Abstract：
:Leukemic cell lines have become important tools for studies of disease providing a monoclonal cell population that can be extensively expanded in vitro while preserving leukemic cellular characteristics. However, studies of chronic lymphocytic leukemia (CLL) have been impeded in part by the lack of continuous human cell lines. CLL cells have a high spontaneous apoptosis rate in vitro and exhibit minimal proliferation in xenograft models. Therefore, there is a need for development of primary CLL cell lines and we describe the isolation of such a line from the bone marrow of a CLL patient (17p deletion and TP53 mutation) which has been in long term culture for more than 12 months with continuous proliferation. The CLL cell line (termed MDA-BM5) which was generated in vitro with continuous co-culture on autologous stromal cells is CD19+CD5+ and shows an identical pattern of somatic hypermutation as determined in the patient's bone marrow (BM), confirming the origin of the cells from the original CLL clone. MDA-BM5 cells were readily transplantable in NOD/SCID gamma null mice (NSG) with disease developing in the BM, liver and spleen. BM cells from quaternary serial transplantation in NSG mice demonstrated the presence of CD19+CD5+ cells with Ig restricted to lambda which is consistent with the original patient cells. These studies describe a new CLL cell line from a patient with del(17p) that provides a unique model for in vitro and in vivo studies.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Kellner J,Wierda W,Shpall E,Keating M,McNiece Idoi
10.1016/j.leukres.2015.10.008subject
Has Abstractpub_date
2016-01-01 00:00:00pages
54-9eissn
0145-2126issn
1873-5835pii
S0145-2126(15)30535-Xjournal_volume
40pub_type
杂志文章abstract：:To evaluate the clinical usefulness of interleukin 2 (IL-2) on myelodysplastic syndromes (MDS), the serum IL-2 level, the effect of IL-2 on the proliferation of blasts, and the cell-mediated cytotoxic effect of IL-2 on blasts were examined in MDS patients. Of 18 patients, 2 patients had an increased serum IL-2 level. ...
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journal_title：Leukemia research
pub_type： 杂志文章,多中心研究
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journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2007.09.014
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pub_type： 杂志文章
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更新日期：1991-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2005-08-01 00:00:00
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pub_type： 杂志文章
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pub_type： 临床试验,杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2010-07-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章,多中心研究
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更新日期：2014-05-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
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更新日期：2005-03-01 00:00:00
