Functional elucidation of LvToll 3 receptor from P. vannamei through RNA interference and its potential role in the shrimp antiviral response.

Abstract:

:There is a continuing debate on whether an antiviral immunity similar to vertebrate interferon response exists in invertebrates. Recent advances in penaeid immunology identified several new members of the Toll receptor family and one of these is LvToll3 (Litopenaeus vannamei Toll3). It is hypothesized in this study that LvToll3 responds to pathogen associated molecular patterns (PAMPs) such as dsRNA, which then activates certain antiviral pathways in penaeids. RNA interference (RNAi) was used to determine differences in the expression levels of specific genes putatively involved in the antiviral response through qPCR. Results showed that LvToll3 upregulation could be elicited through the introduction of double stranded RNA (dsRNA) regardless of sequence relative to initial levels in the 3rd hour. Furthermore, statistically intriguing trend in the overall expression of Vago 4/5 and Interferon regulatory factor (IRF) suggests that both these genes are affected by the expression of LvToll3. Dicer showed no statistical difference between the experimentally treated (LvToll3-dsRNA), positive control (GFP-dsRNA), and control (PBS) samples corroborating the assertion that dicer is part of another antiviral mechanism that acts in concert with Toll system. These findings suggests that LvToll3 plays a critical role in penaeid antiviral immunity when molecular patterns associated with viruses are detected.

journal_name

Dev Comp Immunol

authors

Guanzon DAV,Maningas MBB

doi

10.1016/j.dci.2018.01.020

subject

Has Abstract

pub_date

2018-07-01 00:00:00

pages

172-180

eissn

0145-305X

issn

1879-0089

pii

S0145-305X(17)30608-0

journal_volume

84

pub_type

杂志文章
  • The genomic structure, alternative splicing and immune response of Chlamys farreri thioester-containing protein.

    abstract::CfTEP is a member of thioester-containing protein (TEP) family found in Zhikong scallop Chlamys farreri and is involved in innate immunity against invading microbes. In the present study, the genomic DNA of CfTEP was cloned and characterized. The genomic DNA sequence of CfTEP consisted of 40 exons and 39 introns spann...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2009.05.007

    authors: Zhang H,Wang L,Song L,Zhao J,Qiu L,Gao Y,Song X,Li L,Zhang Y,Zhang L

    更新日期:2009-10-01 00:00:00

  • The diversity of C-type lectins in the genome of a basal metazoan, Nematostella vectensis.

    abstract::C-type lectins (CTLs) are involved in cell-cell adhesion, recognition, and innate immunity in higher vertebrates, but little is known about CTLs in basal metazoans. The recent sequencing of the cnidarian Nematostella vectensis genome allowed us to explore the CTL-like gene family at the base of metazoan evolution. Six...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2009.01.008

    authors: Wood-Charlson EM,Weis VM

    更新日期:2009-08-01 00:00:00

  • In vitro interaction of Perkinsus marinus merozoites with eastern and Pacific oyster hemocytes.

    abstract::This study compared hemocyte responses of eastern and Pacific oysters to Perkinsus marinus, in vitro. Except for the percentage of hemocytes associated with P. marinus there was little or no significant difference between eastern and Pacific oysters with regard to their hemocytic response to P. marinus. In phagocytosi...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/0145-305x(95)00017-n

    authors: La Peyre JF,Chu FL,Vogelbein WK

    更新日期:1995-07-01 00:00:00

  • A novel C-type lectin (FcLec4) facilitates the clearance of Vibrio anguillarum in vivo in Chinese white shrimp.

    abstract::C-type lectins play important roles in innate immunity of invertebrates. In the present study, we report a novel C-type lectin, named FcLec4, from the Chinese white shrimp Fenneropenaeus chinensis. FcLec4 contains a single carbohydrate recognition domain (CRD) with a putative signal peptide. Phylogenetic analysis indi...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2009.05.004

    authors: Wang XW,Zhang XW,Xu WT,Zhao XF,Wang JX

    更新日期:2009-09-01 00:00:00

  • Host defense peptides in skin secretions of the Oregon spotted frog Rana pretiosa: implications for species resistance to chytridiomycosis.

