当前位置： SCI文献检索 > DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY期刊下所有文献 > β-Thymosins participate in antiviral immunity of red swamp crayfish (Procambarus clarkii).

β-Thymosins participate in antiviral immunity of red swamp crayfish (Procambarus clarkii).

Abstract：

:β-Thymosins participate in numerous biological activities, including cell proliferation and differentiation, wound healing, and anti-inflammatory and antimicrobial activities. Many studies have investigated vertebrate β-thymosins, whereas few reports have focused on invertebrate β-thymosins. In this study, nine isoforms of β-thymosins (PcThy-1 to PcThy-8) were identified from the red swamp crayfish Procambarus clarkii. The isoforms contained different numbers of the thymosin β actin-binding motif. PcThy-1 contained one thymosin β actin-binding motif, whereas PcThy-8 contained eight motifs. Western blot analysis with anti-PcThy-4 antibody showed that three to six isoforms were present in one tissue, and PcThy-4, PcThy-5, PcThy-6, and PcThy-7 were the main isoforms in several tissues. Time course expression analysis of PcThys at the protein level showed that PcThy-4 was upregulated in hemocytes and gills after white spot syndrome virus (WSSV) challenge. PcThy-4, which contained four thymosin β actin-binding motifs, was selected for further research. Tissue distribution analysis by quantitative real-time PCR showed that PcThy-4 was present in tissues of the hemocytes, heart, hepatopancreas, gills, stomach, and intestine at the transcriptional level. Transcriptional expression profiles showed that PcThy-4 was upregulated after WSSV challenge. In vivo RNAi and protein injection assay results showed that PcThy-4 inhibited the replication of WSSV in crayfish and enhanced the survival rate after WSSV infection. Furthermore, PcThy-4 promoted hemocyte phagocytosis of WSSV. Overall, results suggested that PcThys protected crayfish from WSSV infection and played an important role in antiviral immune response.

journal_name

Dev Comp Immunol

journal_title

Developmental and comparative immunology

authors

Shi XZ,Shi LJ,Zhao YR,Zhao XF,Wang JX

doi

10.1016/j.dci.2015.04.003

subject

Has Abstract

pub_date

2015-08-01 00:00:00

pages

213-25

issue

2

eissn

0145-305X

issn

1879-0089

pii

S0145-305X(15)00071-3

journal_volume

51

pub_type

杂志文章

相关文献

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY文献大全
  • Antisense approaches for elucidating ranavirus gene function in an infected fish cell line.

    abstract：:Viral virulence/immune evasion strategies and host anti-viral responses represent different sides of the continuing struggle between virus and host survival. To identify virus-encoding molecules whose function is to subvert or blunt host immune responses, we have adapted anti-sense approaches to knock down the express...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2010.12.002

    authors： Whitley DS,Sample RC,Sinning AR,Henegar J,Chinchar VG

    更新日期：2011-09-01 00:00:00

  • Two prophenoloxidases are important for the survival of Vibrio harveyi challenged shrimp Penaeus monodon.

    abstract：:Phenoloxidase (PO) plays an important role in arthropod melanization. Previously, a prophenoloxidase (PmproPO1) gene was cloned and characterized from the hemocytes of the black tiger shrimp, Penaeus monodon. In the present study, we report a novel proPO gene (PmproPO2) belonging to the proPO family identified from th...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2008.09.003

    authors： Amparyup P,Charoensapsri W,Tassanakajon A

    更新日期：2009-02-01 00:00:00

  • Functional elucidation of LvToll 3 receptor from P. vannamei through RNA interference and its potential role in the shrimp antiviral response.

    abstract：:There is a continuing debate on whether an antiviral immunity similar to vertebrate interferon response exists in invertebrates. Recent advances in penaeid immunology identified several new members of the Toll receptor family and one of these is LvToll3 (Litopenaeus vannamei Toll3). It is hypothesized in this study th...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2018.01.020

    authors： Guanzon DAV,Maningas MBB

    更新日期：2018-07-01 00:00:00

  • Regional and global changes in TCRalphabeta T cell repertoires in the gut are dependent upon the complexity of the enteric microflora.

