Abstract：
:The full length coding sequence of the cattle transcription factor p65 was isolated and cloned. The cloned bovine p65 was expressed in mammalian cells, and it induced the NF-kappaB-specific luciferase reporter gene expression. Using gel retardation techniques, we demonstrated that the cloned bovine p65 bound to the consensus kappaB sequence. The comparison of the bovine p65 with its human and mouse orthologues at amino acid level showed high homology in both the DNA-binding domain, known as Rel homology domain (RHD) and the transactivation domain (TAD). The phylogenetic analysis at DNA level provided a new insight in the evolution of the NF-kappaB family, and it could resolve the topology of the mammalian p65molecules. Although, the RHD was conserved in vertebrates, the TAD sequences deviated from each other, and showed faster molecular evolution than RHD sequences, which may indirectly result in the modification of NF-kappaB immune functions.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Doleschall M,Mayer B,Cervenak J,Cervenak L,Kacskovics Idoi
10.1016/j.dci.2006.12.007subject
Has Abstractpub_date
2007-01-01 00:00:00pages
945-61issue
9eissn
0145-305Xissn
1879-0089pii
S0145-305X(07)00004-3journal_volume
31pub_type
杂志文章abstract：:To date, functioning of insect humoral immune response is especially well described in Diptera. The mechanisms of pathogen recognition, activation of signaling pathways and regulation of antimicrobial defense peptide expression are relatively well known. The present paper demonstrates evidence that the immune system o...
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