Identification and expression profile of gene transcripts differentially expressed during metallic exposure in Eisenia fetida coelomocytes.

Abstract:

:The aim of this work was to identify in Eisenia fetida genes whose expression are regulated following exposure to a complex mixture of metallic trace elements (MTE) representative of a highly polluted smelter soil. Suppression subtractive hybridization (SSH) was used to construct cDNA libraries enriched in up- or down-regulated transcripts in the immune-circulating cells of the coelomic cavities, namely coelomocytes, from worms exposed to metallic pollution. Among 1536 SSH-derived cDNA clones sequenced, we identified 764 unique ESTs of which we selected 18 candidates on the basis of their redundancy. These selected candidates were subjected to a two-step validation procedure based on the study of their expression level by real-time PCR. The first step consisted in measuring the expression of the 18 candidates in worms exposed to artificial contaminated soil. The second step consisted in measuring the expression in animals exposed to a "naturally" contaminated soil sampled close to a smelter. Both steps allowed us to highlight 3 candidates that are strongly induced in worms exposed to a smelter polluted soil. These candidates are: the well-known MTE-induced Cd-metallothionein and 2 original biomarkers, lysenin, and a transcript, which cloning of the complete coding sequence identified as the coactosin-like protein.

journal_name

Dev Comp Immunol

authors

Brulle F,Cocquerelle C,Mitta G,Castric V,Douay F,Leprêtre A,Vandenbulcke F

doi

10.1016/j.dci.2008.06.009

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

1441-53

issue

12

eissn

0145-305X

issn

1879-0089

pii

S0145-305X(08)00149-3

journal_volume

32

pub_type

杂志文章
  • Identification and characterization of a novel phage-type like lysozyme from Manila clam, Ruditapes philippinarum.

    abstract::A novel lysozyme gene (RpLysPh) with high similarity to the bacteriophage lysozymes was identified in Manila clam, Ruditapes philippinarum. The full length cDNA of RpLysPh is 828bp and contains a 462bp open reading frame (ORF) that codes for a 154 amino acid protein. Multiple sequence alignment analysis revealed that ...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2014.06.013

    authors: Ding J,Wang R,Yang F,Zhao L,Qin Y,Zhang G,Yan X

    更新日期:2014-11-01 00:00:00

  • Comparison of suppressor cells obtained from tumor-allosensitized adult and untreated neonatal mice.

    abstract::Splenic suppressor cells from neonatal ("intrinsic") and adult (tumor-allosensitized) mice were compared. Both are non-antigen specific and non-H-2 restricted, and are Ly1+2+, I-J+, QA2+ T-cells. Injection of adult peritoneal exudate cells into neonates or adult tumor-allosensitized mice decreases suppressor cell acti...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/0145-305x(85)90066-7

    authors: Argyris BF

    更新日期:1985-01-01 00:00:00

  • Expression and function of triggering receptor expressed on myeloid cells-1 (TREM-1) on canine neutrophils.

    abstract::The dog is both a valued veterinary species and a widely used translational model for sepsis research. However, relatively little work has been performed evaluating potential biomarkers present during canine infection. Triggering receptor expressed on myeloid cells-1 (TREM-1) has shown promise as a biomarker for infec...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2011.03.021

    authors: Li J,Birkenheuer AJ,Marr HS,Levy MG,Yoder JA,Nordone SK

    更新日期:2011-08-01 00:00:00

  • The effect of aging on T cell responses in the horse.

    abstract::Horses greater than 20 years of age exhibit alterations in their immune responses similar to those observed in other aged individuals. The purpose of this study was to characterize immunosenescence in a population of aged ponies. The peripheral blood mononuclear cells (PBMC) from aged ponies exhibited a decreased prol...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/s0145-305x(01)00027-1

    authors: Horohov DW,Kydd JH,Hannant D

    更新日期:2002-01-01 00:00:00

  • C-type lectin B (SpCTL-B) regulates the expression of antimicrobial peptides and promotes phagocytosis in mud crab Scylla paramamosain.

