Abstract:
:We report on the cloning and characterization of Trypanosoma carassii 70 KDa heat shock protein (hsp70). T. carassii hsp70 was secreted/excreted into culture medium in vitro and was recognized by sera from infected fish. Recombinant hsp70 (rhsp70) activated goldfish macrophages and stimulated the production of pro-inflammatory cytokines including interferon gamma (IFNgamma), tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, (IL)-12 and chemokines CCL-1 and CXCL-8 (IL-8). T. carassii hsp70-induced cytokine expression was abrogated by pronase treatment of macrophages confirming the existence of receptor(s) on goldfish macrophage surface that recognize parasite molecule. Parasite hsp70 also up-regulated the expression inducible nitric oxide synthase (iNOS) isoforms A and B and induced a strong nitric oxide response of goldfish macrophages.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Oladiran A,Belosevic Mdoi
10.1016/j.dci.2009.06.003subject
Has Abstractpub_date
2009-10-01 00:00:00pages
1128-36issue
10eissn
0145-305Xissn
1879-0089pii
S0145-305X(09)00127-Xjournal_volume
33pub_type
杂志文章abstract::Differences in responses of chicken bone marrow derived dendritic cells (BMDC) to in vitro treatment with lipopolysaccharide (LPS), heat, and LPS + heat were identified. The Fayoumi is more disease resistant and heat tolerant than the Leghorn line. Nitric Oxide (NO) production, phagocytic ability, MHC II surface expre...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2016.05.016
更新日期:2016-10-01 00:00:00
abstract::The ingestion of sheep erythrocytes (SRBC) by echinoid phagocytes was greatly increased after treatment of SRBC with sera from mouse, human and fish. The opsonic principle in the sera was heat labile (56 degrees C 1/2 hr). Opsonization with mammalian sera was studied more extensively. It was dependent on pre-sensitiza...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/s0145-305x(82)80028-1
更新日期:1982-07-01 00:00:00
abstract::Heterogeneous nuclear ribonucleoproteins (hnRNPs) are highly conserved from nematode to mammal because they play an important role in several aspects of pre-mRNA maturation, including RNA packaging and alternative splicing. In Drosophila, the hnRNP A1 homolog hrp36 regulates alternative splicing in several genes, incl...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2011.05.006
更新日期:2012-01-01 00:00:00
abstract::Siglec-3/CD33 is a myeloid-specific inhibitory receptor that is expressed on cells of the immune system, where it is believed to play a regulatory role, modulating the inflammatory and immune responses. We characterized CD33 (RbCD33) in rock bream which is a transmembrane protein with two IG-like domains and a cytopla...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2018.02.005
更新日期:2018-07-01 00:00:00
abstract::This article has been withdrawn at the request of the author(s). The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2012.05.002
更新日期:2012-05-15 00:00:00
abstract::Crustins are distributed across the decapods and are believed to play a significant part in the humoral defense system of their host. In this study, two crustin isoforms from Hyas araneus hemocytes were purified and tested for antimicrobial activity against selected microorganisms. They show both antibacterial and ant...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2008.10.010
更新日期:2009-04-01 00:00:00
abstract::The identification of molecular markers considerably facilitated the classification and functional analysis of blood cell types. Apis mellifera hemocytes have been classified by morphological criteria and lectin binding properties; however, the use of molecular markers has been minimal. Here we describe a monoclonal a...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2017.07.013
更新日期:2017-11-01 00:00:00
abstract::Mammalian CCL20, or macrophage inflammatory protein-3α, can function as a homeostatic and inflammatory chemokine. In relation to the latter, it is responsible for the chemoattraction of lymphocytes and dendritic cells to mucosal immune sites under inflammatory and pathological conditions. CK1, CK8A and CK8B are rainbo...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2019.103502
更新日期:2020-02-01 00:00:00
abstract::Manganese superoxide dismutases (MnSODs) are usually mitochondrial enzymes, although there are few examples of cytosolic MnSODs (cMnSOD). We have previously characterized a cMnSOD cDNA from Litopenaeus vannamei hemocytes, and to obtain new insights into the tissue specific expression and the protein structure, we char...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2006.11.007
