Abstract：
:Bcl-2-associated X (Bax) belongs to the Bcl-2 protein family and its pro-apoptotic function has been confirmed in many vertebrate species. However, the functional role of Bax in apoptosis in invertebrates is limited. Here, a Bax homologue (AjBax) in Apostichopus japonicas was cloned and characterized, and its pro-apoptotic function explored. In healthy sea cucumbers, AjBax was expressed in coelomocyte with the highest levels. AjBax mRNA and protein levels were significantly induced in coelomocytes post Vibrio splendidus challenge in vivo and LPS-exposed in vitro. Moreover, siRNA-mediated AjBax knockdown in coelomocyte significantly decreased AjBax mRNA and protein levels as well as the apoptosis levels of coelomocyte. Furthermore, AjBax protein levels and coelomocyte apoptosis levels could be partially recovered to their original levels after supplementation with recombinant AjBax. Our results support that AjBax has a similar function to Bax proteins in vertebrates and that it may serve as a pro-apoptotic regulator in sea cucumbers.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Guo M,Lv M,Shao Y,Zhang W,Zhao X,Li Cdoi
10.1016/j.dci.2019.103490subject
Has Abstractpub_date
2020-01-01 00:00:00pages
103490eissn
0145-305Xissn
1879-0089pii
S0145-305X(19)30363-5journal_volume
102pub_type
杂志文章abstract：:Cellular and humoral aspects of the immune response develop sequentially in the fetus. During the ontogeny, the pluripotent stem cells emerge and differentiate into all hematopoietic lineages. Basic questions including the identification of the first lympho-hematopoietic sites, the origin of T and B lymphocytes, the d...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章,评审
doi：10.1016/j.dci.2008.07.011
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abstract：:We describe a novel antigen recognized by mAb 2E3 selectively expressed in the periphery by a subset of porcine CD4+ T cells. Both, CD4+CD8alpha- and CD4+CD8alphalow T cell subpopulations express this antigen. CD4+2E3+ T cells show phenotypical and functional characteristics of nai;ve cells. The majority of them are C...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/s0145-305x(03)00137-x
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abstract：:Macrophages are the most functionally heterogenous cells of the hematopoietic system. Given many diseases are underpinned by inappropriate macrophage activation, macrophages have emerged as a therapeutic target to treat disease. A thorough understanding of what controls macrophage activation will likely reveal new pat...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2015.06.013
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abstract：:Experiments were conducted to determine the ability of the protistan parasite, Perkinsus marinus, to inhibit chemiluminescence of hemocytes from the eastern oyster, Crassostrea virginica. Luminol-enhanced chemiluminescence (CL) was used to measure the production of reactive oxygen intermediates (ROI) generated by oyst...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(94)00059-o
更新日期：1995-03-01 00:00:00
abstract：:The CD28 molecule, a disulfide-linked homodimer expressed on peripheral T cells and thymocytes, mediates an essential costimulatory signal following engagement of the T cell receptor (TCR). Increased proportions of CD28- T cells have been observed during aging and in situations of chronic immune stimulation, but the o...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/s0145-305x(97)00027-x
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abstract：:Complement components C3 and C4 have been isolated from the serum of the nurse shark (Ginglymostoma cirratum) and of the channel catfish (Ictalurus punctatus). As in the higher vertebrates, the fish C4 proteins have three-chain structures while the C3 proteins have two-chain structures. All four proteins have intra-ch...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/s0145-305x(98)00002-0
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/s0145-305x(02)00119-2
更新日期：2003-05-01 00:00:00
abstract：:The snail Biomphalaria glabrata kills the blood fluke Schistosoma mansoni by a mechanism involving production of hydrogen peroxide, the enzymatic product of cytosolic Cu/Zn superoxide dismutase (SOD1). This enzyme exhibits higher activity in blood cells (hemocytes) from a predominantly resistant strain of B. glabrata ...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2006.12.005
更新日期：2007-01-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2009.08.005
更新日期：2010-01-01 00:00:00
abstract：:The dog is both a valued veterinary species and a widely used translational model for sepsis research. However, relatively little work has been performed evaluating potential biomarkers present during canine infection. Triggering receptor expressed on myeloid cells-1 (TREM-1) has shown promise as a biomarker for infec...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2011.03.021
更新日期：2011-08-01 00:00:00