    abstract::As pattern recognition receptors, C-type lectins (CTLs) play important roles in immune system of crustaceans through identifying and binding to the conservative pathogen-associated molecular patterns (PAMPs) on pathogen surfaces. In this study, a new CTL, SpCTL-B, was identified from the hemocytes of mud crab Scylla p...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2018.02.016

    authors: Wei X,Wang L,Sun W,Zhang M,Ma H,Zhang Y,Zhang X,Li S

    更新日期:2018-07-01 00:00:00

  • Function associated molecules on nonspecific cytotoxic cells: role in calcium signaling, redirected lysis, and modulation of cytotoxicity.

    abstract::Monoclonal antibodies (mAbs) specific for a putative antigen receptor (function associated molecule, FAM) on fish NCC were tested for their ability to initiate signal transduction responses. Anti-FAM hybridoma target cells were significantly lysed by NCC when tested by redirected lysis (RL). Redirected lysis was enhan...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/0145-305x(92)90040-j

    authors: Evans DL,Harris DT,Jaso-Friedmann L

    更新日期:1992-09-01 00:00:00

  • MHC class I BFIV gene polymorphisms in four Chinese native chicken breeds.

    abstract::The major histocompatibility complex (MHC) includes the most polymorphic genes in vertebrates, and balancing selection has been proposed as a main evolutionary force. Here we present one of the first data sets examining the genetic characteristics of chicken MHC I BFIV molecules in four Chinese native breeds, sourced ...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2016.05.004

    authors: Dai Y,Liu XL,Tang QF,Hu XM,Shen XH,Zhang DJ

    更新日期:2016-09-01 00:00:00

  • Crustins: enigmatic WAP domain-containing antibacterial proteins from crustaceans.

    abstract::Crustins are antibacterial proteins of ca. 7-14 kDa with a characteristic four-disulphide core-containing whey acidic protein (WAP) domain, expressed by the circulating haemocytes of crustaceans. Over 50 crustin sequences have been now reported from a variety of decapods, including crabs, lobsters, shrimp and crayfish...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章,评审

    doi:10.1016/j.dci.2007.12.002

    authors: Smith VJ,Fernandes JM,Kemp GD,Hauton C

    更新日期:2008-01-01 00:00:00

  • The molecular evolution of the interleukin-1 family of cytokines; IL-18 in teleost fish.

    abstract::The cytokine network is an important homeostatic system with potent activities in immune surveillance, growth, developmental and repair processes. Although interleukin-1beta (IL-1beta) is considered a pivotal pro-inflammatory cytokine, merely focussing on its inflammatory role would be too narrow an approach. Elucidat...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章,评审

    doi:10.1016/j.dci.2003.09.005

    authors: Huising MO,Stet RJ,Savelkoul HF,Verburg-van Kemenade BM

    更新日期:2004-05-03 00:00:00

  • Limiting immunopathology: Interaction between carotenoids and enzymatic antioxidant defences.

    abstract::The release of reactive oxygen and nitrogen species (ROS and RNS) during the inflammatory response generates damages to host tissues, referred to as immunopathology, and is an important factor in ecological immunology. The integrated antioxidant system, comprising endogenous antioxidant enzymes (e.g. superoxide dismut...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2014.12.007

    authors: Babin A,Saciat C,Teixeira M,Troussard JP,Motreuil S,Moreau J,Moret Y

    更新日期:2015-04-01 00:00:00

  • Identification of the up-regulated expression genes in hemocytes of variously colored abalone (Haliotis diversicolor Reeve, 1846) challenged with bacteria.

