Abstract:
:CfTEP is a member of thioester-containing protein (TEP) family found in Zhikong scallop Chlamys farreri and is involved in innate immunity against invading microbes. In the present study, the genomic DNA of CfTEP was cloned and characterized. The genomic DNA sequence of CfTEP consisted of 40 exons and 39 introns spanning 35kb with all exon-intron junction sequences agreeing with the GT/AG consensus. The genomic organization of CfTEP was similar to human and mouse C3 rather than ciona C3-1 and Drosophila dTEP2. By RT-PCR technique, seven different cDNA variants of CfTEP (designated as CfTEP-A-CfTEP-G) were cloned from scallop gonad. CfTEP-A-CfTEP-F were produced by alternative splicing of six mutually exclusive exons (exons 19-24), respectively, which encoded the highly variable central region. While in CfTEP-G, the deletion of all the six exons introduced a new translation stop site and might trigger nonsense mediated decay (NMD). The mRNA expression and the proportion of the seven CfTEP variant transcripts were examined in the gonad of scallops after bacterial challenge. The fragments containing the highly variable central region of CfTEP were amplified by RT-PCR and a 100 positive clones were sequenced randomly. The expression profiles of the seven CfTEP variants were different and displayed the sex and bacteria dependent manner. In the blank, sea water and Listonella anguillarum challenged subgroups of male scallops, all the transcripts detected were CfTEP-G isoform. In the Micrococcus luteus challenged subgroup, the isoforms expressed and their proportions were CfTEP-F (54%), CfTEP-B (23%), CfTEP-A (10%), CfTEP-C (7%) and CfTEP-E (6%). However, in the gonad of female scallops, only CfTEP-A were found in blank and sea water challenged subgroups. After L. anguillarum or M. luteus challenge, four and five isoforms were detected, respectively, with CfTEP-F isoform being the most one in the both subgroups. These results suggested that the evolution of TEP genes was very complex, and that the diverse CfTEP transcripts generated by alternative splicing played an important role as pattern recognition receptors in the innate immune defense of scallops.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Zhang H,Wang L,Song L,Zhao J,Qiu L,Gao Y,Song X,Li L,Zhang Y,Zhang Ldoi
10.1016/j.dci.2009.05.007subject
Has Abstractpub_date
2009-10-01 00:00:00pages
1070-6issue
10eissn
0145-305Xissn
1879-0089pii
S0145-305X(09)00107-4journal_volume
33pub_type
杂志文章abstract::Serine protease inhibitors (SPIs) play a key role in physiological responses by controlling protease activities. In this study, we studied the biochemical functions of serpin-15, an SPI, from Bombyx mori (Bmserpin-15). Recombinant Bmserpin-15 was expressed in Escherichia coli cells and used to raise rabbit anti-Bmserp...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2015.02.013
更新日期:2015-07-01 00:00:00
abstract::A range of marine invertebrates were screened for prophenoloxidase (a marker protein of the prophenoloxidase activating system) in the coelomic fluid or hemolymph. The crustaceans and the ascidian, Ciona intestinalis, displayed strongest activity, with low levels found in the starfish, Asterias rubens, the sea urchin,...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(91)90018-t
更新日期:1991-10-01 00:00:00
abstract::Ovotransferrin (OTF) is an acute phase protein in chickens, serum levels of which increase in inflammation and infections. To understand the significance of OTF in inflammation, we studied its in vitro effects on HD11 cells, a macrophage cell line, and heterophils isolated from blood using a panel of variables indicat...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/s0145-305x(02)00028-9
更新日期:2002-11-01 00:00:00
abstract::Receptor-interacting serine/threonine protein kinase 1 (RIPK1) is an important regulator of necroptosis and involved in innate immune response in human and mammal; however, its function in teleost fish mains largely unknown. In this paper, the RIPK1 homologue of black carp (Mylopharyngodon piceus) has been cloned and ...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2020.103726
更新日期:2020-08-01 00:00:00
abstract::In order to investigate the effects of TNFalpha upon the growth of fish thymocytes, rainbow trout thymocytes were cultured in the conditioned medium (CM): the supernatants of the macrophage cultures stimulated with chitin derivative and LPS. Synthesis of TNFalpha by macrophages and subsequent secretion into CM were as...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2005.09.005
更新日期:2006-01-01 00:00:00
abstract::Peroxinectin possesses the features of both peroxidase activity and adhesive property and plays important roles in innate immune system of crustaceans. In this study, the sequence of peroxinectin of Eriocheir sinensis (EsPX) was analyzed and its expression in response to exterior stimulation was detected in both in vi...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2015.02.016
更新日期:2015-07-01 00:00:00
