Abstract:
:The presence of phenoloxidase (PO) activity was detected in different developmental stages of the Pacific oyster, Crassostrea gigas. A significant reduction in PO activity was observed from the 6h embryo stage to the day 11 larvae by spectrophotometry. A progressive increase was also observed from the day 13 larvae right through to the juvenile stage. The microscopy studies with '6h embryo' and adult samples confirmed the presence of PO activity. Various modulators of PO activity were used to study the triggering of pro-phenoloxidase (proPO) activating system of C. gigas but also to confirm the exact nature of the monitored activity. The enzyme activation mechanisms appear to differ with the developmental stage: bacterial lipopolysaccharides constitute an early elicitor of the proPO-PO system, whereas a purified trypsin triggers proPO-PO system in C. gigas spat. Phenoloxidase activity was totally suppressed by PO-specific inhibitors such as beta-2-mercaptoethanol, sodium diethyldithiocarbonate and tropolone. This study demonstrated the selective response of PO-like activity by different elicitors and suggested that proPO-PO activating system, which is supposed to play an important function in non-self recognition and host immune reactions in oyster, is expressed early in the Pacific oyster, C. gigas.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Thomas-Guyon H,Gagnaire B,Bado-Nilles A,Bouilly K,Lapègue S,Renault Tdoi
10.1016/j.dci.2008.11.011subject
Has Abstractpub_date
2009-05-01 00:00:00pages
653-9issue
5eissn
0145-305Xissn
1879-0089pii
S0145-305X(08)00255-3journal_volume
33pub_type
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