更新日期:2007-01-01 00:00:00
abstract::As physical barriers that separate teleost fish from the external environment, mucosae are also active immunological sites that protect them against exposure to microbes and stressors. In mammals, the sites where antigens are sampled from mucosal surfaces and where stimulation of naïve T and B lymphocytes occurs are k...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章,评审
doi:10.1016/j.dci.2011.11.009
更新日期:2011-12-01 00:00:00
abstract::The phagocytic hemocytes of four snail species were investigated for their ability to generate reactive oxygen metabolites upon stimulation by foreign material. Hemocytes of the pond snail Lymnaea stagnalis and of the garden snail Helix aspersa showed a luminol-dependent chemiluminescence (CL) when they phagocytosed z...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(88)90068-7
更新日期:1988-07-01 00:00:00
abstract::Anti-lipopolysaccharide factor (ALF) is an antimicrobial peptide originally identified from horseshoe crabs and recently found in several shrimp species. ALFPm3, the most abundant isoform in the black tiger shrimp, Penaeus monodon, has been shown to possess a broad spectrum of antimicrobial activity against Gram-negat...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2008.03.008
更新日期:2008-01-01 00:00:00
abstract::The purpose of this study was to investigate in rats, by double-label immunofluorescence and flow cytometric analysis, the age related changes in the CD4 subset of gut-associated lymphoid tissues and spleen. We found that the percentage of CD4+ T cells in Peyer's patches (PP) and spleen (SP) increased during the first...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/s0145-305x(97)00023-2
更新日期:1997-09-01 00:00:00
abstract::Two isoforms of plasmolipin were initially identified from the black tiger shrimp (Penaeus monodon) EST database and completed using 50 RACE to reveal complete cDNAs of 558 bp (PmPLP1) and 537 bp(PmPLP2) with 87% nucleotide sequence identity. The deduced amino acid sequences contained four-transmembrane domains and sh...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2012.06.009
更新日期:2012-10-01 00:00:00
abstract::Monoclonal antibodies (mAbs) specific for a putative antigen receptor (function associated molecule, FAM) on fish NCC were tested for their ability to initiate signal transduction responses. Anti-FAM hybridoma target cells were significantly lysed by NCC when tested by redirected lysis (RL). Redirected lysis was enhan...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(92)90040-j
更新日期:1992-09-01 00:00:00
abstract::Serine protease inhibitors (SPIs) play a key role in physiological responses by controlling protease activities. In this study, we studied the biochemical functions of serpin-15, an SPI, from Bombyx mori (Bmserpin-15). Recombinant Bmserpin-15 was expressed in Escherichia coli cells and used to raise rabbit anti-Bmserp...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2015.02.013
更新日期:2015-07-01 00:00:00
abstract::Toll-like receptors (TLRs) play an important role in the activation of innate and adaptive immune responses. Stimulation with multiple TLR agonists may result in synergistic, complimentary or inhibitory effects on innate immune responses. In this study, we investigated the effects of co-stimulation of sheep peripheral...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2009.12.018
更新日期:2010-05-01 00:00:00
abstract::The chemokine RANTES plays an essential role in inflammation and immune response. In this study, we cloned the nucleotide sequence of the 5'-flanking region of the porcine RANTES (poRANTES) gene and characterized the regulatory elements that activate transcription. Analyses of a series of 5' deletion constructs demons...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2010.10.008
更新日期:2011-03-01 00:00:00
abstract::A range of marine invertebrates were screened for prophenoloxidase (a marker protein of the prophenoloxidase activating system) in the coelomic fluid or hemolymph. The crustaceans and the ascidian, Ciona intestinalis, displayed strongest activity, with low levels found in the starfish, Asterias rubens, the sea urchin,...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(91)90018-t
更新日期:1991-10-01 00:00:00
