Flow cytometric analysis of circulating platelet-monocyte aggregates in whole blood: methodological considerations.

abstract：:Previous studies on recombinant human soluble thrombomodulin (rsTM) from Chinese hamster ovary cells revealed that rsTM was expressed as two proteins that differed functionally in vitro due to the presence (rsTM beta) or absence (rsTM alpha) of chondroitin-4-sulfate. The current study evaluates the in vivo behavior of...

journal_title：Thrombosis and haemostasis

authors： Nawa K,Itani T,Ono M,Sakano K,Marumoto Y,Iwamoto M

更新日期：1992-03-02 00:00:00

abstract：:The mechanism of thrombin induction of tissue- and urokinase-type plasminogen activator (t-PA and u-PA) biosynthesis was investigated in cultured human fetal lung fibroblast cells, IMR-90. Northern blot analysis of total RNA from thrombin-treated cells showed marked accumulations of both t-PA and u-PA mRNA during 24 h...

journal_title：Thrombosis and haemostasis

authors： Hayakawa Y,Tazawa S,Ishikawa T,Niiya K,Sakuragawa N

更新日期：1995-08-01 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) is a potent mitogen for endothelial cells and plays a central role in angiogenesis and vasculogenesis. Therefore, VEGF and its receptors VEGFR-1 and VEGFR-2 are prime targets for anti-angiogenic intervention which is thought to be one of the most promising approaches in cancer...

journal_title：Thrombosis and haemostasis

authors： Piossek C,Thierauch KH,Schneider-Mergener J,Volkmer-Engert R,Bachmann MF,Korff T,Augustin HG,Germeroth L

更新日期：2003-09-01 00:00:00

abstract：:Atrial fibrillation (AF) is a common complication of coronary artery bypass grafting (CABG). We sought to determine the diagnostic validity of plasma biomarkers of i) inflammation (marked by interleukin-6 [IL-6] and high-sensitivity C-reactive protein [hs-CRP]), ii) extracellular matrix remodelling (matrix metalloprot...

journal_title：Thrombosis and haemostasis

authors： Kaireviciute D,Blann AD,Balakrishnan B,Lane DA,Patel JV,Uzdavinys G,Norkunas G,Kalinauskas G,Sirvydis V,Aidietis A,Lip GY

更新日期：2010-07-01 00:00:00

abstract：:Plasmin triggers chemotaxis and NF-kappa B- and AP-1-mediated proinflammatory gene expression in human peripheral monocytes (PM). Compared with macrophages and dendritic cells, PM express mainly the peroxisome proliferator-activated receptor (PPAR) gamma and traces of PPAR alpha as detected by semiquantitative RT-PCR ...

journal_title：Thrombosis and haemostasis

authors： Syrovets T,Schüle A,Jendrach M,Büchele B,Simmet T

更新日期：2002-08-01 00:00:00

abstract：:Fibrin sealant use in the United States has been limited because of the lack of a commercially available, government approved product. However, the significant need and usefulness of this material at the time of surgical operations has stimulated alternative sources of production. At the University of Virginia, the Bl...

journal_title：Thrombosis and haemostasis

authors： Spotnitz WD

更新日期：1995-07-01 00:00:00

abstract：:We aimed to determine the association between autoantibodies against apolipoprotein A-1 (anti-apoA-1 IgG) and prevalent cardiovascular (CV) disease (CVD) as well as markers of CV risk in the general population. Cross-sectional data were obtained from 6649 subjects (age 52.6 ± 10.7 years, 47.4 % male) of the population...

journal_title：Thrombosis and haemostasis

authors： Antiochos P,Marques-Vidal P,Virzi J,Pagano S,Satta N,Bastardot F,Hartley O,Montecucco F,Mach F,Waeber G,Vollenweider P,Vuilleumier N

更新日期：2016-09-27 00:00:00

abstract：:Recent progress in the understanding of thrombus formation has suggested an important role of glycoprotein (GP)VI. In contrast to its pivotal role in collagen-induced platelet activation, it has been suggested that its blockade does not induce massive bleeding tendency. To demonstrate the dissociation between inhibito...

journal_title：Thrombosis and haemostasis

authors： Matsumoto Y,Takizawa H,Nakama K,Gong X,Yamada Y,Tandon NN,Kambayashi J

更新日期：2006-08-01 00:00:00

abstract：:Hemorrhagic diathesis was observed in patients with renal insufficiency after carbenicillin at serum levels greater than 300 mug/ml. Normal coagulation factors (F. I, II, V, VII, VIII, X), normal PTT, normal platelet counts, negative ethanol gelation test (fibrin monomers) were found as well as a prolongation of throm...

journal_title：Thrombosis and haemostasis

authors： Andrassy K,Weischedel E,Ritz E,Andrassy T

更新日期：1976-08-31 00:00:00

abstract：:Undoubtedly, platelets are key elements in the regulation of thrombosis and haemostasis. Along with their primary task to prevent blood loss from injured vessels, platelets have emerged as regulators of a variety of processes in the vasculature. Multiple challenges, from the contact and adhesion to subendothelial matr...

