当前位置： SCI文献检索 > THROMBOSIS AND HAEMOSTASIS期刊下所有文献 > Discrepancy between tissue factor activity and tissue factor expression in endotoxin-induced monocytes is associated with apoptosis and necrosis.

Discrepancy between tissue factor activity and tissue factor expression in endotoxin-induced monocytes is associated with apoptosis and necrosis.

Abstract：

:Tissue factor (TF), the main initiator of blood coagulation, contributes to the manifestation of disseminated intravascular coagulation following septic shock in meningococcal infection. Since a direct relationship between disease severity and lipopolysaccharide (LPS) concentration in the circulation has been shown, we hypothesized that the procoagulant and cytotoxic effects of endotoxin also in vitro were related to its concentration. In vitro studies, however, have frequently used much higher LPS concentrations than those observed in clinical samples. Using elutriation-purified human monocytes, we observed that LPS up to 1000 ng/ml exerted a concentration-dependent increase in TF activity (tenase activity, fibrin formation in plasma). Although there was a dose-dependent increase in TF activity, there was not a concomitant increase in TF expression at LPS concentrations above 1 ng/ml (flow cytometry, Western blotting, TF mRNA). Flow cytometry revealed that this discrepancy between TF activity and TF expression at endotoxin concentrations above 1 ng/ml, coincided with an LPS dose-dependent increase in cell surface phosphatidylserine (PS), considered to promote coagulation. The increased PS expression was associated with an increased number of 7-AAD-positive cells indicating cell death. We conclude that enhancement of monocyte procoagulant activity in vitro by high concentrations of LPS may result from increased PS exposure due to apoptosis and necrosis. Therefore, the LPS concentrations used to examine monocyte procoagulant activity in vitro, should be carefully chosen.

journal_name

Thromb Haemost

journal_title

Thrombosis and haemostasis

authors

Henriksson CE,Klingenberg O,Ovstebø R,Joø GB,Westvik AB,Kierulf P

doi

10.1160/TH05-07-0463

keywords:

subject

Has Abstract

pub_date

2005-12-01 00:00:00

pages

1236-44

issue

6

eissn

0340-6245

issn

2567-689X

pii

05121236

journal_volume

94

pub_type

杂志文章

相关文献

THROMBOSIS AND HAEMOSTASIS文献大全
  • von Willebrand factor release and P-selectin expression is stimulated by thrombin and trypsin but not IL-1 in cultured human endothelial cells.

    abstract：:The stimulated release of von Willebrand factor (vWF) from endothelial cells by secretagogues such as thrombin is associated with the translocation of Weibel-Palade bodies to the cell membrane and the surface expression of P-selectin (also known as GMP 140, PADGEM and CD 62). P-selectin, which is stored in Weibel-Pala...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Collins PW,Macey MG,Cahill MR,Newland AC

    更新日期：1993-08-02 00:00:00

  • A comparative multi-laboratory assessment of three factor VIII/von Willebrand factor concentrates.

    abstract：:Five expert laboratories have participated in a cross-laboratory study to co-evaluate and compare three commercial Factor VIII/von Willebrand factor (VWF) concentrates. A total of nine factor concentrate lots were evaluated, comprising AHF (High Purity) (AHF HP; x3), Biostate (x3) and Humate/Haemate (x3). All laborato...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Favaloro EJ,Bukuya M,Martinelli T,Tzouroutis J,Duncan E,Welldon K,Collecutt M,Aumann H,Thom J,Gilmore G

    更新日期：2002-03-01 00:00:00

  • Antithrombotic therapy of acute stroke: thrombolytic agents.

    abstract：:Following the initial report of the clinical use of plasminogen activators (PAs) in cerebrovascular thrombosis in 1958, interest in the efficacy of this approach accelerated only when the need for acute intervention in stroke was recognized. The use of PAs in acute ischemic stroke is based on the observation that appr...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章,评审

    doi：

    authors： del Zoppo GJ

    更新日期：1997-07-01 00:00:00

  • Shear stress regulates inflammatory and thrombogenic gene transcripts in cultured human endothelial progenitor cells.

