Abstract：
:Human epidermal keratinocytes were grown in culture and the uptake of hematoporphyrin derivatives (HPDs) used in photodynamic therapy was estimated. Keratinocytes loaded with HPDs were irradiated with laser light of 632 nm generated by a helium-neon laser and cell toxicity was determined by the trypan blue exclusion test and the measurement of enzyme release. With increasing intracellular concentration of HPDs and with increasing intensity of the laser light, an increasing number of cells took up trypan blue and released the cytosolic enzyme lactate dehydrogenase and the lysosomal enzyme acid phosphatase after 1 h incubation of the irradiated cells at 37 degrees C. Cytotoxicity was less pronounced when the irradiated cells were incubated at 0 degree C indicating the involvement of enzyme reactions in cell death. No lipid peroxidation as measured by malondialdehyde and ethane formation was detectable. Our results suggest that during photodynamic therapy with HPDs and laser light epidermal keratinocytes may be seriously damaged. The data indicate that not lipid peroxidation but rather the activation of lysosomal enzymes is responsible for the cytotoxicity observed.
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
Artuc M,Ramshad M,Kappus Hdoi
10.1007/BF00510086subject
Has Abstractpub_date
1989-01-01 00:00:00pages
491-4issue
7eissn
0340-3696issn
1432-069Xjournal_volume
281pub_type
杂志文章abstract：:The effect of the proteolytic enzyme savinase on human plantar skin in vitro was examined by light and electron microscopy to elucidate the morphological involvement of activated savinase in human epidermis. Light microscopic examination of incubated skin fragments demonstrated that the histological changes produced b...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00371819
更新日期：1991-01-01 00:00:00
abstract：:A 2% commercial solution formulation of 5-fluorouracil (5-FU) was significantly better at delivering 5-FU than either a 1% or 5% solution. Among single component suspension formulations the highest transdermal delivery was obtained from the vehicle in which 5-FU was least soluble, isopropyl myristate (IPM), while chan...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00402624
更新日期：1990-01-01 00:00:00
abstract：:This study concerns 57 patients who fulfilled histological criteria for the diagnosis allergic vasculitis. For 37 of these patients, biopsy specimen were available from lesional and adjacent non-lesional skin. Histological signs of vasculitis were found at both sites, but in clinically normal skin the perivascular inf...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00406538
更新日期：1980-01-01 00:00:00
abstract：:Hydroperoxides of d-limonene were shown to be potent contact allergens when studied in guinea-pigs. Limonene-2-hydroperoxide (2-hydroperoxy-p-mentha-6,8-diene, a mixture of trans and cis isomers) was synthesized for the first time. The ratio between the trans and cis forms was 3:1. These two hydroperoxides were identi...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00370734
更新日期：1994-01-01 00:00:00
abstract：:Delayed pressure urticaria (DPU) is characterized by deep dermal wheals that appear in response to a local continuous pressure. Although it has been reported to complicate as many as 40% of cases of Caucasian patients with chronic urticaria, no definitive cases of Asian/Japanese patients have been reported in English ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-010-1053-4
更新日期：2010-10-01 00:00:00
abstract：:Polymorphonuclear leukocytes from patients with psoriasis demonstrated a significantly enhanced chemotactic responsiveness to zymosan-activated human serum as compared to granulocytes from healthy volunteers. Furthermore, psoriatic peripheral blood mononuclear cells stimulated with concanavalin a produced an increased...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00409454
更新日期：1981-01-01 00:00:00
abstract：:Dimethylfumarate (DMF), the essential ingredient of the drug product Fumaderm®, is used to treat psoriasis with a recognized favorable long-term safety profile. Interestingly, the mode of action and the pharmacokinetics of DMF in psoriasis or multiple sclerosis are not fully explored. It is known that DMF as an α,β-un...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-013-1332-y
更新日期：2013-07-01 00:00:00
abstract：:Solar radiation in the UVB range is absorbed primarily by the epidermal DNA where characteristic photodamage results in altered immune responses and mutagenic lesions. UVB exposure of the skin results in a profound upregulation of the anti-inflammatory cytokine, IL-10 and suppression of contact hypersensitivity (CHS)....
