Abstract:
:Nano-sized hydroxyapatite (n-HA) is considered as a bio-active material, which is often mixed into bone implant material, polyetheretherketone (PEEK). To reveal the global protein expression modulations of osteoblast in response to direct contact with the PEEK composite containing high level (40%) nano-sized hydroxyapatite (n-HA/PEEK) and explain its comprehensive bio-effects, quantitative proteomic analysis was conducted on human osteoblast-like cells MG-63 cultured on n-HA/PEEK in comparison with pure PEEK. Results from quantitative proteomic analysis showed that the most enriched categories in the up-regulated proteins were related to calcium ion processes and associated functions while the most enriched categories in the down-regulated proteins were related to RNA process. This enhanced our understanding to the molecular mechanism of the promotion of the cell adhesion and differentiation with the inhibition of the cell proliferation on n-HA/PEEK composite. It also exhibited that although the calcium ion level of incubate environment hadn't increased, merely the calcium fixed on the surface of material had influence to intracellular calcium related processes, which was also reflect by the higher intracellular Ca(2+) concentration of n-HA/PEEK. This study could lead to more comprehensive cognition to the versatile biocompatibility of composite materials. It further proves that proteomics is useful in new bio-effect discovery.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Zhao M,Li H,Liu X,Wei J,Ji J,Yang S,Hu Z,Wei Sdoi
10.1038/srep22832subject
Has Abstractpub_date
2016-03-09 00:00:00pages
22832issn
2045-2322pii
srep22832journal_volume
6pub_type
杂志文章abstract::Methylated non-CpGs (mCpH; H means A, C, and T) have emerged as key epigenetic marks in mammalian embryonic stem cells (ESCs) and neurons, regulating cell type-specific functions. In these two cell types, mCpHs show distinct motifs and correlations to transcription that could be a key in understanding the cell type-sp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11800-1
更新日期:2017-09-12 00:00:00
abstract::Age of complementary foods introduction is associated with childhood anemia, but the ideal age for the introduction of complementary foods to infants is a continuing topic of debate. We examined the longitudinal association between complementary foods introduction age and risk of anemia in 18,446 children from the Jia...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44726
更新日期:2017-03-23 00:00:00
abstract::Elevated levels of Creatine Kinase-MB (CK-MB) Isoenzyme are a common phenomenon among rotavirus (RV) diarrhea. However, few studies have addressed this issue using large sample size. In current study, 1,118 children (age <5 years) hospitalized with diarrhea in Guangzhou Women and Children's Medical Center from 2012 to...
journal_title:Scientific reports
pub_type: 历史文章,杂志文章
doi:10.1038/s41598-017-07636-4
更新日期:2017-08-09 00:00:00
abstract::To discover epigenetic changes that may underly neuroblastoma pathogenesis, we identified differentially methylated genes in neuroblastoma cells compared to neural crest cells, the presumptive precursors cells for neuroblastoma, by using genome-wide DNA methylation analysis. We previously described genes that were hyp...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55382-6
更新日期:2019-12-12 00:00:00
abstract::Given new distribution patterns of the endangered North Atlantic right whale (NARW; Eubalaena glacialis) population in recent years, an improved understanding of spatio-temporal movements are imperative for the conservation of this species. While so far visual data have provided most information on NARW movements, pas...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13359-3
更新日期:2017-10-18 00:00:00
abstract::Spotted seal (Phoca largha) is a critically endangered pinniped in China and South Korea. The conventional method to protect and maintain the P. largha population is to keep them captive in artificially controlled environments. However, little is known about the physiological differences between wild and captive P. la...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-75759-2
更新日期:2020-10-30 00:00:00
abstract::Spitzoid neoplasms constitute a morphologically distinct category of melanocytic tumors, encompassing Spitz nevus (benign), atypical Spitz tumor (intermediate malignant potential), and spitzoid melanoma (fully malignant). Currently, no reliable histopathological criteria or molecular marker is known to distinguish bor...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11200
更新日期:2015-06-10 00:00:00
