Ouabain enhances basal and stimulus-induced cytoplasmic calcium concentrations in platelets.

Abstract:

:Incubation of platelet-rich plasma (PRP) with ouabain, an inhibitor of sodium/potassium ATPase (Na+/K+ ATPase), induced a significant rise in basal platelet intracellular calcium concentration [( Ca2+]i) when measured using fura 2. Ouabain induced an enhanced aggregation response to low doses of collagen in both PRP and washed platelets loaded with aequorin. In aequorin loaded platelets this enhanced aggregation response was associated with an enhanced rise in [Ca2+]i such that the relationship between [Ca2+]i and aggregation was unchanged. As inhibition of plasma membrane Na+/K+ ATPase would lead to a raised intracellular sodium ion concentration [( Na+]i) the results suggest that in the platelet, [Na+]i can modulate [Ca2+]i and hence influence the response of platelets to stimuli such as collagen.

journal_name

Thromb Haemost

authors

Lees AD,Wilson J,Orchard CH,Orchard MA

subject

Has Abstract

pub_date

1989-11-24 00:00:00

pages

1000-5

issue

3

eissn

0340-6245

issn

2567-689X

journal_volume

62

pub_type

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