Abstract:
:Activated protein C (APC) is a serine proteinase that regulates blood coagulation. In plasma it is inhibited mainly by the protein C inhibitor (PCI). The plasma concentrations of APC-PCI complex is increased in hypercoagulative states such as deep venous thrombosis. Formation of the APC-PCI complex induces a drastic conformational change in PCI that exposes new epitopes (neoepitopes) on the molecule. We have devised a simple immunofluorometric sandwich assay for measurements of the concentrations of APC-PCI complex, employing as the catcher, a monoclonal antibody that has a high affinity (K(D) = 4 x 10(-11) M) for a complexation-specific neoepitope that is expressed on PCI. A monoclonal antibody against protein C is employed as the tracer. The method gives a linear dose-response curve (0.06-50 microg/l), has a low detection limit (0.06 microg/l) and no crossreactivity with native PCI at physiologic plasma concentrations. We have now determined the concentration of the APC-PCI complex in healthy individuals.
journal_name
Thromb Haemostjournal_title
Thrombosis and haemostasisauthors
Strandberg K,Kjellberg M,Knebel R,Lilja H,Stenflo Jkeywords:
subject
Has Abstractpub_date
2001-08-01 00:00:00pages
604-10issue
2eissn
0340-6245issn
2567-689Xpii
01080604journal_volume
86pub_type
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doi:
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pub_type: 临床试验,杂志文章,随机对照试验
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