    abstract：:The repertoire of gut associated T cells is shaped by exposure to microbes, including the natural enteric microflora. Previous studies compared the repertoire of gut associated T cell populations in germ free (GF) and conventional mammals often focussing on intra-epithelial lymphocyte compartments. Using GF, conventio...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2009.11.009

    authors： Mwangi WN,Beal RK,Powers C,Wu X,Humphrey T,Watson M,Bailey M,Friedman A,Smith AL

    更新日期：2010-04-01 00:00:00

  • A longitudinal study of the protein components of marsupial milk from birth to weaning in the tammar wallaby (Macropus eugenii).

    abstract：:The major milk whey proteins of the tammar wallaby (Macropus eugenii) have been identified over the total period of lactation using proteomic analysis techniques comprising two-dimensional electrophoresis, comparative image analysis, matrix assisted laser desorption ionisation mass spectrometry (MALDI MS), de novo pep...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2008.08.002

    authors： Joss JL,Molloy MP,Hinds L,Deane E

    更新日期：2009-02-01 00:00:00

  • Identification and molecular characterization of a complement C3 molecule in a lophotrochozoan, the Hawaiian bobtail squid Euprymna scolopes.

    abstract：:Examination of the EST database of the light organ of the Hawaiian bobtail squid Euprymna scolopes revealed a sequence with similarity to complement C3. RACE yielded the full open reading frame of this protein. Analysis of the resultant sequence revealed that Es-C3 (E. scolopes-C3) has conserved residues and domains k...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2008.07.013

    authors： Castillo MG,Goodson MS,McFall-Ngai M

    更新日期：2009-01-01 00:00:00

  • Phenoloxidase-associated cellular defence in the Sydney rock oyster, Saccostrea glomerata, provides resistance against QX disease infections.

    abstract：:The enzyme phenoloxidase is a critical component of the immunological defence of invertebrates. Previously, we have shown that the activity of phenoloxidase in Sydney rock oysters (Saccostrea glomerata) correlates with the severity of QX disease outbreaks. The aetiological agent of QX disease is the opportunistic prot...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2007.06.006

    authors： Butt D,Raftos D

    更新日期：2008-01-01 00:00:00

  • Double-stranded RNA and antiviral immunity in marine shrimp: inducible host mechanisms and evidence for the evolution of viral counter-responses.

    abstract：:Double-stranded RNA (dsRNA) is a common virus-associated molecular pattern and a potent inducer of antiviral responses in many organisms. While it is clear that the specific RNA interference (RNAi) response, a phenomenon triggered by dsRNA, serves antiviral functions in invertebrates, innate (non-specific) antiviral i...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.dci.2006.08.011

    authors： Robalino J,Bartlett TC,Chapman RW,Gross PS,Browdy CL,Warr GW

    更新日期：2007-01-01 00:00:00

  • The ontogeny of the porcine immune system.

    abstract：:Cellular and humoral aspects of the immune response develop sequentially in the fetus. During the ontogeny, the pluripotent stem cells emerge and differentiate into all hematopoietic lineages. Basic questions including the identification of the first lympho-hematopoietic sites, the origin of T and B lymphocytes, the d...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.dci.2008.07.011

    authors： Sinkora M,Butler JE

    更新日期：2009-03-01 00:00:00

  • The occurrence of three D-J-C clusters within the dromedary TRB locus highlights a shared evolution in Tylopoda, Ruminantia and Suina.

    abstract：:The αβ T cells are important components of the adaptive immune system and can recognize a vast array of peptides presented by MHC molecules. The ability of these T cells to recognize the complex depends on the diversity of the αβ TR, which is generated by a recombination of specific Variable, Diversity and Joining gen...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2017.05.021

    authors： Antonacci R,Bellini M,Pala A,Mineccia M,Hassanane MS,Ciccarese S,Massari S

