Distinct functional responses to stressors of bone marrow derived dendritic cells from diverse inbred chicken lines.

Abstract:

:Differences in responses of chicken bone marrow derived dendritic cells (BMDC) to in vitro treatment with lipopolysaccharide (LPS), heat, and LPS + heat were identified. The Fayoumi is more disease resistant and heat tolerant than the Leghorn line. Nitric Oxide (NO) production, phagocytic ability, MHC II surface expression and mRNA expression were measured. NO was induced in BMDC from both lines in response to LPS and LPS + heat stimulation; Fayoumi produced more NO with LPS treatment. Fayoumi had higher phagocytic ability and MHC II surface expression. Gene expression for the heat-related genes BAG3, HSP25, HSPA2, and HSPH1 was strongly induced with heat and few differences existed between lines. Expression for the immune-related genes CCL4, CCL5, CD40, GM-CSF, IFN-γ, IL-10, IL-12β, IL-1β, IL-6, IL-8, and iNOS was highly induced in response to LPS and different between lines. This research contributes to the sparse knowledge of genetic differences in chicken BMDC biology and function.

journal_name

Dev Comp Immunol

authors

Van Goor A,Slawinska A,Schmidt CJ,Lamont SJ

doi

10.1016/j.dci.2016.05.016

subject

Has Abstract

pub_date

2016-10-01 00:00:00

pages

96-110

eissn

0145-305X

issn

1879-0089

pii

S0145-305X(16)30170-7

journal_volume

63

pub_type

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