Abstract:
:C-type lectins participate in pathogen recognition and other defense responses in innate immunity as well as in cell-cell interactions. A new cDNA encoding a 335-residue polypeptide containing two tandem C-type lectin domains was cloned from the haemocytes of Helicoverpa armigera (Ha-lectin). Northern hybridizations revealed that the mRNA of Ha-lectin was expressed constitutively in haemocytes, and was up-regulated following injections of bacteria, yeast, or virus. Ha-lectin expression was also induced in the fat body when larvae were injected with bacteria, yeast or 20-hydroxyecdysone and a non-steroidal ecdysone agonist, RH-2485. The Ha-lectin was detected in granular haemocytes. The recombinant protein (rHa-lectin) expressed in Escherichia coli had hemagglutinating and sugar-binding activities. The native Ha-lectin protein was identified in haemocytes and plasma using a polyclonal antiserum raised against rHa-lectin by immunoblotting techniques. All together, our results suggest that the Ha-lectin gene is involved in innate immunity, and may also participate in the molting process.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Chai LQ,Tian YY,Yang DT,Wang JX,Zhao XFdoi
10.1016/j.dci.2007.04.006subject
Has Abstractpub_date
2008-01-01 00:00:00pages
71-83issue
1eissn
0145-305Xissn
1879-0089pii
S0145-305X(07)00073-0journal_volume
32pub_type
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