    更新日期：2017-11-01 00:00:00

  • Serpin-15 from Bombyx mori inhibits prophenoloxidase activation and expression of antimicrobial peptides.

    abstract：:Serine protease inhibitors (SPIs) play a key role in physiological responses by controlling protease activities. In this study, we studied the biochemical functions of serpin-15, an SPI, from Bombyx mori (Bmserpin-15). Recombinant Bmserpin-15 was expressed in Escherichia coli cells and used to raise rabbit anti-Bmserp...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2015.02.013

    authors： Liu D,Wang L,Yang L,Qian C,Wei G,Dai L,Li J,Zhu B,Liu C

    更新日期：2015-07-01 00:00:00

  • Black carp RIPK1 negatively regulates MAVS-mediated antiviral signaling during the innate immune activation.

    abstract：:Receptor-interacting serine/threonine protein kinase 1 (RIPK1) is an important regulator of necroptosis and involved in innate immune response in human and mammal; however, its function in teleost fish mains largely unknown. In this paper, the RIPK1 homologue of black carp (Mylopharyngodon piceus) has been cloned and ...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2020.103726

    authors： Xie X,Cao Y,Dai Y,Chen Z,Wei J,Tan Y,Wu H,Feng H

    更新日期：2020-08-01 00:00:00

  • Ontogeny of the thymus in a teleost fish, Cyprinus carpio L.: developing thymocytes in the epithelial microenvironment.

    abstract：:A monoclonal antibody, WCL9, specific for membrane molecules of a thymocyte subpopulation was used to detect these cells in situ during the ontogeny of thymus. Cryo-sections revealed WCL9+ cells in the rudiment of the thymus (day 4 post fertilization); thereafter, the positive cells were observed exclusively in the co...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/s0145-305x(98)00053-6

    authors： Romano N,Taverne-Thiele AJ,Fanelli M,Baldassini MR,Abelli L,Mastrolia L,Van Muiswinkel WB,Rombout JH

    更新日期：1999-03-01 00:00:00

  • Five subfamilies of β-defensin genes are present in salmonids: Evolutionary insights and expression analysis in Atlantic salmon Salmo salar.

    abstract：:β-defensins (BD) are the largest family of vertebrate defensins with potent antimicrobial, chemotactic and immune-regulatory activities. Four BD genes (BD1-4) have been cloned previously in rainbow trout but none have been reported in other salmonids. In this study seven BD genes (BD1a-b, 2-4, 5a-b) are characterised ...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2019.103560

    authors： Harte A,Tian G,Xu Q,Secombes CJ,Wang T

    更新日期：2020-03-01 00:00:00

  • Lectins and lectin-like molecules in lower plants. II. Freshwater algae.

    abstract：:Freshwater chlamydomonad algae possess flagella surface isoagglutinins of a glycoprotein nature which are involved in gamete recognition and consequent cell fusion. Alternatively, such molecules may have a receptor role in certain Chlamydomonas species. In some freshwater volvocaines, lectin-like substances act as ind...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章,评审

    doi：10.1016/0145-305x(85)90002-3

    authors： Ingram GA

    更新日期：1985-07-01 00:00:00

  • Trypanosoma carassii hsp70 increases expression of inflammatory cytokines and chemokines in macrophages of the goldfish (Carassius auratus L.).

    abstract：:We report on the cloning and characterization of Trypanosoma carassii 70 KDa heat shock protein (hsp70). T. carassii hsp70 was secreted/excreted into culture medium in vitro and was recognized by sera from infected fish. Recombinant hsp70 (rhsp70) activated goldfish macrophages and stimulated the production of pro-inf...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2009.06.003

    authors： Oladiran A,Belosevic M

    更新日期：2009-10-01 00:00:00

  • Localization and bacteriostasis of Vibrio introduced into the Pacific white shrimp, Litopenaeus vannamei.