abstract::The anisotropic hole and electron mobilities in N,N'-3,4,9,10-perylenediimide-1,7-phenoxy (PDIB-2OPh) and N,N'-3,4,9,10-perylenediimide (PDIB) were theoretically predicted using the Marcus-Hush theory. The substituent effect of phenoxy on their mobility rates, absorption spectra, electron affinities, and ionization po...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35555
更新日期:2016-10-19 00:00:00
abstract::Temporal precision is a determinant of performance in various motor activities. Although the accuracy and precision of timing in activities have been previously measured and quantified, temporal dynamics with flexible precision have not been considered. Here, we examined the temporal dynamics in timed motor activities...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76953-y
更新日期:2020-11-18 00:00:00
abstract::The objective of our study is to highlight the therapeutic effect and mechanism of action by which purified Flaxseed hydrolysate (PFH) which is a lignan rich fraction exerts its anticancer activity on a human breast cancer cell line (T47D) and in mice bearing tumor. HPLC analysis of PFH of six flaxseed cultivars had s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-18944-0
更新日期:2018-01-11 00:00:00
abstract::We present a proposal to realize the quantum Zeno effect (QZE) and quantum Zeno-like effect (QZLE) in a proximal (13)C nuclear spin by controlling a proximal electron spin of a nitrogen vacancy (NV) center. The measurement is performed by applying a microwave pulse to induce the transition between different electronic...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17615
更新日期:2015-12-01 00:00:00
abstract::A remarkable feature of vanadium dioxide is that it can be synthesized in a number of polymorphs. The conductivity mechanism in the metastable layered polymorph VO2(B) thin films has been investigated by terahertz time-domain spectroscopy (THz-TDS). In VO2(B), a critical temperature of 240 K marks the appearance of a ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep09182
更新日期:2015-03-17 00:00:00
abstract::Link prediction in complex networks aims at predicting the missing links from available datasets which are always incomplete and subject to interfering noises. To obtain high prediction accuracy one should try to complete the missing information and at the same time eliminate the interfering noise from the datasets. G...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14847-2
更新日期:2017-11-07 00:00:00
abstract::Chronic consumption of excess ethanol increases the risk of colorectal cancer. The pathogenesis of ethanol-related colorectal cancer (ER-CRC) is thought to be partly mediated by gut microbes. Specifically, bacteria in the colon and rectum convert ethanol to acetaldehyde (AcH), which is carcinogenic. However, the effec...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27923
更新日期:2016-06-13 00:00:00
abstract::Spontaneous preterm birth (sPTB, delivery <37 weeks gestation), accounts for approximately 10% of births worldwide; the aetiology is multifactorial with intra-amniotic infection being one contributing factor. This study aimed to determine whether asymptomatic women with a history of sPTB or cervical surgery have alter...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47756-7
更新日期:2019-08-02 00:00:00
abstract::The voltage-gated sodium channel isoform NaV1.7 is highly expressed in dorsal root ganglion neurons and is obligatory for nociceptive signal transmission. Genetic gain-of-function and loss-of-function NaV1.7 mutations have been identified in select individuals, and are associated with episodic extreme pain disorders a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71135-2
更新日期:2020-09-09 00:00:00
abstract::The Saccharomyces cerevisiae kinesin-5 Cin8 performs essential mitotic functions in spindle assembly and anaphase B spindle elongation. Recent work has shown that Cin8 is a bi-directional motor which moves towards the minus-end of microtubules (MTs) under high ionic strength (IS) conditions and changes directionality ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25597
更新日期:2016-05-24 00:00:00
abstract::Although various function of chemerin have been suggested, its physiological role remains to be elucidated. Here we show that chemerin-deficient mice are glucose intolerant irrespective of exhibiting reduced macrophage accumulation in adipose tissue. The glucose intolerance was mainly due to increased hepatic glucose ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00123
更新日期:2011-01-01 00:00:00
abstract::Identification of different isomer structures of atomic and molecular clusters has long been a challenging task in the field of cluster science. Here we present a three-dimensional (3D) assignment method, combining the energy (1D) and simulated (2D) spectra to assure the assignment of the global minimum structure. Thi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17738
