当前位置： SCI文献检索 > DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY期刊下所有文献 > Thioredoxin peroxidase gene is involved in resistance to biocontrol fungus Nomuraea rileyi in Spodoptera litura: gene cloning, expression, localization and function.

Thioredoxin peroxidase gene is involved in resistance to biocontrol fungus Nomuraea rileyi in Spodoptera litura: gene cloning, expression, localization and function.

Abstract：

:Thioredoxin peroxidases (Tpxs) are a ubiquitous family of antioxidant enzymes that play important roles in protecting organisms against oxidative stress. Here, one Tpx was cloned from Spodoptera litura named as SlTpx. The full-length cDNA consists of 1165 bp with 588 bp open reading frame, encoding 195 amino acids. The putative amino acid sequence shared >70% identity with Tpxs from other insects. Phylogenetic analysis revealed that SlTpx is closely related to other available lepidopteran Tpxs. Real-time PCR analysis showed that SlTpx can be induced by Nomuraea rileyi infection in some detected tissues at the mRNA level. The strongest expression was found in hemocytes of unchallenged and N. rileyi-challenged S. litura. Western blotting showed SlTpx protein in the hemocytes, head and cuticle from normal S. litura. However, when N. rileyi was inoculated into the body cavity of S. litura larvae, SlTpx protein was detected in head, hemocytes, fatbody, midgut, malpighian tubule, but not in the hemolymph and cuticle. Moreover, time-course analysis showed that SlTpx mRNA/protein expression levels were up-regulated in the hemocytes, when S. litura were infected by N. rileyi or injected with H2O2. The levels of N. rileyi-induced reactive oxygen species (ROS) in hemocytes were evaluated, and revealed that N. rileyi infection caused generation of ROS, and induced changes in expression of SlTpx. In addition, the heterologously expressed protein of this gene in Escherichia coli showed antioxidant activity; it removed H2O2 and protected DNA. Knocking down SlTpx transcripts by dsRNA interference resulted in accelerated insect death with N. rileyi infection. This is believed to be the first report showing that SlTpx has a significant role in resisting oxidative stress caused by N. rileyi infection.

journal_name

Dev Comp Immunol

journal_title

Developmental and comparative immunology

authors

Chen H,Yin Y,Feng E,Li Y,Xie X,Wang Z

doi

10.1016/j.dci.2013.11.012

subject

Has Abstract

pub_date

2014-05-01 00:00:00

pages

76-85

issue

1

eissn

0145-305X

issn

1879-0089

pii

S0145-305X(13)00333-9

journal_volume

44

pub_type

杂志文章

相关文献

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY文献大全
  • Snail defence responses to parasite infection: The Lymnaea stagnalis-Trichobilharzia szidati model.

    abstract：:Lymnaea stagnalis is a common freshwater gastropod. Importantly, the snail serves as the intermediate host for more than one hundred species of digenetic trematodes, including the avian schistosome Trichobilharzia szidati, a causative agent of cercarial dermatitis in humans. Infection of L. stagnalis by T. szidati ini...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.dci.2019.103464

    authors： Skála V,Walker AJ,Horák P

    更新日期：2020-01-01 00:00:00

  • Changes in mucosal and serum immunoglobulin levels of California sea lions during early development.

    abstract：:To date, most studies on pinniped immunoglobulins have focused on circulating antibodies. However, systemic and local immune activities differ in terms of maturation, intensity, and types of effectors that participate. Here, we examined levels of three immunoglobulin isotypes, IgG, IgM and IgA, in the blood and mucosa...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2017.08.006

    authors： Espinosa-de Aquino W,Bailey M,Acevedo-Whitehouse K

    更新日期：2017-12-01 00:00:00

  • A longitudinal study of the protein components of marsupial milk from birth to weaning in the tammar wallaby (Macropus eugenii).