    abstract::Variously colored abalone (Haliotis diversicolor Reeve, 1846), which is an important commercial aquatic species and has been widely cultured, frequently suffers from bacterial infection. Knowledge of the defense mechanism in this animal is still lacking and, so far few genes related to immune responses in abalones hav...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2008.04.007

    authors: Wang KJ,Ren HL,Xu DD,Cai L,Yang M

    更新日期:2008-01-01 00:00:00

  • Transcriptional regulation of type I interferon gene expression by interferon regulatory factor-3 in Japanese flounder, Paralichthys olivaceus.

    abstract::Type I interferon (IFN) induces the antiviral response in innate immunity. The type I IFN gene cloned from Japanese flounder (Paralichthys olivaceus) has a length of 1189 bp and consisting of 5 exons and 4 introns. In a phylogenetic tree of type I IFNs, Japanese flounder grouped with other Acanthopterygii. To gain ins...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2011.10.008

    authors: Ohtani M,Hikima J,Hwang SD,Morita T,Suzuki Y,Kato G,Kondo H,Hirono I,Jung TS,Aoki T

    更新日期:2012-04-01 00:00:00

  • Molecular identification and expression analysis of TAB1 from orange-spotted grouper (Epinephelus coioides).

    abstract::Transforming growth factor-β activated kinase 1 (TAK1) is a crucial signal transducer in multiple signaling pathways. TAK1 binds TAB1, TAB2, and TAB3, which act as activators and adaptors that specifically regulate the activation of TAK1. To date, the role of TABs is largely unknown in fish. In the present study, a TA...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2018.09.014

    authors: Hu YZ,Li X,Han R,Jiang B,Li YW,Dan XM,Li AX

    更新日期:2019-01-01 00:00:00

  • Comparative genomics analysis of five families of antimicrobial peptide-like genes in seven ant species.

    abstract::Ants, as eusocial insects, live in dense groups with high connectivity, increasing the risk of pathogen spread and possibly driving the evolution of their antimicrobial immune system. Draft genomes of seven ant species provide a new source to undertake comparative study of their antimicrobial peptides (AMPs), key comp...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2012.05.003

    authors: Zhang Z,Zhu S

    更新日期:2012-10-01 00:00:00

  • Vaccination with UV-inactivated nodavirus partly protects European sea bass against infection, while inducing few changes in immunity.

    abstract::Developing viral vaccines through the ultraviolet (UV) inactivation of virus is promising technique since it is straightforward and economically affordable, while the resulting viruses are capable of eliciting an adequate antiviral immune response. Nodavirus (NNV) is a devastating virus that mainly affects European se...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2018.05.013

    authors: Valero Y,Mokrani D,Chaves-Pozo E,Arizcun M,Oumouna M,Meseguer J,Esteban MÁ,Cuesta A

    更新日期:2018-09-01 00:00:00

  • Vaccination with multimeric recombinant VP28 induces high protection against white spot syndrome virus in shrimp.

    abstract::To improve the efficacy of WSSV protection, multimeric (tetrameric) recombinant VP28 (4XrVP28) was produced and tested in comparison with those of monomeric VP28 (1XrVP28). In vitro binding of either 1XrVP28 or 4XrVP28 to shrimp hemocyte surface was evident as early as 10 min after protein inoculation. Similar results...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2017.05.016

    authors: Taengchaiyaphum S,Nakayama H,Srisala J,Khiev R,Aldama-Cano DJ,Thitamadee S,Sritunyalucksana K

    更新日期:2017-11-01 00:00:00

  • Morphometric analysis of thymic medullary non-lymphoid cell changes during postnatal development.

    abstract::Thymic non-lymphoid cells have been shown to influence the differentiation of T-lymphocytes. Large numbers of non-lymphoid cells are concentrated in the thymic medullary zone. A morphometric analysis of these cells in the medulla identified little change in their absolute number and volume density during thymic develo...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/0145-305x(84)90102-2

    authors: Higley HR,O'Morchoe CC

    更新日期:1984-07-01 00:00:00

  • A DM9-containing protein from oyster Crassostrea gigas (CgDM9CP-3) mediating immune recognition and encapsulation.