    abstract::Population declines due to chytridiomycosis among frogs belonging to the Amerana (Rana boylii) species group from western North America have been particularly severe. Norepinephrine-stimulated skin secretions from the Oregon spotted frog Rana pretiosa Baird and Girard, 1853 were collected from individuals that had bee...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2011.01.017

    authors: Conlon JM,Mechkarska M,Ahmed E,Coquet L,Jouenne T,Leprince J,Vaudry H,Hayes MP,Padgett-Flohr G

    更新日期:2011-06-01 00:00:00

  • Characterization of crustins from the hemocytes of the spider crab, Hyas araneus, and the red king crab, Paralithodes camtschaticus.

    abstract::Crustins are distributed across the decapods and are believed to play a significant part in the humoral defense system of their host. In this study, two crustin isoforms from Hyas araneus hemocytes were purified and tested for antimicrobial activity against selected microorganisms. They show both antibacterial and ant...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2008.10.010

    authors: Sperstad SV,Haug T,Paulsen V,Rode TM,Strandskog G,Solem ST,Styrvold OB,Stensvåg K

    更新日期:2009-04-01 00:00:00

  • The effect of aging on T cell responses in the horse.

    abstract::Horses greater than 20 years of age exhibit alterations in their immune responses similar to those observed in other aged individuals. The purpose of this study was to characterize immunosenescence in a population of aged ponies. The peripheral blood mononuclear cells (PBMC) from aged ponies exhibited a decreased prol...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/s0145-305x(01)00027-1

    authors: Horohov DW,Kydd JH,Hannant D

    更新日期:2002-01-01 00:00:00

  • Recent advances on the immunobiology of Bithynia spp. hosts of Opisthorchis viverrini.

    abstract::This article reviews the past and present scientific reports regarding Bithynia spp. focusing on the biology, ecology and life cycle of Bithynia snails and their responses to Opisthorchis viverrini infection. Moreover, new data regarding comparative molecular genomics and proteomic approaches have recently revealed no...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章,评审

    doi:10.1016/j.dci.2019.103460

    authors: Suwannatrai K,Suwannatrai AT,Loukas A,Sotillo J

    更新日期:2020-01-01 00:00:00

  • Isolation and characterization of a human alternative complement pathway-inhibiting protein from larval hemolymph of the silkworm, Bombyx mori.

    abstract::An alternative complement pathway-inhibiting protein (ACPIP), which inhibits the activation of the alternative complement pathway (ACP) of the human serum, was isolated from larval hemolymph of the silkworm, Bombyx mori, by using ammonium sulfate fractionation and column chromatographies to homogeneity. About 400micro...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/s0145-305x(00)00046-x

    authors: Sekijima Y,Fujikura Y,Hiyama T,Maenaka T,Uda A

    更新日期:2001-03-01 00:00:00

  • Differential expression and functional diversification of diverse immunoglobulin domain-containing protein (DICP) family in three gynogenetic clones of gibel carp.

    abstract::Diverse immunoglobulin (Ig) domain-containing protein (DICP) family is a novel bony fish-specific multi-gene family encoding diversified immune receptors. However, their function and the implication of binding partners remain unknown. In this study, we first identified 28 DICPs from three gibel carp gynogenetic clones...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2018.03.013

    authors: Gao FX,Lu WJ,Wang Y,Zhang QY,Zhang YB,Mou CY,Li Z,Zhang XJ,Liu CW,Zhou L,Gui JF

    更新日期:2018-07-01 00:00:00

  • Expression, signal transduction, and function analysis of TIRAP and TRIF in Nile tilapia (Oreochromis niloticus).

