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Expression of an inducible nitric oxide synthase gene in rainbow trout Oncorhynchus mykiss.

Abstract：

:Using oligonucleotide primers based on mammalian nitric oxide synthases (NOS), expression of an inducible NOS (iNOS) gene was detected in head kidney and gill tissue of bacterially-challenged rainbow trout. Three overlapping fragments were amplified by RT-PCR and used to construct a contiguous sequence of 1410bp, with high nucleotide homology to iNOS in birds (61%) and mammals (57-59%). The nucleotide sequence translated in one reading frame to produce a partial peptide containing 470 amino acids, with 69-71% amino acid homology with mammalian iNOS, 81% homology with chicken iNOS and 85% homology with a partial (492bp) goldfish iNOS sequence. In vitro stimulation of head kidney macrophages with LPS also induced expression of the trout iNOS RNA, with optimal expression seen using 20-50 microg/ml LPS at 2h to 6h post-stimulation. The evolutionary and functional significance of the trout iNOS sequence are discussed.

journal_name

Dev Comp Immunol

journal_title

Developmental and comparative immunology

authors

Laing KJ,Hardie LJ,Aartsen W,Grabowski PS,Secombes CJ

doi

10.1016/s0145-305x(98)00036-6

keywords:

subject

Has Abstract

pub_date

1999-01-01 00:00:00

pages

71-85

issue

1

eissn

0145-305X

issn

1879-0089

pii

S0145-305X(98)00036-6

journal_volume

23

pub_type

杂志文章

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