    abstract：:Shear stress has an established effect on mature endothelial cells, but less is known about how shear stress regulates endothelial progenitor cells (EPCs). In vitro expanded EPCs isolated from adult human blood represent a novel tool in regenerative vessel therapy. However, in vitro culturing may generate cells with u...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：10.1160/TH09-12-0854

    authors： Lund T,Hermansen SE,Andreasen TV,Olsen JO,Østerud B,Myrmel T,Ytrehus K

    更新日期：2010-09-01 00:00:00

  • Low Affinity Platelet Factor 4 and High Affinity Platelet Factor 4 - Two Antiheparin Factors Secreted by Human Platelets.

    abstract：: ...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：10.1055/s-0038-1657075

    authors： Niewiarowski S,Rucinski B,Budzynski AZ

    更新日期：1979-07-01 00:00:00

  • Influence of polymorphisms in the factor VII gene promoter on activated factor VII levels and on the risk of myocardial infarction in advanced coronary atherosclerosis.

    abstract：:In this study, we investigate the influence of three factor VII (FVII) gene polymorphisms on activated FVII levels (FVIIa), and also on the risk of myocardial infarction (MI) in patients with advanced coronary atherosclerotic disease (CAD). The -323A2 allele in the promoter is known to be associated with low FVII leve...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：10.1160/TH04-02-0130

    authors： Bozzini C,Girelli D,Bernardi F,Ferraresi P,Olivieri O,Pinotti M,Martinelli N,Manzato F,Friso S,Villa G,Pizzolo F,Beltrame F,Corrocher R

    更新日期：2004-09-01 00:00:00

  • Baboon models of acute arterial thrombosis.

    abstract：:In conclusion, we believe that the baboon offers many advantages as an experimental animal to study vascular disease, thrombus formation and dissolution, the effects of mediating variables, and the relative efficacy of therapeutic interventions. Each specific application for testing therapeutic agents may require test...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章,评审

    doi：

    authors： Hanson SR,Harker LA

    更新日期：1987-10-28 00:00:00

  • Von Willebrand's disease caused by compound heterozygosity for a substitution mutation (T1156M) in the D3 domain of the von Willebrand factor and a stop mutation (Q2470X).

    abstract：:Hereditary defects of the von Willebrand factor (VWF) gene cause von Willebrand's disease (VWD) which shows great variability dependent on the nature and location of the mutation. We here describe the characteristics of a substitution of methionine for threonine 1156 in the D3 domain of the VWF, i.e. the domain involv...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Lethagen S,Isaksson C,Schaedel C,Holmberg L

    更新日期：2002-09-01 00:00:00

  • Sympathoadrenal activation and muscarinic receptor stimulation induce acute release of tissue-type plasminogen activator but not von Willebrand factor across the human forearm.

    abstract：:We have previously shown that both mental stress and administration of the muscarinic receptor agonist methacholine induce an acute release of tissue-type plasminogen activator (t-PA) across the human forearm. There are data indicating that the regulated acute release of t-PA from the endothelium is closely interrelat...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Jern C,Selin L,Tengborn L,Jern S

    更新日期：1997-08-01 00:00:00

  • Porcine platelet von Willebrand antigen II (vW AgII): inhibitory effect on collagen-induced aggregation and comparative distribution with human platelets.

    abstract：:Von Willebrand factor (vWF) is synthesized by human endothelial cells and megakaryocytes as a large precursor, the pre-provWF, which is finally cleaved into the propolypeptide of vWF (pp-vWF) and the mature vWF. We have purified in parallel the pp-vWF and the GP IIb-IIIa from porcine platelets. The N-terminus comparat...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Royo T,Vidal M,Badimon L

    更新日期：1998-10-01 00:00:00

  • Antithrombin up-regulates AMP-activated protein kinase signalling during myocardial ischaemia/reperfusion injury.

