Abstract:
:The aim of this work was to identify in Eisenia fetida genes whose expression are regulated following exposure to a complex mixture of metallic trace elements (MTE) representative of a highly polluted smelter soil. Suppression subtractive hybridization (SSH) was used to construct cDNA libraries enriched in up- or down-regulated transcripts in the immune-circulating cells of the coelomic cavities, namely coelomocytes, from worms exposed to metallic pollution. Among 1536 SSH-derived cDNA clones sequenced, we identified 764 unique ESTs of which we selected 18 candidates on the basis of their redundancy. These selected candidates were subjected to a two-step validation procedure based on the study of their expression level by real-time PCR. The first step consisted in measuring the expression of the 18 candidates in worms exposed to artificial contaminated soil. The second step consisted in measuring the expression in animals exposed to a "naturally" contaminated soil sampled close to a smelter. Both steps allowed us to highlight 3 candidates that are strongly induced in worms exposed to a smelter polluted soil. These candidates are: the well-known MTE-induced Cd-metallothionein and 2 original biomarkers, lysenin, and a transcript, which cloning of the complete coding sequence identified as the coactosin-like protein.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Brulle F,Cocquerelle C,Mitta G,Castric V,Douay F,Leprêtre A,Vandenbulcke Fdoi
10.1016/j.dci.2008.06.009subject
Has Abstractpub_date
2008-01-01 00:00:00pages
1441-53issue
12eissn
0145-305Xissn
1879-0089pii
S0145-305X(08)00149-3journal_volume
32pub_type
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journal_title:Developmental and comparative immunology
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journal_title:Developmental and comparative immunology
pub_type: 杂志文章,评审
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journal_title:Developmental and comparative immunology
pub_type: 杂志文章,评审
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journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2008.04.007
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abstract::Type I interferon (IFN) induces the antiviral response in innate immunity. The type I IFN gene cloned from Japanese flounder (Paralichthys olivaceus) has a length of 1189 bp and consisting of 5 exons and 4 introns. In a phylogenetic tree of type I IFNs, Japanese flounder grouped with other Acanthopterygii. To gain ins...
journal_title:Developmental and comparative immunology
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journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2012.05.003
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journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2017.05.016
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journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(84)90102-2
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journal_title:Developmental and comparative immunology
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journal_title:Developmental and comparative immunology
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journal_title:Developmental and comparative immunology
pub_type: 杂志文章
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更新日期:1998-07-01 00:00:00
abstract::Dengue virus has become one of the most important arboviral pathogens affecting the world today. The virus is transmitted among humans by the mosquitoes Aedes aegypti and Ae. albopictus. Like other vector-borne pathogens, this virus encounters innate immune defenses within the mosquito vector that limit infection. We ...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2010.01.006
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abstract::A cytolytic protein named Coelomic Cytolytic Factor-1 (CCF-1) was isolated from the coelomic fluid of the earthworm Eisenia foetida foetida. Despite the absence of any gene homology, CCF-1 showed functional analogy with the mammalian cytokine tumour necrosis factor (TNF), particularly based on similar lectin-like acti...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
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journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2010.06.005
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journal_title:Developmental and comparative immunology
pub_type: 杂志文章
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journal_title:Developmental and comparative immunology
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journal_title:Developmental and comparative immunology
pub_type: 杂志文章
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journal_title:Developmental and comparative immunology
pub_type: 杂志文章
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更新日期:2014-03-01 00:00:00
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journal_title:Developmental and comparative immunology
pub_type: 杂志文章
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更新日期:1996-07-01 00:00:00
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journal_title:Developmental and comparative immunology
pub_type: 杂志文章
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