当前位置： SCI文献检索 > DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY期刊下所有文献 > Two recombinant peptides, SpStrongylocins 1 and 2, from Strongylocentrotus purpuratus, show antimicrobial activity against Gram-positive and Gram-negative bacteria.

Two recombinant peptides, SpStrongylocins 1 and 2, from Strongylocentrotus purpuratus, show antimicrobial activity against Gram-positive and Gram-negative bacteria.

Abstract：

:The cysteine-rich strongylocins were the first antimicrobial peptides (AMPs) discovered from the sea urchin species, Strongylocentrotus droebachiensis. Homologous putative proteins (called SpStrongylocin) were found in the sister species, S. purpuratus. To demonstrate that they exhibit the same antibacterial activity as strongylocins, cDNAs encoding the 'mature' peptides (SpStrongylocins 1 and 2) were cloned into a direct expression system fusing a protease cleavage site and two purification tags to the recombinant peptide. Both recombinant fusion peptides were expressed in a soluble form in an Escherichia coli strain tolerant to toxic proteins. Enterokinase was used to remove the fusion tags and purified recombinant SpStrongylocins 1 and 2 showed antimicrobial activity against both Gram-negative and Gram-positive bacteria. The results of membrane integrity assays against cytoplasmic membranes of E. coli suggest that both recombinant SpStrongylocins 1 and 2 conduct their antibacterial activity by intracellular killing mechanisms because no increase in membrane permeability was detected.

journal_name

Dev Comp Immunol

journal_title

Developmental and comparative immunology

authors

Li C,Blencke HM,Smith LC,Karp MT,Stensvåg K

doi

10.1016/j.dci.2009.10.006

subject

Has Abstract

pub_date

2010-03-01 00:00:00

pages

286-92

issue

3

eissn

0145-305X

issn

1879-0089

pii

S0145-305X(09)00220-1

journal_volume

34

pub_type

杂志文章

相关文献

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY文献大全
  • Development of the immune system and immunological protection in marsupial pouch young.

    abstract：:At birth the tissues of marsupial immune system are underdeveloped. The young animal is not immunocompetent. Histological and immunohistochemical studies of pouch young epithelial tissues provide a clear picture of tissue development but the timing of onset of immunocompetence awaits definition. The survival of the ne...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章,评审

    doi：10.1016/s0145-305x(00)00008-2

    authors： Old JM,Deane EM

    更新日期：2000-07-01 00:00:00

  • Loss of CD28 expression on T lymphocytes: a marker of replicative senescence.

    abstract：:The CD28 molecule, a disulfide-linked homodimer expressed on peripheral T cells and thymocytes, mediates an essential costimulatory signal following engagement of the T cell receptor (TCR). Increased proportions of CD28- T cells have been observed during aging and in situations of chronic immune stimulation, but the o...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/s0145-305x(97)00027-x

    authors： Effros RB

    更新日期：1997-11-01 00:00:00

  • ProPhenolOxidase in Daphnia magna: cDNA sequencing and expression in relation to resistance to pathogens.

    abstract：:Invertebrates utilise the innate immune system when defending against pathogenic attack. However, except for some effectors as proPhenolOxidase (proPO), the innate immune response is less well understood outside model insect species, and its role in natural host-pathogen systems is generally not well documented. We ha...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2008.11.012

    authors： Labbé P,Little TJ

    更新日期：2009-05-01 00:00:00

  • Damselfish with neurofibromatosis exhibit cytotoxicity towards retrovirus infected cells.

    abstract：:Lymphocytes from tumor-bearing damselfish are cytotoxic towards target cell lines derived from damselfish neurofibromatosis. These cell lines contain at least one retrovirus which appears to be related to the etiology of the disease. The current studies were designed to characterize the effectors of this cytotoxic rea...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/s0145-305x(97)00010-4

    authors： McKinney EC,Schmale MC

    更新日期：1997-05-01 00:00:00

  • High doses of sodium ascorbate act as a prooxidant and protect gnotobiotic brine shrimp larvae (Artemia franciscana) against Vibrio harveyi infection coinciding with heat shock protein 70 activation.

    abstract：:Ascorbate is an essential nutrient commonly regarded as an antioxidant. In this study, using axenic brine shrimp and pathogenic strain Vibrio harveyi as the host-pathogen model, we confirmed that pretreatment of sodium ascorbate (NaAs), at an optimum concentration, was a prooxidant by generation of hydrogen peroxide, ...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2018.11.007

    authors： Han B,Kaur VI,Baruah K,Nguyen VD,Bossier P

    更新日期：2019-03-01 00:00:00

  • A developmental investigation of the liver, bone marrow and spleen of the stripe-faced dunnart (Sminthopsis macroura).