    abstract::DM9 domain containing protein (DM9CP) is a recently identified pattern recognition molecules exiting in most organisms except plants. In the present study, a novel DM9-containing protein (CgDM9CP-3) was identified from Pacific oyster Crassostrea gigas with an open reading frame of 438 bp, encoding a polypeptide of 145...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2020.103937

    authors: Liu Y,Wang W,Zhao Q,Yuan P,Li J,Song X,Liu Z,Ding D,Wang L,Song L

    更新日期:2021-03-01 00:00:00

  • Drosophila melanogaster prophenoloxidases respond inconsistently to Cu2+ and have different activity in vitro.

    abstract::Dipteran insects, like mosquitoes, possess more than two prophenoloxidase (PPO) genes, but it is unclear whether their gene products differ in biochemical properties and physiological functions. Here, we used three Drosophila melanogaster PPOs as models to study their properties through expression in S2 cells. Our dat...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2011.12.001

    authors: Liu F,Chen Y,Yang B,Wang J,Peng Q,Shao Q,Li X,Beerntsen BT,Xu Y,Li J,Yu XQ,Ling E

    更新日期:2012-03-01 00:00:00

  • The M2 macrophages induce autophagic vascular disorder and promote mouse sensitivity to urethane-related lung carcinogenesis.

    abstract::Tumor vessels are known to be abnormal, with typically aberrant, leaky and disordered vessels. Here, we investigated whether polarized macrophage phenotypes are involved in tumor abnormal angiogenesis and what is its mechanism. We found that there was no difference in chemotaxis of polarized M1 and M2 macrophages to l...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2016.01.010

    authors: Li GG,Guo ZZ,Ma XF,Cao N,Geng SN,Zheng YQ,Meng MJ,Lin HH,Han G,Du GJ

    更新日期:2016-06-01 00:00:00

  • In situ lymphocyte apoptosis in larval Xenopus laevis, the South African clawed toad.

    abstract::During Anuran metamorphosis larval structures regress, adult structures form anew and impaired T cell immune functions are noted, as are alterations in endogenous glucocorticoid titers. In situ histological data, after staining for DNA fragmentation, reveal patterns of lymphocyte suicide in the thymus and spleen of no...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/s0145-305x(97)00057-8

    authors: Grant P,Clothier RH,Johnson RO,Ruben LN

    更新日期:1998-07-01 00:00:00

  • The Toll immune signaling pathway control conserved anti-dengue defenses across diverse Ae. aegypti strains and against multiple dengue virus serotypes.

    abstract::Dengue virus has become one of the most important arboviral pathogens affecting the world today. The virus is transmitted among humans by the mosquitoes Aedes aegypti and Ae. albopictus. Like other vector-borne pathogens, this virus encounters innate immune defenses within the mosquito vector that limit infection. We ...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2010.01.006

    authors: Ramirez JL,Dimopoulos G

    更新日期:2010-06-01 00:00:00

  • Trypanosoma cruzi is lysed by coelomic cytolytic factor-1, an invertebrate analogue of tumor necrosis factor, and induces phenoloxidase activity in the coelomic fluid of Eisenia foetida foetida.

    abstract::A cytolytic protein named Coelomic Cytolytic Factor-1 (CCF-1) was isolated from the coelomic fluid of the earthworm Eisenia foetida foetida. Despite the absence of any gene homology, CCF-1 showed functional analogy with the mammalian cytokine tumour necrosis factor (TNF), particularly based on similar lectin-like acti...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/s0145-305x(01)00048-9

    authors: Olivares Fontt E,Beschin A,Van Dijck E,Vercruysse V,Bilej M,Lucas R,De Baetselier P,Vray B

    更新日期:2002-01-01 00:00:00

  • A different repertoire of Galleria mellonella antimicrobial peptides in larvae challenged with bacteria and fungi.