    abstract：:Antithrombin (AT) is a protein of the serpin superfamily involved in regulation of the proteolytic activity of the serine proteases of the coagulation system. AT is known to exhibit anti-inflammatory and cardioprotective properties when it binds to heparan sulfate proteoglycans (HSPGs) on vascular cells. AMP-activated...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：10.1160/TH14-04-0360

    authors： Ma Y,Wang J,Gao J,Yang H,Wang Y,Manithody C,Li J,Rezaie AR

    更新日期：2015-02-01 00:00:00

  • Edoxaban administration following enoxaparin: a pharmacodynamic, pharmacokinetic, and tolerability assessment in human subjects.

    abstract：:Edoxaban is an oral direct factor (F)Xa inhibitor in advanced stages of clinical development. The primary objective of the present study was to assess the pharmacodynamics (PD) and safety of enoxaparin 1 mg/kg followed 12 hours (h) post-dose by edoxaban 60 mg, which is the regimen being used in the phase III study of ...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章,随机对照试验

    doi：10.1160/TH11-09-0676

    authors： Zahir H,Matsushima N,Halim AB,He L,Zhang G,Lee F,Worland V,Mendell J

    更新日期：2012-07-01 00:00:00

  • Fibrinogen gamma gene 3'-end polymorphisms and risk of venous thromboembolism in the African-American and Caucasian population.

    abstract：:Genetic determinants of venous thromboembolism (VTE) in the African-American population are poorly characterised. It was recently shown that fibrinogen gamma gene (FGG) polymorphisms 10034C>T and 9340T>C influence VTE risk in the Caucasian population. In the African-American population these polymorphisms are common, ...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Uitte de Willige S,Pyle ME,Vos HL,de Visser MC,Lally C,Dowling NF,Hooper WC,Bertina RM,Austin H

    更新日期：2009-06-01 00:00:00

  • Molecular weight measurements of low molecular weight heparins by gel permeation chromatography.

    abstract：:The molecular weight profiles of low molecular weight heparin samples have been measured by high-performance gel permeation chromatography using as calibrant the heparinase-degraded material (90/686) now established as the 1st International Reference Preparation (IRP) Low Molecular Weight Heparin for Molecular Weight ...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Mulloy B,Gee C,Wheeler SF,Wait R,Gray E,Barrowcliffe TW

    更新日期：1997-04-01 00:00:00

  • Investigation of the interaction of blood platelets with the coagulation system at the site of plug formation in vivo in man--effect of low-dose aspirin.

    abstract：:In 7 healthy volunteers, formation of thrombin (represented by fibrinopeptide A (FPA) generation, alpha-granule release (represented by beta-thromboglobulin [beta TG] release) and the generation of thromboxane B2 (TxB2) were measured in vivo in blood emerging from a template bleeding time incision. At the site of plug...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Kyrle PA,Westwick J,Scully MF,Kakkar VV,Lewis GP

    更新日期：1987-02-03 00:00:00

  • Effects of the oral, direct thrombin inhibitor dabigatran on five common coagulation assays.

    abstract：:Dabigatran is an oral, reversible thrombin inhibitor that has shown promising results in large clinical trials. Laboratory monitoring is not needed but the effects on common coagulation assays are incompletely known. Dabigatran was added to plasma from healthy subjects in the concentration range 0-1,000 μg/l and analy...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：10.1160/TH10-06-0342

    authors： Lindahl TL,Baghaei F,Blixter IF,Gustafsson KM,Stigendal L,Sten-Linder M,Strandberg K,Hillarp A,Expert Group on Coagulation of the External Quality Assurance in Laboratory Medicine in Sweden.

