Abstract：
:Cytidine/uridine monophosphate kinase 2 (CMPK2) is a thymidylate kinase and in mammals is known to be involved in mitochondrial DNA (mtDNA) synthesis and antiviral immunity. However, very little is known about the function of CMPK2 in fish. With an aim to elucidate the antimicrobial mechanism of CMPK2 in fish, we in this study examined the function of CMPK2 from triploid crucian carp (3nCmpk2). 3nCmpk2 is 426 residues in length and possesses the conserved thymidylate kinase domain. The deduced amino acid sequence of 3nCmpk2 shares 53.2%-99.1% overall identities with the CMPK2 of several fish species. Quantitative real time RT-PCR (qRT-PCR) analysis showed that 3nCmpk2 expression occurred in multiple tissues and was upregulated by bacterial infection in a time-dependent manner. Recombinant 3nCmpk2 (r3nCmpk2) induced mtDNA synthesis and NLRP3 activation. Overexpression of 3nCmpk2 protects the intestinal barrier and hampers the bacterial colonization in fish tissues. These results provide the first evidence that 3nCmpk2 is involved in host innate immunity and plays a protective role in antimicrobial responses during bacterial infection.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Feng C,Tang Y,Liu X,Zhou Zdoi
10.1016/j.dci.2020.103924subject
Has Abstractpub_date
2021-03-01 00:00:00pages
103924eissn
0145-305Xissn
1879-0089pii
S0145-305X(20)30479-1journal_volume
116pub_type
杂志文章abstract：:CD8, belonging to the TCR complex, is the main marker molecule of CTLs. Although CD8 genes have been detected in many fish species, the analysis of teleost CD8+ cells has been limited because of the lack of antibodies. Using newly established mAbs against rainbow trout CD8α, we found high ratios of CD8α+ cells in trou...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2011.02.008
更新日期：2011-07-01 00:00:00
abstract：:Phenoloxidase (PO) plays an important role in arthropod melanization. Previously, a prophenoloxidase (PmproPO1) gene was cloned and characterized from the hemocytes of the black tiger shrimp, Penaeus monodon. In the present study, we report a novel proPO gene (PmproPO2) belonging to the proPO family identified from th...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2008.09.003
更新日期：2009-02-01 00:00:00
abstract：:This study was undertaken to determine if a lack of V(L) domain variability could explain, in part, the failure of Atlantic cod to respond to immunization with the production of specific antibodies. The variability of cod V(L) regions was studied in 33 cDNA and two genomic clones. The variability of the CDRs was estim...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/s0145-305x(99)00003-8
更新日期：1999-04-01 00:00:00
abstract：:The purpose of this study was to investigate in rats, by double-label immunofluorescence and flow cytometric analysis, the age related changes in the CD4 subset of gut-associated lymphoid tissues and spleen. We found that the percentage of CD4+ T cells in Peyer's patches (PP) and spleen (SP) increased during the first...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/s0145-305x(97)00023-2
更新日期：1997-09-01 00:00:00
abstract：:Temperature variability, and in particular temperature decreases, can increase susceptibility of amphibians to infections by the fungus Batrachochytrium dendrobatidis (Bd). However, the effects of temperature shifts on the immune systems of Bd-infected amphibians are unresolved. We acclimated frogs to 16 °C and 26 °C ...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2017.08.018
更新日期：2017-12-01 00:00:00
abstract：:Immune checkpoint immunotherapy is a pillar of human oncology treatment with potential for non-human species. The first checkpoint immunotherapy approved for human cancers targeted the CTLA4 protein. CTLA4 can inhibit T cell activation by capturing and internalizing CD80 and CD86 from antigen presenting cells, a proce...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2020.103882
更新日期：2021-02-01 00:00:00
abstract：:Hemocyanin is a respiratory protein that possesses multiple physiological and immunological functions in shrimp. However, the transcriptional regulation of the hemocyanin gene is still poorly understood. Here, the nuclear receptor E75 of Litopenaeus vannamei (LvE75) was identified as one of the transcriptional regulat...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2020.103662
更新日期：2020-06-01 00:00:00
abstract：:RIG-I like receptor (RLR) signaling functions importantly in host innate immune response against RNA virus, which is tightly regulated by a number of mechanisms to prevent aberrant interferon production. The suppressor of IKKε (SIKE) has been identified as a suppressor of IKKε and TBK1, which are key components of RLR...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2018.09.016