    abstract：:The major milk whey proteins of the tammar wallaby (Macropus eugenii) have been identified over the total period of lactation using proteomic analysis techniques comprising two-dimensional electrophoresis, comparative image analysis, matrix assisted laser desorption ionisation mass spectrometry (MALDI MS), de novo pep...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2008.08.002

    authors： Joss JL,Molloy MP,Hinds L,Deane E

    更新日期：2009-02-01 00:00:00

  • Tolerance maintenance depends on persistence of the tolerizing antigen: evidence from transplantation studies on Xenopus laevis.

    abstract：:In order to assess the role of antigen persistence in the tolerant state, tolerance was induced in Xenopus laevis by the embryonic transplantation of whole eyes or tail tissue. Both types of transplants were seen to heal in and persist, with no signs of immunological incompatibility. At metamorphosis, tail resorption ...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/0145-305x(83)90034-4

    authors： Kaye C,Schermer JA,Tompkins R

    更新日期：1983-07-01 00:00:00

  • Cxcl8-l1 and Cxcl8-l2 are required in the zebrafish defense against Salmonella Typhimurium.

    abstract：:In recent years zebrafish has emerged as an excellent model for studying the Cxcl8 signaling pathway in inflammation elicited upon tissue damage or infection. Zebrafish has two true homologs of mammalian CXCL8, named Cxcl8-l1 and Cxcl8-l2. Previously, we have shown that in wound-associated inflammation, these chemokin...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2014.11.004

    authors： de Oliveira S,Lopez-Muñoz A,Martínez-Navarro FJ,Galindo-Villegas J,Mulero V,Calado Â

    更新日期：2015-03-01 00:00:00

  • Extracellular vesicles from cod (Gadus morhua L.) mucus contain innate immune factors and deiminated protein cargo.

    abstract：:Extracellular vesicles are released from cells and participate in cell communication via transfer of protein and genetic cargo derived from the parent cells. EVs play roles in normal physiology and immunity and are also linked to various pathological processes. Peptidylarginine deiminases (PADs) are phylogenetically c...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2019.103397

    authors： Magnadóttir B,Kraev I,Guðmundsdóttir S,Dodds AW,Lange S

    更新日期：2019-10-01 00:00:00

  • The effect of aging on T cell responses in the horse.

    abstract：:Horses greater than 20 years of age exhibit alterations in their immune responses similar to those observed in other aged individuals. The purpose of this study was to characterize immunosenescence in a population of aged ponies. The peripheral blood mononuclear cells (PBMC) from aged ponies exhibited a decreased prol...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/s0145-305x(01)00027-1

    authors： Horohov DW,Kydd JH,Hannant D

    更新日期：2002-01-01 00:00:00

  • Trypanosoma cruzi is lysed by coelomic cytolytic factor-1, an invertebrate analogue of tumor necrosis factor, and induces phenoloxidase activity in the coelomic fluid of Eisenia foetida foetida.

    abstract：:A cytolytic protein named Coelomic Cytolytic Factor-1 (CCF-1) was isolated from the coelomic fluid of the earthworm Eisenia foetida foetida. Despite the absence of any gene homology, CCF-1 showed functional analogy with the mammalian cytokine tumour necrosis factor (TNF), particularly based on similar lectin-like acti...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/s0145-305x(01)00048-9

    authors： Olivares Fontt E,Beschin A,Van Dijck E,Vercruysse V,Bilej M,Lucas R,De Baetselier P,Vray B

    更新日期：2002-01-01 00:00:00

  • Hydrocortisone suppresses the chemiluminescent response of striped bass phagocytes.

    abstract：:Phagocytic cells obtained from the pronephros of striped bass (Morone saxatilis) were exposed in vitro to various levels of hydrocortisone acetate. This treatment reduced the normal ability of the cells to generate a chemiluminescent response when exposed to bacteria or phorbol myristate acetate. Although the levels o...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/0145-305x(85)90061-8

    authors： Stave JW,Roberson BS

    更新日期：1985-01-01 00:00:00

  • Lymphocyte markers in the bovine foetus.