abstract：:An in vitro method for organ culture of embryonic bursa of Fabricius is presented. It is shown that bursa cells proliferate in in vitro culture as evidenced by [3H]-thymidine incorporation. We assessed the expression of B-cell alloantigen (Bu-la and Bu-lb), class I (B-F) and class II (B-L) antigens of chicken major hi...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(90)90095-v
更新日期：1990-04-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2012.05.003
更新日期：2012-10-01 00:00:00
abstract：:In recent years zebrafish has emerged as an excellent model for studying the Cxcl8 signaling pathway in inflammation elicited upon tissue damage or infection. Zebrafish has two true homologs of mammalian CXCL8, named Cxcl8-l1 and Cxcl8-l2. Previously, we have shown that in wound-associated inflammation, these chemokin...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2014.11.004
更新日期：2015-03-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2005.09.003
更新日期：2006-01-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2020.103757
更新日期：2020-10-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(93)90014-h
更新日期：1993-01-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章,评审
doi：10.1016/j.dci.2004.07.007
更新日期：2005-01-01 00:00:00
abstract：:Interleukin 1b (IL-1b) is a member of the cytokine family that serves as major mediators for early pro-inflammatory responses. During artificial breeding of Pacific cod (Gadus macrocephalus), outbreaks of NNV in the early development stages could lead to high mortality. Recent research reported that IL-1b participated...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2018.07.025
更新日期：2018-11-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2014.10.006
更新日期：2015-01-01 00:00:00
abstract：:Porcine reproductive and respiratory syndrome virus (PRRSV) is an emerging animal virus that has caused high economic losses for the swine industry worldwide. Previous in vitro and in vivo studies demonstrated that PRRSV infection induces significant production of interleukin 10 (IL-10), a pleiotropic cytokine with im...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2012.10.001
更新日期：2013-03-01 00:00:00
abstract：:Phenoloxidase (PO) plays an important role in arthropod melanization. Previously, a prophenoloxidase (PmproPO1) gene was cloned and characterized from the hemocytes of the black tiger shrimp, Penaeus monodon. In the present study, we report a novel proPO gene (PmproPO2) belonging to the proPO family identified from th...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2008.09.003
更新日期：2009-02-01 00:00:00
abstract：:To date two closely related interleukin-1β genes (IL-1β1 and IL-β2) have been found in salmonids. The cloning of trout and salmon IL-1β3, and a salmon IL-1β4 pseudogene reveals that two types of IL-1β genes exist in teleost species. Type I teleost IL-1β genes, including salmonid IL-1β3, share a similar 6 coding exon s...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2012.07.010
更新日期：2012-11-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/s0145-305x(02)00028-9
更新日期：2002-11-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(84)90102-2
更新日期：1984-07-01 00:00:00
abstract：:Infectious bursal disease (IBD) is an economically important immunosuppressive disease of chickens. The IBD virus (IBDV) actively replicates in B cells and causes severe bursal damage. Generally, T cells are refractory to infection with IBDV but are known to promote virus clearance. However, the mechanisms of T cell m...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2011.01.007
更新日期：2011-05-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
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更新日期：2020-12-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/s0145-305x(01)00027-1
更新日期：2002-01-01 00:00:00
abstract：:Corals form the framework of the world's coral reefs and are under threat from increases in disease and bleaching (symbiotic dysfunction), yet the mechanisms of pathogen and symbiont recognition remain largely unknown. Here we describe the isolation and characterisation of an ancient mannose-binding lectin in the cora...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2008.05.010
更新日期：2008-01-01 00:00:00
abstract：:Kazal-type serine proteinase inhibitors (KPIs) function in physiological and immunological processes requiring proteinase action. In the present study, the first Cherax quadricarinatus KPI gene (designated CqKPI) was identified and characterized. The open reading frame of CqKPI contains 405 nucleotides and encodes a p...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2020.103871
更新日期：2021-01-01 00:00:00
abstract：:Toll-like receptors (TLRs) play an important role in the activation of innate and adaptive immune responses. Stimulation with multiple TLR agonists may result in synergistic, complimentary or inhibitory effects on innate immune responses. In this study, we investigated the effects of co-stimulation of sheep peripheral...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2009.12.018
更新日期：2010-05-01 00:00:00