    更新日期：2011-02-01 00:00:00

  • In proven deep vein thrombosis, a low positive D-Dimer score is a strong negative predictor for associated malignancy.

    abstract：:D-Dimer measurements are being increasingly used for negative prediction of deep vein thrombosis (DVT). At our institution, clinical score, D-Dimer assay, plethysmography and, if necessary, Doppler ultrasound are used to secure the diagnosis. We collected the data from 100 consecutive patients proven to have DVT. We e...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：10.1160/TH03-11-0699

    authors： Rege KP,Jones S,Day J,Hoggarth CE

    更新日期：2004-06-01 00:00:00

  • A naturally occurring point mutation in the beta3 integrin MIDAS-like domain affects differently alphavbeta3 and alphaIIIbbeta3 receptor function.

    abstract：:We have investigated the effect of a new Leu196Pro mutation, identified in the MIDAS-like domain of the beta3 integrin subunit in a patient with type II Glanzmann thrombasthenia, on beta3 integrin receptor function. Expression of the mutant beta3Pro196 subunit in CHO cells, either associated with recombinant human alp...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Morel-Kopp MC,Melchior C,Chen P,Ammerlaan W,Lecompte T,Kaplan C,Kieffer N

    更新日期：2001-12-01 00:00:00

  • Heparin reverses the procoagulant properties of stimulated endothelial cells.

    abstract：:We examined the ability of unfractionated heparin to modulate the procoagulant activities of stimulated endothelial cells (EC). Confluent human venous umbilical EC were incubated with heparin before or after stimulation, then rinsed extensively to eliminate any heparin in the solution. EC, stimulated for 4 h with endo...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Cadroy Y,Gaspin D,Dupouy D,Lormeau JC,Boneu B,Sié P

    更新日期：1996-01-01 00:00:00

  • Heterozygous abnormal fibrinogen Osaka III with the replacement of gamma arginine-275 by histidine has an apparently higher molecular weight gamma-chain variant.

    abstract：:Congenitally abnormal fibrinogen Osaka III with the replacement of gamma Arg-275 by His was found in a 38-year-old female with no bleeding or thrombotic tendency. Release of fibrinopeptide(s) by thrombin or reptilase was normal, but her thrombin or reptilase time in the absence of calcium was markedly prolonged and th...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Yoshida N,Imaoka S,Hirata H,Matsuda M,Asakura S

    更新日期：1992-11-10 00:00:00

  • Low levels of the anticoagulant activity of protein C in patients with chronic renal insufficiency: an inhibitor of protein C is present in uremic plasma.

    abstract：:Twenty-nine of 54 uremic patients had low levels of protein C measured as anticoagulant activity, contrasting with normal levels measured as amidolytic activity or antigenic concentration. We demonstrate that this discrepancy is due to the presence of a soluble plasma inhibitor that interferes specifically with the an...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Faioni EM,Franchi F,Krachmalnicoff A,Valsecchi C,Viganò GL,Remuzzi G,Mannucci PM

    更新日期：1991-10-01 00:00:00

  • Isolation of peptides with anti-urokinase activity from the human placenta.

    abstract：:It is known that the fibrinolysis inhibitors in the human placenta are proteins which, in vitro, block urokinase plasminogen activation and are for this reason called urokinase inhibitors. In the present study it was found, for the first time, that a slow-molecular urokinase inhibitor also occurs in the human placenta...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Uszynski M

    更新日期：1980-02-29 00:00:00

  • Standardisation of factor VIII--V. Calibration of the 2nd International Standard for Factor VIII and von Willebrand factor activities in plasma.

    abstract：:The proposed 2nd International Standard for Factor VIII and von Willebrand Factor activities in plasma, NIBSC code 87/718, was assayed against the 1st IRP, 80/511, and against fresh normal plasma, in 21 laboratories. There were no significant differences between the various assay methods for factor VIII antigen, von W...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Heath AB,Barrowcliffe TW

    更新日期：1992-08-03 00:00:00

  • Comparison of clinical probability-adjusted D-dimer and age-adjusted D-dimer interpretation to exclude venous thromboembolism.

