Screening and analysis of key active constituents in Guanxinshutong capsule using mass spectrum and integrative network pharmacology.

Abstract:

:Guanxinshutong capsule (GXSTC) is an effective and safe traditional Chinese medicine used in the treatment of cardiovascular diseases (CVDs) for many years. However, the targets of this herbal formula and the underlying molecular mechanisms of action involved in the treatment of CVDs are still unclear. In the present study, we used a systems pharmacology approach to identify the active ingredients of GXSTC and their corresponding targets in the calcium signaling pathway with respect to the treatment of CVDs. This method integrated chromatographic techniques, prediction of absorption, distribution, metabolism, and excretion, analysis using Kyoto Encyclopedia of Genes and Genomes, network construction, and pharmacological experiments. 12 active compounds and 33 targets were found to have a role in the treatment of CVDs, and four main active ingredients, including protocatechuic acid, cryptotanshinone, eugenol, and borneol were selected to verify the effect of (GXSTC) on calcium signaling system in cardiomyocyte injury induced by hypoxia and reoxygenation. The results from the present study revealed the active components and targets of GXSTC in the treatment of CVDs, providing a new perspective to enhance the understanding of the role of the calcium signaling pathway in the therapeutic effect of GXSTC.

journal_name

Chin J Nat Med

authors

Liu F,DU X,Liu PR,Sun YH,Zhang YM

doi

10.1016/S1875-5364(18)30060-8

subject

Has Abstract

pub_date

2018-04-01 00:00:00

pages

302-312

issue

4

eissn

2095-6975

issn

1875-5364

pii

S1875-5364(18)30060-8

journal_volume

16

pub_type

杂志文章
  • Difficulties in research of Chinese medicine polysaccharides.

    abstract::Polysaccharides from Chinese medicines are attracting increasing attention to their wide range of valuable biological activities. As these polysaccharides are mostly from edible materials, their safety can be greatly ensured. Therefore, the Chinese medicine polysaccharides have been the focus of research and developme...

    journal_title:Chinese journal of natural medicines

    pub_type: 社论

    doi:10.1016/S1875-5364(19)30107-4

    authors: Li LF,Wong TL,Han QB

    更新日期:2019-12-01 00:00:00

  • Discovery of novel limonin derivatives as potent anti-inflammatory and analgesic agents.

    abstract::Novel series of limonin derivatives (V-A-1-V-A-8, V-B-1-V-B-8) were synthesized by adding various tertiary amines onto the C (7)-position of limonin. The synthesized compounds possessed favorable physicochemical property, and the intrinsic solubility of the novel compounds were significantly improved, compared with li...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(18)30052-9

    authors: Wang SC,Yang Y,Liu J,Jiang AD,Chu ZX,Chen SY,Gong GQ,He GW,Xu YG,Zhu QH

    更新日期:2018-03-01 00:00:00

  • Two new ursane-type nortriterpenes from Lonicera macranthoides and their iNOS-inhibitory activities.

    abstract::The flower buds of Lonicera macranthoides (Shan Yin-Hua), represent an important traditional Chinese medicine and food ingredient. A phytochemical investigation of the 70% EtOH extract of the flower buds of L. macranthoides resulted in the isolation of 12 triterpenoids (1-12), including two new ursane-type nortriterpe...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(19)30006-8

    authors: Mei YD,Zhang N,Zhang WY,Tang JS,Zhou H,Yu Y,Yao XS

    更新日期:2019-01-01 00:00:00

  • Traditional Chinese Medicine: an effective treatment for 2019 novel coronavirus pneumonia (NCP).

    abstract::The novel coronavirus pneumonia broke out in 2019 and spread rapidly. In 30 different countries, there are over seventy thousand patients have been diagnosed in total. Therefore, it is urgent to develop the effective program to prevent and treat for the novel coronavirus pneumonia. In view of Traditional Chinese Medic...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章,评审

    doi:10.1016/S1875-5364(20)30022-4

    authors: DU HZ,Hou XY,Miao YH,Huang BS,Liu DH

    更新日期:2020-03-01 00:00:00

  • Maslinic acid modulates glycogen metabolism by enhancing the insulin signaling pathway and inhibiting glycogen phosphorylase.