    abstract::To date, functioning of insect humoral immune response is especially well described in Diptera. The mechanisms of pathogen recognition, activation of signaling pathways and regulation of antimicrobial defense peptide expression are relatively well known. The present paper demonstrates evidence that the immune system o...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2010.06.005

    authors: Mak P,Zdybicka-Barabas A,Cytryńska M

    更新日期:2010-10-01 00:00:00

  • Anopheles gambiae hemocytes exhibit transient states of activation.

    abstract::Hemocytes are crucial players of the mosquito immune system and critically affect transmission of pathogens including malaria parasites. We and others discovered previously that a blood meal is a major immune stimulus for mosquito hemocytes. To determine whether these blood meal-induced hemocyte changes in Anopheles g...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2015.10.020

    authors: Bryant WB,Michel K

    更新日期:2016-02-01 00:00:00

  • A novel junctional adhesion molecule A (CgJAM-A-L) from oyster (Crassostrea gigas) functions as pattern recognition receptor and opsonin.

    abstract::Junctional adhesion molecule (JAM), a subfamily of immunoglobulin superfamily (IgSF) with a couple of immunoglobulin domains, can act as regulator in homeostasis and inflammation of vertebrates. In the present study, a structural homolog of JAM-A (designated CgJAM-A-L) was screened out from oyster, Crassostrea gigas, ...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2015.09.011

    authors: Liu C,Wang M,Jiang S,Wang L,Chen H,Liu Z,Qiu L,Song L

    更新日期:2016-02-01 00:00:00

  • An alternative internal splicing site defines new Ikaros isoforms in Pleurodeles waltl.

    abstract::The Ikaros gene encodes a family of transcription factors which plays a crucial role in hematopoiesis. To improve our knowledge about the immune system of Pleurodeles waltl, we sequenced the cDNA coding for the Ik-1 isoform of that salamander and analyzed its tissue expression by semi-quantitative RT-PCR. Ikaros trans...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/s0145-305x(02)00014-9

    authors: Boudarra N,Frippiat C,Dournon C,Frippiat JP

    更新日期:2002-09-01 00:00:00

  • Molecular cloning and expression analysis of mannose receptor C type 1 in grass carp (Ctenopharyngodon idella).

    abstract::Mannose receptor C type 1 (MRC1) is a pattern-recognition receptor (PRR) which plays a significant role in immune responses. Much work on MRC1 has been done in mammals and birds while little in fish. In this study, we cloned and characterized MRC1 in grass carp (gcMR). The full-length gcMR contained 5291bp encoding a ...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2013.10.006

    authors: Wang L,Liu L,Zhou Y,Zhao X,Xi M,Wei S,Fang R,Ji W,Chen N,Gu Z,Liu X,Wang W,Asim M,Liu X,Lin L

    更新日期:2014-03-01 00:00:00

  • Humoral immunoglobulins of the white sturgeon, Acipenser transmontanus: partial characterization of and recognition with monoclonal antibodies.

    abstract::White sturgeon (Acipenser transmontanus) immunoglobulin (Ig) was purified from serum by two methods, ion-exchange chromatography and gel filtration and precipitation of the euglobulin fraction. The purity of these immunoglobulin preparations was confirmed by gel electrophoresis. Sequence analysis of the N-terminal ami...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/0145-305x(96)00015-8

    authors: Adkison MA,Basurco B,Hedrick RP

    更新日期:1996-07-01 00:00:00

  • Different sensitivity to the dexamethasone treatment of the lymphoid organs of Rana perezi in two different seasons.

    abstract::Adult female frogs (Rana perezi) have been used to analyze structural and morphometrical changes which affect lymphoid organs after treatment with a single dose of dexamethasone (DX). Special attention was given to the distinct sensitivity observed during two different periods of the year. Morphological differences we...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/0145-305x(89)90017-7

    authors: Garrido E,Gomariz RP,Leceta J,Zapata A

    更新日期:1989-01-01 00:00:00