Abstract:
:Several chemical compounds can restore pigmentation in vitiligo through mechanisms that vary according to disease etiology. In the present study, we investigated the melanogenic activity of six structurally distinct compounds, namely, scopoletin, kaempferol, chrysin, vitamin D3, piperine, and 6-benzylaminopurine. We determined their effectiveness, toxicity, and mechanism of action for stimulating pigmentation in B16F10 melanoma cells and in a zebrafish model. The melanogenic activity of 6-benzylaminopurine, the compound identified as the most potent, was further verified by measuring green fluorescent protein concentration in tyrp1 a: eGFP (tyrosinase-related protein 1) zebrafish and mitfa: eGFP (microphthalmia associated transcription factor) zebrafish and antioxidative activity. All the tested compounds were found to enhance melanogenesis responses both in vivo and in vitro at their respective optimal concentration by increasing melanin content and expression of TYR and MITF. 6-Benzyamino-purine showed the strongest re-pigmentation action at a concentration of 20 μmol·L-1in vivo and 100 μmol·L-1in vitro, and up-regulated the strong fluorescence expression of green fluorescent protein in tyrp1a: eGFP and mitfa: eGFP zebrafish in vitro. However, its relative anti-oxidative activity was found to be very low. Overall, our results indicated that 6-benzylaminopurine stimulated pigmentation through a direct mechanism, by increasing melanin content via positive regulation of tyrosinase activity in vitro, as well as up-regulating the expression of the green fluorescent protein in transgenic zebrafish in vivo.
journal_name
Chin J Nat Medjournal_title
Chinese journal of natural medicinesauthors
Heriniaina RM,Dong J,Kalavagunta PK,Wu HL,Yan DS,Shang Jdoi
10.1016/S1875-5364(18)30116-Xsubject
Has Abstractpub_date
2018-10-01 00:00:00pages
766-773issue
10eissn
2095-6975issn
1875-5364pii
S1875-5364(18)30116-Xjournal_volume
16pub_type
杂志文章abstract::Atractylenolide III (ATL-III), a sesquiterpene compound isolated from Rhizoma Atractylodis Macrocephalae, has revealed a number of pharmacological properties including anti-inflammatory, anti-cancer activity, and neuroprotective effect. This study aimed to evaluate the cytoprotective efficiency and potential mechanism...
journal_title:Chinese journal of natural medicines
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journal_title:Chinese journal of natural medicines
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journal_title:Chinese journal of natural medicines
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journal_title:Chinese journal of natural medicines
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journal_title:Chinese journal of natural medicines
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journal_title:Chinese journal of natural medicines
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journal_title:Chinese journal of natural medicines
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journal_title:Chinese journal of natural medicines
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章,评审
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journal_title:Chinese journal of natural medicines
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
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journal_title:Chinese journal of natural medicines
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journal_title:Chinese journal of natural medicines
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章,评审
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
doi:10.1016/S1875-5364(15)30080-7
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章,评审
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
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journal_title:Chinese journal of natural medicines
pub_type: 历史文章,杂志文章,评审
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
doi:10.1016/S1875-5364(14)60074-1
更新日期:2014-06-01 00:00:00
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journal_title:Chinese journal of natural medicines
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
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journal_title:Chinese journal of natural medicines
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
doi:10.3724/SP.J.1009.2015.00073
更新日期:2016-01-01 00:00:00
abstract::Lonicerae Japonicae Flos (LJF), a kind of traditional Chinese medicines (TCMs), has functions of detoxifying and evacuating heat. In the study, a method based on ultra-high performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap MS) was developed for the chemical c...
journal_title:Chinese journal of natural medicines
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更新日期:2020-02-01 00:00:00
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
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更新日期:2015-06-01 00:00:00
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journal_title:Chinese journal of natural medicines
pub_type: 杂志文章
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更新日期:2016-10-01 00:00:00