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-005-0634-0
更新日期：2006-03-01 00:00:00
abstract：:Patients with atopic dermatitis (AD) often present with dry skin, and the reduced secretion of sebum may be responsible for the impaired skin barrier function. A sebum check film enables the patient to self-evaluate the skin sebum content. This study compared the sebum check film with a sebumeter. The skin sebum conte...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-010-1076-x
更新日期：2010-11-01 00:00:00
abstract：:The injured skin produces a number of mediators that directly or indirectly modulate cell chemotaxis, migration, proliferation, and angiogenesis. Components of the kinin pathway including the kinin B1 receptor (B1R) have been found to occur in the human skin, but information about its role on keratinocyte biology is s...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-015-1595-6
更新日期：2015-11-01 00:00:00
abstract：:Classic Refsum disease (RD) is a rare, autosomal recessively-inherited disorder of peroxisome metabolism due to a defect in the initial step in the alpha oxidation of phytanic acid (PA), a C16 saturated fatty acid with four methyl side groups, which accumulates in plasma and lipid enriched tissues (please see van den ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-014-1478-2
更新日期：2014-10-01 00:00:00
abstract：:An ultrastructural investigation of two cutaneous lesions in a two-year-old Turkish boy with disseminated lipogranulomatosis (Farber) revealed curvilinear bodies in fibroblasts, histiocytes, and endothelial cells; "elongated membranes" in fibroblasts and endothelial cells; "zebra bodies" in endothelial cells; and spin...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00694628
更新日期：1979-10-01 00:00:00
abstract：:Vitiligo is an acquired clinical conundrum, characterized by white areas on the skin due to loss of functional melanocytes. Various studies demonstrated that vitiligo is associated with fewer risk of skin cancer but no exact molecular explanation for this interesting observation has been described. This article review...
journal_title：Archives of dermatological research
pub_type： 杂志文章,评审
doi：10.1007/s00403-011-1165-5
更新日期：2011-11-01 00:00:00
abstract：:Permeation enhancers are defined as substances that are capable of promoting penetration of drugs into skin and transdermal therapeutic systems offers a more reliable mean of administering drug through the skin. Skin is a natural barrier so it is necessary to employ enhancement strategies to improve topical bioavailab...
journal_title：Archives of dermatological research
pub_type： 杂志文章,评审
doi：10.1007/s00403-014-1445-y
更新日期：2014-07-01 00:00:00
abstract：:In 8 patients with idiopathic chronic urticaria suction blisters were produced simultaneously on lesional and perilesional skin in the periumbilical area without application of heat. The suction pressure was 250 mm Hg, and thus the blister production lasted from 60--120 min. The blister fluid histamine was determined ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00418572
更新日期：1979-11-01 00:00:00
abstract：:Role of neurotrophic factors including nerve growth factor (NGF) in the mechanism of overgrowth and hypersensitivity of sensory nerve in atopic dermatitis (AD) has been proposed. Glial cell line-derived neurotrophic factor (GDNF) is a member of neurotrophic factors of the nervous systems; however, the role of GDNF in ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-008-0856-z
更新日期：2008-08-01 00:00:00
abstract：:Mitochondrial DNA (mtDNA) mutations have recently been proposed as important contributors to ageing and degenerative diseases. In this study, we applied the polymerase chain reaction technique to detect 4.977 bp-deleted mtDNA, the most common and abundant deletion of mtDNA, in normal human skin. None of the skin speci...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00371798
更新日期：1994-01-01 00:00:00
abstract：:The incidence of cutaneous malignant melanoma continues to rise. Strategies to reduce mortality in this situation include improving therapy, encouraging earlier diagnosis, and promoting prevention. New developments such as epiluminescence, sentinel node biopsy, arterial limb perfusion and the use of vaccines are discu...