    abstract：:The ontogeny of lymphocyte surface markers was studied in thymus, retropharyngeal lymph nodes, spleen, bone marrow, and liver of 48 bovine foetuses, 80-280 days of gestation. The markers examined were the spontaneous (E) rosette against sheep erythrocytes (SRBCs) in foetal calf serum (E/FCS), in medium supplemented wi...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/0145-305x(83)90018-6

    authors： Higgins DA,Stack MJ,Richardson C

    更新日期：1983-04-01 00:00:00

  • The immune system of sea bass, Dicentrarchus labrax, reared in aquaculture.

    abstract：:The sea bass Dicentrarchus labrax is one the most important seawater fish species of south Europe and Mediterranean aquaculture, and studies on its immune system are important for both scientific and applied purposes. In this paper, we summarise the results obtained in studies of the immune system in this species, and...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章,评审

    doi：10.1016/s0145-305x(01)00057-x

    authors： Scapigliati G,Romano N,Buonocore F,Picchietti S,Baldassini MR,Prugnoli D,Galice A,Meloni S,Secombes CJ,Mazzini M,Abelli L

    更新日期：2002-03-01 00:00:00

  • Regional and global changes in TCRalphabeta T cell repertoires in the gut are dependent upon the complexity of the enteric microflora.

    abstract：:The repertoire of gut associated T cells is shaped by exposure to microbes, including the natural enteric microflora. Previous studies compared the repertoire of gut associated T cell populations in germ free (GF) and conventional mammals often focussing on intra-epithelial lymphocyte compartments. Using GF, conventio...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2009.11.009

    authors： Mwangi WN,Beal RK,Powers C,Wu X,Humphrey T,Watson M,Bailey M,Friedman A,Smith AL

    更新日期：2010-04-01 00:00:00

  • The alternatively spliced porcine FcγRI regulated PRRSV-ADE infection and proinflammatory cytokine production.

    abstract：:Receptors for the Fc region of IgG (FcγRs) play a key role in protecting the immune system and host from infection. In this study, we described the cloning, sequencing and characterization of porcine FcγRI, and reported six different FcγRI isoforms, four of which have never been reported before. Further analysis revea...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2018.09.019

    authors： Shi P,Su Y,Li Y,Zhang L,Lu D,Li R,Zhang L,Huang J

    更新日期：2019-01-01 00:00:00

  • The upregulation of immune responses in tyrosine hydroxylase (TH) silenced Litopenaeus vannamei.

    abstract：:Catecholamines (CAs) play a crucial role in maintaining physiological and immune homeostasis in invertebrates and vertebrates under stressful conditions. Tyrosine hydroxylase (TH) is the first and rate-limiting enzyme in CA synthesis. To develop an effective CA-related immunological defense system against stress and p...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2016.11.002

    authors： Mapanao R,Chang CC,Cheng W

    更新日期：2017-02-01 00:00:00

  • Distinct functional responses to stressors of bone marrow derived dendritic cells from diverse inbred chicken lines.

    abstract：:Differences in responses of chicken bone marrow derived dendritic cells (BMDC) to in vitro treatment with lipopolysaccharide (LPS), heat, and LPS + heat were identified. The Fayoumi is more disease resistant and heat tolerant than the Leghorn line. Nitric Oxide (NO) production, phagocytic ability, MHC II surface expre...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2016.05.016

    authors： Van Goor A,Slawinska A,Schmidt CJ,Lamont SJ

    更新日期：2016-10-01 00:00:00

  • Molecular characterization and expression analysis of four isotypes of immunoglobulin light chain genes in orange-spotted grouper, Epinephelus coioides.

    abstract：:To date, many immunoglobulin (Ig) genes have been identified in diverse teleost species, but the contributions of different types of light chain (IgL) to the immune response remain unclear. Screening of a stimulated kidney cDNA library from orange-spotted grouper (Osg, Epinephelus coioides) resulted in the identificat...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2012.11.003

    authors： Wu MS,Cheng CA,Lin CH,Lee CY,Tseng SJ,Tzeng CS,Chang CY

    更新日期：2013-03-01 00:00:00

  • Expression of perforin-granzyme pathway genes in the bursa of infectious bursal disease virus-infected chickens.

