Abstract:
:The effect of the proteolytic enzyme savinase on human plantar skin in vitro was examined by light and electron microscopy to elucidate the morphological involvement of activated savinase in human epidermis. Light microscopic examination of incubated skin fragments demonstrated that the histological changes produced by savinase in the epidermis, including the stratum corneum, depended upon pH value, enzyme concentration and incubation time. After incubation in 0.1% savinase in phosphate buffer, pH 8.0, for 2 h at 35 degrees C, the stratum corneum revealed a serrated appearance at the free edges, the viable cell layers showed acantholysis, particularly in the suprabasal area. Digestion of cytoplasmic components of horny cells was observed by electron microscopy, but both cell membranes and desmosomes remained intact. Detachment of basal and spinous cells was found in the viable epidermal cell layers. The main cytoplasmic changes consisted of the presence of numerous cytoplasmic processes and vesicular formations. The results of this study indicate that activated savinase digests the intercellular substance in the viable cell layers, but it does not digest horny cells without physical rupture of the cell membrane.
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
Imai Sdoi
10.1007/BF00371819subject
Has Abstractpub_date
1991-01-01 00:00:00pages
377-81issue
6eissn
0340-3696issn
1432-069Xjournal_volume
283pub_type
杂志文章abstract::Adult female Wistar rats (n = 48) divided into four groups of 12 were treated orally with 3 mg/kg per day thalidomide, a 0.05% hexachlorobenzene (HCB)--containing diet, with both drugs together and with the vehicles (controls) over periods of 10 and 60 days. The protein and P450 contents and the activities of aminopyr...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00402227
更新日期:1994-01-01 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-009-0974-2
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030050359
更新日期:1998-11-01 00:00:00
abstract::Immigration of neutrophils into the epidermis is a hallmark of several so-called neutrophilic dermatoses. In this study we used a frozen section adhesion assay to study the impact of different activation procedures as well as of CD11 integrins on cell-cell adhesion between neutrophils and epidermal cells. Serial secti...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030050263
更新日期:1997-11-01 00:00:00
abstract::Infantile hemangioma (IH), the most common tumor of infancy, is characterized by rapid growth during infancy, followed by spontaneous involution over 5-10 years. Certain clinical observations have led to the suggestion that IH is triggered and maintained by hypoxia. We review the literature on the possible role of hyp...
journal_title:Archives of dermatological research
pub_type: 杂志文章,评审
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更新日期:2016-05-01 00:00:00
abstract::Close contacts between mast cells (MC) and nerve fibers have previously been demonstrated in normal and inflamed skin by light and electron microscopy. A key step for any study in MC-nerve interactions in situ is to simultaneously visualize both communication partners, preferably with the option of double labelling th...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030050195
更新日期:1997-04-01 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
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更新日期:2012-03-01 00:00:00
abstract::The treatment of epidermolysis bullosa acquisita (EBA) is difficult and often disappointing. We report on the successful treatment of two EBA patients with colchicine. The drug was administered orally at an initial dose of 2 mg/day. After 2 weeks of therapy a dramatic improvement was observed. Most of the cutaneous an...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00375841
更新日期:1994-01-01 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-015-1579-6
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-010-1076-x
更新日期:2010-11-01 00:00:00
abstract::Genetic abnormalities for different subtypes of epidermolysis bullosa (EB) have been described. In dominant simplex type EB, mutations of the K5 or K14 gene lead to disruption of basal cells and the formation of bullae. The recessive simplex types include EB with muscular dystrophy due to abnormal plectin, EB without ...
journal_title:Archives of dermatological research
pub_type: 杂志文章,评审
doi:10.1007/s00403-002-0369-0
更新日期:2003-04-01 00:00:00
abstract::Scleroderma is a heterogeneous disease with a complex etiology. As more information is gained about the underlying mechanisms and the improved classifications of scleroderma subtypes, treatments can be better personalized. Improving scleroderma patients' early diagnosis before end organ manifestations occur should imp...
journal_title:Archives of dermatological research
pub_type: 杂志文章,评审
doi:10.1007/s00403-016-1647-6
更新日期:2016-09-01 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-016-1685-0
更新日期:2016-11-01 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-005-0623-3
更新日期:2006-02-01 00:00:00
abstract::Test results are reported of the effect of salicylic acid on the vasoconstrictive effect of flumethasone pivalate. In specific, the depigmentation of the skin (identical with increase in lightness of the skin) following topical application of flumethasone pivalate was investigated with the aid of an improved spectroph...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00417384
更新日期:1981-01-01 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-009-1009-8
更新日期:2010-07-01 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00412738
更新日期:1990-01-01 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00417207
更新日期:1983-01-01 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00403801
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journal_title:Archives of dermatological research
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doi:10.1007/s00403-006-0664-2
更新日期:2006-06-01 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-002-0342-y
更新日期:2002-12-01 00:00:00
abstract::The distribution of several markers of keratinocyte differentiation was studied in normal epidermis, basal cell carcinomas (BCCs), and squamous cell carcinomas (SCCs) using the immunoperoxidase technique on frozen sections of punch biopsy specimens. As markers a panel of chain-specific monoclonal antibodies (MoAbs) di...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00426583
更新日期:1989-01-01 00:00:00
abstract::A recently described endogenous proteolytic process in pieces of human plantar stratum corneum incubated in vitro has been further studied. This process leads to a decrease in cohesion between the cells that had been facing outwards in vivo. Using two methods, that differed with respect to efficiency, to detach surfac...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00371642
更新日期:1990-01-01 00:00:00
abstract::Melanoma in situ (MIS) is a form of radial growth phase melanoma in which the proliferation of malignant cells is confined to the epidermis. Histologic features are invaluable in recognition of MIS. Regression occurs when the host's immune system attacks the primary melanocytic tumor cells via tumor infiltrate lymphoc...
journal_title:Archives of dermatological research
pub_type: 杂志文章,评审
doi:10.1007/s00403-020-02106-w
更新日期:2020-07-06 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-008-0884-8
更新日期:2008-10-01 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-009-1021-z
更新日期:2010-08-01 00:00:00
abstract::Tropospheric ozone (O3) is a source of oxidative stress. This study examined the ability of a topical antioxidant (WEL-DS) to inhibit O3-mediated damage in a human epidermal skin model. Four groups of tissues (N = 24) were compared: Group 1 (control) were untreated and unexposed; Group 2 were untreated and exposed to ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-020-02083-0
更新日期:2020-05-08 00:00:00
abstract::Tumor-associated antigens were demonstrated in phenol water extracts of human malignant melanoma by migration tests with leukocytes from melanoma patients and controls: in 25 out of 60 melanoma patients weak reactivity was observed, usually stimulation of leukocyte migration, while leukocytes from 37 controls were neg...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00417277
更新日期:1979-02-23 00:00:00
abstract::We have examined the relationship between topical psoriasis treatment and the activity of the mixed function oxidase, aryl hydrocarbon hydroxylase (AHH), in peripheral blood monocytes and lymphocytes from 20 patients with psoriasis. These data were compared to monocyte and lymphocyte AHH activity measured in 20 normal...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00511066
更新日期:1984-01-01 00:00:00