Abstract:
:The present study was designed to investigate the effects of start codon of nosM on the biosynthesis of nosiheptide. Target genes were amplified by overlap PCR. After homologous recombination to construct engineered strains, nosiheptide production was analyzed by HPLC. Three mutants with different start codon of nosM were constructed, and nosiheptide production of each mutant was analyzed and compared. Replacement of the start codon of nosM significantly decreased the production of nosiheptide. In conclusion, start codon usage could greatly affect the biosynthetic efficiency in the biosynthetic gene cluster of nosiheptide.
journal_name
Chin J Nat Medjournal_title
Chinese journal of natural medicinesauthors
Jiang L,Xue YJ,Liu WY,Ma M,Wu XR,Wang SZ,Chen YJdoi
10.1016/S1875-5364(15)30089-3subject
Has Abstractpub_date
2015-11-01 00:00:00pages
854-860issue
11eissn
2095-6975issn
1875-5364pii
S1875-5364(15)30089-3journal_volume
13pub_type
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