更新日期：2019-01-01 00:00:00
abstract：:The lectin complement pathway has important functions in vertebrate host defence and accumulating evidence of primordial complement components trace its emergence to invertebrate phyla. We introduce two putative mannose-binding lectin homologues (CioMBLs) from the urochordate species Ciona intestinalis. The CioMBLs di...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2009.08.004
更新日期：2010-01-01 00:00:00
abstract：:Single WAP domain (SWD)-containing proteins are small proteins with a C-terminal region containing a single whey acidic protein (WAP) domain. In the present study, the cDNAs representing three isoforms of SWD proteins (SWDPm1, SWDPm2 and SWDPm3) were identified from hemocytes of the black tiger shrimp, Penaeus monodon...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2008.06.005
更新日期：2008-01-01 00:00:00
abstract：:In mammals, the toll-like receptors (TLRs) play a major role in initiating innate immune responses against pathogens. Comparison of the TLRs in different mammals may help in understanding the TLR-mediated responses and developing of animal models and efficient therapeutic measures for infectious diseases. The Chinese ...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2016.02.025
更新日期：2016-07-01 00:00:00
abstract：:We have studied the chicken's pineal gland by light and electron microscopy at 3 and 4 weeks of age. The results indicated that lymphocytes, plasma cells, secretory cells, basophil and eosinophil granulocytes enter the parenchyma and a new histologically defined tissue is formed which we call lymphopineal tissue. Insi...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(84)90068-5
更新日期：1984-10-01 00:00:00
abstract：:In vertebrates, a total of eleven interferon (IFN) regulatory factors (IRFs), IRF1 to IRF11 are reported, with the conserved presence of IRF1 to IRF9 in all classes of vertebrates. However, IRF10 has been reported only in fish and birds, and IRF11 seems to be a fish specific IRF member. In this study, IRF11 in mandari...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2020.103846
更新日期：2021-01-01 00:00:00
abstract：:Flow cytometry is a common approach to study invertebrate immune cells including earthworm coelomocytes. However, the link between light-scatter- and microscopy-based phenotyping remains obscured. Here we show, by means of light scatter-based cell sorting, both subpopulations (amoebocytes and eleocytes) can be physica...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2016.06.017
更新日期：2016-12-01 00:00:00
abstract：:A new lily-type lectin RbLTL was identified from rock bream (Oplegnathus fasciatus) and its expression analysed. In this study, a new lily-type lectin gene (RbLTL) was cloned from rock bream using expressed sequence tag (EST) analysis. The full-length RbLTL cDNA was encoding a 117-amino acid protein. The deduced amino...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2016.06.014
更新日期：2016-12-01 00:00:00
abstract：:The development of the liver, bone marrow and spleen have been investigated in the stripe-faced dunnart. At birth, the liver was undergoing haematopoiesis but the level declined rapidly and by day 50 after birth the liver was histologically mature. Both the bone marrow and spleen were non-haematopoietic at birth but i...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2003.08.004
更新日期：2004-04-01 00:00:00
abstract：:Nonspecific cytotoxic cells (NCC) obtained from the head (anterior) kidney of fish (Ictalurus punctatus) lyse human transformed B-cell targets. Lysis depended on direct cell-cell contact. Fish size, age, environmental holding temperatures, and lytic reaction conditions such as osmolality and optimum effector:target ce...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(84)90036-3
更新日期：1984-04-01 00:00:00
abstract：:Small heat shock proteins are a molecular chaperone and implicated in various physiological and stress processes in animals. However, the immunological functions of Hsp genes remain to elucidate in the crustaceans, particularly in red swamp crayfish, Procambarus clarkii. Here we report the cloning of heat shock protei...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2020.103755
更新日期：2020-10-01 00:00:00
abstract：:Melanization mediated by the prophenoloxidase-activating system (proPO) is an important immune response in invertebrates. However, in addition to melanin, the proPO system produces reactive intermediates that are not only harmful to the invading microbes but also to the host cells. Thus, the proPO system must be tight...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2017.02.014