abstract::The cartilaginous fish (chimeras, sharks, skates and rays) are the oldest group relative to mammals in which an adaptive immune system founded upon immunoglobulins has been found. In this manuscript we characterize the immunoglobulins of the spiny dogfish (Squalus acanthias) at both the molecular and expressed protein...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2011.10.007
更新日期:2012-04-01 00:00:00
abstract::Few pathogens have shaped human medicine as the mycobacteria. From understanding biological phenomena driving disease spread, to mechanisms of host-pathogen interactions and antibiotic resistance, the Mycobacterium genus continues to challenge and offer insights into the basis of health and disease. Teleost fish model...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章,评审
doi:10.1016/j.dci.2019.02.011
更新日期:2019-07-01 00:00:00
abstract::Bcl-2-associated X (Bax) belongs to the Bcl-2 protein family and its pro-apoptotic function has been confirmed in many vertebrate species. However, the functional role of Bax in apoptosis in invertebrates is limited. Here, a Bax homologue (AjBax) in Apostichopus japonicas was cloned and characterized, and its pro-apop...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2019.103490
更新日期:2020-01-01 00:00:00
abstract::The prevalence of pathogenic viruses is a serious issue as they pose a constant threat to both the poultry industry and to human health. To prevent these viral infections an understanding of the host-virus response is critical, especially for the development of novel therapeutics. One approach in the control of viral ...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章,评审
doi:10.1016/j.dci.2013.05.020
更新日期:2013-11-01 00:00:00
abstract::Molecular phylogenetics and its applications are popular and useful tools for making comparative investigations in genetics; however, estimating phylogenetic trees is not always straightforward. Some phylogenetic estimators use an explicit model of nucleotide evolution to estimate evolutionary parameters such as branc...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章,评审
doi:10.1016/j.dci.2004.07.007
更新日期:2005-01-01 00:00:00
abstract::Studies were conducted to determine the ability of leukocytes from the nurse shark to migrate in an in vitro micropore filter chemotaxis assay and to determine optimal assay conditions and suitable attractants for such an assay. A migratory response was seen with several attractants: activated rat serum, activated sha...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(85)90113-2
更新日期:1985-04-01 00:00:00
abstract::Stimulator of interferon gene (STING), an important adapter responsible for RLR pathway, plays a pivotal role in both viral RNA- and DNA-triggered induction of IFNs in mammals. To understand the roles of STING in piscine immune system, STING gene (CiSTING) was identified from grass carp (Ctenopharyngodon idella). The ...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2014.03.001
更新日期:2014-07-01 00:00:00
abstract::Allorecognition has been well-studied in the context of vertebrate adaptive immunity and recognition of the Major Histocompatibility Complex (MHC), which is the central event of vertebrate immune responses. Although allorecognition systems have been identified throughout the metazoa, recent results have shown that the...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章,评审
doi:10.1016/j.dci.2014.03.014
更新日期:2015-01-01 00:00:00
abstract::The human IL-1 receptor family is comprised of 11 membrane bound or soluble receptors and the IL-18 binding protein (IL-18BP). These receptors are dispersed across seven genomic loci, with the majority at a single locus. Direct orthologues were identified in the chicken at conserved genomic loci; however, the IL-18BP ...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2020.103850
更新日期:2021-01-01 00:00:00
abstract::Splenic suppressor cells from neonatal ("intrinsic") and adult (tumor-allosensitized) mice were compared. Both are non-antigen specific and non-H-2 restricted, and are Ly1+2+, I-J+, QA2+ T-cells. Injection of adult peritoneal exudate cells into neonates or adult tumor-allosensitized mice decreases suppressor cell acti...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(85)90066-7
更新日期:1985-01-01 00:00:00
abstract::Ascorbate is an essential nutrient commonly regarded as an antioxidant. In this study, using axenic brine shrimp and pathogenic strain Vibrio harveyi as the host-pathogen model, we confirmed that pretreatment of sodium ascorbate (NaAs), at an optimum concentration, was a prooxidant by generation of hydrogen peroxide, ...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2018.11.007
更新日期:2019-03-01 00:00:00
abstract::The full length coding sequence of the cattle transcription factor p65 was isolated and cloned. The cloned bovine p65 was expressed in mammalian cells, and it induced the NF-kappaB-specific luciferase reporter gene expression. Using gel retardation techniques, we demonstrated that the cloned bovine p65 bound to the co...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2006.12.007
更新日期:2007-01-01 00:00:00