    abstract::Toll/interleukin 1 receptor domain-containing adaptor protein (TIRAP) and toll/interleukin 1 receptor-domain-containing adapter-inducing interferon-β (TRIF) are crucial adaptors of signal transduction for the signaling pathways of toll-like receptors (TLRs). TIRAP and TRIF perform an essential function in an antimicro...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2020.103991

    authors: Trung NB,Nan FH,Wang IJ,Wu YC,Wen CM,Lee MC,Hang HT,Lee PT

    更新日期:2021-04-01 00:00:00

  • TNF induces the growth of thymocytes in rainbow trout.

    abstract::In order to investigate the effects of TNFalpha upon the growth of fish thymocytes, rainbow trout thymocytes were cultured in the conditioned medium (CM): the supernatants of the macrophage cultures stimulated with chitin derivative and LPS. Synthesis of TNFalpha by macrophages and subsequent secretion into CM were as...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2005.09.005

    authors: Hino K,Nakamura O,Yoshiura Y,Suetake H,Suzuki Y,Watanabe T

    更新日期:2006-01-01 00:00:00

  • A DM9-containing protein from oyster Crassostrea gigas (CgDM9CP-3) mediating immune recognition and encapsulation.

    abstract::DM9 domain containing protein (DM9CP) is a recently identified pattern recognition molecules exiting in most organisms except plants. In the present study, a novel DM9-containing protein (CgDM9CP-3) was identified from Pacific oyster Crassostrea gigas with an open reading frame of 438 bp, encoding a polypeptide of 145...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2020.103937

    authors: Liu Y,Wang W,Zhao Q,Yuan P,Li J,Song X,Liu Z,Ding D,Wang L,Song L

    更新日期:2021-03-01 00:00:00

  • IL-12 and IL-18 induce interferon-γ production and de novo CD2 expression in porcine γδ T cells.

    abstract::γδ T cells are highly abundant in the blood and spleen of pigs but little is known about their functional differentiation. In this study the potential of the type-1 polarizing cytokines IL-12 and IL-18 in combination with IL-2 and Concanavalin A (ConA) to stimulate porcine γδ T cells was investigated. Stimulation of p...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2014.07.007

    authors: Sedlak C,Patzl M,Saalmüller A,Gerner W

    更新日期:2014-11-01 00:00:00

  • Purification and properties of agglutinins from conger eel, Conger Myriaster (Brevoort), skin mucus.

    abstract::The skin mucus of the conger eel, Conger myriaster, contains galactose-specific agglutinins. Hemagglutinating activity is independent of divalent cations and is destroyed by heating at 50 degrees C for 15 min. The mucus agglutinins, named congerins, are a mixture of proteins with different electrical charges. Three of...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/0145-305x(88)90007-9

    authors: Kamiya H,Muramoto K,Goto R

    更新日期:1988-04-01 00:00:00

  • Age-related suppression of murine lymphoid cell blastogenesis by marijuana components.

    abstract::Marijuana components modulate a variety of immune response parameters. The cannabinoids delta 9-tetrahydrocannabinol (THC) and 11-hydroxy-tetrahydrocannabinol (11 OH-THC) are known to depress the in vitro proliferative response of murine lymphoid cells to the mitogens concanavalin A (Con A) and phytohemagglutinin (PHA...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/0145-305x(90)90015-7

    authors: Pross SH,Klein TW,Newton C,Smith J,Widen R,Friedman H

    更新日期:1990-01-01 00:00:00

  • Transcriptome analysis of hagfish leukocytes: a framework for understanding the immune system of jawless fishes.