    abstract：:Infectious bursal disease (IBD) is an economically important immunosuppressive disease of chickens. The IBD virus (IBDV) actively replicates in B cells and causes severe bursal damage. Generally, T cells are refractory to infection with IBDV but are known to promote virus clearance. However, the mechanisms of T cell m...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2011.01.007

    authors： Rauf A,Khatri M,Murgia MV,Saif YM

    更新日期：2011-05-01 00:00:00

  • Molecular characterization and expression analysis of TLR1 and TLR4 from the endangered fish Dabry's sturgeon (Acipenser dabryanus).

    abstract：:Toll-like receptors (TLRs) are important pattern recognition receptors (PRRs) of teleost innate immune system. However, information about TLRs is absent in Dabry's sturgeon (Acipenser dabryanus), one of the most primitive actinopterygii species. In the present study, the full lengths of adaTLR1 and adaTLR4 were cloned...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2018.05.009

    authors： Han P,Wang S,Zhang Q,Zhang S,Shao R,Xu W,Zhang W,Xu Q,Wei Q,Qi Z

    更新日期：2018-09-01 00:00:00

  • The novel six LIM and one PET domain-containing protein Lmpt is involved in the immune response through activation of the NF-κB signalling pathway in the crustacean, Macrobrachium nipponense.

    abstract：:The four-and-a-half LIM-only protein family of transcription co-factors participates in various cellular processes, such as cell proliferation, cell differentiation, apoptosis, cell adhesion, migration, transcription and signal transduction. However, the knowledge of the structural characteristics and immune functions...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2020.103948

    authors： Xu L,Zhou X,Wang P,Jin Q,Zhu T,Chen M,Xu H

    更新日期：2021-03-01 00:00:00

  • Characterisation of chicken OX40 and OX40L.

    abstract：:The Tumour Necrosis Factor superfamilies of receptors and ligands play a crucial role in the regulation of effective immune responses against pathogens and malignant cells. In chickens, only few members have been identified. Here, we characterise the chicken homologues for mammalian costimulatory molecules OX40 and OX...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2018.01.014

    authors： Scherer S,Göbel TW

    更新日期：2018-05-01 00:00:00

  • Tissue-specific reduction in DC-SIGN expression correlates with progression of pathogenic simian immunodeficiency virus infection.

    abstract：:Studies were undertaken to determine whether previously described reductions in splenic DC-SIGN expression in simian acquired immune deficiency syndrome (AIDS) are limited to pathogenic simian immunodeficiency virus (SIV) infection. DC-SIGN expression was evaluated by immunohistochemistry in lymphoid tissues from AIDS...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2008.06.006

    authors： Yearley JH,Kanagy S,Anderson DC,Dalecki K,Pauley DR,Suwyn C,Donahoe RM,McClure HM,O'Neil SP

    更新日期：2008-01-01 00:00:00

  • Light chain variable region diversity in Atlantic cod (Gadus morhua L.).

    abstract：:This study was undertaken to determine if a lack of V(L) domain variability could explain, in part, the failure of Atlantic cod to respond to immunization with the production of specific antibodies. The variability of cod V(L) regions was studied in 33 cDNA and two genomic clones. The variability of the CDRs was estim...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/s0145-305x(99)00003-8

    authors： Widholm H,Lundbäck AS,Daggfeldt A,Magnadottir B,Warr GW,Pilström L

    更新日期：1999-04-01 00:00:00

  • 2E3, a new marker that selectively identifies porcine CD4+ naive T cells.

    abstract：:We describe a novel antigen recognized by mAb 2E3 selectively expressed in the periphery by a subset of porcine CD4+ T cells. Both, CD4+CD8alpha- and CD4+CD8alphalow T cell subpopulations express this antigen. CD4+2E3+ T cells show phenotypical and functional characteristics of nai;ve cells. The majority of them are C...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/s0145-305x(03)00137-x

    authors： Revilla C,Rodríguez-Carreño MP,Alvarez B,Chamorro S,Alonso LM,Ezquerra A,Alonso F,Domínguez J