journal_title：Thrombosis and haemostasis

authors： von Hundelshausen P,Petersen F,Brandt E

更新日期：2007-05-01 00:00:00

abstract：:Measurement of heparin ex vivo is usually with reference to standard curve prepared with a "spiked" normal human plasma pool (NHP). When the calibration curve was prepared by addition of heparin to whole blood before plasma separation, although the linear relationship was maintained the slope was increased in comparis...

journal_title：Thrombosis and haemostasis

authors： Melissari E,Thye C,Scully MF,Kakkar VV

更新日期：1989-04-25 00:00:00

abstract：:The significance of platelet beta-adrenoceptors for platelet responses to adrenergic stimuli in vivo and in vitro was studied in healthy volunteers. Low dose infusion of the beta-adrenoceptor agonist isoprenaline decreased platelet aggregability in vivo as measured by ex vivo filtragometry. Infusion of adrenaline, a m...

journal_title：Thrombosis and haemostasis

authors： Larsson PT,Wallén NH,Martinsson A,Egberg N,Hjemdahl P

更新日期：1992-12-07 00:00:00

abstract：:The structural determinants of lipopolysaccharide required for the induction of tissue factor in human umbilical vein endothelial cells were studied. Intact lipid A was essential for the induction of tissue factor whereas the incomplete lipid A precursors lipid IVA and lipid X, as well as monophosphoryl lipid A and ac...

journal_title：Thrombosis and haemostasis

authors： Moldow CF,Bach RR,Staskus K,Rick PD

更新日期：1993-10-18 00:00:00

abstract：:Heparin-induced thrombocytopenia (HIT), a relatively common complication of heparin therapy, results of platelet activation, via the receptor for the Fc domain of IgG (FcgammaRIIa), by heparin-dependent-antibodies, commonly directed against the heparin-platelet factor 4 (H-PF4) antigenic complex. Our strategy was to u...

journal_title：Thrombosis and haemostasis

authors： Khairy M,Lasne D,Brohard-Bohn B,Aiach M,Rendu F,Bachelot-Loza C

更新日期：2001-06-01 00:00:00

abstract：:Mammalian urokinase-type plasminogen activator (uPA) is produced as a stable single polypeptide chain zymogen and requires a distinct proteolytic cleavage to become an active, two-chain enzyme. In contrast, chicken uPA, both native and recombinant, is found predominantly as a two-chain, active enzyme even in the absen...

journal_title：Thrombosis and haemostasis

authors： Aimes RT,Regazzoni K,Quigley JP

更新日期：2003-02-01 00:00:00

abstract：:The turnover of recombinant pro-urokinase (Rec-pro-UK), recombinant urokinase (Rec-UK) and natural urinary urokinase (Nat-UK) was studied in rabbits and in squirrel monkeys (Saimiri sciureus). Following intravenous injection, urokinase activity disappeared rapidly from the blood. The initial disappearance rate could b...

journal_title：Thrombosis and haemostasis

authors： Collen D,De Cock F,Lijnen HR

更新日期：1984-08-31 00:00:00

abstract：:The effects on the haemostatic mechanism of oestrogen therapy, given to prevent bone loss in post-menopausal women, have been investigated. Oestriol succinate was given orally to 10 women at a level of 2 mg/day for 1 month and for a further 3 months with incremental increase of 2 mg each month. 6 of the 10 women were ...

journal_title：Thrombosis and haemostasis

authors： Davies T,Fieldhouse G,McNicol GP

更新日期：1976-04-30 00:00:00

abstract：:Following the initial report of the clinical use of plasminogen activators (PAs) in cerebrovascular thrombosis in 1958, interest in the efficacy of this approach accelerated only when the need for acute intervention in stroke was recognized. The use of PAs in acute ischemic stroke is based on the observation that appr...

journal_title：Thrombosis and haemostasis

authors： del Zoppo GJ

更新日期：1997-07-01 00:00:00

abstract：:Factor VII is a vitamin K-dependent zymogen that plays a key role in the initiation of the extrinsic pathway. A severe factor VII deficiency was identified in a 45-year old male whose plasma factor VII antigen was less than 60 ng/ml and expressed 5.2% of normal factor VII activity. DNA sequence analysis of the patient...

journal_title：Thrombosis and haemostasis

authors： Katsumi A,Matsushita T,Yamazaki T,Sugiura I,Kojima T,Saito H

更新日期：2000-02-01 00:00:00

abstract：:Emicizumab, a humanised bispecific antibody recognising factors (F) IX/IXa and X/Xa, can accelerate FIXa-catalysed FX activation by bridging FIXa and FX in a manner similar to FVIIIa. However, details of the emicizumab-antigen interactions have not been reported so far. In this study, we first showed by surface plasmo...

journal_title：Thrombosis and haemostasis

authors： Kitazawa T,Esaki K,Tachibana T,Ishii S,Soeda T,Muto A,Kawabe Y,Igawa T,Tsunoda H,Nogami K,Shima M,Hattori K

