Abstract：
:Expression of cell cycle-regulating genes was studied in human myeloid leukemia cell lines ML-1, ML-2 and ML-3 during induction of differentiation in vitro. Myelomonocytic differentiation was induced by phorbol ester (12-o-Tetradecanoyl-phorbol-13-acetate, TPA), tumor necrosis factor alpha (TNFalpha) or interferon gamma (INFgamma), or their combination. Differentiation (with the exception of TNFalpha alone) was accompanied by inhibition of DNA synthesis and cell cycle arrest. Inhibition of proliferation was associated with a decrease in the expression of cdc25A and cdc25B, cdk6 and Ki-67 genes, and with increased p21(Waf1/Cip1) gene expression, as measured by comparative RT-PCR. Expression of the following genes was not changed after induction of differentiation: cyclin A1, cyclin D3, cyclin E1 and p27(Kip1). Surprisingly, cyclin D1 expression was upregulated after induction by TPA, TNFalpha with IFNgamma or BA. Cyclin D2 was upregulated only after induction by BA. The results of the expression of the tested genes obtained by comparative RT-PCR were confirmed by quantitative real-time (RQ) RT-PCR and Western blotting. Quantitative RT-PCR showed as much as a 288-fold increase of cyclin D1 specific mRNA after a 24h induction by TPA. The upregulation of cyclin D1 in differentiating cells seems to be compensated by the upregulation of p21(Waf1/Cip1). These results, besides others, point to a strong correlation between the expression of cyclin D1 and p21(Waf1/Cip1) on the one hand and differentiation on the other hand in human myeloid leukemic cells and reflect a rather complicated network regulating proliferation and differentiation of leukemic cells.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Ullmannová V,Stöckbauer P,Hradcová M,Soucek J,Haskovec Cdoi
10.1016/s0145-2126(03)00103-6keywords:
subject
Has Abstractpub_date
2003-12-01 00:00:00pages
1115-23issue
12eissn
0145-2126issn
1873-5835pii
S0145212603001036journal_volume
27pub_type
杂志文章abstract：:We report a patient with Philadelphia chromosome positive (Ph +ve) chronic myelogenous leukemia (CML), treated with hydroxyurea alone, who upon disease progression developed an additional Ph - ve clone containing chromosomal abnormalities typical of myelodysplastic syndrome (MDS). Retrospective analysis of a cryoprese...
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journal_title：Leukemia research
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更新日期：2008-09-01 00:00:00
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更新日期：2019-06-01 00:00:00
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更新日期：1993-12-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2003.08.003
更新日期：2004-04-01 00:00:00
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更新日期：1984-01-01 00:00:00
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pub_type： 临床试验,杂志文章
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pub_type： 信件
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更新日期：2006-07-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
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更新日期：2007-05-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
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更新日期：1984-01-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
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更新日期：2012-07-01 00:00:00
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journal_title：Leukemia research
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更新日期：2017-10-01 00:00:00
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journal_title：Leukemia research
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更新日期：1985-01-01 00:00:00
abstract：BACKGROUND/AIMS:Jumping translocations are rare cytogenetic aberrations in haematological malignancies, the pathogenesis of which remains to be fully characterised. We investigated the mechanism of formation of jumping translocations in a case of adult common acute lymphoblastic leukaemia (ALL) positive for the Ph tran...
journal_title：Leukemia research
pub_type： 杂志文章
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更新日期：2004-10-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
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更新日期：2015-03-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
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更新日期：1991-01-01 00:00:00
abstract：:Diagnosis of MDS has changed during the last years because of the 2008 WHO classification. Complete blood cell count (CBC) is a very important tool both for diagnosis of MDS. The aim of this study was to evaluate if it is possible to use the abnormal signals produced by dysplastic cells to produce a flag "dysplasia" a...
journal_title：Leukemia research
pub_type： 杂志文章
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