    abstract：:Although numerous mechanisms of immune defense have been described in crustaceans, the tissue distribution and fate of live bacteria introduced into the host remain unclear. In the present study, Litopenaeus vannamei were injected with a sub-lethal dose of kanamycin-resistant Vibrio campbellii expressing green fluores...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2005.01.004

    authors： Burgents JE,Burnett LE,Stabb EV,Burnett KG

    更新日期：2005-01-01 00:00:00

  • Genetic and molecular basis of the immune system in the brachiopod Lingula anatina.

    abstract：:The extension of comparative immunology to non-model systems, such as mollusks and annelids, has revealed an unexpected diversity in the complement of immune receptors and effectors among evolutionary lineages. However, several lophotrochozoan phyla remain unexplored mainly due to the lack of genomic resources. The in...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2017.12.021

    authors： Gerdol M,Luo YJ,Satoh N,Pallavicini A

    更新日期：2018-05-01 00:00:00

  • Purification and properties of agglutinins from conger eel, Conger Myriaster (Brevoort), skin mucus.

    abstract：:The skin mucus of the conger eel, Conger myriaster, contains galactose-specific agglutinins. Hemagglutinating activity is independent of divalent cations and is destroyed by heating at 50 degrees C for 15 min. The mucus agglutinins, named congerins, are a mixture of proteins with different electrical charges. Three of...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/0145-305x(88)90007-9

    authors： Kamiya H,Muramoto K,Goto R

    更新日期：1988-04-01 00:00:00

  • Effects of Trichobilharzia ocellata on hemocytes of Lymnaea stagnalis.

    abstract：:We analyzed the effects of infection with Trichobilharzia ocellata on hemocytes of its snail host, Lymnaea stagnalis, and correlated them with successive stages of parasite development. Circulating hemocytes were studied at 0, 2, 4, 6, and 8 weeks post exposure (p.e.) with respect to cell number, distribution of subpo...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/0145-305x(91)90001-f

    authors： Amen RI,Tijnagel JM,van der Knaap WP,Meuleman EA,de Lange-de Klerk ES,Sminia T

    更新日期：1991-04-01 00:00:00

  • Identification of a novel relish homolog in Chinese shrimp Fenneropenaeus chinensis and its function in regulating the transcription of antimicrobial peptides.

    abstract：:Penaeid shrimp, as an invertebrate, relies on the innate immunity to oppose the microbial invaders. Antimicrobial peptides (AMP) are an integral component of the innate immune system in most organisms and function as an early first line of defense against pathogens, but the knowledge about the pathways to regulate the...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2009.06.001

    authors： Li F,Yan H,Wang D,Priya TA,Li S,Wang B,Zhang J,Xiang J

    更新日期：2009-10-01 00:00:00

  • Sex-associated differences in the mitogenic responsiveness of snake blood lymphocytes.

    abstract：:The blastogenic response of male and female snake (Psammophis sibilans) peripheral blood lymphocytes to concanavalin A, phytohaemagglutinin, and E. coli lipopolysaccharide was tested for putative sex-associated differences. Data obtained suggest that normal female blood lymphocytes respond relatively vigorous in compa...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/0145-305x(89)90003-7

    authors： Saad AH

    更新日期：1989-07-01 00:00:00

  • In situ lymphocyte apoptosis in larval Xenopus laevis, the South African clawed toad.

    abstract：:During Anuran metamorphosis larval structures regress, adult structures form anew and impaired T cell immune functions are noted, as are alterations in endogenous glucocorticoid titers. In situ histological data, after staining for DNA fragmentation, reveal patterns of lymphocyte suicide in the thymus and spleen of no...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/s0145-305x(97)00057-8

    authors： Grant P,Clothier RH,Johnson RO,Ruben LN

    更新日期：1998-07-01 00:00:00

  • Metabolic responses of clam Ruditapes philippinarum exposed to its pathogen Vibrio tapetis in relation to diet.

