 Finnish newborn infants with HLA-DQB1-conferred susceptibility to T1D was followed up to 15 year...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-44196-1

    authors: Syrjälä E,Nevalainen J,Peltonen J,Takkinen HM,Hakola L,Åkerlund M,Veijola R,Ilonen J,Toppari J,Knip M,Virtanen SM

    更新日期:2019-05-23 00:00:00

  • Soft and broadband infrared metamaterial absorber based on gold nanorod/liquid crystal hybrid with tunable total absorption.

    abstract::We design a soft infrared metamaterial absorber based on gold nanorods dispersed in liquid crystal (LC) placed on a gold film and theoretically investigate its total absorption character. Because the nanorods align with the LC molecule, the gold nanorods/LC hybrid exhibits different permittivity as a function of tilt ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep16698

    authors: Su Z,Yin J,Zhao X

    更新日期:2015-11-18 00:00:00

  • Mechanism of the Escherichia coli MltE lytic transglycosylase, the cell-wall-penetrating enzyme for Type VI secretion system assembly.

    abstract::Lytic transglycosylases (LTs) catalyze the non-hydrolytic cleavage of the bacterial cell wall by an intramolecular transacetalization reaction. This reaction is critically and broadly important in modifications of the bacterial cell wall in the course of its biosynthesis, recycling, manifestation of virulence, inserti...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-22527-y

    authors: Byun B,Mahasenan KV,Dik DA,Marous DR,Speri E,Kumarasiri M,Fisher JF,Hermoso JA,Mobashery S

    更新日期:2018-03-07 00:00:00

  • Polymorphism A118G of opioid receptor mu 1 (OPRM1) is associated with emergence of suicidal ideation at antidepressant onset in a large naturalistic cohort of depressed outpatients.

    abstract::Antidepressants have been the object of an international controversy for about thirty years. Some patients are inclined to develop suicidal ideation (SI) at antidepressant onset; this phenomenon is known as Treatment Emergent Suicidal Ideation (TESI), and it has conducted regulatory bodies to prompt warnings on antide...

    journal_title:Scientific reports

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1038/s41598-019-39622-3

    authors: Nobile B,Ramoz N,Jaussent I,Gorwood P,Olié E,Castroman JL,Guillaume S,Courtet P

    更新日期:2019-02-22 00:00:00

  • Metabolic Patterning on a Chip: Towards in vitro Liver Zonation of Primary Rat and Human Hepatocytes.

    abstract::An important number of healthy and diseased tissues shows spatial variations in their metabolic capacities across the tissue. The liver is a prime example of such heterogeneity where the gradual changes in various metabolic activities across the liver sinusoid is termed as "zonation" of the liver. Here, we introduce t...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-27179-6

    authors: Kang YBA,Eo J,Mert S,Yarmush ML,Usta OB

    更新日期:2018-06-12 00:00:00

  • Development of an Injectable Slow-Release Metformin Formulation and Evaluation of Its Potential Antitumor Effects.

    abstract::Metformin is an antidiabetic drug which possesses antiproliferative activity in cancer cells when administered at high doses, due to its unfavorable pharmacokinetics. The aim of this work was to develop a pharmacological tool for the release of metformin in proximity of the tumor, allowing high local concentrations, a...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-22054-w

    authors: Baldassari S,Solari A,Zuccari G,Drava G,Pastorino S,Fucile C,Marini V,Daga A,Pattarozzi A,Ratto A,Ferrari A,Mattioli F,Barbieri F,Caviglioli G,Florio T

    更新日期:2018-03-02 00:00:00

  • System-specific periodicity in quantitative real-time polymerase chain reaction data questions threshold-based quantitation.

    abstract::Real-time quantitative polymerase chain reaction (qPCR) data are found to display periodic patterns in the fluorescence intensity as a function of sample number for fixed cycle number. This behavior is seen for technical replicate datasets recorded on several different commercial instruments; it occurs in the baseline...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep38951

    authors: Spiess AN,Rödiger S,Burdukiewicz M,Volksdorf T,Tellinghuisen J

    更新日期:2016-12-13 00:00:00

  • Control strategies of 3-cell Central Pattern Generator via global stimuli.