    abstract::Jawless fishes occupy a critical phylogenetic position in understanding the origin of the adaptive immune system. Here, we performed large-scale expressed sequence tag analysis of leukocytes isolated from the inshore hagfish Eptatretus burgeri. Although we found many immunity-related genes such as those involved in ly...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2004.04.005

    authors: Suzuki T,Shin-I T,Kohara Y,Kasahara M

    更新日期:2004-08-01 00:00:00

  • O-antigen-deficient, live, attenuated Salmonella typhimurium confers efficient uptake, reduced cytotoxicity, and rapid clearance in chicken macrophages and lymphoid organs and induces significantly high protective immune responses that protect chickens ag

    abstract::In the present study, we developed an O-antigen-deficient, live, attenuated Salmonella Typhimurium (ST) strain (JOL2377) and assessed its safety, macrophage toxicity, invasion into lymphoid tissues, immunogenicity, and protection against Salmonella infection in chickens. The JOL2377 induced significantly lower cytotox...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2020.103745

    authors: Senevirathne A,Hewawaduge C,Park S,Park JY,Kirthika P,Lee JH

    更新日期:2020-10-01 00:00:00

  • Epitopes for chicken monoclonal antibodies in spleens of selected Japanese quail lines.

    abstract::A line of Japanese quail selected for high plasma cholesterol is highly susceptible to diet-induced atherosclerosis. Lymphocyte epitopes recognized by mouse anti-chicken monoclonal antibodies (c-mAb), TCR-1, TCR-2, TCR-3. CD-3, CD-4, CD-8, and BU-1a/b were reacted with spleens from quail selected for high (HL) and low...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/s0145-305x(99)00033-6

    authors: Lovitt HL,Siegel HS,Weinstock D,Marks HL

    更新日期:1999-09-01 00:00:00

  • Evolution of low molecular weight immunoglobulins. V. Degree of antigenic relationship between the 7S immunoglobulins of mammals, birds, and lower vertebrates to the turkey IgY.

    abstract::There are close antigenic relations between the turkey IgY--the dominant 7S Ig of serum--and the corresponding IgY-like Igs of chicken, duck, goose, tortoise, and frog as well as serum or colostral IgA of man and swine. The RIA-systems used for the determination of immunological cross-reactivity among the Ig types con...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/0145-305x(86)90027-3

    authors: Hädge D,Ambrosius H

    更新日期:1986-07-01 00:00:00

  • Immulectin-2, a pattern recognition receptor that stimulates hemocyte encapsulation and melanization in the tobacco hornworm, Manduca sexta.

    abstract::In insects, encapsulation followed by melanization is a major defense mechanism against metazoan parasites. However, insects must recognize and differentiate nonself before they mount an immune response. Recognition of pathogens in insects is accomplished by a set of pattern recognition receptors (PRRs). Binding of PR...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2004.02.005

    authors: Yu XQ,Kanost MR

    更新日期:2004-07-01 00:00:00

  • Phenotypic and functional characterization of earthworm coelomocyte subsets: Linking light scatter-based cell typing and imaging of the sorted populations.

    abstract::Flow cytometry is a common approach to study invertebrate immune cells including earthworm coelomocytes. However, the link between light-scatter- and microscopy-based phenotyping remains obscured. Here we show, by means of light scatter-based cell sorting, both subpopulations (amoebocytes and eleocytes) can be physica...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2016.06.017

    authors: Engelmann P,Hayashi Y,Bodó K,Ernszt D,Somogyi I,Steib A,Orbán J,Pollák E,Nyitrai M,Németh P,Molnár L

    更新日期:2016-12-01 00:00:00

  • The age-dependent size distribution of chicken peripheral blood cells analyzed by a novel method utilizing a Coulter counter.

    abstract::In a dilute solution of Tris-buffered ammonium chloride (TAC), chicken erythrocytes (RBC) swell but chicken white blood cells (WBC) do not. This allowed us to separate the peaks of RBC and wBC into widely separate channels using a Coulter Counter interfaced to a microcomputer to provide size distributions. Analyses of...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/0145-305x(84)90044-2

    authors: Kai O,Franklin RM

    更新日期:1984-04-01 00:00:00

  • Encapsulation ability of Drosophila melanogaster: a genetic analysis.