journal_title：Archives of dermatological research
pub_type： 杂志文章,评审
doi：10.1007/BF00370712
更新日期：1994-01-01 00:00:00
abstract：:In many skin diseases such as Demodex folliculitis, rosacea- or steroid-induced rosacea Demodex mites are present in abundance and are at least partially held responsible for causing these disorders. Although it is known that these diseases respond well to tetracyclines, it is unclear if this is due to the antiinflamm...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-009-0984-0
更新日期：2009-10-01 00:00:00
abstract：:In order to find, whether 8-MOP has an inhibiting effect on the proliferation of lymphocytes in the dark, we studied in vitro the lymphocyte proliferation by a mitogen (HgCl2) and by an antigen (tuberculin) from 16 psoriatic patients during the first oral PUVA treatment immediately before 8-MOP intake (03.--0.5 mg/kg ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00455295
更新日期：1978-05-31 00:00:00
abstract：:Six cases of dermatofibrosarcoma protuberans were studied ultrastructurally. Biopsy material from all six cases as well as cell cultures derived from four cases were examined. In all cases, the tumor cells in vivo and in vitro were related to fibroblasts. The two cases with histiocytoid features and a small spiral pat...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00409221
更新日期：1985-01-01 00:00:00
abstract：:Human alpha 1(I) and alpha 1(III) procollagen mRNA expression in skin tissue from 15 systemic sclerosis (SSc) patients and from 7 normal control subjects was quantitatively analyzed using in situ hybridization. The grains accumulating in each area, representing procollagen mRNA expression per cell, were counted. To no...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030050458
更新日期：1999-11-01 00:00:00
abstract：:The expression and location of the Ki-67 antigen was investigated in 62 basal cell carcinomas (BCC) using immunostaining techniques (PAP and APAAP) on cryostat sections. The tumor samples were classified into three groups according to microarchitecture (nodular, superficial or fibrosing). The Ki-67 growth fraction dis...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00371599
更新日期：1993-01-01 00:00:00
abstract：BACKGROUND:Anogenital warts are a common human papillomavirus infection. They cause emotional distress, especially when they are in the anogenital region. Cryotherapy is a first-line treatment. Previous clinical trials and case series have reported variable results with retinoids (isotretinoin) as adjuvant therapy. OB...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-020-02182-y
更新日期：2021-01-12 00:00:00
abstract：:Thymocyte selection-associated high-mobility group box (TOX) is aberrantly expressed in cutaneous T-cell lymphomas. In a recent study, TOX expression was noted unexpectedly in the follicle center (germinal center) B-cells of reactive lymph nodes and tonsils, used as external controls. To evaluate whether TOX is also e...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-016-1654-7
更新日期：2016-08-01 00:00:00
abstract：:Wound infections with multi-drug resistant bacteria increase morbidity and mortality and have considerable socioeconomic impact. They can lead to impaired wound healing, resulting in rising treatment costs. The aim of this study was to investigate an ex vivo human wound infection model. Human full-thickness skin from ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-009-1009-8
更新日期：2010-07-01 00:00:00
abstract：:Two male patients with longstanding contact sensitivity to chromium were treated with PUVA. One patient, suffering from concomitant photosensitivity, reacted very favorably; his skin lesions cleared and light tolerance increased. This was paralleled by a decrease in the photopatch test reactivity and by the extinction...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00403929
更新日期：1981-01-01 00:00:00
abstract：:Exposure to ultraviolet (UV) irradiation has detrimental effects on skin accompanied by the increased metabolism of hyaluronan (HA), a linear polysaccharide important for the normal physiological functions of skin. In this study, the modulation of human keratinocyte response to UVB irradiation by HA (970 kDa) was inve...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-011-1146-8
更新日期：2011-05-01 00:00:00
abstract：:Koebner response was studied in 35 psoriatic patients. Two punch biopsies per patient were taken from non-lesional psoriatic skin before, and 6 h, 2 days, 7 days, 14 days and 21 days after, tape stripping. Alterations in the numbers of CD1+ Langerhans cells, CD4+ and CD8+ T cells and CD11c+ macrophages were mapped mor...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00372065
更新日期：1992-01-01 00:00:00
abstract：:Recently, medium-dose UVA1 phototherapy (50 J/cm2) has been introduced as an effective treatment for severe atopic dermatitis (AD). In order to further elucidate the mechanisms by which medium-dose UVA1 irradiation leads to an improvement in skin status in patients with AD, biopsy specimens from ten patients before an...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030100216
更新日期：2001-04-01 00:00:00