    abstract:AIM:To investigate the molecular signaling mechanism by which the plant-derived, pentacyclic triterpene maslinic acid (MA) exerts anti-diabetic effects. METHOD:HepG2 cells were stimulated with various concentrations of MA. The effects of MA on glycogen phosphorylase a (GPa) activity and the cellular glycogen content w...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(14)60052-2

    authors: Liu J,Wang X,Chen YP,Mao LF,Shang J,Sun HB,Zhang LY

    更新日期:2014-04-01 00:00:00

  • Modified Da-chai-hu Decoction regulates the expression of occludin and NF-κB to alleviate organ injury in severe acute pancreatitis rats.

    abstract::Modified Da-chai-hu Decoction (MDD), a traditional Chinese medicinal formulation, which was empirically generated from Da-chai-hu decoction, has been utilized to treat severe acute pancreatitis (SAP) for decades. The aim of the present study was to explore its potential organprotective mechanism in SAP. In the present...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(19)30041-X

    authors: Zhao G,Zhuo YZ,Cui LH,Li CX,Chen SY,Li D,Liu JH,Li DH,Cui NQ,Zhang SK

    更新日期:2019-05-20 00:00:00

  • Radioprotective effect of adenine on irradiation-induced apoptosis.

    abstract:AIM:To investigate the radioprotective effect of adenine on irradiated lymphocytes and discover the possible mechanisms of protection. METHODS:Lymphocytes were pretreated for 12 h with adenine (0.001-0.1 μmol·L(-1)) and then exposed to 4 Gy radiation. Cell viability was observed by the MTS assay, apoptosis was detecte...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(13)60040-0

    authors: Wang XY,Ma ZC,Shao S,Hong Q,Wang YG,Tan HL,Lu XQ,Dong Z,Gao Y

    更新日期:2013-03-01 00:00:00

  • Hybanthus enneaspermus (L.) F. Muell: a concise report on its phytopharmacological aspects.

    abstract::Hybanthus enneaspermus (L.) F. Muell belonging to the family Violaceae, popularly known as Ratanpurus (Hindi) is a herb or a shrub distributed in the tropical and subtropical regions of the world. In the Ayurvedic literature, the plant is reported to cure conditions of "Kapha" and "Pitta", urinary calculi, strangury, ...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章,评审

    doi:10.1016/S1875-5364(13)60017-5

    authors: Patel DK,Kumar R,Sairam K,Hemalatha S

    更新日期:2013-05-01 00:00:00

  • Oral JS-38, a metabolite from Xenorhabdus sp., has both anti-tumor activity and the ability to elevate peripheral neutrophils.

    abstract:AIM:JS-38 (mitothiolore), a synthetic version of a metabolite isolated from Xenorhabdus sp., was evaluated for its anti-tumor and white blood cell (WBC) elevating activities. METHOD:These anti-proliferative activities were assessed in vitro using a panel of ten cell lines. The anti-tumor activities were tested in vivo...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(14)60117-5

    authors: Liu MY,Xiao L,Chen GH,Wang YX,Xiong WX,Li F,Liu Y,Huang XL,Deng YF,Zhang Z,Sun HY,Liu QH,Yin M

    更新日期:2014-10-01 00:00:00

  • Chemical profiling of Qixue Shuangbu Tincture by ultra-performance liquid chromatography with electrospray ionization quadrupole-time-of-flight high-definition mass spectrometry (UPLC-QTOF/MS).

    abstract::The present study was designed to develop and validate a sensitive and reliable ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) method to separate and identify the chemical constituents of Qixue Shuangbu Tincture (QXSBT), a classic traditional Chines...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(16)60007-9

    authors: Chen LW,Wang Q,Qin KM,Wang XL,Wang B,Chen DN,Cai BC,Cai T

    更新日期:2016-02-01 00:00:00

  • Total synthesis of D-glycero-D-mannno-heptose 1β, 7-bisphosphate with 3-O-amyl amine linker and its monophosphate derivative.