    abstract：:The development of the liver, bone marrow and spleen have been investigated in the stripe-faced dunnart. At birth, the liver was undergoing haematopoiesis but the level declined rapidly and by day 50 after birth the liver was histologically mature. Both the bone marrow and spleen were non-haematopoietic at birth but i...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2003.08.004

    authors： Old JM,Selwood L,Deane EM

    更新日期：2004-04-01 00:00:00

  • The Drosophila Thioester containing Protein-4 participates in the induction of the cellular immune response to the pathogen Photorhabdus.

    abstract：:The function of thioester-containing proteins (TEPs) in the immune defense of the fruit fly Drosophila melanogaster is yet mostly unexplored. Recently, we showed the involvement of TEP4 in the activation of humoral and phenoloxidase immune responses of Drosophila against the pathogenic bacteria Photorhabdus luminescen...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2017.06.008

    authors： Shokal U,Eleftherianos I

    更新日期：2017-11-01 00:00:00

  • Determinants of defence strategies of a hibernating European bat species towards the fungal pathogen Pseudogymnoascus destructans.

    abstract：:Pseudogymnoascus destructans (Pd), the causative agent of white-nose syndrome in North America, has decimated bat populations within a decade. The fungus impacts bats during hibernation when physiological functions, including immune responses, are down-regulated. Studies have shown that Pd is native to Europe, where i...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2021.104017

    authors： Fritze M,Puechmaille SJ,Costantini D,Fickel J,Voigt CC,Czirják GÁ

    更新日期：2021-01-18 00:00:00

  • Correlated expression profile of extracellular matrix-related molecules during the inflammatory response of the teleost fish gilthead seabream.

    abstract：:Extracellular matrix (ECM) components, in addition to their structural functions, interact with cell surface receptors and intracellular components to modulate the transduction of signals for cell growth, differentiation, migration, proliferation, polarization, apoptosis and inflammation. Our previous findings in the ...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2010.05.007

    authors： Castillo-Briceño P,Arizcun-Arizcun M,Meseguer J,Mulero V,García-Ayala A

    更新日期：2010-10-01 00:00:00

  • Evolution of low molecular weight immunoglobulins. V. Degree of antigenic relationship between the 7S immunoglobulins of mammals, birds, and lower vertebrates to the turkey IgY.

    abstract：:There are close antigenic relations between the turkey IgY--the dominant 7S Ig of serum--and the corresponding IgY-like Igs of chicken, duck, goose, tortoise, and frog as well as serum or colostral IgA of man and swine. The RIA-systems used for the determination of immunological cross-reactivity among the Ig types con...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/0145-305x(86)90027-3

    authors： Hädge D,Ambrosius H

    更新日期：1986-07-01 00:00:00

  • Alternate forms of MHC class II-associated invariant chain are not produced by alternative splicing in rainbow trout (Oncorhynchus mykiss) but are encoded by separate genes.

    abstract：:A major limiting factor in understanding teleost major histocompatibility receptor function is the lack of knowledge about antigen presentation accessory molecules. We report here two cDNA clones encoding teleost versions of invariant chain and one encoding a related protein that may play a protease inhibition role in...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/s0145-305x(02)00119-2

    authors： Fujiki K,Smith CM,Liu L,Sundick RS,Dixon B

    更新日期：2003-05-01 00:00:00

  • Analysis of the Atlantic salmon genome reveals a cluster of Mx genes that respond more strongly to IFN gamma than to type I IFN.

    abstract：:Mx proteins are antiviral GTPases, which are induced by type I IFN and virus infection. Analysis of the Atlantic salmon genome revealed the presence of 9 Mx genes localized to three chromosomes. A cluster of three Mx genes (SsaMx1 - SsaMx3), which includes previously cloned Mx genes, is present on chromosome (Chr) 12....

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2018.09.004

    authors： Robertsen B,Greiner-Tollersrud L,Jørgensen LG

    更新日期：2019-01-01 00:00:00

  • TNF induces the growth of thymocytes in rainbow trout.

    abstract：:In order to investigate the effects of TNFalpha upon the growth of fish thymocytes, rainbow trout thymocytes were cultured in the conditioned medium (CM): the supernatants of the macrophage cultures stimulated with chitin derivative and LPS. Synthesis of TNFalpha by macrophages and subsequent secretion into CM were as...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2005.09.005

    authors： Hino K,Nakamura O,Yoshiura Y,Suetake H,Suzuki Y,Watanabe T

    更新日期：2006-01-01 00:00:00

  • Molecular identification and expression analysis of TAB1 from orange-spotted grouper (Epinephelus coioides).

