Abstract:
:In our previous study, we have elucidated the chemical profile of YGS40, a fraction of Yi-Gan San (YGS), used for the treatment of Alzheimer's disease (AD). Oxidative stress-induced apoptosis is implicated in neurodegenerative disorders such as AD. The aim of the present study was to explore the protective effects of YGS40 against hydrogen peroxide (H2O2)-induced apoptosis in PC12 cells and the underlying mechanisms. PC12 cells were exposed to 100 μmol·L(-1) of H2O2 for 12 h with or without YGS40 pretreatment. Cytotoxicity was determined by MTT (3, (4, 5-dimethylthiazole-2-yl) 2, 5-diphenyl-tetrazolium bromide) and lactate dehydrogenase (LDH) release assays; apoptosis was detected by Annexin V/propidium iodide coupled staining and by determining caspase-3 activity and Bax and Bcl-2 protein levels. Mitochondrial membrane potential (MMP) was assessed by the retention of rhodamine123; and the activities of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured using commercially available enzymatic kits. Pretreatment with YGS40 significantly prevented H2O2-induced cytotoxicity and protected the cells against H2O2-triggered apoptosis characterized by externalization of membrane phosphatidylserine and caspase-3 activation and the increased ratio of Bax/Bcl-2 in PC12 cells. Further studies showed that YGS40 suppressed H2O2-induced MMP loss, increased SOD activity, and decreased MDA level. These findings suggest that YGS40 may be beneficial for the prevention and treatment of oxidative stress-mediated disorders.
journal_name
Chin J Nat Medjournal_title
Chinese journal of natural medicinesauthors
Zhao YR,Qu W,Liu WY,Hong H,Feng F,Chen H,Xie Ndoi
10.1016/S1875-5364(15)30037-6subject
Has Abstractpub_date
2015-06-01 00:00:00pages
438-44issue
6eissn
2095-6975issn
1875-5364pii
S1875-5364(15)30037-6journal_volume
13pub_type
杂志文章abstract:AIM:To study the chemical constituents of the solid culture of the endophyte Phomopsis sp. IFB-E060 in Vatica mangachapoi. METHOD:Isolation and purification were performed through silica gel column chromatography, gel filtration over Sephadex LH-20, ODS column chromatography, and HPLC. Structures of the isolated compo...
journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
doi:10.1016/S1875-5364(14)60080-7
更新日期:2014-07-01 00:00:00
abstract::Novel series of limonin derivatives (V-A-1-V-A-8, V-B-1-V-B-8) were synthesized by adding various tertiary amines onto the C (7)-position of limonin. The synthesized compounds possessed favorable physicochemical property, and the intrinsic solubility of the novel compounds were significantly improved, compared with li...
journal_title:Chinese journal of natural medicines
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doi:10.1016/S1875-5364(18)30052-9
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abstract::Panax notoginseng saponins (PNS) are the major components of Panax notoginseng, with multiple pharmacological activities but poor oral bioavailability. PNS could be metabolized by gut microbiota in vitro, while the exact role of gut microbiota of PNS metabolism in vivo remains poorly understood. In this study, pseudo ...
journal_title:Chinese journal of natural medicines
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doi:10.1016/S1875-5364(19)30026-3
更新日期:2019-03-01 00:00:00
abstract:AIM:To study the chemical constituents of the fruits of Illicium henryi. METHOD:Chromatographic separations on silica gel, Sephadex LH-20 gel and MCI gel were used to isolate the compounds. The structures were elucidated based on extensive spectroscopic data analyses. RESULTS:Seven compounds were obtained and their s...
journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
doi:10.1016/S1875-5364(14)60074-1
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abstract::The constituents of Cimicifuga plants have been extensively investigated, and the principal metabolites are 9,19-cyclolanostane triterpenoid glycosides, which are distributed widely in Cimicifuga plants, but not in other members of the Ranunculaceae family, and are considered to be characteristics of the Cimicifuga ge...
journal_title:Chinese journal of natural medicines
pub_type: 杂志文章,评审
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
doi:10.1016/S1875-5364(14)60086-8
更新日期:2014-08-01 00:00:00
abstract::Hybanthus enneaspermus (L.) F. Muell belonging to the family Violaceae, popularly known as Ratanpurus (Hindi) is a herb or a shrub distributed in the tropical and subtropical regions of the world. In the Ayurvedic literature, the plant is reported to cure conditions of "Kapha" and "Pitta", urinary calculi, strangury, ...