    abstract::D-Glycero-D-mannno-heptose 1β, 7-bisphosphate (HBPβ) is an important intermediate for constructing the core structure of Gram-negative bacterial lipopolysaccharides and was reported as a pathogen-associated molecular pattern (PAMP) that regulates immune responses. HBPβ with 3-O-amyl amine linker and its monophosphate ...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(20)30075-3

    authors: Zou XP,Qin CJ,Hu J,Fu JJ,Tian GZ,Moscovitz O,Seeberger PH,Yin J

    更新日期:2020-08-01 00:00:00

  • Two new steroidal saponins isolated from Anemarrhena asphodeloides.

    abstract::Two new steroidal saponins, named timosaponin P (1) and timosaponin Q (2), were isolated from the rhizome parts of Anemarrhena asphodeloides Bunge using various chromatographic methods. Their structures and absolute configurations were elucidated by a combination of spectroscopic and spectrometric data, including 1D, ...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(17)30038-9

    authors: Sun XH,Zhu FT,Zhang YW,Chen FF,Yu Y,Song NN,Huang XF

    更新日期:2017-03-01 00:00:00

  • Two novel resin glycosides isolated from Ipomoea cairica with α-glucosidase inhibitory activity.

    abstract::In the present study, two new compounds from Ipomoea cairica were identified and demonstrated to have α-glucosidase inhibitory activity. They were isolated by column chromatography on silica gel and sephadex LH-20 and finally purified by prep-HPLC, with their structures being elucidated by spectroscopic methods, such ...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(16)30020-6

    authors: Li JH,Pan JT,Yin YQ

    更新日期:2016-03-01 00:00:00

  • Quality control of traditional Chinese medicines: a review.

    abstract::Traditional Chinese medicines (TCMs) are in great demand all over the world, especially in the developing world, for primary health care due to their superior merits such as low cost, minimal side effects, better cultural acceptability, and compatibility with humans. However, Chinese medicines consist of several herbs...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章,评审

    doi:10.1016/S1875-5364(13)60069-2

    authors: Song XY,Li YD,Shi YP,Jin L,Chen J

    更新日期:2013-11-01 00:00:00

  • Comprehensive analysis of the chemical constituents in sulfur-fumigated Lonicerae Japonicae Flos using UHPLC-LTQ-Orbitrap mass spectrometry.

    abstract::Lonicerae Japonicae Flos (LJF), a kind of traditional Chinese medicines (TCMs), has functions of detoxifying and evacuating heat. In the study, a method based on ultra-high performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap MS) was developed for the chemical c...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(20)30015-7

    authors: Liu ZH,Wang YQ,Mei XD,Wang F,Yang XZ,Li XD,Jiang F,Zhang JY

    更新日期:2020-02-01 00:00:00

  • Transformation of astragalosides from radix astragali under acidic, neutral, and alkaline extraction conditions monitored by LC-ESI-TOF/MS.

    abstract:AIM:To reveal the profile of astragalosides for better quality evaluation of Radix Astragali, this study was aimed to investigate the transformation of astragalosides under different conditions. METHOD:Seven major astragalosides were selected for evaluation under acidic, neutral and alkaline conditions. The transforma...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(14)60062-5

    authors: Chu C,Liu EH,Qi LW,Li P

    更新日期:2014-04-01 00:00:00

  • An in vitro study on interaction of anisodine and monocrotaline with organic cation transporters of the SLC22 and SLC47 families.

    abstract::Current study systematically investigated the interaction of two alkaloids, anisodine and monocrotaline, with organic cation transporter OCT1, 2, 3, MATE1 and MATE2-K by using in vitro stably transfected HEK293 cells. Both anisodine and monocrotaline inhibited the OCTs and MATE transporters. The lowest IC50 was 12.9 µ...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(19)30070-6

    authors: Chen JY,Brockmöller J,Tzvetkov MV,Wang LJ,Chen XJ

    更新日期:2019-07-01 00:00:00

  • Triterpenes from the stem bark of Mitragyna diversifolia and their cytotoxic activity.