    更新日期：2004-03-01 00:00:00

  • Partial characterization of chicken spleen cell culture supernatants stimulated with Staphylococcus aureus.

    abstract：:The role of accessory cells in the proliferative response of chicken spleen cells to Staphylococcus aureus Cowan I (SAC) was examined. It was found that chicken spleen cells cultured with SAC produced a soluble molecule capable of causing proliferation when culture supernatants were added to spleen cells. The molecule...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/0145-305x(87)90020-6

    authors： Schoof DD,Tempelis CH

    更新日期：1987-01-01 00:00:00

  • The ontogeny of the porcine immune system.

    abstract：:Cellular and humoral aspects of the immune response develop sequentially in the fetus. During the ontogeny, the pluripotent stem cells emerge and differentiate into all hematopoietic lineages. Basic questions including the identification of the first lympho-hematopoietic sites, the origin of T and B lymphocytes, the d...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.dci.2008.07.011

    authors： Sinkora M,Butler JE

    更新日期：2009-03-01 00:00:00

  • Transduction of binding affinity by B lymphocytes: a new dimension in immunological regulation.

    abstract：:To date, immunologists have operated with two primary paradigms governing the antibody response: (1) that affinity maturation is primarily dependent upon antigen-driven selection of both the germline and somatically amended repertoires, and (2) that antibody effector function is isotypically determined. The teleost mo...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.dci.2011.01.015

    authors： Ye J,Bromage E,Kaattari I,Kaattari S

    更新日期：2011-09-01 00:00:00

  • Serpin-15 from Bombyx mori inhibits prophenoloxidase activation and expression of antimicrobial peptides.

    abstract：:Serine protease inhibitors (SPIs) play a key role in physiological responses by controlling protease activities. In this study, we studied the biochemical functions of serpin-15, an SPI, from Bombyx mori (Bmserpin-15). Recombinant Bmserpin-15 was expressed in Escherichia coli cells and used to raise rabbit anti-Bmserp...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2015.02.013

    authors： Liu D,Wang L,Yang L,Qian C,Wei G,Dai L,Li J,Zhu B,Liu C

    更新日期：2015-07-01 00:00:00

  • Immunopathogenesis of haemorrhagic enteritis virus (HEV) in turkeys.

    abstract：:Infection of turkeys with the haemorrhagic enteritis virus (HEV), a type II avian adenovirus, results in varying rates of morbidity and mortality. The disease is characterised by splenomegaly, intestinal haemorrhage, sudden death and immunosuppression. The mechanisms of HEV immunopathogenesis and immunosuppression are...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章,评审

    doi：10.1016/s0145-305x(99)00075-0

    authors： Rautenschlein S,Sharma JM

    更新日期：2000-03-01 00:00:00

  • Dynamics of immune cell infiltration in the caecal lamina propria of chickens after neonatal infection with a Salmonella enteritidis strain.

    abstract：:Dynamics of leucocyte infiltration and bacterial invasion in the caecal wall were studied after oral infection of 2-day-old chicks with Salmonella enterica ser. Enteritidis. Bacteria invaded the lamina propria of the caecal wall from 12h post-challenge onwards. Bacteriological examination of internal organs (liver, sp...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/s0145-305x(01)00084-2

    authors： Van Immerseel F,De Buck J,De Smet I,Mast J,Haesebrouck F,Ducatelle R

    更新日期：2002-05-01 00:00:00

  • Developmental expression of alloantigen systems in the chicken.

    abstract：:The different allelic forms of nine non-Mhc alloantigen systems of the chicken were examined for developmental expression on erythrocytes isolated from embryos and young chicks. Polyclonal alloantisera raised against the different antigens were used to detect these antigens on the cell surface by hemagglutination as w...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/s0145-305x(99)00042-7

    authors： Kopti F,Stevens SL,Briles WE

    更新日期：1999-10-01 00:00:00