Abstract:
:This study was undertaken to determine if a lack of V(L) domain variability could explain, in part, the failure of Atlantic cod to respond to immunization with the production of specific antibodies. The variability of cod V(L) regions was studied in 33 cDNA and two genomic clones. The variability of the CDRs was estimated by the Shannon entropy method and compared with that in other species. It was found to be lowest in the little skate (Raja erinacea), higher in cod, and highest in Xenopus and mouse. While the variability of the CDRs is slightly lower in cod than in Xenopus and mouse, it is spread over broader areas of the amino acid sequence. The length of CDR1 and CDR3 in cod is equal to or exceeds that found in skate, Xenopus, chicken and mammals. Isoelectric points and hydrophobicity vary substantially among the studied Ig light chain domains. Thus, neither the length, nor the variability, nor the physicochemical properties (pI and hydrophobicity) of the L chain CDRs can explain the absence of antibody response to immunization in cod.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Widholm H,Lundbäck AS,Daggfeldt A,Magnadottir B,Warr GW,Pilström Ldoi
10.1016/s0145-305x(99)00003-8keywords:
subject
Has Abstractpub_date
1999-04-01 00:00:00pages
231-40issue
3eissn
0145-305Xissn
1879-0089pii
S0145-305X(99)00003-8journal_volume
23pub_type
杂志文章abstract::Fish nonspecific cytotoxic cells (NCC) lyse various transformed human B-cells (NC-37, P3HR-1) and erythroblastoid cells (K562) as well as mouse YAC-1 and P815 cells. Highest NCC activity was found in the anterior (head) kidney, but spleen cells and peripheral blood leucocytes (PBL) also demonstrated cytolytic abilitie...
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journal_title:Developmental and comparative immunology
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journal_title:Developmental and comparative immunology
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journal_title:Developmental and comparative immunology
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journal_title:Developmental and comparative immunology
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