    abstract:AIM:To study the chemical constituents and bioactivity of the stem bark of Mitragyna diversifolia. METHOD:Compounds were isolated by various chromatographic methods. Their structures were elucidated on the basis of spectroscopic techniques (IR, UV, MS, and NMR), and they were evaluated for their cytotoxic activities b...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(14)60096-0

    authors: Cao XF,Wang JS,Wang PR,Kong LY

    更新日期:2014-08-01 00:00:00

  • Involvement of mitochondrial apoptotic pathway and MAPKs/NF-κ B inflammatory pathway in the neuroprotective effect of atractylenolide III in corticosterone-induced PC12 cells.

    abstract::Atractylenolide III (ATL-III), a sesquiterpene compound isolated from Rhizoma Atractylodis Macrocephalae, has revealed a number of pharmacological properties including anti-inflammatory, anti-cancer activity, and neuroprotective effect. This study aimed to evaluate the cytoprotective efficiency and potential mechanism...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(19)30030-5

    authors: Gong WX,Zhou YZ,Qin XM,DU GH

    更新日期:2019-04-01 00:00:00

  • Characterization and evaluation in vivo of baicalin-nanocrystals prepared by an ultrasonic-homogenization-fluid bed drying method.

    abstract:AIM:To improve the absorption and bioavailability of baicalin using a nanocrystal (or nanosuspension) drug delivery system. METHODS:A tandem, ultrasonic-homogenization-fluid bed drying technology was applied to prepare baicalin-nanocrystal dried powders, and the physicochemical properties of baicalin-nanocrystals were...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(14)60012-1

    authors: Shi-Ying J,Jin H,Shi-Xiao J,Qing-Yuan L,Jin-Xia B,Chen HG,Rui-Sheng L,Wei W,Hai-Long Y

    更新日期:2014-01-01 00:00:00

  • Effects of six compounds with different chemical structures on melanogenesis.

    abstract::Several chemical compounds can restore pigmentation in vitiligo through mechanisms that vary according to disease etiology. In the present study, we investigated the melanogenic activity of six structurally distinct compounds, namely, scopoletin, kaempferol, chrysin, vitamin D3, piperine, and 6-benzylaminopurine. We d...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(18)30116-X

    authors: Heriniaina RM,Dong J,Kalavagunta PK,Wu HL,Yan DS,Shang J

    更新日期:2018-10-01 00:00:00

  • 17-[16,17-Dihydroxycyclooctatinyl]-hexaketide ester from Streptomyces sp. SR107.

    abstract::A new hexaketide acid esterified by the 17-hydroxyl group of 16,17-dihydroxycyclooctatin, namely 17-[16,17-dihydroxycyclooctatinyl]-hexaketide ester (1), a member of the group of rare bacterial diterpenes with a fused 5-8-5 ring system was isolated from strain Streptomyces sp. SR107. The structure was determined on th...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(17)30018-3

    authors: Liu MY,Li SR,Chun-Hua LU

    更新日期:2016-12-01 00:00:00

  • Total synthesis of neokotalanol, a potent α-glucosidase inhibitor isolated from Salacia reticulata.

    abstract::Neokotalanol, a potent α-glucosidase inhibitor isolated from Salacia reticulata, was synthesized through a key coupling reaction between a perbenzylated thiosugar and an appropriately protected perseitol triflate derived from D-mannose. This key step was found to be quite temperature dependent, and a simultaneous cycl...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(13)60079-5

    authors: Xie WJ,Tanabe G,Tsutsui N,Wu XM,Muraoka O

    更新日期:2013-11-01 00:00:00

  • A new quinoline alkaloid from the roots of Dictamnus angustifolius.