更新日期：2017-07-01 00:00:00
abstract：:β-glucans exert receptor-mediated immunomodulating activities, including oxidative burst activity and cytokine secretion. The role of the β-glucan receptors dectin-1 and complement receptor 3 (CR3) in the response of immune cells towards β-glucans is still unresolved. Dectin-1 is considered as the main β-glucan recept...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2014.10.005
更新日期：2015-01-01 00:00:00
abstract：:The aim of this work was to identify in Eisenia fetida genes whose expression are regulated following exposure to a complex mixture of metallic trace elements (MTE) representative of a highly polluted smelter soil. Suppression subtractive hybridization (SSH) was used to construct cDNA libraries enriched in up- or down...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2008.06.009
更新日期：2008-01-01 00:00:00
abstract：:In order to assess the role of antigen persistence in the tolerant state, tolerance was induced in Xenopus laevis by the embryonic transplantation of whole eyes or tail tissue. Both types of transplants were seen to heal in and persist, with no signs of immunological incompatibility. At metamorphosis, tail resorption ...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(83)90034-4
更新日期：1983-07-01 00:00:00
abstract：:Phagocytosis provides a critical first line of defense against invading pathogens. Engagement of particles through receptor-mediated binding precedes internalization and induction of cellular antimicrobial responses. Phagocytes have the capacity to differentially regulate binding and internalization processes through ...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2010.06.006
更新日期：2010-11-01 00:00:00
abstract：:This study was performed to identify the expression patterns of the cathelicidin genes in a local chicken breed and to evaluate the antimicrobial activities of the cathelicidin peptides against pathogenic bacteria. This analysis revealed that the coding regions of CATH-1, -2, and -3 genes contain 447 bp, 465 bp, and 4...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2016.06.012
更新日期：2016-12-01 00:00:00
abstract：:The study of the genetic regulation of infectious disease resistance depends on the availability of inbred lines or selection lines of the species under investigation. The small numbers of such lines of fish has limited the strategy in teleosts to studies of associations between disease and immune/health traits. Attem...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章,评审
doi：10.1016/s0145-305x(96)00032-8
更新日期：1996-11-01 00:00:00
abstract：:Stimulator of interferon (IFN) genes (STING), also known as MPYS/MITA/ERIS/TMEM173, is a recently discovered adaptor protein that functions downstream of RIG-I and upstream of TBK1 and plays an important role in type I interferon (IFN) production. Mammalian STINGs have been isolated from human, mouse, pig, cattle and ...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2012.02.010
更新日期：2012-07-01 00:00:00
abstract：:A range of marine invertebrates were screened for prophenoloxidase (a marker protein of the prophenoloxidase activating system) in the coelomic fluid or hemolymph. The crustaceans and the ascidian, Ciona intestinalis, displayed strongest activity, with low levels found in the starfish, Asterias rubens, the sea urchin,...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(91)90018-t
更新日期：1991-10-01 00:00:00
abstract：:Few pathogens have shaped human medicine as the mycobacteria. From understanding biological phenomena driving disease spread, to mechanisms of host-pathogen interactions and antibiotic resistance, the Mycobacterium genus continues to challenge and offer insights into the basis of health and disease. Teleost fish model...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章,评审
doi：10.1016/j.dci.2019.02.011
更新日期：2019-07-01 00:00:00
abstract：:A five-domain Kazal-type serine proteinase inhibitor, SPIPm2, from Penaeus monodon has recently been implicated in antiviral responses for it is up-regulated upon viral infection and needs further studies. The SPIPm2 genomic gene was composed of seven exons and six introns. The genomic DNA segments coding for each Kaz...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2010.06.001
更新日期：2010-10-01 00:00:00
abstract：:Recently, it has been shown that the salmon louse (Lepeophtheirus salmonis) is commonly infected by one or two vertically transmitted Lepeophtheirus salmonis rhabdoviruses (LsRVs). As shown in the present study, the viruses have limited effect on louse survival, developmental rate and fecundity. Since the LsRVs were c...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2018.04.023
更新日期：2018-09-01 00:00:00
