Abstract：
:The RIG-I like helicases, RIG-I, mda5 and LGP2 are an evolutionarily conserved family of cytosolic pattern recognition receptors important in the recognition of viral RNA, and responsible for the innate induction of interferons and proinflammatory cytokines upon viral infection. Bats are natural reservoir hosts to a variety of RNA viruses that cause significant morbidity and mortality in other species; however the mechanisms responsible for the control of viral replication in bats are not understood. This report describes the molecular cloning and expression analysis of RIG-I, mda5 and LGP2 genes in the fruit bat Pteropus alecto, and is the first description of RIG-I like helicases from any species of bat. Our results demonstrate that P. alecto RIG-I, mda5 and LGP2 have similar primary structures and tissue expression patterns to their counterparts in humans and other mammals. Stimulation of bat kidney cells with synthetic dsRNA (poly I:C) induced high levels of interferon β and rapid upregulation of all three helicases. These findings reveal that the cytoplasmic virus sensing machinery is present and intact in P. alecto. This study provides the foundation for further investigations into the interactions between bat RIG-I-like helicases and viruses to elucidate the mechanisms responsible for the asymptomatic nature of viral infections in bats.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Cowled C,Baker ML,Zhou P,Tachedjian M,Wang LFdoi
10.1016/j.dci.2011.11.008subject
Has Abstractpub_date
2012-04-01 00:00:00pages
657-64issue
4eissn
0145-305Xissn
1879-0089pii
S0145-305X(11)00308-9journal_volume
36pub_type
杂志文章abstract：:In recent years, several studies have demonstrated the existence of cytokine-like molecules in invertebrates, therefore suggesting that cytokines may have been conserved throughout evolution. In this study, we investigated the presence of immunoreactive TNF alpha (ir TNF alpha) in the gastropod mollusc Biomphalaria gl...
journal_title：Developmental and comparative immunology
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journal_title：Developmental and comparative immunology
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journal_title：Developmental and comparative immunology
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pub_type： 杂志文章
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journal_title：Developmental and comparative immunology
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journal_title：Developmental and comparative immunology
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更新日期：2015-08-01 00:00:00
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更新日期：2010-06-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2011.09.008
更新日期：2012-03-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
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更新日期：1994-05-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
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更新日期：2012-04-01 00:00:00
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pub_type： 杂志文章
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
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更新日期：2016-12-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
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更新日期：2018-04-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 历史文章,杂志文章,评审
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
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更新日期：2008-01-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章,评审
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journal_title：Developmental and comparative immunology
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章,评审
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更新日期：2015-01-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
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更新日期：2003-06-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
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更新日期：2021-01-01 00:00:00