abstract::Interferon (IFN) regulatory factor 3 (IRF3) is a major regulator contributing to the host away from viral infection. Here, an IRF3 gene from sea perch (LjIRF3) was identified and its role in regulating early apoptosis signaling and IFN response was investigated during red spotted grouper nervous necrosis virus (RGNNV)...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2018.07.014
更新日期:2018-11-01 00:00:00
abstract::Yellow head virus (YHV) is one of the most widespread viruses seriously affecting black tiger shrimp (Penaeus monodon) cultivation. A previous microarray study demonstrated that clathrin coat assembly protein 17 (AP17) was significantly up-regulated after YHV infection (Pongsomboon et al., 2011). Clathrin coat AP17 is...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2013.11.017
更新日期:2014-05-01 00:00:00
abstract::Two clones, designated Icpu-UA/3 and Icpu-UA/26, were isolated from a genomic library prepared from a single homozygous gynogenetic channel catfish. Sequence analysis showed that each clone encoded a gene product containing features conserved among MHC class I molecules. The genomic organization of both clones indicat...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/s0145-305x(01)00017-9
更新日期:2001-09-01 00:00:00
abstract::Rab genes, encoding a large family of monomeric G-proteins, contain over 60 members in the human genome. They have been recognized as crucial regulators for membrane trafficking including cargo sorting, vesicle formation, budding, motility, docking, fusion, secretory and endocytic pathway of host immune responses. How...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2014.01.026
更新日期:2014-07-01 00:00:00
abstract::The enzyme phenoloxidase is a critical component of the immunological defence of invertebrates. Previously, we have shown that the activity of phenoloxidase in Sydney rock oysters (Saccostrea glomerata) correlates with the severity of QX disease outbreaks. The aetiological agent of QX disease is the opportunistic prot...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2007.06.006
更新日期:2008-01-01 00:00:00
abstract::We analyzed the effects of infection with Trichobilharzia ocellata on hemocytes of its snail host, Lymnaea stagnalis, and correlated them with successive stages of parasite development. Circulating hemocytes were studied at 0, 2, 4, 6, and 8 weeks post exposure (p.e.) with respect to cell number, distribution of subpo...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(91)90001-f
更新日期:1991-04-01 00:00:00
abstract::A bovine interferon-kappa (BoIFN-κ) gene was amplified, which encodes a protein of 215 amino acids sharing 63% identity with human IFN-κ. BoIFN-κ was demonstrated to have antiviral and antiproliferative activities. Moreover, BoIFN-κ was shown to be highly sensitive to trypsin, however, it remained stable despite chang...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2016.09.018
更新日期:2017-02-01 00:00:00
abstract::Dengue virus has become one of the most important arboviral pathogens affecting the world today. The virus is transmitted among humans by the mosquitoes Aedes aegypti and Ae. albopictus. Like other vector-borne pathogens, this virus encounters innate immune defenses within the mosquito vector that limit infection. We ...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2010.01.006
更新日期:2010-06-01 00:00:00
abstract::Phagocytosis is main cellular immunity, however, it is still unknown or debated upon which types of hemocyte contributes phagocytosis in penaeid shrimps. The hemocyte characterization in kuruma shrimp have been mainly performed based on its morphology by microscopic observation. Therefore, establishment of molecular m...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2017.12.014
更新日期:2018-04-01 00:00:00
abstract::We have purified five types of lectins, named tachylectins, from circulating hemocytes and hemolymph plasma of the Japanese horseshoe crab, Tachypleus tridentatus. Tachylectin-1 interacts with Gram-negative bacteria probably through 2-keto-3-deoxyoctonate, one of the constituents of lipopolysaccharides (LPS). Tachylec...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章,评审
doi:10.1016/s0145-305x(99)00019-1
更新日期:1999-06-01 00:00:00
abstract::In this study, we examined the expression of epithelial Na(+) channel (ENaC) subunits in human peripheral blood lymphocytes, human lymph nodes and molluscan immunocytes using non-radioactive in situ hybridization. The results showed that T lymphocytes express the ENaC gamma subunit mRNA, and B lymphocytes the ENaC bet...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/s0145-305x(01)00097-0
更新日期:2002-06-01 00:00:00
abstract::Common carp thrombocytes account for 30-40% of peripheral blood leukocytes and are abundant in the healthy animals' spleen, the thrombopoietic organ. We show that, ex vivo, thrombocytes from healthy carp express a large number of immune-relevant genes, among which several cytokines and Toll-like receptors, clearly poi...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2015.02.008
更新日期:2015-06-01 00:00:00