    abstract：:Transforming growth factor-β activated kinase 1 (TAK1) is a crucial signal transducer in multiple signaling pathways. TAK1 binds TAB1, TAB2, and TAB3, which act as activators and adaptors that specifically regulate the activation of TAK1. To date, the role of TABs is largely unknown in fish. In the present study, a TA...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2018.09.014

    authors： Hu YZ,Li X,Han R,Jiang B,Li YW,Dan XM,Li AX

    更新日期：2019-01-01 00:00:00

  • Recombinant chicken IL-6 does not activate heterophils isolated from day-old chickens in vitro.

    abstract：:Pro-inflammatory cytokines are produced as part of innate immunity. Increased resistance to extraintestinal Salmonella enteritidis (SE) has been associated with an increase in heterophil pro-inflammatory cytokine gene expression. Invasion of chicken epithelial cells by SE induces an 8- to 10-fold increase in interleuk...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2004.08.002

    authors： Ferro PJ,Swaggerty CL,He H,Rothwell L,Kaiser P,Kogut MH

    更新日期：2005-01-01 00:00:00

  • Molecular characterization and expression analysis of TLR1 and TLR4 from the endangered fish Dabry's sturgeon (Acipenser dabryanus).

    abstract：:Toll-like receptors (TLRs) are important pattern recognition receptors (PRRs) of teleost innate immune system. However, information about TLRs is absent in Dabry's sturgeon (Acipenser dabryanus), one of the most primitive actinopterygii species. In the present study, the full lengths of adaTLR1 and adaTLR4 were cloned...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2018.05.009

    authors： Han P,Wang S,Zhang Q,Zhang S,Shao R,Xu W,Zhang W,Xu Q,Wei Q,Qi Z

    更新日期：2018-09-01 00:00:00

  • Lipopolysaccharide enhances bactericidal activity in Dictyostelium discoideum cells.

    abstract：:Innate immune cells respond to invading microbes upon detection of pathogen-associated molecular patterns (PAMPS). PAMP-recognition machinery is evolutionarily conserved, allowing for characterization in model organisms. The model organism Dictyostelium discoideum can exist as single-celled amoebae, which phagocytize ...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2011.03.018

    authors： Walk A,Callahan J,Srisawangvong P,Leuschner J,Samaroo D,Cassilly D,Snyder ML

    更新日期：2011-08-01 00:00:00

  • Characterization of crustins from the hemocytes of the spider crab, Hyas araneus, and the red king crab, Paralithodes camtschaticus.

    abstract：:Crustins are distributed across the decapods and are believed to play a significant part in the humoral defense system of their host. In this study, two crustin isoforms from Hyas araneus hemocytes were purified and tested for antimicrobial activity against selected microorganisms. They show both antibacterial and ant...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2008.10.010

    authors： Sperstad SV,Haug T,Paulsen V,Rode TM,Strandskog G,Solem ST,Styrvold OB,Stensvåg K

    更新日期：2009-04-01 00:00:00

  • Immune system cell markers in the northern brown bandicoot, Isoodon macrourus.

    abstract：:This is the first study to report the presence of T and B lymphocyte markers and antigen presenting-like molecules in a marsupial bandicoot. Intra-cytoplasmic markers for CD3 and CD5, as well as surface Thy-1.1 and CD1a molecules were located in lymphocytes of T dependent regions of immuno-lymphoid tissue in the north...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/s0145-305x(00)00030-6

    authors： Cisternas PA,Armati PJ

    更新日期：2000-12-01 00:00:00

  • Regulation of FOXP3 expression in myeloid cells in response to all-trans-retinoic acid, interleukin 2 and transforming growth factor β.

    abstract：:FoxP3 is a transcription factor essential for differentiation and function of T regulatory cells (Tregs). There are two major subsets of Tregs: natural Tregs (nTregs) generated in thymus and inducible Tregs (iTregs) produced in peripheral immune system. It has been documented that iTreg development is dependent on sol...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2019.02.019

    authors： Ilnicka A,Gocek E,Łopatecka J,Marcinkowska E

    更新日期：2019-07-01 00:00:00

  • Epitopes for chicken monoclonal antibodies in spleens of selected Japanese quail lines.

    abstract：:A line of Japanese quail selected for high plasma cholesterol is highly susceptible to diet-induced atherosclerosis. Lymphocyte epitopes recognized by mouse anti-chicken monoclonal antibodies (c-mAb), TCR-1, TCR-2, TCR-3. CD-3, CD-4, CD-8, and BU-1a/b were reacted with spleens from quail selected for high (HL) and low...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/s0145-305x(99)00033-6

    authors： Lovitt HL,Siegel HS,Weinstock D,Marks HL

    更新日期：1999-09-01 00:00:00

  • Identification of a novel relish homolog in Chinese shrimp Fenneropenaeus chinensis and its function in regulating the transcription of antimicrobial peptides.

