Abstract：
:The function and mechanism of chitinases in early embryonic development remain largely unknown. We show here that recombinant chitinase-3 (rChi3) is able to hydrolyze the artificial chitin substrate, 4-methylumbelliferyl-β-D-N,N',N″-triacetylchitotrioside, and to bind to and inhibit the growth of the fungus Candida albicans, implicating that Chi3 plays a dual function in innate immunity and chitin-bearing food digestion in zebrafish. This is further corroborated by the expression profile of Chi3 in the liver and gut, which are both immune- and digestion-relevant organs. Compared with rChi3, rChi3-CD lacking CBD still retains partial capacity to bind to C. albicans, but its enzymatic and antifungal activities are significantly reduced. By contrast, rChi3-E140N with the putative catalytic residue E140 mutated shows little affinity to chitin, and its enzymatic and antifungal activities are nearly completely lost. These suggest that both enzymatic and antifungal activities of Chi3 are dependent on the presence of CBD and E140. We also clearly demonstrate that in zebrafish, both the embryo extract and the developing embryo display antifungal activity against C. albicans, and all the findings point to chitinase-3 (Chi3) being a newly-identified factor involved in the antifungal activity. Taken together, a dual function in both innate immunity and food digestion in embryo is proposed for zebrafish Chi3. It also provides a new angle to understand the immune role of chitinases in early embryonic development of animals.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Teng Z,Sun C,Liu S,Wang H,Zhang Sdoi
10.1016/j.dci.2014.06.008subject
Has Abstractpub_date
2014-10-01 00:00:00pages
489-98issue
2eissn
0145-305Xissn
1879-0089pii
S0145-305X(14)00164-5journal_volume
46pub_type
杂志文章abstract：:The immune system plays an important role in pregnancy. Chemokines recruit leukocytes at the maternal-fetal interface during early pregnancy. However, the role of the chemokine, C-C motif chemokine ligand 21 (CCL21), is less known. The aim of this study was to identify the expression of CCL21 and its receptor, CCR7, i...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2019.01.016
更新日期：2019-05-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/s0145-305x(00)00022-7
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abstract：:Yellow head virus (YHV) is one of the most widespread viruses seriously affecting black tiger shrimp (Penaeus monodon) cultivation. A previous microarray study demonstrated that clathrin coat assembly protein 17 (AP17) was significantly up-regulated after YHV infection (Pongsomboon et al., 2011). Clathrin coat AP17 is...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2013.11.017
更新日期：2014-05-01 00:00:00
abstract：:Ants, as eusocial insects, live in dense groups with high connectivity, increasing the risk of pathogen spread and possibly driving the evolution of their antimicrobial immune system. Draft genomes of seven ant species provide a new source to undertake comparative study of their antimicrobial peptides (AMPs), key comp...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2012.05.003
更新日期：2012-10-01 00:00:00
abstract：:Much is now known about the vertebrate mechanisms involved in mucosal immunity, and the requirement of commensal microbiota at mucosal surfaces for the proper functioning of the immune system. In comparison, very little is known about the mechanisms of immunity at the barrier epithelia of non-vertebrate organisms. The...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章,评审
doi：10.1016/j.dci.2013.03.009
更新日期：2013-07-01 00:00:00
abstract：:During Anuran metamorphosis larval structures regress, adult structures form anew and impaired T cell immune functions are noted, as are alterations in endogenous glucocorticoid titers. In situ histological data, after staining for DNA fragmentation, reveal patterns of lymphocyte suicide in the thymus and spleen of no...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/s0145-305x(97)00057-8
更新日期：1998-07-01 00:00:00
abstract：:Serine protease inhibitors (SPIs) play a key role in physiological responses by controlling protease activities. In this study, we studied the biochemical functions of serpin-15, an SPI, from Bombyx mori (Bmserpin-15). Recombinant Bmserpin-15 was expressed in Escherichia coli cells and used to raise rabbit anti-Bmserp...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2015.02.013
更新日期：2015-07-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2014.03.001
更新日期：2014-07-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2012.11.003
更新日期：2013-03-01 00:00:00
abstract：:The repertoire of gut associated T cells is shaped by exposure to microbes, including the natural enteric microflora. Previous studies compared the repertoire of gut associated T cell populations in germ free (GF) and conventional mammals often focussing on intra-epithelial lymphocyte compartments. Using GF, conventio...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2009.11.009
更新日期：2010-04-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2016.09.014
更新日期：2017-02-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2008.11.011
更新日期：2009-05-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2019.01.009
更新日期：2019-05-01 00:00:00
abstract：:Though Bombyx mori larvae possess five types of hemocytes in the hemolymph, the granular cells play a key role as phagocytes and also participate in the early phase of encapsulation together with plasmacytes. In this sense, these two hemocytes are regarded as immunocompetent cells in B.mori. A granular cell can intern...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章,评审
doi：
更新日期：1982-10-01 00:00:00
abstract：:A new lily-type lectin RbLTL was identified from rock bream (Oplegnathus fasciatus) and its expression analysed. In this study, a new lily-type lectin gene (RbLTL) was cloned from rock bream using expressed sequence tag (EST) analysis. The full-length RbLTL cDNA was encoding a 117-amino acid protein. The deduced amino...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2016.06.014