abstract::Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that caused severe egg drop syndrome in laying ducks in China since 2010, leading to massive economic losses to the duck industry. Although the DTMUV E protein is considered to be critical in inducing the protective immune response, the functional ep...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2020.103906
更新日期:2021-02-01 00:00:00
abstract::Adult Xenopus laevis serum containing an anti-chicken erythrocyte antibody exhibits complement (C) activity by both the classical and the alternative pathways. Lytic activity is removed by heating serum to 50 degrees C for at least 20 min; it is also removed by polysaccharides and by 7.5 mM EDTA. The optimum temperatu...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(87)90047-4
更新日期:1987-07-01 00:00:00
abstract::In Xenopus, the type I transmembrane protein, CTX (cortical thymocyte-specific antigen of Xenopus), is hypothesized to play a role in thymocyte differentiation and/or thymic selection. The present studies were undertaken to gather additional evidence in support of a postulated association of CTX with a differentiation...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/s0145-305x(98)00028-7
更新日期:1998-09-01 00:00:00
abstract::Molecular phylogenetics and its applications are popular and useful tools for making comparative investigations in genetics; however, estimating phylogenetic trees is not always straightforward. Some phylogenetic estimators use an explicit model of nucleotide evolution to estimate evolutionary parameters such as branc...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章,评审
doi:10.1016/j.dci.2004.07.007
更新日期:2005-01-01 00:00:00
abstract::Neonates of all species, including foals, are highly susceptible to infection, and neutrophils play a crucial role in innate immunity to infection. Evidence exists that neutrophils of neonatal foals are functionally deficient during the first weeks of life, including expression of cytokine genes such as IFNG. We hypot...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2018.06.012
更新日期:2018-10-01 00:00:00
abstract::To date, many immunoglobulin (Ig) genes have been identified in diverse teleost species, but the contributions of different types of light chain (IgL) to the immune response remain unclear. Screening of a stimulated kidney cDNA library from orange-spotted grouper (Osg, Epinephelus coioides) resulted in the identificat...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2012.11.003
更新日期:2013-03-01 00:00:00
abstract::In order to assess the role of antigen persistence in the tolerant state, tolerance was induced in Xenopus laevis by the embryonic transplantation of whole eyes or tail tissue. Both types of transplants were seen to heal in and persist, with no signs of immunological incompatibility. At metamorphosis, tail resorption ...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(83)90034-4
更新日期:1983-07-01 00:00:00
abstract::Intestinal mucosal immune system development was investigated in fetal lambs from 61 to 110 days (term, 150 days). Fetal small intestine was examined at 400 mm intervals for the presence of IgM(+) cells. The first phase of B cell development was characterised by increase in B cell density throughout the jejunum from 6...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/s0145-305x(02)00139-8
更新日期:2003-06-01 00:00:00
abstract::Two clones, designated Icpu-UA/3 and Icpu-UA/26, were isolated from a genomic library prepared from a single homozygous gynogenetic channel catfish. Sequence analysis showed that each clone encoded a gene product containing features conserved among MHC class I molecules. The genomic organization of both clones indicat...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/s0145-305x(01)00017-9
更新日期:2001-09-01 00:00:00
abstract::In the present study, we developed an O-antigen-deficient, live, attenuated Salmonella Typhimurium (ST) strain (JOL2377) and assessed its safety, macrophage toxicity, invasion into lymphoid tissues, immunogenicity, and protection against Salmonella infection in chickens. The JOL2377 induced significantly lower cytotox...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2020.103745
更新日期:2020-10-01 00:00:00
abstract::Three molecular forms of immunoglobulins: pentamer, dimer and monomer, were isolated from serum of the frill shark, Chlamydoselachus anguineus, the most primitive extant shark. A pentamer having an apparent mass of 900 kDa, consisting of 68 kDa heavy (H) chains and 22-24 kDa light (L) chains, was considered to be IgM ...
journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(92)90004-v
更新日期:1992-07-01 00:00:00