journal_title:Chinese journal of natural medicines
pub_type: 杂志文章,评审
doi:10.1016/S1875-5364(13)60017-5
更新日期:2013-05-01 00:00:00
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
doi:10.1016/S1875-5364(17)30025-0
更新日期:2017-02-01 00:00:00
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章,评审
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更新日期:2019-11-01 00:00:00
abstract::Garcinia, a kind of dry resin secreted by Garcinia hanburyi Hook. F. G., is a traditional Chinese medicine with various biological functions such as detoxification, anti-inflammatory, and anthelmintic activities. Recent studies suggest that garcinia has potential anticancer activity. Increasing evidences indicate that...
journal_title:Chinese journal of natural medicines
pub_type: 杂志文章,评审
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更新日期:2018-09-01 00:00:00
abstract:AIM:To investigate the effects of feeding phenylalanine (Phe) and tyrosine (Tyr) on the accumulation of total phenolic compounds and four phenylethanoid glycosides (PeGs) to a cell suspension culture of the parasitic plant Cistanche deserticola. METHOD:A cell suspension culture of C. deserticola was established and pr...
journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
doi:10.1016/S1875-5364(14)60045-5
更新日期:2014-05-01 00:00:00
abstract:AIM:To study the chemical constituents and bioactivity of the stem bark of Mitragyna diversifolia. METHOD:Compounds were isolated by various chromatographic methods. Their structures were elucidated on the basis of spectroscopic techniques (IR, UV, MS, and NMR), and they were evaluated for their cytotoxic activities b...
journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
doi:10.1016/S1875-5364(14)60096-0
更新日期:2014-08-01 00:00:00
abstract::Diosgenin, a well-known steroid sapogenin derived from plants, has been used as a starting material for production of steroidal hormones. The present review will summarize published literature concerning pharmacological potential of diosgenin, and the underlying mechanisms of actions. Diosgenin has shown a vast range ...
journal_title:Chinese journal of natural medicines
pub_type: 杂志文章,评审
doi:10.1016/S1875-5364(15)30053-4
更新日期:2015-08-01 00:00:00
abstract::The saponin ginsenoside Rk1 is a major compound isolated from ginseng. Ginsenoside Rk1 has been reported to have anti-inflammatory and anti-tumor properties and to be involved in the regulation of metabolism. However, the effect and mechanism of anti-inflammatory action of ginsenoside Rk1 has not been fully clarified....
journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
doi:10.1016/S1875-5364(17)30106-1
更新日期:2017-10-01 00:00:00
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
doi:10.1016/S1875-5364(16)30077-2
更新日期:2016-09-01 00:00:00
abstract:AIM:To study the chemical constituents of the aerial parts of Cirsium setosum (Willd.) MB.. METHODS:The chemical constituents were isolated and purified by various chromatographic techniques. Their structures were determined on the basis of physical properties and spectroscopic data. RESULTS:A new megastigmane glycos...
journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
doi:10.1016/S1875-5364(13)60097-7
更新日期:2013-09-01 00:00:00
abstract::Polysaccharide from traditional Chinese herb, Saposhnikovia divaricata (Turcz.) Schischk. (SD) was extracted, fractionated and characterized in this work. Four fractions were prepared. Their molecular weight, monosaccharide compositions, linkage modes and structural properties were characterized with SEC-MALS-RI, HPAE...
journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
doi:10.1016/S1875-5364(19)30096-2
更新日期:2019-10-01 00:00:00
abstract::Atractylenolide III (ATL-III), a sesquiterpene compound isolated from Rhizoma Atractylodis Macrocephalae, has revealed a number of pharmacological properties including anti-inflammatory, anti-cancer activity, and neuroprotective effect. This study aimed to evaluate the cytoprotective efficiency and potential mechanism...
journal_title:Chinese journal of natural medicines
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
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更新日期:2016-06-01 00:00:00
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
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更新日期:2018-07-01 00:00:00
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
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更新日期:2014-02-01 00:00:00
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
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更新日期:2013-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2019-07-01 00:00:00
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journal_title:Chinese journal of natural medicines
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journal_title:Chinese journal of natural medicines
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
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更新日期:2016-02-01 00:00:00
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
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更新日期:2018-04-01 00:00:00
abstract::Penicildiones A-D (1-4), four new steroids derivatives together with three known compounds including 16α-methylpregna-17α,19-dihydroxy-(9,11)-epoxy-4-ene-3,18-dione-20-acetoxy (5), stachybotrylactone B (6) and stachybotrin (7) were isolated from the soft coral-derived fungus Penicillium sp. SCSIO41201, cultured in the...
journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
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更新日期:2020-04-01 00:00:00