Abstract：
:In all vertebrates studied to date, the expression of MHC class II genes is known to be restricted to a limited number of tissues and cell types. In order to have a better understanding of the function of the equivalent genes in teleost fish, the distribution of MHC class II beta transcripts (Cyca-DAB) in the common carp (Cyprinus carpio L.) was investigated. RNA was isolated from tissues and leucocytes, cDNA was produced, and amplification of the Cyca-DAB genes was carried out by PCR. Of the organs with known immunological function, the highest level of Cyca-DAB transcription was found in the thymus. Despite their expected different cellular organization, total blood, head kidney, spleen and the second segment of the gut had similar Cyca-DAB expression levels. No class II transcripts were detected in the skeletal muscle. The studies carried out with leucocytes isolated from the lymphoid tissues point to a direct correlation between the levels of expression and the amounts of surface immunoglobulin positive (sIg+) cells present in the different cell fractions. However, thymus leucocytes did not follow this correlation since the highest level of class II expression was found in a thymocyte fraction that contained very low numbers of Ig+ cells. In PBL the Ig+ cells were highly positive whereas the Ig- were weakly positive. Adherent leucocytes shown to be class II positive, although adherent cells from PBL show a lower level of expression compared to those from the spleen and head kidney.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Rodrigues PN,Hermsen TT,Rombout JH,Egberts E,Stet RJdoi
10.1016/0145-305x(95)00033-psubject
Has Abstractpub_date
1995-11-01 00:00:00pages
483-96issue
6eissn
0145-305Xissn
1879-0089pii
0145305X9500033Pjournal_volume
19pub_type
杂志文章abstract：:Toll-like Receptors (TLRs) are the first innate receptors in recognizing pathogen-associated molecular patterns. In fish, upregulation of toll-like receptors during infection has been largely demonstrated while the effects of abiotic stressors on their expression remain poorly investigated. In this study, striped catf...
journal_title：Developmental and comparative immunology
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journal_title：Developmental and comparative immunology
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journal_title：Developmental and comparative immunology
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章,评审
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更新日期：2017-10-01 00:00:00
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journal_title：Developmental and comparative immunology
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doi：10.1016/j.dci.2004.10.001
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pub_type： 杂志文章
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2010.06.006
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pub_type： 杂志文章,评审
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更新日期：2011-09-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(87)90047-4
更新日期：1987-07-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
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更新日期：2012-04-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
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更新日期：2007-01-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2014.07.007
更新日期：2014-11-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
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journal_title：Developmental and comparative immunology
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journal_title：Developmental and comparative immunology
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2009.02.007
更新日期：2009-07-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2012.11.003
更新日期：2013-03-01 00:00:00
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journal_title：Developmental and comparative immunology
pub_type： 杂志文章,评审
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更新日期：2016-05-01 00:00:00
abstract：:Dipteran insects, like mosquitoes, possess more than two prophenoloxidase (PPO) genes, but it is unclear whether their gene products differ in biochemical properties and physiological functions. Here, we used three Drosophila melanogaster PPOs as models to study their properties through expression in S2 cells. Our dat...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2011.12.001
更新日期：2012-03-01 00:00:00
abstract：:The interferon regulatory factor (IRF) with its 10 members is a very important gene family related to innate immunity. Currently, most fish IRFs reported are from bony fish (teleosts). Cloning and sequencing of IRFs from chondrosteans, the so-called "ancient fish" including sturgeon, paddlefish, bichir and gar, are ab...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2011.06.003
更新日期：2012-01-01 00:00:00
abstract：:Anti-lipopolysaccharide factor (ALF) is one of the widely-studied antimicrobial peptides (AMPs) with broad-spectrum antibacterial activity and antiviral property. Previous studies show the existence of multiform of ALFs in crustacean which are important for immunity of the animals. In the present study, we characteriz...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2014.05.013
更新日期：2014-10-01 00:00:00
abstract：:We analyzed the effects of infection with Trichobilharzia ocellata on hemocytes of its snail host, Lymnaea stagnalis, and correlated them with successive stages of parasite development. Circulating hemocytes were studied at 0, 2, 4, 6, and 8 weeks post exposure (p.e.) with respect to cell number, distribution of subpo...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/0145-305x(91)90001-f
更新日期：1991-04-01 00:00:00
abstract：:Beta_defensin have been solely found in vertebrates until β-defensin-like peptides were described as transcript isoforms in two species of Panulirus genus. They were considered as putative antimicrobials since their biological activity have not been demonstrated. Here we purified and characterized a defensin-like pept...
journal_title：Developmental and comparative immunology
pub_type： 杂志文章
doi：10.1016/j.dci.2016.09.002
更新日期：2017-02-01 00:00:00