更新日期:2015-12-03 00:00:00
abstract::Monosodium Glutamate (MSG) is used as flavour enhancer, with potential beneficial effects due to its nutritional value. Given the decline in kidney functions during aging, we investigated the impact of MSG voluntary intake on the kidney of male mice, aged 6 or 18 months. For 2 months, they freely consumed water (contr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80048-z
更新日期:2021-01-12 00:00:00
abstract::We report the phonon and magnetic properties of various well-stabilized Co3O4 nanoparticles. The net valence in cobalt (II)/(III) cation can be obtained by subtracting the Co2+ ions in tetrahedral interstices and Co3+ ions in the octahedral interstices, respectively, which will possess spatial inhomogeneity of its mag...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-18563-9
更新日期:2018-01-10 00:00:00
abstract::Apolipoprotein E4 (E4) and type 2 diabetes are major risk factors for cognitive decline and late onset Alzheimer's disease (AD). E4-associated phenotypes and insulin resistance (IR) share several features and appear to interact in driving cognitive dysfunction. However, shared mechanisms that could explain their overl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep43701
更新日期:2017-03-08 00:00:00
abstract::In this paper, we investigate the possibility of improving efficiency in non-radiative wireless power transfer (WPT) using metasurfaces embedded between two current varying coils and present a complete theoretical analysis of this system. We use a point-dipole approximation to calculate the fields of the coils. Based ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33174-8
更新日期:2018-10-05 00:00:00
abstract::This research investigated the association between prolonged disposable diaper (DD) wearing in infancy and primary enuresis (PNE). As a case-control study, we collected data from 376 children with enuresis and 379 healthy children who were sex- and age-matched at three tertiary care institutions in mainland China from...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-70195-8
更新日期:2020-09-01 00:00:00
abstract::Rhegmatogenous retinal detachment (RRD) is an ophthalmic emergency, which usually requires prompt surgery to prevent further detachment and restore sensory function. Although several individual factors have been suggested, a systems level understanding of molecular pathomechanisms underlying this severe eye disorder i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80005-w
更新日期:2021-01-13 00:00:00
abstract::A histoanatomical context is imperative in an analysis of gene expression in a cell in a tissue to elucidate physiological function of the cell. In this study, we made technical advances in fluorescence laser microdissection (LMD) in combination with the absolute quantification of small amounts of mRNAs from a region ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00783
更新日期:2012-01-01 00:00:00
abstract::Multiple approaches utilizing viral and DNA vectors have shown promise in the development of an effective vaccine against HIV. In this study, an alternative replication-defective flavivirus vector, RepliVax (RV), was evaluated for the delivery of HIV-1 immunogens. Recombinant RV-HIV viruses were engineered to stably e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56550-4
更新日期:2019-12-27 00:00:00
abstract::Some 1,3-diarylureas and 1-((1,4-trans)-4-aryloxycyclohexyl)-3-arylureas (cHAUs) activate heme-regulated kinase causing protein synthesis inhibition via phosphorylation of the eukaryotic translation initiation factor 2 (eIF2) in mammalian cancer cells. To evaluate if these agents have potential to inhibit trypanosome ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-23259-9
更新日期:2018-03-20 00:00:00
abstract::The aim was to determine the effects of changing pulse duration and electrode size on muscle contractile properties. Thirty-six healthy young male participated in the study (age 24.8 ± 5.8 years; height 178.2 ± 0.6 cm; body mass 71.8 ± 7.3 kg; self-reported weekly moderate intensity activity 3.5 ± 1.2 h·week-1). Tensi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65111-z
更新日期:2020-05-18 00:00:00
abstract::Cartilage engineering that combines competent seeding cells and a compatible scaffold is increasingly gaining popularity and is potentially useful for the treatment of various bone and cartilage diseases. Intensive efforts have been made by researchers to improve the viability and functionality of seeding cells of eng...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26617
更新日期:2016-05-25 00:00:00