    abstract：:We investigated the effect of brown ring disease (BRD) development and algal diet on energy reserves and activity of enzymes related to energy metabolism, antioxidant system and immunity in Manila clam, Ruditapes philippinarum. We found that algal diet did not impact the metabolic response of clams exposed to Vibrio t...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2016.02.026

    authors： Richard G,Guérard F,Corporeau C,Lambert C,Paillard C,Pernet F

    更新日期：2016-07-01 00:00:00

  • The genomic structure, alternative splicing and immune response of Chlamys farreri thioester-containing protein.

    abstract：:CfTEP is a member of thioester-containing protein (TEP) family found in Zhikong scallop Chlamys farreri and is involved in innate immunity against invading microbes. In the present study, the genomic DNA of CfTEP was cloned and characterized. The genomic DNA sequence of CfTEP consisted of 40 exons and 39 introns spann...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2009.05.007

    authors： Zhang H,Wang L,Song L,Zhao J,Qiu L,Gao Y,Song X,Li L,Zhang Y,Zhang L

    更新日期：2009-10-01 00:00:00

  • Panusin represents a new family of β-defensin-like peptides in invertebrates.

    abstract：:Beta_defensin have been solely found in vertebrates until β-defensin-like peptides were described as transcript isoforms in two species of Panulirus genus. They were considered as putative antimicrobials since their biological activity have not been demonstrated. Here we purified and characterized a defensin-like pept...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2016.09.002

    authors： Montero-Alejo V,Corzo G,Porro-Suardíaz J,Pardo-Ruiz Z,Perera E,Rodríguez-Viera L,Sánchez-Díaz G,Hernández-Rodríguez EW,Álvarez C,Peigneur S,Tytgat J,Perdomo-Morales R

    更新日期：2017-02-01 00:00:00

  • Avian IgY is selectively incorporated into the egg yolks of oocytes by discriminating Fc amino acid residues located on the Cυ3/Cυ4 interface.

    abstract：:In avian species, maternal IgY is selectively incorporated into the egg yolks of maturing oocytes, but the relevance of receptor-mediated uptake is unclear. Here we investigated the critical amino acid residues of IgY required for egg yolk transport by conducting mutational analyses of selected residues located along ...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2012.12.003

    authors： Murai A,Murota R,Doi K,Yoshida T,Aoyama H,Kobayashi M,Horio F

    更新日期：2013-04-01 00:00:00

  • Transfection of crayfish hematopoietic tissue cells.

    abstract：:Transfection is a powerful tool useful for studying gene function. Establishing transfection methods that enable highly efficient DNA uptake has become increasingly important. The crayfish hematopoietic tissue (Hpt) cell cultures have been proven to be suitable for studies on immunity and cell differentiation in crust...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2018.07.005

    authors： Shi H,Ruan L,Söderhäll I,Söderhäll K,Xu X

    更新日期：2018-11-01 00:00:00

  • WITHDRAWN: Development of an RT-PCR-RFLP assay for the detection and differentiation of wild-type and vaccine strains of classical swine fever virus.

    abstract：:This article has been withdrawn at the request of the author(s). The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2012.05.002

    authors： Hu J,Shan H,Wang S,Yang P,Wang Y,Sun H,Huang J,Yang R,Zhang C,Qin X

    更新日期：2012-05-15 00:00:00

  • Molecular cloning of the porcine RANTES promoter: functional characterization of dsDNA/dsRNA response elements in PK-15 cells.

    abstract：:The chemokine RANTES plays an essential role in inflammation and immune response. In this study, we cloned the nucleotide sequence of the 5'-flanking region of the porcine RANTES (poRANTES) gene and characterized the regulatory elements that activate transcription. Analyses of a series of 5' deletion constructs demons...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2010.10.008

    authors： Wang D,Fang L,Li P,Chen Q,Luo R,Chen H,Xiao S

    更新日期：2011-03-01 00:00:00