    abstract::The study of the synchronization patterns of small neuron networks that control several biological processes has become an interesting growing discipline. Some of these synchronization patterns of individual neurons are related to some undesirable neurological diseases, and they are believed to play a crucial role in ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep23622

    authors: Lozano Á,Rodríguez M,Barrio R

    更新日期:2016-03-29 00:00:00

  • Designing novel bulk metallic glass composites with a high aluminum content.

    abstract::The long-standing challenge for forming Al-based BMGs and their matrix composites with a critical size larger than 1 mm have not been answered over the past three decades. In this paper, we reported formation of a series of BMG matrix composites which contain a high Al content up to 55 at.%. These composites can be ca...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep03353

    authors: Chen ZP,Gao JE,Wu Y,Wang H,Liu XJ,Lu ZP

    更新日期:2013-11-27 00:00:00

  • Individual and population-level responses to ocean acidification.

    abstract::Ocean acidification is predicted to have detrimental effects on many marine organisms and ecological processes. Despite growing evidence for direct impacts on specific species, few studies have simultaneously considered the effects of ocean acidification on individuals (e.g. consequences for energy budgets and resourc...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep20194

    authors: Harvey BP,McKeown NJ,Rastrick SP,Bertolini C,Foggo A,Graham H,Hall-Spencer JM,Milazzo M,Shaw PW,Small DP,Moore PJ

    更新日期:2016-01-29 00:00:00

  • Transcriptional repressor domain of MBD1 is intrinsically disordered and interacts with its binding partners in a selective manner.

    abstract::Methylation of DNA CpG sites is a major mechanism of epigenetic gene silencing and plays important roles in cell division, development and carcinogenesis. One of its regulators is the 64-residue C-terminal Transcriptional Repressor Domain (the TRD) of MBD1, which recruits several repressor proteins such as MCAF1, HDAC...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep04896

    authors: Hameed UF,Lim J,Zhang Q,Wasik MA,Yang D,Swaminathan K

    更新日期:2014-05-09 00:00:00

  • Identification of human glycosyltransferase genes expressed in erythroid cells predicts potential carbohydrate blood group loci.

    abstract::Glycans are biologically important structures synthesised by glycosyltransferase (GT) enzymes. Disruptive genetic null variants in GT genes can lead to serious illness but benign phenotypes are also seen, including antigenic differences on the red blood cell (RBC) surface, giving rise to blood groups. To characterise ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-24445-5

    authors: Jöud M,Möller M,Olsson ML

    更新日期:2018-04-16 00:00:00

  • In silico investigation of a KCNQ1 mutation associated with short QT syndrome.

    abstract::Short QT syndrome (SQTS) is a rare condition characterized by abnormally 'short' QT intervals on the ECG and increased susceptibility to cardiac arrhythmias and sudden death. This simulation study investigated arrhythmia dynamics in multi-scale human ventricle models associated with the SQT2-related V307L KCNQ1 'gain-...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-08367-2

    authors: Adeniran I,Whittaker DG,El Harchi A,Hancox JC,Zhang H

    更新日期:2017-08-16 00:00:00

  • Assessing the effects of Cry1C rice and Cry2A rice to Pseudogonatopus flavifemur, a parasitoid of rice planthoppers.

    abstract::Transgenic rice producing insecticidal proteins from Bacillus thuringiensis (Bt) could help protect the plants from damage by lepidopteran pests. However, one concern is the potential of Bt rice to harm non-target natural enemies, which play a vital role in pest control. In the present study, the potential effects of ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-08173-w

    authors: Tian JC,Romeis J,Liu K,Zhang FC,Zheng XS,Xu HX,Chen GH,He XC,Lu ZX

    更新日期:2017-08-10 00:00:00

  • A CRISPR-based method for testing the essentiality of a gene.

