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Antisense approaches for elucidating ranavirus gene function in an infected fish cell line.

Abstract：

:Viral virulence/immune evasion strategies and host anti-viral responses represent different sides of the continuing struggle between virus and host survival. To identify virus-encoding molecules whose function is to subvert or blunt host immune responses, we have adapted anti-sense approaches to knock down the expression of specific viral gene products. Our intention is to correlate knock down with loss of function and thus infer the role of a given viral gene. As a starting point in this process we have targeted several structural and catalytic genes using antisense morpholino oligonucleotides (asMO) and small, interfering RNAs (siRNA). In proof of concept experiments we show the feasibility of this approach and describe recent work targeting five frog virus 3 genes. Our results indicate that both 46K and 32R, two immediate-early viral proteins, are essential for replication in vitro, and confirm earlier findings that the major capsid protein, the largest subunit of the viral homolog of RNA polymerase II, and the viral DNA methyltransferase are also essential for replication in cell culture.

journal_name

Dev Comp Immunol

journal_title

Developmental and comparative immunology

authors

Whitley DS,Sample RC,Sinning AR,Henegar J,Chinchar VG

doi

10.1016/j.dci.2010.12.002

subject

Has Abstract

pub_date

2011-09-01 00:00:00

pages

937-48

issue

9

eissn

0145-305X

issn

1879-0089

pii

S0145-305X(10)00286-7

journal_volume

35

pub_type

杂志文章

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