更新日期：2016-12-01 00:00:00
abstract：:Differences in responses of chicken bone marrow derived dendritic cells (BMDC) to in vitro treatment with lipopolysaccharide (LPS), heat, and LPS + heat were identified. The Fayoumi is more disease resistant and heat tolerant than the Leghorn line. Nitric Oxide (NO) production, phagocytic ability, MHC II surface expre...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2016.05.016
更新日期：2016-10-01 00:00:00
abstract：:This study was aimed at the identification of variations in humoral immunity parameters during the lactation period in macaque females, a recognized model in reproductive physiology and pathology. The importance of such evaluation is evidenced in particular by the central role played by the maternally transferred immu...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(96)00006-7
更新日期：1996-03-01 00:00:00
abstract：:Dairy cows undergo a nutrient deficit immediately postpartum when lactational demands exceed nutrient intake. This occurs concurrently to an increased challenge due to bacterial and viral infections, yet ability for pathogen clearance is reduced despite a heightened and often host-damaging inflammatory response. We hy...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2018.11.021
更新日期：2019-04-01 00:00:00
abstract：:Infection of turkeys with the haemorrhagic enteritis virus (HEV), a type II avian adenovirus, results in varying rates of morbidity and mortality. The disease is characterised by splenomegaly, intestinal haemorrhage, sudden death and immunosuppression. The mechanisms of HEV immunopathogenesis and immunosuppression are...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章,评审
doi：10.1016/s0145-305x(99)00075-0
更新日期：2000-03-01 00:00:00
abstract：:Studies were undertaken to determine whether previously described reductions in splenic DC-SIGN expression in simian acquired immune deficiency syndrome (AIDS) are limited to pathogenic simian immunodeficiency virus (SIV) infection. DC-SIGN expression was evaluated by immunohistochemistry in lymphoid tissues from AIDS...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2008.06.006
更新日期：2008-01-01 00:00:00
abstract：:Infectious hematopoietic necrosis virus (IHNV) is a common pathogen that causes severe disease in the salmonid aquaculture industry. Recent work demonstrated that autophagy plays an important role in pathogen invasion by activating innate and adaptive immunity. This study investigated the relationship between IHNV and...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2017.07.025
更新日期：2017-12-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2006.07.009
更新日期：2007-01-01 00:00:00
abstract：:The development of mammalian megakaryocytes and platelets is regulated by numerous cytokine signals, primarily through the thrombopoietin (TPO)/c-MPL axis. Although non-mammalian vertebrates are known to possess nucleated thrombocytes functionally equivalent to mammalian platelets, the dynamics of the thrombocyte deve...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2018.03.005
更新日期：2018-07-01 00:00:00
abstract：:The Duck interferon gamma (DuIFN-gamma) cDNA was cloned from a phytohaemaglutinin-stimulated duck spleen cDNA library screened using a chicken IFN-gamma (ChIFN-gamma) cDNA probe. The DuIFN-gamma cDNA is 1392 nt long and shows 99% and 80% sequence identity with another cloned DuIFN-gamma cDNA, and with ChIFN-gamma cDNA...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/s0145-305x(00)00041-0
更新日期：2001-01-01 00:00:00
abstract：:The in vitro mitogenic responses of channel catfish peripheral blood leucocytes to ConA and LPS were differentially affected by both in vitro and in vivo temperatures. The magnitude of the response to LPS was relatively independent of both in vitro culture temperature and in vivo acclimation temperature. The magnitude...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(84)90038-7
更新日期：1984-04-01 00:00:00
abstract：:Up to now for Swine Workshop Cluster 2 (SWC2) the orthologous human CD molecule was unknown. By use of the SWC2-specific mAb b30c7 and a retroviral cDNA expression library derived from stimulated porcine peripheral blood mononuclear cells we could identify SWC2 as porcine CD27. Phenotypic analyses of lymphocytes isola...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2012.06.011
更新日期：2012-10-01 00:00:00
abstract：:Infectious bursal disease (IBD) is an economically important immunosuppressive disease of chickens. The IBD virus (IBDV) actively replicates in B cells and causes severe bursal damage. Generally, T cells are refractory to infection with IBDV but are known to promote virus clearance. However, the mechanisms of T cell m...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2011.01.007
更新日期：2011-05-01 00:00:00
abstract：:Toll-like Receptors (TLRs) are the first innate receptors in recognizing pathogen-associated molecular patterns. In fish, upregulation of toll-like receptors during infection has been largely demonstrated while the effects of abiotic stressors on their expression remain poorly investigated. In this study, striped catf...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2017.03.020
更新日期：2017-08-01 00:00:00
abstract：:Ascorbate is an essential nutrient commonly regarded as an antioxidant. In this study, using axenic brine shrimp and pathogenic strain Vibrio harveyi as the host-pathogen model, we confirmed that pretreatment of sodium ascorbate (NaAs), at an optimum concentration, was a prooxidant by generation of hydrogen peroxide, ...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2018.11.007
更新日期：2019-03-01 00:00:00
abstract：:Duck (Anas platyrhynchos) blood lymphocytes were cultured in the presence of phytohaemagglutinin (PHA), concanavalin A (Con A), pokeweed mitogen (PWM), lentil lectin (LL), Roman snail lectin (HP), wheat germ agglutinin (WGA), Bandeiraea simplicifolia seed lectin (BSS), peanut agglutinin (PNA), horseshoe crab lectin (H...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(91)90028-w
更新日期：1991-10-01 00:00:00