    abstract::The CRISPR/Cas9 system is a powerful method of editing genes by randomly introducing errors into the target sites. Here, we describe a CRISPR-based test for gene essentiality (CRISPR-E test) that allows the identification of essential genes. Specifically, we use sgRNA-mediated CRISPR/Cas9 to target the open reading fr...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-71690-8

    authors: You Y,Ramachandra SG,Jin T

    更新日期:2020-09-08 00:00:00

  • Isolation and characterization of nutrient dependent pyocyanin from Pseudomonas aeruginosa and its dye and agrochemical properties.

    abstract::Pyocyanin is a blue green phenazine pigment produced in large quantities by active cultures of Pseudomonas aeruginosa, with advantageous applications in medicine, agriculture and for the environment. Hence, in the present study, a potent bacterium was isolated from agricultural soil and was identified morphologically ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-58335-6

    authors: DeBritto S,Gajbar TD,Satapute P,Sundaram L,Lakshmikantha RY,Jogaiah S,Ito SI

    更新日期:2020-01-31 00:00:00

  • Halotolerant aminopeptidase M29 from Mesorhizobium SEMIA 3007 with biotechnological potential and its impact on biofilm synthesis.

    abstract::The aminopeptidase gene from Mesorhizobium SEMIA3007 was cloned and overexpressed in Escherichia coli. The enzyme called MesoAmp exhibited optimum activity at pH 8.5 and 45 °C and was strongly activated by Co2+ and Mn2+. Under these reaction conditions, the enzyme displayed Km and kcat values of 0.2364 ± 0.018 mM and ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-10932-8

    authors: Sierra EM,Pereira MR,Maester TC,Gomes-Pepe ES,Mendoza ER,Lemos EGM

    更新日期:2017-09-06 00:00:00

  • Molecular characterization of pyridoxine 5'-phosphate oxidase and its pathogenic forms associated with neonatal epileptic encephalopathy.

    abstract::Defects of vitamin B6 metabolism are responsible for severe neurological disorders, such as pyridoxamine 5'-phosphate oxidase deficiency (PNPOD; OMIM: 610090), an autosomal recessive inborn error of metabolism that usually manifests with neonatal-onset severe seizures and subsequent encephalopathy. At present, 27 path...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-70598-7

    authors: Barile A,Nogués I,di Salvo ML,Bunik V,Contestabile R,Tramonti A

    更新日期:2020-08-12 00:00:00

  • Dopant size effects on novel functionalities: High-temperature interfacial superconductivity.

    abstract::Among the range of complex interactions, especially at the interfaces of epitaxial oxide systems, contributing to the occurrence of intriguing effects, a predominant role is played by the local structural parameters. In this study, oxide molecular beam epitaxy grown lanthanum cuprate-based bilayers (consisting of a me...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-00539-4

    authors: Suyolcu YE,Wang Y,Baiutti F,Al-Temimy A,Gregori G,Cristiani G,Sigle W,Maier J,van Aken PA,Logvenov G

    更新日期:2017-03-28 00:00:00

  • Predicting global invasion risks: a management tool to prevent future introductions.

    abstract::Predicting regions at risk from introductions of non-native species and the subsequent invasions is a fundamental aspect of horizon scanning activities that enable the development of more effective preventative actions and planning of management measures. The Asian cyprinid fish topmouth gudgeon Pseudorasbora parva ha...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep26316

    authors: Fletcher DH,Gillingham PK,Britton JR,Blanchet S,Gozlan RE

    更新日期:2016-05-20 00:00:00

  • Point-dipole approximation for small systems of strongly coupled radiating nanorods.

    abstract::Systems of closely-spaced resonators can be strongly coupled by interactions mediated by scattered electromagnetic fields. In large systems the resulting response has been shown to be more sensitive to these collective interactions than to the detailed structure of individual resonators. Attempts to describe such syst...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-41327-6

    authors: Watson DW,Jenkins SD,Fedotov VA,Ruostekoski J

    更新日期:2019-04-05 00:00:00