    abstract：:Penaeid shrimp, as an invertebrate, relies on the innate immunity to oppose the microbial invaders. Antimicrobial peptides (AMP) are an integral component of the innate immune system in most organisms and function as an early first line of defense against pathogens, but the knowledge about the pathways to regulate the...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2009.06.001

    authors： Li F,Yan H,Wang D,Priya TA,Li S,Wang B,Zhang J,Xiang J

    更新日期：2009-10-01 00:00:00

  • DNA content and proliferation of rat thymocytes.

    abstract：:Scanning microdensitometric analysis of Feulgen stained thymocytes from rats aged 1 to 71 days revealed the presence of a number of waves of cell proliferation which may correlate with the development of functionally distinct T-cell subpopulations. In addition, considerable numbers of hypodiploid cells were seen. It i...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/0145-305x(84)90021-1

    authors： Rippin JW

    更新日期：1984-01-01 00:00:00

  • Characterization of two novel ADP ribosylation factors from giant freshwater prawn Macrobrachium rosenbergii and their responses to WSSV challenge.

    abstract：:ADP-ribosylation factors (Arfs) are small GTP-binding proteins that have an essential function in intracellular trafficking and organelle structure. To date, little information is available on the Arfs in the economically important giant freshwater prawn Macrobrachium rosenbergii and their relationship to viral infect...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2014.10.006

    authors： Ding ZF,Ren J,Tan JM,Wang Z,Yin SW,Huang Y,Huang X,Wang W,Lan JF,Ren Q

    更新日期：2015-01-01 00:00:00

  • In vivo and in vitro knockdown of FREP2 gene expression in the snail Biomphalaria glabrata using RNA interference.

    abstract：:RNA interference (RNAi) is reported here for the first time for Biomphalaria glabrata, the snail intermediate host for the human parasite Schistosoma mansoni. The fibrinogen-related protein 2 (FREP2) gene, normally expressed at increased levels following exposure to digenetic trematode parasites, such as S. mansoni or...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2005.12.004

    authors： Jiang Y,Loker ES,Zhang SM

    更新日期：2006-01-01 00:00:00

  • Isolation and initial characterisation of complement components C3 and C4 of the nurse shark and the channel catfish.

    abstract：:Complement components C3 and C4 have been isolated from the serum of the nurse shark (Ginglymostoma cirratum) and of the channel catfish (Ictalurus punctatus). As in the higher vertebrates, the fish C4 proteins have three-chain structures while the C3 proteins have two-chain structures. All four proteins have intra-ch...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/s0145-305x(98)00002-0

    authors： Dodds AW,Smith SL,Levine RP,Willis AC

    更新日期：1998-03-01 00:00:00

  • Genetics of anti-parasite resistance in invertebrates.

    abstract：:This review summarizes and compares available data on genetic and molecular aspects of resistance in four well-described invertebrate host-parasite systems: snail-schistosome, mosquito-malaria, mosquito-filarial worm, and Drosophila-wasp associations. It underlies that the major components of the immune reaction, such...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.dci.2004.05.004

    authors： Carton Y,Nappi AJ,Poirie M

    更新日期：2005-01-01 00:00:00

  • Molecular cloning, characterization and function of a germinal center kinase MST4 gene from Litopenaeus vannamei in response to Vibrio alginolyticus challenge in TLR-TRAF6 signaling pathway.

    abstract：:The serine/threonine protein kinase MST4 plays multiple roles in the regulation of signaling pathways that govern cellular processes including mitosis, migration, homeostasis, polarity, proliferation, differentiation and apoptosis. Here we report the identification and characterization of the full-length sequence of L...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2017.03.026

    authors： Zhao CS,Huang D,Peng T,Huang MZ,Xie CY,Chen J,Kong JR,Xie RC,Liu Y,Wang WN

    更新日期：2017-08-01 00:00:00

  • Surface immunoglobulin on granular and agranular leukocytes in the thymus and spleen of the snapping turtle, Chelydra serpentina.

    abstract：:Spleen and thymus suspensions from the turtle, Chelydra serpentina were examined by indirect immunofluorescence and found to contain both agranulocytes and granulocytes positive for surface Ig. Among the splenic agranulocytes , 50% of the lymphocyte and monocyte population was positive for surface Ig. In the thymus on...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/0145-305x(84)90015-6

    authors： Mead KF,Borysenko M

    更新日期：1984-01-01 00:00:00

  • Function of CCL5 in maternal-fetal interface of pig during early pregnancy.

    abstract：:Chemokines refer to chemoattractant cytokines, which have crucial functions in inflammation and immune responses in multiple cellular processes. In the present study, we described the potential role of porcine CCL5 in embryo implantation and fetal-maternal environment during early pregnancy. We first carried out phylo...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2019.103503

    authors： Bae H,Lee JY,Song G,Lim W

    更新日期：2020-02-01 00:00:00