    abstract::Insects are able to effectively recognize parasitoid eggs or larvae and to eliminate them by formation of a hemocytic capsule. Although the cellular process is now well documented, the genetic aspects of recognition of foreignness and the encapsulation process are still poorly understood. Experiments using the isofema...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/0145-305x(85)90112-0

    authors: Carton Y,Boulétreau M

    更新日期:1985-04-01 00:00:00

  • Characterization of serum immunoglobulins in a chondrostean fish, Acipenser baeri.

    abstract::The euglobulin fraction of sturgeon (Acipenser baeri) serum was analyzed using electrophoretic and immunoblotting techniques. The major protein of this fraction is an IgM-like molecule composed of equimolar 70-kDa glycosylated H chains and 26-30 kDa L chains. In the absence of a reducing agent, the L and H polypeptide...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/s0145-305x(05)80007-2

    authors: Partula S,Charlemagne J

    更新日期:1993-11-01 00:00:00

  • Spatial and temporal transcriptomic analyses reveal locust initiation of immune responses to Metarhizium acridum at the pre-penetration stage.

    abstract::Insect hemocyte and fat body tissues play critical functional roles in insect immunity. Little, however, is known concerning the dynamic responses of these tissues to fungal infection. Here, we report on a time course of locust hemocyte and fat body transcriptomic responses to infection by the acridid specific fungal ...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2019.103524

    authors: Zhang W,Zheng X,Chen J,Keyhani NO,Cai K,Xia Y

    更新日期:2020-03-01 00:00:00

  • Molecular cloning and characterization of autophagy-related gene TmATG8 in Listeria-invaded hemocytes of Tenebrio molitor.

    abstract::Macroautophagy (hereinafter called autophagy) is a highly regulated process used by eukaryotic cells to digest portions of the cytoplasm that remodels and recycles nutrients and disposes of unwanted cytoplasmic constituents. Currently 36 autophagy-related genes (ATG) and their homologs have been characterized in yeast...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2015.02.017

    authors: Tindwa H,Jo YH,Patnaik BB,Lee YS,Kang SS,Han YS

    更新日期:2015-07-01 00:00:00

  • Genetics of anti-parasite resistance in invertebrates.

    abstract::This review summarizes and compares available data on genetic and molecular aspects of resistance in four well-described invertebrate host-parasite systems: snail-schistosome, mosquito-malaria, mosquito-filarial worm, and Drosophila-wasp associations. It underlies that the major components of the immune reaction, such...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章,评审

    doi:10.1016/j.dci.2004.05.004

    authors: Carton Y,Nappi AJ,Poirie M

    更新日期:2005-01-01 00:00:00

  • The M2 macrophages induce autophagic vascular disorder and promote mouse sensitivity to urethane-related lung carcinogenesis.

    abstract::Tumor vessels are known to be abnormal, with typically aberrant, leaky and disordered vessels. Here, we investigated whether polarized macrophage phenotypes are involved in tumor abnormal angiogenesis and what is its mechanism. We found that there was no difference in chemotaxis of polarized M1 and M2 macrophages to l...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/j.dci.2016.01.010

    authors: Li GG,Guo ZZ,Ma XF,Cao N,Geng SN,Zheng YQ,Meng MJ,Lin HH,Han G,Du GJ

    更新日期:2016-06-01 00:00:00

  • Phylogeny of lymphocyte heterogeneity. I. Membrane immunoglobulins of teleost lymphocytes.

    abstract::Immunofluorescence studies of bream lymphoid tissues revealed that our 90% of the lymphocytes from blood, anterior kidney, spleen and thymus exhibited membrane immunoglobulin determinants. Furthermore, a majority of such cells were observed to undergo patching and capping when the membrane proteins were complexed with...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章

    doi:10.1016/s0145-305x(77)80004-9

    authors: Clem LW,McLean WE,Shankey VT,Cuchens MA

    更新日期:1977-04-01 00:00:00