    abstract:AIM:To investigate the quinoline alkaloids from the roots of Dictamnus angustifolius G.Don ex Sweet (Rutaceae). METHOD:The quinoline alkaloids were isolated by various column chromatographic methods and their structures were elucidated on the basis of spectral analysis. RESULTS:A new quinoline alkaloid, 5-methoxylrob...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(14)60037-6

    authors: Sun JB,Qu W,Guan FQ,Li LZ,Liang JY

    更新日期:2014-03-01 00:00:00

  • Combination of LC/MS and GC/MS based metabolomics to study the hepatotoxic effect of realgar nanoparticles in rats.

    abstract::Realgar nanoparticles (NPs) are increasingly used as therapeutic agents for their enhanced anti-proliferation effect and cytotoxicity on cancer cells. However, the alteration of particle size may enhance biological reactivity as well as toxicity. A LC/MS and GC/MS based metabolomics approach was employed to explore th...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(17)30098-5

    authors: Zhang MH,Chen JQ,Guo HM,Li RT,Gao YQ,Tian Y,Zhang ZJ,Huang Y

    更新日期:2017-09-01 00:00:00

  • Advances in the pharmacological activities and mechanisms of diosgenin.

    abstract::Diosgenin, a well-known steroid sapogenin derived from plants, has been used as a starting material for production of steroidal hormones. The present review will summarize published literature concerning pharmacological potential of diosgenin, and the underlying mechanisms of actions. Diosgenin has shown a vast range ...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章,评审

    doi:10.1016/S1875-5364(15)30053-4

    authors: Chen Y,Tang YM,Yu SL,Han YW,Kou JP,Liu BL,Yu BY

    更新日期:2015-08-01 00:00:00

  • Siwu decoction attenuates oxonate-induced hyperuricemia and kidney inflammation in mice.

    abstract::The aim of the study was to investigate the effects of Siwu decoction on hyperuricemia, kidney inflammation, and dysfunction in hyperuricemic mice. Siwu decoction at 363.8, 727.5, and 1 455 mg·kg(-1) was orally administered to potassium oxonate-induced hyperuricemic mice for 7 days. Serum urate, creatinine, and blood ...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(16)30059-0

    authors: Wang R,Ma CH,Zhou F,Kong LD

    更新日期:2016-07-01 00:00:00

  • YGS40, an active fraction of Yi-Gan San, reduces hydrogen peroxide-induced apoptosis in PC12 cells.

    abstract::In our previous study, we have elucidated the chemical profile of YGS40, a fraction of Yi-Gan San (YGS), used for the treatment of Alzheimer's disease (AD). Oxidative stress-induced apoptosis is implicated in neurodegenerative disorders such as AD. The aim of the present study was to explore the protective effects of ...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(15)30037-6

    authors: Zhao YR,Qu W,Liu WY,Hong H,Feng F,Chen H,Xie N

    更新日期:2015-06-01 00:00:00

  • Effects of Bunao-Fuyuan decoction serum on proliferation and migration of vascular smooth muscle cells in atherosclerotic.

    abstract::Atherosclerosis (AS) is a chronic inflammatory disease, the main causes of which include abnormal lipid metabolism, endothelial injury, physical and chemical injury, hemodynamic injury, genetic factors and so on. These causes can lead to inflammatory injury of blood vessels and local dysfunction. Bunao-Fuyuan decoctio...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(21)60004-3

    authors: Guo HY,Lu ZY,Zhao B,Jiang WW,Xiong YH,Wang K

    更新日期:2021-01-01 00:00:00

  • Isochlorogenic acid A affects P450 and UGT enzymes in vitro and in vivo.

    abstract::Isochlorogenic acid A (ICQA), which has anti-inflammatory, hepatoprotective, and antiviral properties, is commonly presented in fruits, vegetables, coffee, plant-based food products, and herbal medicines. These herbal medicines are usually used in combination with other medicines in the clinic. However, little is know...

    journal_title:Chinese journal of natural medicines

    pub_type: 杂志文章

    doi:10.1016/S1875-5364(16)30103-0

    authors: Wang J,Wang H,Peng Y,Wang GJ,Hao HP

    更新日期:2016-11-01 00:00:00