更新日期：2017-06-28 00:00:00

abstract：:In vitro and in vivo effects of adrenaline (ADR) on platelet aggregation, on platelet factor 3 (PF3) availability and on platelet factor 4 (PF4) release were studied in man. Inhibitory action of an alpha-blocker, phentolamine (PHEN) was investigated in the same conditions. The threshold concentration (TC) of ADR induc...

journal_title：Thrombosis and haemostasis

authors： Ponari O,Civardi E,Megha A,Pini M,Poti' R,Dettori AG

更新日期：1978-10-31 00:00:00

abstract：:We studied the role of various markers of lipid metabolism, hemostasis and inflammation in a two year follow-up of 3,000 patients with angina pectoris, during which time 106 patients experienced myocardial infarction or sudden coronary death. Low levels of high density lipoprotein (HDL) cholesterol and of apolipoprote...

journal_title：Thrombosis and haemostasis

authors： Bolibar I,von Eckardstein A,Assmann G,Thompson S,ECAT Angina Pectoris Study Group. European Concerted Action on Thrombosis and Disabilities.

更新日期：2000-12-01 00:00:00

abstract：:Accumulation of the newly formed 14C-cyclic adenosine 3',5'-monophosphate (cyclic AMP) was found in the P1 (1.0) fraction, i.e. a platelet plasma membrane fraction which was obtained from 14C-adenine-labeled platelets. On the other hand, total cyclic AMP as determined simultaneously was located mainly in the platelet ...

journal_title：Thrombosis and haemostasis

authors： Hashimoto S

更新日期：1983-02-28 00:00:00

abstract：:Tissue factor is the primary initiator of coagulation cascade and plays an essential role in haemostasis and thrombosis. In addition, tissue factor and coagulation proteases contribute to many cellular responses via activation of protease activated receptors. The heart is an organ with high levels of constitutive tiss...

journal_title：Thrombosis and haemostasis

authors： Antoniak S,Sparkenbaugh E,Pawlinski R

更新日期：2014-11-01 00:00:00

abstract：:Mechanisms mediating initial adsorption of coagulation proteins to live cells were investigated. Adsorption kinetics were examined under varying flow conditions using tracer-dilution techniques in perfused spherical monolayers of cells expressing tissue factor. At biologically relevant time and concentration ranges, r...

journal_title：Thrombosis and haemostasis

authors： McGee MP,Teuschler H

更新日期：1999-07-01 00:00:00

abstract：:Congenital amegakaryocytic thrombocytopaenia (CAMT) is a disorder caused by c-MPL mutations that impair thrombopoietin (TPO) signalling, resulting in a near absence of megakaryocytes (MKs). While this phenotype is consistent in adults, neonates with CAMT can present with severe thrombocytopaenia despite normal MK numb...

journal_title：Thrombosis and haemostasis

authors： Lorenz V,Ramsey H,Liu ZJ,Italiano J Jr,Hoffmeister K,Bihorel S,Mager D,Hu Z,Slayton WB,Kile BT,Sola-Visner M,Ferrer-Marin F

更新日期：2017-12-01 00:00:00

abstract：:ADP or thrombin caused a transient change in creatine phosphate (CP) content of rat platelets. At 25 degrees C the CP content decreased most 30 sec after the addition of ADP or thrombin and recovered thereafter. ATP or AMP was without effect. The rat platelets aggregated at 25 degrees C by ADP or thrombin, but the cha...

journal_title：Thrombosis and haemostasis

authors： Shibata S

更新日期：1979-08-31 00:00:00

abstract：:Venous thromboembolic (VTE) complications in children and adolescents with acute lymphoblastic leukaemia (ALL) and T or B cell lymphoblastic lymphoma (T/B cell LL) can result not only in life-threatening acute complications but also contribute to significant long-term sequelae. The PREVAPIX-ALL study is an open-label ...

journal_title：Thrombosis and haemostasis

authors： O'Brien SH,Li D,Mitchell LG,Hess T,Zee P,Yee DL,Newburger JW,Sung L,Rodriguez V

更新日期：2019-05-01 00:00:00

abstract：:Platelet adhesion and aggregation at the sites of vascular injury are key events for thrombosis and haemostasis. It has been well demonstrated that interaction between glycoprotein (GP) Ibα and von Willebrand factor (VWF) initiates platelet adhesion and contributes to platelet aggregation, particularly at high shear. ...

journal_title：Thrombosis and haemostasis

authors： Lei X,Reheman A,Hou Y,Zhou H,Wang Y,Marshall AH,Liang C,Dai X,Li BX,Vanhoorelbeke K,Ni H

更新日期：2014-02-01 00:00:00

abstract：:Increased procoagulant activity in the alveolar compartment and uncontrolled inflammation are hallmarks of the acute respiratory distress syndrome (ARDS). Here, we investigated whether the contact phase system of coagulation is activated and may regulate inflammatory responses in human lungs. Components of the contact...

journal_title：Thrombosis and haemostasis

authors： Hess R,Wujak L,Hesse C,Sewald K,Jonigk D,Warnecke G,Fieguth HG,de Maat S,Maas C,Bonella F,Preissner KT,Weiss B,Schaefer L,Kuebler WM,Markart P,Wygrecka M

更新日期：2017-10-05 00:00:00