    abstract：:Two new strategies for interpreting D-dimer results have been proposed: i) using a progressively higher D-dimer threshold with increasing age (age-adjusted strategy) and ii) using a D-dimer threshold in patients with low clinical probability that is twice the threshold used in patients with moderate clinical probabili...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：10.1160/TH17-03-0182

    authors： Takach Lapner S,Julian JA,Linkins LA,Bates S,Kearon C

    更新日期：2017-10-05 00:00:00

  • Low molecular weight heparin therapy: is monitoring needed?

    abstract：:Recent meta-analyses indicate that low molecular weight heparins (LMWH) are more effective than unfractionated heparin (UH) in preventing and treating deep vein thrombosis. This article presents the arguments for and against the need for laboratory monitoring. At the present time, the only tests currently available fo...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章,评审

    doi：

    authors： Boneu B

    更新日期：1994-09-01 00:00:00

  • A long-lasting, plasmin-activatable thrombin inhibitor aids clot lysis in vitro and does not promote bleeding in vivo.

    abstract：:The leech protein hirudin is a potent inhibitor of thrombin, but clinical use of recombinant hirudin is restricted by haemorrhagic risks, and complicated by hirudin's rapid clearance from the circulation. We previously employed albumin fusion to slow hirudin variant 3 (HV3) clearance. In this study, we hypothesized th...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Sheffield WP,Eltringham-Smith LJ,Gataiance S,Bhakta V

    更新日期：2009-05-01 00:00:00

  • Inhibition of monocyte, lymphocyte, and neutrophil adhesion to endothelial cells by human milk oligosaccharides.

    abstract：:Excessive leukocyte infiltration causes severe tissue damage in a variety of inflammatory diseases. The initial step in leukocyte extravasation is mediated by selectins and oligosaccharides on their glycoconjugate ligands. Human milk is a rich source of lactose-derived oligosaccharides that are partly absorbed in the ...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：10.1160/TH04-01-0055

    authors： Bode L,Kunz C,Muhly-Reinholz M,Mayer K,Seeger W,Rudloff S

    更新日期：2004-12-01 00:00:00

  • Blood collection in strong acidic citrate anticoagulant used in a study of dietary influence on basal tPA activity.

    abstract：:Tissue plasminogen activator (tPA) activity in blood and blood plasma has been difficult to determine because it is unstable, especially in subjects with high levels of plasminogen activator inhibitor type 1 (PAI-1). We have attempted to stabilize the tPA activity by collecting 9 volumes of blood in 1 volume acidic ci...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Rånby M,Sundell IB,Nilsson TK

    更新日期：1989-11-24 00:00:00

  • Induction of endothelial cell adhesion molecules by serum and immunoglobulins G from a patient with vasculitis and monoclonal gammapathy: potential relevance to vasculitis.

    abstract：:Interactions between endothelial cell adhesion molecules and their beta2 integrin adhesive receptors on leukocytes are thought to play a role in the pathogenesis of inflammatory diseases and probably vasculitis. We describe a case in whom leukocytoclastic vasculitis was associated to a monoclonal immunoglobulin G2 kap...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：

    authors： Dupuy E,Herbert JM,Giraudeau V,Quere I,Zini JM,Tobelem G

    更新日期：1998-09-01 00:00:00

  • The unsialylated subpopulation of recombinant activated factor VII binds to the asialo-glycoprotein receptor (ASGPR) on primary rat hepatocytes.

    abstract：:Recombinant activated factor VII (rFVIIa; NovoSeven®) is a heterogeneously glycosylated serine protease used for treatment of haemophiliacs with inhibitors. The drug substance contains a subpopulation consisting of ~20% of rFVIIa molecules which are unsialylated and consists of carbohydrate moieties with terminally ex...

    journal_title：Thrombosis and haemostasis

    pub_type： 杂志文章

    doi：10.1160/TH10-06-0356

    authors： Seested T,Nielsen HM,Christensen EI,Appa RS

    更新日期：2010-12-01 00:00:00