Abstract:
:Ants, as eusocial insects, live in dense groups with high connectivity, increasing the risk of pathogen spread and possibly driving the evolution of their antimicrobial immune system. Draft genomes of seven ant species provide a new source to undertake comparative study of their antimicrobial peptides (AMPs), key components of insect innate immunity. By using computational approaches, we analyzed five AMP families that include abaecins, hymenoptaecins, insect defensins, tachystatins, and crustins in ants, which comprise 69 new members. Among them, a new type of proline-rich abaecins was recognized and they are exclusively present in ants. Hymenoptaecins, a family of glycine-rich AMPs from Hymenoptera and Diptera, exhibit variable numbers of intragenic tandem repeats in a lineage-specific manner and all hymenoptaecins in ants have evolved an acidic C-terminal propeptide. In some ant species, insect defensins with the cysteine-stabilized α-helical and β-sheet (CSαβ) fold and tachystatin-like AMPs with the inhibitor cysteine knot (ICK) fold have undergone gene expansion and differential gene loss. Moreover, extensive sequence diversity exists in the C-termini of the defensins and the ICK-type peptides and the n-loop of the defensins. Also, we identified for the first time a crustin-type AMP in ants, which are only known in crustaceans previously. These ant crustins evolutionarily gain an aromatic amino acid-rich insertion when compared with those of crustaceans. Our work not only enlarges the insect AMP resource, but also sheds light on the complexity and dynamic evolution of AMPs in ants.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Zhang Z,Zhu Sdoi
10.1016/j.dci.2012.05.003subject
Has Abstractpub_date
2012-10-01 00:00:00pages
262-74issue
2eissn
0145-305Xissn
1879-0089pii
S0145-305X(12)00090-0journal_volume
38pub_type
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